Cross-Reference to Related Applications

This application claims priority under 35 U.S.C. § 119(e) to U.S. Application No. 62/487,812, filed April 20, 2017, which is incorporated by reference in its entirety.

Statement Regarding Sequence Listing

The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is ATYR_131_01WO_ST25.txt. The text file is about 276 KB, was created on April 18, 2018, and is being submitted electronically via EFS-Web.

Background

Technical Field

Embodiments of the present disclosure relate to therapies, including combination therapies, for the treatment of lung inflammation, including interstitial lung diseases (ILDs), which include the use of at least one histidyl-tRNA synthetase (HRS) polypeptide or an expressible polynucleotide that encodes the HRS polypeptide, alone or in combination with at least one immunomodulatory agent.

Description of the Related Art

Interstitial lung diseases (ILDs) are group of heterogeneous disorders that primarily affect the lung interstitium in which inflammation is a predominant underlying mechanism. Within the ILD designation, there are a number of fibrotic lung conditions that are generally recognized as having a measureable inflammatory component involving both innate and adaptive immune mechanisms that contribute to pathogenesis at several levels.

Patients with ILD often suffer from progressive, debilitating respiratory symptoms, and experience significant morbidity and increased mortality compared to the general population. As a group, these conditions constitute a high unmet medical need, for which there are few effective treatments which don't have significant unwanted side effects.

BRIEF SUMMARY

Embodiments of the present disclosure relate, in pertinent part, to therapeutic compositions, comprising:

(a) a histidyl-tRNA synthetase (HRS) polypeptide, or an expressible polynucleotide that encodes the HRS polypeptide; and

(b) an immunomodulatory agent. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table HI, Table H2, and Table H4.

In some embodiments, the HRS polypeptide is 500-506 amino acids in length and is at least 90% identical to SEQ ID NO:8 (HRS(l-506)) or 9 (HRS(2-506)) and lacks residues 507-509 of SEQ ID NO: l. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO:8 (HRS(l-506)). In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO:9 (HRS(2-506)).

In some embodiments, the HRS polypeptide is fused to a heterologous polypeptide. In some embodiments, the heterologous polypeptide comprises an Fc region, to form an HRS-Fc fusion polypeptide.

In some embodiments, the HRS-Fc fusion polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table H8. In some embodiments, the HRS polypeptide at least about 80%, 85%, 90%, or 95% pure on a protein basis and less than about 5% aggregated.

In some embodiments, (a) is an expressible polynucleotide that encodes the HRS polypeptide, optionally a modified mRNA polynucleotide, which optionally comprises one or more non-natural basis and/or non-natural internucleotide linkages.

In some embodiments, the HRS polypeptide has a non-canonical activity, optionally an antiinflammatory activity.

In some embodiments, the immunomodulatory agent is selected from one or more of pirfenidone, nintedanib, a sphingosine-1 -phosphate (SIP) and/or S IP receptor (S1PR) modulator, a steroid optionally a glucocorticoid, a calcineurin inhibitor, a mechanistic target of rapamycin (mTOR) inhibitor, an indoleamine-pyrrole 2,3-dioxygenase (IDO) inhibitor, an inosine-5' -monophosphate dehydrogenase (IMPDH) inhibitor, a cytokine and/or cytokine receptor inhibitor, a B cell receptor inhibitor, a kinase inhibitor, and a cytostatic agent optionally methotrexate.

In some embodiments, the SIP and/or S 1PR modulator is selected from amiselimod (S 1PR antagonist;), fingolimod (S lPRi functional antagonist), sonepcizumab (S IP-specific monoclonal antibody), KRP203 (SlPRi agonist), SEW2871 (S lPRi agonist), siponimod (S lPRi and SlPRs modulator), RPC1063 (S lPRi modulator), ONO-4641 (SlPRi and S1PR ₅ agonist), JTE-013 (S1PR ₂ antagonist), GSK2018682 (SlPRi agonist), ponesimod (S lPRi agonist), suramin (selective SIPR3 and SIPR5 antagonist), VPC23019 (aryl-amide analogs; competitive SlPRi and S IPR3 antagonists); and W146 (selective SlPRi antagonist), an antisense or RNAi agent targeted against an S1PR, and an antibody or antigen-binding fragment or small molecule that specifically binds S IP and/or an S1PR, optionally wherein the amiselimod is at a dosage unit that ranges from about 0.1 mg to about 10 mg, or a dosage unit of about, no more than about, or at least about 0.1, 0.2, 0.3, 0.337, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg, or optionally wherein the amiselimod is at a dosage unit that ranges from about 0.1 mg/kg to about 10 mg/kg, or a dosage unit of about, no more than about, or at least about 0.1, 0.2, 0.3, 0.337, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg/kg.

In some embodiments, the steroid is selected from betamethasone, budesonide, Cortisol (hydrocortisone), cortisone, deflazacort, deoxycorticosterone, dexamethasone, fludrocortisone, hydrocortisone, methylprednisolone, prednisone, prednisolone, and triamcinolone.

In some embodiments, the calcineurin inhibitor is selected from cyclosporin, pimecrolimus, tacrolimus, an antisense or RNAi agent targeted against calcineurin or a subunit thereof, and an antibody or antigen-binding fragment or small molecule that specifically binds calcineurin or a subunit thereof.

In some embodiments, the mTOR inhibitor is an ATP -competitive mTOR kinase inhibitor, an mTORCl/mTORC2 dual inhibitor, and/or an mTOR/PBK dual inhibitor, or wherein the mTOR inhibitor is selected from one or more of everolimus, rapamycin, deforolimus, temsirolimus, dactolisib, BGT226, SF1126, PKI-587, NVPBE235, sapanisertib, AZD8055, AZD2014, antisense or RNAi agent targeted against mTOR, and an antibody or antigen-binding fragment or small molecule that specifically binds mTOR.

In some embodiments, the IDO inhibitor is selected from indoximod (NLG-8189), 1-methyl- tryptophan (1MT), β-Carboline (norharmane; 9H-pyrido[3,4-b]indole), rosmarinic acid, and epacadostat, an antisense or RNAi agent targeted against IDO, and an antibody or antigen-binding fragment or small molecule that specifically binds IDO.

In some embodiments, the IMPDH inhibitor is selected from one or more of mycophenolic acid (mycophenolate mofetil), ribavirin, and 6TGMP (6-thioguanine monophosphate), an antisense or RNAi agent targeted against IMPDH, and an antibody or antigen-binding fragment or small molecule that specifically binds IMPDH.

In some embodiments, the cytokine inhibitor is an inhibitor of a cytokine selected from one or more of interleukin-1 (IL-1) including IL-la and IL-Ιβ, interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin- 11 (IL-11), interleukin- 12 (IL-12), interleukin- 17 (IL-17), interleukin- 18 (IL-18), interleukin-20 (IL-20), interleukin-33 (IL-33), tumor necrosis factor (TNF), interferon gamma (IFN-gamma), transforming growth factor-β (TGF-β), and granulocyte-macrophage colony stimulating factor (GM-CSF), and/or a cytokine receptor selected from one or more of IL-1R, IL-6R, IL-8R, IL-l lR, IL-12R, IL-17R, IL-18R, IL-20R, ST2 (Interleukin 1 receptor-like 1, ILIRLI), a TNFR such as TNFRl, interferon-gamma receptor (IFNGR), and a TGF-β receptor such as TGFβRl (ALK5) or TGFβR2, and further wherein the cytokine and/or cytokine receptor inhibitor is selected from an antisense or RNAi agent targeted against the cytokine and/or cytokine receptor, and an antibody or antigen-binding fragment or small molecule that specifically binds the cytokine or cytokine receptor.

In some embodiments, the cytokine and/or cytokine receptor inhibitor is selected from one or more of adalimumab, anakinra, basiliximab, canakinumab, certolizumab, daclizumab, etanercept, golimumab, infliximab, ixekizumab, mepolizumab, reslizumab, rilonacept, secukinumab, serilumab, sirukumab, tocilizumab, and ustekinumab.

In some embodiments, the kinase inhibitor is an inhibitor of a kinase selected from one or more of a Janus kinase (JAK, including JAK1, JAK2, JAK3, TYK2), epidermal growth factor receptor (EGFR), a receptor tyrosine-protein kinase erbB-2 (Her2/neu, or ERBB2), Bcr-Abl, c-SRC, a Mitogen-activated protein kinase (MAP) kinase, anaplastic lymphoma kinase (ALK), spleen tyrosine kinase (SYK), Button's tyrosine kinase (BTK), a vascular endothelial growth factor (VEGF), a vascular endothelial growth factor receptor (VEGFR, including VEGFR1, VEGFR2, VEGFR3), a fibroblast growth factor receptor (FGFR), B-Raf, RET proto-oncogene, a platelet-derived growth factor receptor (PDGF-R), a tropomyosin receptor kinase (Trk, including TrkA, TrkB, TrkC), and c- Met, and further wherein the kinase inhibitor is selected from an antisense or RNAi agent targeted against the kinase, and an antibody or antigen-binding fragment or small molecule that specifically binds the kinase.

In some embodiments, the kinase inhibitor is selected from or more of nintedanib, baricitinib, fedratinib, filgotinib, gandotinib, lestaurtinib, momelotinib, pacritinib, peficitinib, ruxolitinib, tofacitinib, padacitinib, afatinib, axitinib, bosutinib, cetuximab, cobimetinib, crizotinib, cabozantinib, dasatinib, entrectinib, erlotinib, fostamatinib, gefitinib, ibrutinib, imatinib, lapatinib, lenvatinib, mubritinib, neratinib, nilotinib, pazopanib, pegaptanib, sorafenib, sunitinib, SU6656, toceranib, vandetanib, vatalanib, and vemurafenib.

In some embodiments, the B cell receptor inhibitor is selected from an antisense or RNAi agent targeted against CD20, and an antibody or antigen-binding fragment or small molecule that specifically binds CD20, or wherein the B cell receptor inhibitor is optionally selected from one or more of ibritumomab tiuxetan, obinutuzumab, ocaratuzumab, ocrelizumab, rituximab, tositumomab, and veltuzumab.

In some embodiments, the antisense agent is about 10-40 bases in length, and is optionally selected from a morpholino oligonucleotide (PMO), a peptide nucleic acid (PNA), a 2' O-methyl phosphorothioate oligonucleotide, a tricyclo-phosphorothioate oligonucleotide, and a locked nucleic acid (LNA).

In some embodiments, the antisense agent specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes the target protein, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3' untranslated region (UTR), and a polyadenylation signal sequence.

In some embodiments, the RNAi agent comprises a sense strand that is substantially identical to an mRNA target sequence that encodes the target protein, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes the target protein, and optionally wherein the RNAi agent is a double-stranded short-interfering RNA (siRNA) oligonucleotide, or optionally wherein the RNAi agent, optionally an siRNA oligonucleotide, is encoded by a viral vector.

In some embodiments, the antibody or antigen-binding fragment thereof is a monoclonal antibody, optionally a humanized antibody, or optionally an Fv fragment or a single chain Fv (sFv) polypeptide.

In some embodiments, the composition has a purity of at least about 80%, 85%, 90%, 95%, 98%, or 99% on a protein basis or a weight-weight basis and is substantially aggregate-free.

In some embodiments, the composition is substantially endotoxin-free.

Certain compositions comprise lipid nanoparticles.

In some embodiments, the composition is in a syringe, optionally an injectable syringe. In some embodiments, the composition is a capsule, for example, an oral capsule.

Also included are methods of treating lung inflammation in a subject in need thereof, comprising administering to the subject

(a) a histidyl-tRNA synthetase (HRS) polypeptide, or an expressible polynucleotide that encodes the HRS polypeptide; and

(b) an immunomodulatory agent.

In some embodiments, (a) and (b) are administered separately, and are optionally defined according as described herein. In some embodiments, (a) and (b) are administered together, optionally as a therapeutic composition described herein.

In some embodiments, the HRS polypeptide comprises an Fc region, to form an HRS-Fc fusion polypeptide, for example, wherein the HRS-Fc fusion polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table H8. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or

HRS ^FC1 )

In some embodiments, the immunomodulatory agent alters one or more pharmacokinetic characteristics of the HRS polypeptide relative to the HRS polypeptide alone. In certain embodiments, the one or more altered pharmacokinetic characteristics of the HRS polypeptide are increased serum half-life, increased bioavailability, increased exposure (AUC), increased serum concentration, and/or decreased clearance.

In some embodiments, the immunomodulatory agent is pirfenidone or nintedanib.

In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or HRSF ^C1 , and the immunomodulatory agent is pirfenidone. In some embodiments, the pirfenidone increases the serum concentration of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone In some embodiments, the pirfenidone is administered at an individual dosage unit that ranges from about 50 to about 1000 mg, or an individual dosage unit of about no more than about, or at least about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, or 1000 mg, optionally in 1, 2, or 3 capsules for oral dosing.

In some embodiments, the dosage of pirfenidone is administered at a daily dosage unit that ranges from about 100 to about 4000 mg/day, or a daily dosage unit of about, no more than about, or at least about 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 3100, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, or 4000 mg/day, optionally in about 1, 2, 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing.

In some embodiments, the pirfenidone is administered at an individual dosage unit of about 800 mg (e.g., 801 mg), optionally as three -267 mg capsules for oral dosing, taken as three capsules per individual dosage. In some embodiments, the pirfenidone is administered at daily dosage unit of about 2400 mg/day (e.g., 2403 mg/day), optionally as nine -267 mg capsules for oral dosing three times daily, taken as three capsules per individual dosage.

In some embodiments, the nintedanib is administered at an individual dosage unit that ranges from about 10 to about 500 mg, or an individual dosage unit of about, no more than about, or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, or 500 mg, optionally in about 1, 2, or 3 capsules.

In some embodiments, the nintedanib is administered at a daily dosage unit that ranges from about 20 to about 1000 mg/day, or a daily dosage unit of about, no more than about, or at least about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000 mg/day, optionally in about 1, 2, 3, 4, 5, or 6 capsules. In some embodiments, the nintedanib is administered at a daily dosage unit that ranges from about 100 to 150 mg, or ranges from about 200 to 300 mg/day, optionally for a once or twice daily dosage. In some embodiments, the nintedanib is administered at a daily dosage unit of about 100 or 150 mg, or about 200 to 300 mg/day, optionally for a once or twice daily dosage.

In some embodiments, the subject has or is risk for having an interstitial lung disease (ILD). In some embodiments, the ILD is idiopathic or associated with a connective tissue disease, an autoimmune disease, exposure to inhaled substances or drug(s), an infection, or a malignancy.

In some embodiments, the ILD is selected from or is associated with one or more of idiopathic interstitial pneumonia, idiopathic pulmonary fibrosis, sarcoidosis, Hammann-Rich syndrome, Antisynthetase syndrome, idiopathic eosinophilic pneumonia, alveolar hemorrhage syndrome, pulmonary alveolar proteinosis, asbestosis, silicosis, berylliosis, rheumatoid arthritis, lupus erythematosus, chronic graft vs host disease with pulmonary involvement, sclerosis (systemic) or scleroderma, polymyositis, dermatomyositis, chronic pulmonary disease, asthma, bronchitis (respiratory bronchitis), pneumonia, hypersensitivity pneumonitis, chronic hypersensitivity pneumonia, respiratory distress syndrome, Still's disease, acute lung injury, microscopic polyangitis, pulmonary edema, pulmonary Langerhans cell histiocytosis, acute inhalational exposures, drug- induced lung disease, desquamative interstitial pneumonia, and/or cystic fibrosis.

In some embodiments, the ILD is associated with one or more of Surfactant-Protein-B Deficiency (Mutations in SFTPB), Surfactant-Protein-C Deficiency (Mutations in SFTPC), ABCA3- Deficiency (Mutations in ABCA3), Brain Lung Thyroid Syndrome (Mutations in TTF1), or Congenital Pulmonary Alveolar Proteinosis (Mutations in CSFR2A, CSFR2B), Alveolar Capillary Dysplasia (Mutations in FoxFl), Mutations in telomerase reverse transcriptase (TERT), Mutations in telomerase RNA component (TERC), Mutations in the regulator of telomere elongation helicase 1 (RTEL1), and/or Mutations in poly(A)-specific ribonuclease (PARN).

In some embodiments, the drug(s) are selected from one or more of antibiotics,

chemotherapeutic agents, antiarrhythmic agents, and statins. In some embodiments, the infection is selected from one or more of atypical pneumonia, Pneumocystis pneumonia (PCP), tuberculosis, Chlamydia trachomatis, and Respiratory Syncytial Virus (RSV), cryptogenic organizing pneumonia. In some embodiments, the malignancy is lymphangitic carcinomatosis or lymphoma.

In some embodiments, the subject in need thereof has a condition selected from one or more of atopic asthma, non-atopic asthma, allergic asthma, atopic bronchial IgE-mediated asthma, bronchial asthma, essential asthma, true asthma, intrinsic asthma caused by pathophysiologic disturbances, extrinsic asthma caused by environmental factors, essential asthma of unknown or inapparent cause, non-atopic asthma, bronchitic asthma, emphysematous asthma, exercise-induced asthma, allergen induced asthma, cold air induced asthma, occupational asthma, infective asthma caused by bacterial, fungal, protozoal, or viral infection, non-allergic asthma, incipient asthma, wheezy infant syndrome and bronchiolytis, chronic or acute bronchoconstriction, chronic bronchitis, small airways obstruction, and emphysema.

In some embodiments, the subject in need thereof has an obstructive or inflammatory airway disease. In some embodiments, the obstructive or inflammatory airways disease is selected from one or more of chronic eosinophilic pneumonia, chronic obstructive pulmonary disease (COPD), COPD that includes chronic bronchitis, pulmonary emphysema or dyspnea, COPD that is characterized by irreversible, progressive airways obstruction, and acute respiratory distress syndrome (ARDS).

In some embodiments, the subject in need thereof has a condition related to exacerbation of airways hyper-reactivity consequent to other drug therapy, airway disease that is associated with pulmonary hypertension, bronchitis or acute bronchitis, acute laryngotracheal bronchitis, arachidic bronchitis, catarrhal bronchitis, croupus bronchitis, dry bronchitis, infectious asthmatic bronchitis, productive bronchitis, staphylococcus or streptococcal bronchitis, vesicular bronchitis, acute lung injury, bronchiectasis or cylindric bronchiectasis, sacculated bronchiectasis, fusiform bronchiectasis, capillary bronchiectasis, cystic bronchiectasis, dry bronchiectasis, or follicular bronchiectasis.

In some embodiments, the subject in need thereof has an Ashcroft score of 1, 2, 3, 4, 5, 6, 7, or 8.

Certain embodiments increase the life expectancy of the subject in need thereof, optionally by about or at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 or more years.

Certain embodiments improve one or more of the clinical symptoms or parameters of the lung inflammation in the subject in need thereof.

In some embodiments, the one or more clinical symptoms or parameters are selected from one or more of lung fibrosis, inflammatory cell infiltrates in the lung, respiratory function, and body weight.

Certain embodiments improve lung fibrosis in the subject in need thereof by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, optionally as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more.

Certain embodiments improve lung fibrosis in the subject in need thereof as measured by a reduced Ashcroft score, optionally an Ashcroft score that is reduced by 1, 2, 3, 4, 5, 6, 7, or 8 grades relative to an earlier score.

Certain embodiments reduce inflammatory cell infiltrates in the lung by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, optionally as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more. Certain embodiments improve respiratory function by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, optionally as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more. In some embodiments, the improved respiratory function is selected from one or more of increased expiration time, increased inspiration time, decreased peak expiratory flow, decreased peak inspiratory flow, decreased respiratory minute volume (RMV), and decreased respiratory rate.

Also included are patient care kits, comprising:

(a) a histidyl-tRNA synthetase (HRS) polypeptide, or an expressible polynucleotide that encodes the HRS polypeptide; and

(b) an immunomodulatory agent.

In certain patient care kits, (a) and (b) are in separate compositions, and are optionally defined as described herein. In some patient care kits, (a) and (b) are in the same composition, optionally as a therapeutic composition as described herein.

In some embodiments, the immunomodulatory agent is pirfenidone or nintedanib.

In some embodiments, the pirfenidone is at an individual dosage unit that ranges from about 50 to about 1000 mg (optionally in about 1, 2, or 3 capsules for oral dosing), or an individual dosage unit of about no more than about, or at least about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, or 1000 mg (optionally in 1, 2, or 3 capsules for oral dosing).

In some embodiments, the pirfenidone is at a daily dosage unit that ranges from about 100 to about 4000 mg/day (optionally in about 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing), or a daily dosage unit of about, no more than about, or at least about 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 3100, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, or 4000 mg/day (optionally in about 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing).

In specific embodiments, the pirfenidone is at an individual dosage unit of about 800 mg (e.g., 801 mg), for example, as three -267 mg capsules for oral dosing, taken as three capsules per individual dosage. In specific embodiments, the pirfenidone is at daily dosage unit of about 2400 mg/day (e.g., 2403 mg/day), for example, as nine -267 mg capsules for oral dosing three times daily, taken as three capsules per individual dosage.

In some embodiments, the nintedanib is at an individual dosage unit that ranges from about 10 to about 500 mg (optionally in about 1, 2, or 3 capsules), or an individual dosage unit of about, no more than about, or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, or 500 mg (optionally in about 1, 2, or 3 capsules).

In some embodiments, the nintedanib is at a daily dosage unit that ranges from about 20 to about 1000 mg/day (optionally in about 1, 2, 3, 4, 5, or 6 capsules), or a daily dosage unit of about, no more than about, or at least about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000 mg/day (optionally in about 1, 2, 3, 4, 5, or 6 capsules).

In some embodiments, the nintedanib is at a daily dosage unit that ranges from about 100 to 150 mg, or ranges from about 200 to 300 mg/day, optionally for a once or twice daily dosage. In some embodiments, the nintedanib is at a dosage unit of about 100 or 150 mg, or about 200 to 300 mg/day, optionally for a once or twice daily dosage.

Also included are methods of altering one or more pharmacokinetic characteristics of an HRS-Fc fusion polypeptide in a subject, comprising administering to the subject the HRS-Fc fusion polypeptide, or an expressible polynucleotide that encodes the HRS-Fc fusion polypeptide, in combination with pirfenidone. In some embodiments, HRS-Fc fusion polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table H8. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or HRS ^FC1 )

Also included are methods of treating lung inflammation (as described herein) in a subject in need thereof, comprising administering to the subject an HRS-Fc fusion polypeptide, or an expressible polynucleotide that encodes the HRS-Fc fusion polypeptide. In some embodiments, the HRS-Fc fusion polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table H8. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or HRS ^FC1 ). BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 shows BAL Fluid Cell Counts. Individual cell counts are shown with a line indicating the group mean: 'no tx" indicates no treatment, "Veh" indicates treatment with Vehicle, "Dex." indicates treatment with dexamethasone, "Ninte." indicates treatment with Nintedanib, and "TA" indicates treatment with Test Article. Statistical comparisons were conducted by 1-way ANOVA within PO or IV treatment groups followed by Dunnett's post-hoc test. *p < 0.05, **p < 0.01 vs. IV Vehicle.

Figure 2 shows Histological Fibrosis (Ashcroft) Scores. Mean data from all fields scored within each group are shown (+ SEM): 'no tx" indicates no treatment, "Veh" indicates treatment with Vehicle, "Dex." indicates treatment with dexamethasone, "Ninte." indicates treatment with

Nintedanib, and "TA" indicates treatment with Test Article. Statistical comparisons were conducted by 1-way ANOVA within PO or IV treatment groups followed by Dunnett's post-hoc test. *p < 0.05, **p < 0.01 vs. respective vehicle.

Figure 3 shows Respiratory Minute Volume (RMV) on Day 15. Individual RMVs are shown with a line indicating the group mean: "Veh" indicates treatment with Vehicle, "Ninted." indicates treatment with Nintedanib, and "TA" indicates treatment with Test Article. Test Article dose is indicated in parentheses. IV groups were compared by 1-way ANOVA, PO groups by t test. *p < 0.05, ** p < 0.01, ***p < 0.001.

Figure 4 shows Histological Fibrosis (Ashcroft) Scores. Mean data from all fields scored within each group are shown (+ SEM): "Veh" indicates treatment with Vehicle, "Ninted." indicates treatment with Nintedanib, and "TA" indicates treatment with Test Article. Test Article dose is indicated in parentheses. Statistical comparisons were conducted by t-test within PO or IV treatment groups. *p < 0.05 vs. IV vehicle.

Figure 5 shows Interstitial/ Alveolar Inflammatory Cell Infiltrates. Individual mean scores within each group are shown: "Veh" indicates treatment with Vehicle, "Ninted." indicates treatment with Nintedanib, and "TA" indicates treatment with Test Article. Test Article dose is indicated in parentheses. Statistical comparisons were conducted by t-test within PO or IV treatment groups. *p < 0.05 vs. IV vehicle.

Figure 6 shows mean serum HRS ^FC1 levels in pMol (+ SEM): for each group terminated on Day 22 of the study: Statistical comparisons were conducted by t-test ** p < 0.01, ***p < 0.001, ****p < 0.0005 between vehicle (Veh) in combination with the Test Article, compared to the groups treated with Nintedanib in combination with the Test Article, or Pirfenidone in combination with the Test Article respectively. DETAILED DESCRIPTION

The practice of the present invention will employ, unless indicated specifically to the contrary, conventional methods of molecular biology and recombinant DNA techniques within the skill of the art, many of which are described below for the purpose of illustration. Such techniques are explained fully in the literature. See, e.g., Sambrook, et al., Molecular Cloning: A Laboratory Manual (3rd Edition, 2000); DNA Cloning: A Practical Approach, vol. I & II (D. Glover, ed.);

Oligonucleotide Synthesis (N. Gait, ed., 1984); Oligonucleotide Synthesis: Methods and Applications (P. Herdewijn, ed., 2004); Nucleic Acid Hybridization (B. Hames & S. Higgins, eds., 1985); Nucleic Acid Hybridization: Modern Applications (Buzdin and Lukyanov, eds., 2009); Transcription and Translation (B. Hames & S. Higgins, eds., 1984); Animal Cell Culture (R. Freshney, ed., 1986); Freshney, R.I. (2005) Culture of Animal Cells, a Manual of Basic Technique, 5th Ed. Hoboken NJ, John Wiley & Sons; B. Perbal, A Practical Guide to Molecular Cloning (3rd Edition 2010); Farrell, R., RNA Methodologies: A Laboratory Guide for Isolation and Characterization (3rd Edition 2005). Poly(ethylene glycol), Chemistry and Biological Applications, ACS, Washington, 1997; Veronese, F., and J.M. Harris, Eds., Peptide and protein PEGylation, Advanced Drug Delivery Reviews, 54(4) 453- 609 (2002); Zalipsky, S., et al., "Use of functionalized Poly(Ethylene Glycols) for modification of polypeptides" in Polyethylene Glycol Chemistry: Biotechnical and Biomedical Applications.

Definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the disclosure belongs. Although any methods, materials, compositions, reagents, cells, similar or equivalent similar or equivalent to those described herein can be used in the practice or testing of the subject matter of the present disclosure, preferred methods and materials are described. All publications and references, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference in their entirety as if each individual publication or reference were specifically and individually indicated to be incorporated by reference herein as being fully set forth. Any patent application to which this application claims priority is also incorporated by reference herein in its entirety in the manner described above for publications and references.

For the purposes of the present disclosure, the following terms are defined below.

The articles "a" and "an" are used herein to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element.

By "about" is meant a quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by as much as 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1% to a reference quantity, level, value, number, frequency, percentage, dimension, size, amount, weight or length. An "antagonist" or "inhibitor" refers to biological structure or chemical agent that interferes with or otherwise reduces the physiological action of another agent or molecule. In some instances, the antagonist specifically binds to the other agent or molecule. Included are full and partial antagonists.

An "agonist" refers to biological structure or chemical agent that increases or enhances the physiological action of another agent or molecule. In some instances, the agonist specifically binds to the other agent or molecule. Included are full and partial agonists.

The term "anergy" refers to the functional inactivation of a T cell, or B cell response to re- stimulation by antigen.

As used herein, the term "amino acid" is intended to mean both naturally occurring and non- naturally occurring amino acids as well as amino acid analogs and mimetics. Naturally occurring amino acids include the 20 (L)-amino acids utilized during protein biosynthesis as well as others such as 4-hydroxyproline, hydroxylysine, desmosine, isodesmosine, homocysteine, citrulhne and ornithine, for example. Non-naturally occurring amino acids include, for example, (D)-amino acids, norleucine, norvaline, p-fluorophenylalanine, ethionine and the like, which are known to a person skilled in the art. Amino acid analogs include modified forms of naturally and non-naturally occurring amino acids. Such modifications can include, for example, substitution or replacement of chemical groups and moieties on the amino acid or by derivatization of the amino acid. Amino acid mimetics include, for example, organic structures which exhibit functionally similar properties such as charge and charge spacing characteristic of the reference amino acid. For example, an organic structure which mimics Arginine (Arg or R) would have a positive charge moiety located in similar molecular space and having the same degree of mobility as the e-amino group of the side chain of the naturally occurring Arg amino acid. Mimetics also include constrained structures so as to maintain optimal spacing and charge interactions of the amino acid or of the amino acid functional groups. Those skilled in the art know or can determine what structures constitute functionally equivalent amino acid analogs and amino acid mimetics.

As used herein, a subject "at risk" of developing a disease, or adverse reaction may or may not have detectable disease, or symptoms of disease, and may or may not have displayed detectable disease or symptoms of disease prior to the treatment methods described herein. "At risk" denotes that a subject has one or more risk factors, which are measurable parameters that correlate with development of a disease, as described herein and known in the art. A subject having one or more of these risk factors has a higher probability of developing disease, or an adverse reaction than a subject without one or more of these risk factor(s).

By "coding sequence" is meant any nucleic acid sequence that contributes to the code for the polypeptide product of a gene. By contrast, the term "non-coding sequence" refers to any nucleic acid sequence that does not directly contribute to the code for the polypeptide product of a gene. The term "binding" refers to a direct association between two molecules, due to, for example, covalent, electrostatic, hydrophobic, and ionic and/or hydrogen-bond interactions, including interactions such as salt bridges and water bridges.

The term "clonal deletion" refers to the deletion (e.g., loss, or death) of auto-reactive T-cells. Clonal deletion can be achieved centrally in the thymus, or in the periphery, or both.

Throughout this disclosure, unless the context requires otherwise, the words "comprise," "comprises," and "comprising" will be understood to imply the inclusion of a stated step or element or group of steps or elements but not the exclusion of any other step or element or group of steps or elements.

By "consisting of is meant including, and limited to, whatever follows the phrase "consisting of." Thus, the phrase "consisting of indicates that the listed elements are required or mandatory, and that no other elements may be present. By "consisting essentially of is meant including any elements listed after the phrase, and limited to other elements that do not interfere with or contribute to the activity or action specified in the disclosure for the listed elements. Thus, the phrase "consisting essentially of indicates that the listed elements are required or mandatory, but that other elements are optional and may or may not be present depending upon whether or not they materially affect the activity or action of the listed elements.

The term "endotoxin free" or "substantially endotoxin free" relates generally to compositions, solvents, and/or vessels that contain at most trace amounts (e.g., amounts having no clinically adverse physiological effects to a subject) of endotoxin, and preferably undetectable amounts of endotoxin. Endotoxins are toxins associated with certain micro-organisms, such as bacteria, typically gram- negative bacteria, although endotoxins may be found in gram-positive bacteria, such as Listeria monocytogenes. The most prevalent endotoxins are lipopoly saccharides (LPS) or lipo-oligo- saccharides (LOS) found in the outer membrane of various Gram-negative bacteria, and which represent a central pathogenic feature in the ability of these bacteria to cause disease. Small amounts of endotoxin in humans may produce fever, a lowering of the blood pressure, and activation of inflammation and coagulation, among other adverse physiological effects.

Therefore, in pharmaceutical production, it is often desirable to remove most or all traces of endotoxin from drug products and/or drug containers, because even small amounts may cause adverse effects in humans. A depyrogenation oven may be used for this purpose, as temperatures in excess of 300°C are typically required to break down most endotoxins. For instance, based on primary packaging material such as syringes or vials, the combination of a glass temperature of 250°C and a holding time of 30 minutes is often sufficient to achieve a 3 log reduction in endotoxin levels. Other methods of removing endotoxins are contemplated, including, for example, chromatography and filtration methods, as described herein and known in the art.

Endotoxins can be detected using routine techniques known in the art. For example, the Limulus Amoebocyte Lysate assay, which utilizes blood from the horseshoe crab, is a very sensitive assay for detecting presence of endotoxin. In this test, very low levels of LPS can cause detectable coagulation of the limulus lysate due a powerful enzymatic cascade that amplifies this reaction. Endotoxins can also be quantitated by enzyme-linked immunosorbent assay (ELISA). To be substantially endotoxin free, endotoxin levels may be less than about 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.08, 0.09, 0.1, 0.5, 1.0, 1.5, 2, 2.5, 3, 4, 5, 6, 7, 8, 9, or 10 EU/mg of active compound. Typically, 1 ng lipopoly saccharide (LPS) corresponds to about 1-10 EU.

As used herein, the terms "contacting a cell", "introducing" or "delivering" include delivery of the agents described herein (e.g., polypeptide agents, polynucleotide agents) into a cell by methods routine in the art, e.g., transfection (e.g., liposome, calcium-phosphate, polyethyleneimine), electroporation (e.g., nucleofection), microinjection) or administration to a subject.

The terms "cell penetrating peptide" (CPP) or "a peptide moiety which enhances cellular uptake" are used interchangeably and refer to cationic cell penetrating peptides, also called "transport peptides", "carrier peptides", or "peptide transduction domains." In some embodiments, the peptides have the capability of inducing cell (e.g., muscle cell) penetration within about or at least about 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% of cells of a given cell culture population and allow macromolecular translocation within multiple tissues (e.g., muscle tissues) in vivo upon systemic or other form of administration. In some embodiments, the CPPs are of the

formula -[(C(0)CHR'NH) _m ]R' ' wherein R' is a side chain of a naturally occurring amino acid or a one- or two-carbon homolog thereof , R" is selected from Hydrogen or acyl, and m is an integer up to 50. Additional CPPs are well-known in the art and are disclosed, for example, in U.S. Application No. 2010/0016215, which is incorporated by reference in its entirety. In some embodiments, m is an integer selected from 1 to 50 where, when m is 1, the moiety is a single amino acid or derivative thereof. Any of the polynucleotide agents (e.g., antisense, RNAi agents) described herein can be conjugated to a CPP, for example, to improve uptake into target cells, e.g., muscle cells.

The term "half maximal effective concentration" or "EC50" refers to the concentration of an agent (e.g., HRS polypeptide, or other agent) as described herein at which it induces a response halfway between the baseline and maximum after some specified exposure time; the EC50 of a graded dose response curve therefore represents the concentration of a compound at which 50% of its maximal effect is observed. EC50 also represents the plasma concentration required for obtaining 50% of a maximum effect in vivo. Similarly, the "EC90" refers to the concentration of an agent or composition at which 90% of its maximal effect is observed. The "EC90" can be calculated from the "EC50" and the Hill slope, or it can be determined from the data directly, using routine knowledge in the art. In some embodiments, the EC50 of an agent is less than about 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100, 200 or 500 riM. In some embodiments, a biotherapeutic composition will have an EC50 value of about lnM or less. "Homology" refers to the percentage number of amino acids that are identical or constitute conservative substitutions. Homology may be determined using sequence comparison programs such as GAP (Deveraux et al., 1984, Nucleic Acids Research 12, 387-395). In this way sequences of a similar or substantially different length to those cited herein could be compared by insertion of gaps into the alignment, such gaps being determined, for example, by the comparison algorithm used by GAP.

The term "innate immune response" refers to the responses of immune cells (including macrophages, and natural killer cells (NK)) and the associated mechanisms of modulating cytokine expression and release (e.g., interferons and interferon-signaling), inducing cell death, and inhibiting protein synthesis, which defend the host from infection by pathogens.

By "isolated" is meant material that is substantially or essentially free from components that normally accompany it in its native state. For example, an "isolated polynucleotide," "isolated oligonucleotide," or "isolated oligonucleotide" as used herein, may refer to a polynucleotide that has been purified or removed from the sequences that flank it in a naturally -occurring state, e.g., a DNA fragment that is removed from the sequences that are adjacent to the fragment in the genome. The term "isolating" as it relates to cells refers to the purification of cells (e.g., fibroblasts, lymphoblasts) from a source subject (e.g., a subject with a polynucleotide repeat disease). In the context of mRNA or protein, "isolating" refers to the recovery of mRNA or protein from a source, e.g., cells.

The terms "modulate" includes to "increase" or "decrease" one or more quantifiable parameters, optionally by a defined and/or statistically significant amount. By "increase" or

"increasing," "enhance" or "enhancing," or "stimulate" or "stimulating," refers generally to the ability of one or more agents or compositions to produce or cause a greater physiological response (i.e., downstream effects) in a cell or a subject relative to the response caused by either no agent/compound or a control compound. Relevant physiological or cellular responses (in vivo or in vitro) will be apparent to persons skilled in the art, and may include increases in skeletal muscle mass in a tissue or subject in need thereof. An "increased" or "enhanced" amount is typically a "statistically significant" amount, and may include an increase that is 1.1, 1.2, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50 or more times (e.g., 500, 1000 times), including all integers and decimal points in between and above 1 (e.g., 1.5, 1.6, 1.7. 1.8), the amount produced by no agent/compound (the absence of an agent) or a control compound. The term "reduce" or "inhibit" may relate generally to the ability of one or more agents or compositions to "decrease" a relevant physiological or cellular response, such as expression of a target gene or a symptom of a disease or condition described herein, as measured according to routine techniques in the diagnostic art. Relevant physiological or cellular responses (in vivo or in vitro) will be apparent to persons skilled in the art, and may include reductions or improvements in the symptoms or pathology of lung inflammation or an ILD, as described herein. A "decrease" in a response may be "statistically significant" as compared to the response produced by no agent or composition or a control agent or composition, and may include a 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% decrease, including all integers in between.

In certain embodiments, the "purity" of any given agent in a composition may be specifically defined. For instance, certain compositions may comprise an agent that is at least 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% pure, including all decimals in between, as measured, for example and by no means limiting, by high performance liquid chromatography (HPLC), a well-known form of column chromatography used frequently in biochemistry and analytical chemistry to separate, identify, and quantify compounds.

A "lipid nanoparticle" or "solid lipid nanoparticle" refers to one or more spherical nanoparticles with an average diameter of between about 10 to about 1000 nanometers, and which comprise a solid lipid core matrix that can solubilize lipophilic molecules. The lipid core is stabilized by surfactants (e.g., emulsifiers), and can comprise one or more of triglycerides (e.g., tristearin), diglycerides (e.g., glycerol bahenate), monoglycerides (e.g., glycerol monostearate), fatty acids (e.g., stearic acid), steroids (e.g., cholesterol), and waxes (e.g., cetyl palmitate), including combinations thereof. Lipid nanoparticles are described, for example, in Petrilli et al., Curr Pharm Biotechnol. 15:847-55, 2014; and U.S. Patent Nos. 6,217,912; 6,881,421; 7,402,573; 7,404,969; 7,550,441; 7,727,969; 8,003,621; 8,691,750; 8,871,509; 9,017,726; 9,173,853; 9,220,779; 9,227,917; and 9,278,130, which are incorporated by reference in their entireties.

As used herein, "nucleobase" (Nu), "base pairing moiety" or "base" are used interchangeably to refer to a purine or pyrimidine base found in native DNA or RNA (uracil, thymine, adenine, cytosine, and guanine), as well as analogs of the naturally occurring purines and pyrimidines, that confer improved properties, such as binding affinity to the oligonucleotide. Exemplary analogs include hypoxanthine (the base component of the nucleoside inosine); 2, 6-diaminopurine; 5-methyl cytosine; C5-propynyl-modifed pyrimidines; 9-(aminoethoxy)phenoxazine (G-clamp) and the like.

Further examples of base pairing moieties include, but are not limited to, uracil, thymine, adenine, cytosine, guanine and hypoxanthine having their respective amino groups protected by acyl protecting groups, 2-fluorouracil, 2-fluorocytosine, 5-bromouracil, 5-iodouracil, 2,6-diaminopurine, azacytosine, pyrimidine analogs such as pseudoisocytosine and pseudouracil and other modified nucleobases such as 8-substituted purines, xanthine, or hypoxanthine (the latter two being the natural degradation products). The modified nucleobases disclosed in Chiu and Rana, RNA, 2003, 9, 1034- 1048, Limbach et al. Nucleic Acids Research, 1994, 22, 2183-2196 and Revankar and Rao, Comprehensive Natural Products Chemistry, vol. 7, 313, are also contemplated.

Further examples of base pairing moieties include, but are not limited to, expanded-size nucleobases in which one or more benzene rings has been added. Nucleic base replacements described in the Glen Research catalog (www.glenresearch.com); Krueger AT et al, Acc. Chem. Res., 2007, 40, 141-150; Kool, ET, Acc. Chem. Res., 2002, 35, 936-943; Benner S.A., et al., Nat. Rev. Genet., 2005, 6, 553-543; Romesberg, F.E., et al., Curr. Opin. Chem. Biol., 2003, 7, 723-733; Hirao, I., Curr. Opin. Chem. Biol., 2006, 10, 622-627, are contemplated as useful for the synthesis of the oligonucleotides described herein. Examples of expanded-size nucleobases are shown below:

A nucleobase covalently linked to a ribose, sugar analog or morpholino comprises a nucleoside. "Nucleotides" are composed of a nucleoside together with one phosphate group. The phosphate groups covalently link adjacent nucleotides to one another to form an oligonucleotide.

The terms "polypeptide" and "protein" are used interchangeably herein to refer to a polymer of amino acid residues and to variants and synthetic analogues of the same. Thus, these terms apply to amino acid polymers in which one or more amino acid residues are synthetic non-naturally occurring amino acids, such as a chemical analogue of a corresponding naturally occurring amino acid, as well as to naturally -occurring amino acid polymers.

The term "polynucleotide" and "nucleic acid" includes mRNA, RNA, cRNA, cDNA, and DNA. The term typically refers to polymeric form of nucleotides of at least 10 bases in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide. The term includes single and double stranded forms of DNA. The terms "isolated DNA" and "isolated polynucleotide" and "isolated nucleic acid" refer to a molecule that has been isolated free of total genomic DNA of a particular species. Therefore, an isolated DNA segment encoding a polypeptide refers to a DNA segment that contains one or more coding sequences yet is substantially isolated away from, or purified free from, total genomic DNA of the species from which the DNA segment is obtained. Also included are non-coding polynucleotides (e.g., primers, probes, oligonucleotides), which do not encode a polypeptide. Also included are recombinant vectors, including, for example, expression vectors, viral vectors, plasmids, cosmids, phagemids, phage, viruses, and the like.

Additional coding or non-coding sequences may, but need not, be present within a polynucleotide described herein, and a polynucleotide may, but need not, be linked to other molecules and/or support materials. Hence, a polynucleotide or expressible polynucleotides, regardless of the length of the coding sequence itself, may be combined with other sequences, for example, expression control sequences.

"Expression control sequences" include regulatory sequences of nucleic acids, or the corresponding amino acids, such as promoters, leaders, enhancers, introns, recognition motifs for RNA, or DNA binding proteins, polyadenylation signals, terminators, internal ribosome entry sites (IRES), secretion signals, subcellular localization signals, and the like, which have the ability to affect the transcription or translation, or subcellular, or cellular location of a coding sequence in a host cell. Exemplary expression control sequences are described in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990).

A "promoter" is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3' direction) coding sequence. As used herein, the promoter sequence is bounded at its 3' terminus by the transcription initiation site and extends upstream (5' direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. A transcription initiation site (conveniently defined by mapping with nuclease SI) can be found within a promoter sequence, as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase. Eukaryotic promoters can often, but not always, contain "TATA" boxes and "CAT" boxes. Prokaryotic promoters contain Shine- Dalgarno sequences in addition to the -10 and -35 consensus sequences.

A large number of promoters, including constitutive, inducible and repressible promoters, from a variety of different sources are well known in the art. Representative sources include for example, viral, mammalian, insect, plant, yeast, and bacterial cell types), and suitable promoters from these sources are readily available, or can be made synthetically, based on sequences publicly available on line or, for example, from depositories such as the ATCC as well as other commercial or individual sources. Promoters can be unidirectional (i.e., initiate transcription in one direction) or bidirectional (i.e., initiate transcription in either a 3' or 5' direction). Non-limiting examples of promoters include, for example, the T7 bacterial expression system, pBAD (araA) bacterial expression system, the cytomegalovirus (CMV) promoter, the SV40 promoter, the RSV promoter. Inducible promoters include the Tet system, (US Patents 5,464,758 and 5,814,618), the Ecdysone inducible system (No et al., Proc. Natl. Acad. Sci. (1996) 93 (8): 3346-3351; the T-RExTM system (Invitrogen Carlsbad, CA), LacSwitch® (Stratagene, (San Diego, CA) and the Cre-ERT tamoxifen inducible recombinase system (Indra et al. Nuc. Acid. Res. (1999) 27 (22): 4324-4327; Nuc. Acid. Res. (2000) 28 (23): e99; US Patent No. 7,112,715; and Kramer & Fussenegger, Methods Mol. Biol. (2005) 308: 123-144) or any promoter known in the art suitable for expression in the desired cells.

An "expressible polynucleotide" includes a cDNA, RNA, mRNA or other polynucleotide that comprises at least one coding sequence and optionally at least one expression control sequence, for example, a transcriptional and/or translational regulatory element, and which can express an encoded polypeptide (for example, an HRS polypeptide) upon introduction into a cell, for example, a cell in a subject.

In some embodiments, the expressible polynucleotide is a modified RNA or modified mRNA polynucleotide, for example, a non-naturally occurring RNA analog. In certain embodiments, the modified RNA or mRNA polypeptide comprises one or more modified or non-natural bases, for example, a nucleotide base other than adenine (A), guanine (G), cytosine (C), thymine (T), and/or uracil (U). In some embodiments, the modified mRNA comprises one or more modified or non- natural internucleotide linkages. Expressible RNA polynucleotides for delivering an encoded therapeutic polypeptide are described, for example, in Kormann et al., Nat Biotechnol. 29: 154-7, 2011; and U.S. Application Nos. 2015/0111248; 2014/0243399; 2014/0147454; and 2013/0245104, which are incorporated by reference in their entireties.

In some embodiments, various viral vectors that can be utilized to deliver an expressible polynucleotide include adenoviral vectors, herpes virus vectors, vaccinia virus vectors, adeno- associated virus (AAV) vectors, and retroviral vectors. In some instances, the retroviral vector is a derivative of a murine or avian retrovirus, or is a lentiviral vector. Examples of retroviral vectors in which a single foreign gene can be inserted include, but are not limited to: Moloney murine leukemia virus (MoMuLV), Harvey murine sarcoma virus (HaMuSV), murine mammary tumor virus (MuMTV), SIV, BIV, HIV and Rous Sarcoma Virus (RSV). A number of additional retroviral vectors can incorporate multiple genes. All of these vectors can transfer or incorporate a gene for a selectable marker so that transduced cells can be identified and generated. By inserting a polypeptide sequence of interest into the viral vector, along with another gene that encodes the ligand for a receptor on a specific target cell, for example, the vector may be made target specific. Retroviral vectors can be made target specific by inserting, for example, a polynucleotide encoding a protein. Illustrative targeting may be accomplished by using an antibody to target the retroviral vector. Those of skill in the art will know of, or can readily ascertain without undue experimentation, specific polynucleotide sequences which can be inserted into the retroviral genome to allow target specific delivery of the retroviral vector.

In certain instances, the expressible polynucleotides described herein are engineered for localization within a cell, potentially within a specific compartment such as the nucleus, or are engineered for secretion from the cell or translocation to the plasma membrane of the cell. In exemplary embodiments, the expressible polynucleotides are engineered for nuclear localization.

Also included are biologically active "variants" and "fragments" of the polypeptides described herein, and the polynucleotides that encode the same. "Variants" contain one or more substitutions, additions, deletions, and/or insertions relative to a reference polypeptide or polynucleotide (see, e.g., the Tables and the Sequence Listing). A variant polypeptide or polynucleotide comprises an amino acid or polynucleotide sequence with at least about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% , 99% or more sequence identity or similarity or homology to a reference sequence, as described herein, and substantially retains the activity of that reference sequence. Also included are sequences that consist of or differ from a reference sequences by the addition, deletion, insertion, or substitution of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60,70, 80, 90, 100, 110, 120, 130, 140, 150 or more amino acids or nucleotides and which substantially retain the activity of that reference sequence. In certain embodiments, the additions or deletions include C-terminal and/or N- terminal additions and/or deletions.

The terms "sequence identity" or, for example, comprising a "sequence 50% identical to," as used herein, refer to the extent that sequences are identical on a nucleotide-by -nucleotide basis or an amino acid-by -amino acid basis over a window of comparison. Thus, a "percentage of sequence identity" may be calculated by comparing two optimally aligned sequences over the window of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, I) or the identical amino acid residue (e.g., Ala, Pro, Ser, Thr, Gly, Val, Leu, He, Phe, Tyr, Trp, Lys, Arg, His, Asp, Glu, Asn, Gin, Cys and Met) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. Optimal alignment of sequences for aligning a comparison window may be conducted by computerized implementations of algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package Release 7.0, Genetics Computer Group, 575 Science Drive Madison, Wis., USA) or by inspection and the best alignment (i.e., resulting in the highest percentage homology over the comparison window) generated by any of the various methods selected. Reference also may be made to the BLAST family of programs as for example disclosed by Altschul et al., Nucl. Acids Res. 25:3389, 1997.

By "statistically significant", it is meant that the result was unlikely to have occurred by chance. Statistical significance can be determined by any method known in the art. Commonly used measures of significance include the p-value, which is the frequency or probability with which the observed event would occur, if the null hypothesis were true. If the obtained p-value is smaller than the significance level, then the null hypothesis is rejected. In simple cases, the significance level is defined at a p-value of 0.05 or less.

The term "solubility" refers to the property of an agent provided herein to dissolve in a liquid solvent and form a homogeneous solution. Solubility is typically expressed as a concentration, either by mass of solute per unit volume of solvent (g of solute per kg of solvent, g per dL (100 mL), mg/ml, etc.), molarity, molality, mole fraction or other similar descriptions of concentration. The maximum equilibrium amount of solute that can dissolve per amount of solvent is the solubility of that solute in that solvent under the specified conditions, including temperature, pressure, pH, and the nature of the solvent. In certain embodiments, solubility is measured at physiological pH, or other pH, for example, at pH 5.0, pH 6.0, pH 7.0, or pH 7.4. In certain embodiments, solubility is measured in water or a physiological buffer such as PBS or NaCl (with or without NaP). In specific embodiments, solubility is measured at relatively lower pH (e.g., pH 6.0) and relatively higher salt (e.g., 500mM NaCl and lOmM NaP). In certain embodiments, solubility is measured in a biological fluid (solvent) such as blood or serum. In certain embodiments, the temperature can be about room temperature (e.g., about 20, 21, 22, 23, 24, 25°C) or about body temperature (37°C). In certain embodiments, an agent has a solubility of at least about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 40, 50, 60, 70, 80, 90 or 100 mg/ml at room temperature or at 37°C.

A "subject" or a "subject in need thereof includes a mammalian subject such as a human subject.

"Substantially" or "essentially" means nearly totally or completely, for instance, 95% or greater of some given quantity.

"Therapeutic response" refers to improvement of symptoms (whether or not sustained) based on the administration of the therapeutic response.

As used herein, the term "target" refers to a RNA region, and specifically, to a RNA region of a target gene described herein. The target can include coding and non-coding sequences, 5' upstream sequences, 3 ' downstream sequences, and other RNA sequences described herein.

The term "target sequence" refers to a portion of the target RNA against which the antisense or RNAi agent is directed, for example, the sequence to which the antisense oligonucleotide will hybridize by Watson-Crick base pairing of a complementary sequence, or the sequence that corresponds to the sense strand of the RNAi agent.

As used herein, the term "quantifying", "quantification" or other related words refer to determining the quantity, mass, or concentration in a unit volume, of a nucleic acid, polynucleotide, oligonucleotide, peptide, polypeptide, or protein.

As used herein, the terms "therapeutically effective amount", "therapeutic dose,"

"prophylactically effective amount," or "diagnostically effective amount" is the amount of an agent needed to elicit the desired biological response following administration. Similarly the term "antisense therapy" or "RNAi therapy" includes a therapy that maintains the average steady state concentration of an antisense or RNAi agent in the patient's plasma or other tissue compartment (e.g., muscle tissue) above the minimum effective therapeutic level.

As used herein, "treatment" of a subject (e.g. a mammal, such as a human) or a cell is any type of intervention used in an attempt to alter the natural course of the individual or cell. Treatment includes, but is not limited to, administration of a pharmaceutical composition, and may be performed either prophylactically or subsequent to the initiation of a pathologic event or contact with an etiologic agent. Also included are "prophylactic" treatments, which can be directed to reducing the rate of progression of the disease or condition being treated, delaying the onset of that disease or condition, or reducing the severity of its onset. "Treatment" or "prophylaxis" does not necessarily indicate complete eradication, cure, or prevention of the disease or condition, or associated symptoms thereof.

The term "wild-type" refers to a gene or gene product (e.g., a polypeptide) that is most frequently observed in a population and is thus arbitrarily designed the "normal" or "wild-type" form of the gene.

Histidyl-tRNA Synthetase (HRS) Polypeptides and Polynucleotides

Certain embodiments include histidyl-tRNA synthetase polypeptides ("HRS" or "HisRS" polypeptides), including conjugates (e.g., Fc conjugates), variants, and fragments thereof, and expressible polynucleotides that encode the HRS polypeptides. Histidyl-tRNA synthetases belong to the class II tRNA synthetase family, which has three highly conserved sequence motifs. Class I and II tRNA synthetases are widely recognized as being responsible for the specific attachment of an amino acid to its cognate tRNA in a two-step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. The full-length histidyl-tRNA synthetases typically exist either as a cytosolic homodimer, or an alternatively spliced mitochondrial form.

Certain biological fragments or alternatively spliced isoforms of eukaryotic histidyl-tRNA synthetases, or in some contexts the intact full-length synthetase, modulate certain therapeutically relevant cell-signaling pathways and/or have anti-inflammatory properties. These activities, which are distinct from the classical role of tRNA synthetases in protein synthesis, are referred to herein as "non-canonical activities." For example, as provided herein, HRS polypeptides such as the N-terminal fragment of histidyl-tRNA synthetase (e.g., HRS 1-48, HRS 1-60) are capable, inter alia, of exerting an anti-inflammatory signal by blocking the migration, activation, or differentiation of inflammatory cells (e.g., monocytes, macrophages, T cells, B cells, NK cells, dendritic cells) associated with the sites of active inflammation in vivo. In addition, certain mutations or deletions (e.g., HRS 1-506, HRS 1-60) relative to the full-length HRS polypeptide sequence confer increased activities and/or improved pharmacological properties. The sequences of certain exemplary HRS polypeptides are provided in Table HI below.

KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE

EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQPLCIC

HRS (1-500) 1-500 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 2

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKR

HRS (1-501) 1-501 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 3

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRR

HRS (1-502) 1-502 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 4

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRT

HRS (1-503) 1-503 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 5

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTG

HRS (1-504) 1-504 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 6

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI

YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQ

HRS (1-505) 1-505 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 7

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQP

HisRSl ^N8 1-506 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 8 HRS (1-506) LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQPL

HRS (2-506) 2-506 AEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLL 9

KLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVF DVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIY DLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAEC LKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDS KFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRI GDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLL FEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQT PAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVG LSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQKK LLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEE AGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLV EEIKRRTGQPL

HRS (1-507) 1-507 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 10

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL

LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQPLC

HRS (1-508) 1- 508 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 11

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQPLCI

HisRSl ^N6 1- 48 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 12 HRS (1-48) LK LKAQLGPD

1- 80 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 13

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI

1- 79 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 14

LK

LKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFD

V

1- 78 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 15

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FD

1- 77 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 16

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

F

1- 76 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 17

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

1- 75 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 18

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREK

1- 74 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 19

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVRE

1- 73 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 20

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVR

1- 72 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 21

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAV

1- 71 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 22

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMA

1- 70 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 23

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQM

1- 69 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 24

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQ

1- 68 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 25

LKLKAQLGPDESKQKFVLKTPKGTRDYSPR

1- 67 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 26

LKLKAQLGPDESKQKFVLKTPKGTRDYSP

1- 66 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 27

LKLKAQLGPDESKQKFVLKTPKGTRDYS

1- 65 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 28

LKLKAQLGPDESKQKFVLKTPKGTRDY -64 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 29 LKLKAQLGPDESKQKFVLKTPKGTRD

-63 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 30

LKLKAQLGPDESKQKFVLKTPKGTR

-62 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 31

LKLKAQLGPDESKQKFVLKTPKGT

-61 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 32

LKLKAQLGPDESKQKFVLKTPKG

-60 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 33

LKLKAQLGPDESKQKFVLKTPK

-59 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 34

LKLKAQLGPDESKQKFVLKTP

-58 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 35

LKLKAQLGPDESKQKFVLKT

-57 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 36

LKLKAQLGPDESKQKFVLK

-56 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 37

LKLKAQLGPDESKQKFVL

-55 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 38

LKLKAQLGPDESKQKFV

-54 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 39

LKLKAQLGPDESKQKF

-53 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 40

LKLKAQLGPDESKQK

-52 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 41

LKLKAQLGPDESKQ

-51 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 42

LKLKAQLGPDESK

-50 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 43

LKLKAQLGPDES

-49 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 44

LKLKAQLGPDE

-48 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 45

LKLKAQLGPD

-47 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 46

LKLKAQLGP

-46 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 47

LKLKAQLG

-45 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 48

LKLKAQL

-44 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 49

LKLKAQ

-43 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 50

LK

LKA

-42 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 51

LK

LK

-41 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 52

LK

L

-40 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 53

LK

-80 AERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLL 54

KLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVF DVI

-80 ERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLK 55

LKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFD VI -80 RAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKL 56 KAQLGPDESKQKEVLKTPKGTRDYSPRQMAVREKVFDV

I

-80 AALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLK 57

AQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

-80 ALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKA 58

QLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

-80 LEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQ 59

LGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

-80 EELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQL 60

GPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

-80 ELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLG 61

PDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

0-80 LVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGP 62

DESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

1-80 VKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 63

ESKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

2-80 KLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDE 64

SKQKFVLKTPKGTRDYSPRQMAVREKVFDVI

3-80 LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDES 65

KQKFVLKTPKGTRDYSPRQMAVREKVFDVI

4-80 QGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 66

QKFVLKTPKGTRDYSPRQMAVREKVFDVI

5-80 GERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQ 67

KFVLKTPKGTRDYSPRQMAVREKVFDVI

6-80 RVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKF 68

VLKTPKGTRDYSPRQMAVREKVFDVI

7-80 VRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFV 69

LKTPKGTRDYSPRQMAVREKVFDVI

8-80 RGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVL 70

KTPKGTRDYSPRQMAVREKVFDVI

9-80 GLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLK 71

TPKGTRDYSPRQMAVREKVFDVI

0-80 LKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKT 72

PKGTRDYSPRQMAVREKVFDVI

1-80 KQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTP 73

KGTRDYSPRQMAVREKVFDVI

2-80 QQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK 74

GTRDYSPRQMAVREKVFDVI

3-80 QKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKG 75

TRDYSPRQMAVREKVFDVI

4-80 KASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGT 76

RDYSPRQMAVREKVFDVI

5-80 ASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTR 77

DYSPRQMAVREKVFDVI

6-80 SAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRD 78

YSPRQMAVREKVFDVI

7-80 AELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDY 79

SPRQMAVREKVFDVI

8-80 ELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYS 80

PRQMAVREKVFDVI

9-80 LIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSP 81

RQMAVREKVFDVI

0-80 IEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPR 82

QMAVREKVFDVI

1-80 EEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQ 83

MAVREKVFDVI

2-80 EEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQM 84

AVREKVFDVI

3-80 EVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMA 85 VREKVFDVI

34-80 VAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAV 86

REKVFDVI

35-80 AKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVR 87

EKVFDVI

36-80 KLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVRE 88

KVFDVI

37-80 LLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREK 89

VFDVI

38-80 LK 90

LKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFD VI

39-80 K 91

LKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFD VI

40-80 LKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFD 92

VI

HisRSl ^N1 1-141 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 93

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAM

HisRSl ^N2 1-408 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 94

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLS I GVERI FS IVEQRLEALEEKI RTTE

Hi s RS 1 ^N3 1-113 MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 95

LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKL

Hi s RS 1 ^N4 1-60 MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 96

LKLKAQLGPDESKQKFVLKTPK

Hi s RS 1 ^N5 1-243 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 97

213.3. LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVGYPWWNSCSRI LNYPKTSRPWR AWET

HisRSl ^cl 405-509 RTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLY 98

KKNPKLLNQLQYCEEAGI PLVAI I GEQELKDGVIKLRS VTSREEVDVRREDLVEEIKRRTGQPLCIC

HisRSl ⁰² 1-60 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 99

175-509 LKLKAQLGPDESKQKFVLKTPKDFDIAGNFDPMIPDAE

CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ TPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLS I GVERI FS IVEQRLEALEEKI RTTETQVLVASAQK KLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAI I GEQELKDGVIKLRSVTSREEVDVRREDL VEEIKRRTGQPLCIC HisRSl ⁰³ 1-60 + MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 100 211-509 LKLKAQLGPDESKQKFVLKTPK DRRILDGMFAICGV

SDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVA DRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDL KLLFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVL LQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVP CVGLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASA QKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQY CEEAGIPLVAI IGEQELKDGVIKLRSVTSREEVDVRRE DLVEEIKRRTGQPLCIC

HisRSl ^c4 1-100 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 101

211-509 LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKVNDRRILDGMFAIC GVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPE VADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLG DLKLLFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEA VLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRK VPCVGLSIGVERI FSIVEQRLEALEEKIRTTETQVLVA SAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQL QYCEEAGI PLVAI IGEQELKDGVIKLRSVTSREEVDVR REDLVEEIKRRTGQPLCIC

HisRSl" 1-174 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 102

211-509 LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCVNDRRILDGMFAICGV SDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVA DRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDL KLLFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVL LQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVP CVGLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASA QKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQY CEEAGIPLVAI IGEQELKDGVIKLRSVTSREEVDVRRE DLVEEIKRRTGQPLCIC

HisRSl ⁰⁶ 1-60 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 103

101-509 LKLKAQLGPDESKQKFVLKTPKETLMGKYGEDSKLIYD

LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAK VYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAECL KIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSK FRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIG DYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTP AQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGL SIGVERI FSIVEQRLEALEEKIRTTETQVLVASAQKKL LEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEA GI PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVE EIKRRTGQPLCIC

HisRSl ⁰⁷ Pl-100 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 104

175-509 LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKDFDIAGNFDPMI PD AECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGV SDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVA DRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDL KLLFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVL LQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVP CVGLSIGVERI FSIVEQRLEALEEKIRTTETQVLVASA QKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQY CEEAGIPLVAI IGEQELKDGVIKLRSVTSREEVDVRRE DLVEEIKRRTGQPLCIC

HisRSl ^c8 1-60 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 105

399-509 LKLKAQLGPDESKQKFVLKTPKALEEKIRTTETQVLVA SAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQL

QYCEEAGI PLVAI I GEQELKDGVI KLRSVTSREEVDVR REDLVEEIKRRTGQPLCIC

HisRSl ^c9 1-100 + MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKL 106

399-509 LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVIIRCFKRHGAEVI DTPVFELKALEEKIRTTETQVL VASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLN QLQYCEEAGI PLVAI I GEQELKDGVIKLRSVTSREEVD VRREDLVEEI KRRTGQPLCIC

HisRSl ^cl ° 369-509 MFDPKGRKVPCVGLSIGVERI FS IVEQRLEALEEKI RT 107

TETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKK NPKLLNQLQYCEEAGI PLVAI I GEQELKDGVI KLRSVT SREEVDVRREDLVEEI KRRTGQPLCIC

HisRSl ¹¹ 191-333 CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD 108

SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTG

FL mito. 1-506 MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQVA 109 wild type EAVLTSQLKAHQEKPNFI IKTPKGTRDLSPQHMWREK

ILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGL MYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYH VGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMI PDA ECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVP ESKFRAI CS S I DKLDKMAWKDVRHEMWKKGLAPEVAD RIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLK LLFEYLTLFGIADKI SFDLSLARGLDYYTGVIYEAVLL QTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPC VGLS I GVERI FYIVEQRMKTKGEKVRTTETQVFVATPQ KNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYC ESTGI PLWI IGEQELKEGVIKIRSVASREEVAIKREN FVAEIQKRLSES

Mus musculus FL MADRAALEELVRLQGAHVRGLKEQKASAEQIEEEVTKL 110

LKLKAQLGQDEGKQKFVLKTPKGTRDYSPRQMAVREKV FDVI IRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMI PDAE CLKIMCEILSSLQIGNFLVKVNDRRILDGMFAVCGVPD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQAVEGLGDLKL LFEYLILFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ MPTQAGEEPLGVGSIAAGGRYDGLVGMFDPKGRKVPCV GLS I GVERI FS IVEQRLEAS

EEKVRTTETQVLVASAQKKLLEERLKLVSELWDAGIKA ELLYKKNPKLLNQLQYWEEAGI PLVAI I GEQELRDGVI KLRSVASREEVDVRREDLVEEIRRRTNQPLSTC

Canis lupus FL MAEPAALEELVRLQGERVRGLKQQKASAEQIEEEVAKL 111 familiaris LKLKAQLGPDEGKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI I SCFKRHGAEVIDTPVFELKETLTGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMI PDAE CLEIMCEILR

SLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICSSV DKLDKVSWEEVKNEMVGEKGLAPEVADHIGDYVQQHGG I SLVEQLLQDPELSQNKQALEGLGDLKLLFEYLTLFGI ADKI SFDLSLARGLDYYTGVIYEAVLLQTPVQAGEEPL GVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIF SIVEQRLEATEEKVRTTETQVLVASAQKKLLEERLKLV SELWNAGI KAELLYKKNPKLLNQLQYCEEAGI PLVAI I GEQELKDGVI KLRSVASREEVDVPREDLVEEIKRRTSQ PFCIC Bos taurus FL MADRAALEDLVRVQGERVRGLKQQKASAEQIEEEVAKL 112 LKLKAQLGPDEGKPKFVLKTPKGTRDYSPRQMAVREKV FDVI I SCFKRHGAEVIDTPVFELKETLTGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMLPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKL LFEYLTLFGIADKI SFDLSLARGLDYYTGVIYEAVLLQ PPAPAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQRLEALEEKVRTTETQVLVASAQK KLLEERLKLI SELWDAGIKAELLYKKNPKLLNQLQYCE ETGI PLVAI IGEQELKDGVIKLRSVASREEVDVR REDLVEEIKR RTSQPLCIC

Rattus FL MADPAALEELVRLQGAHVRGLKEQKASAEQIEEEVTKL 113 norvegicus LKLKAQLGHDEGKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLI YDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAE CLKIMCEILSSLQIGNFQVKVNDRRILDGMFAVCGVPD SKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADR IGDYVQQHGGVSLVEQLLQDPKLSQNKQAVEGLGDLKL LFEYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQ MPTQAGEEPLGVGSIAAGGRYDGLVGMFDPKGRKVPCV GLSIGVERI FSIVEQKLEASEEKVRTTETQVLVASAQK KLLEERLKLI SELWDAGIKAELLYKKNPKLLNQLQYCE EAGI PLVAIIGEQE LKDGVIKLRSVTSREEVDVR REDLVEEIRR RTSQPLSM

Gallus FL MADEAAVRQQAEWRRLKQDKAEPDEIAKEVAKLLEMK 114 gallus AHLGGDEGKHKFVLKTPKGTRDYGPKQMAIRERVFSAI

IACFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYDLK

DQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVY

RRDNPAMTRGRYREFYQCDFDIAGQFDPMI PDAECLKI

VQEILSDLQLGDFLIKVNDRRILDGMFAVCGVPDSKFR

TICSSVDKLDKMPWEEVRNEMVGEKGLSPEAADRIGEY

VQLHGGMDLIEQLLQDPKLSQNKLVKEGLGDMKLLFEY

LTLFGITGKI SFDLSLARGLDYYTGVIYEAVLLQQNDH

GEESVSVGSVAGGGRYDGLVGMFDPKGR

KVPCVGI SIGIERI FSILEQRVEASEEKIR

TTETQVLVASAQKKLLEERLKLI SELWDAGIKAEVLYK

KNPKLLNQLQYCEDTGI PLVAIVGEQELKDGWKLRW

ATGEEVNIRRESLVEEIRRRTNQL

Danio rerio FL MAALGLVSMRLCAGLMGRRSAVRLHSLRVCSGMTI SQI 115

DEEVARLLQLKAQLGGDEGKHVFVLKTAKGTRDYNPKQ MAIREKVFNI I INCFKRHGAETIDSPVFELKETLTGKY GEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKIT NIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGQYD AMI PDAECLKLVYEILSELDLGDFRIKVNDRRILDGMF AICGVPDEKFRTICSTVDKLDKLAWEEVKKEMVNEKGL SEEVADRIRDYVSMQGGKDLAERLLQDPKLSQSKQACA GITDMKLLFSYLELFQITDKWFDLSLARGLDYYTGVI YEAILTQANPAPASTPAEQNGAEDAGVSVGSVAGGGRY DGLVGMFDPKAGKCPVWGSALALRGSSPSWSRRQSCLQ RRCAPLKLKCLWLQHRRTF

Macaca FL MAERAALEELVKLQGERVRGLKQQKASAELIEEEVGKL 116 fascicularis LKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKV

FDVI IRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLI YDLKDQGGELLSLRHDLTVPFARYLAMNKLTNIKRYHI AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAE CLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSD SKFRTICSSVDKLDKVSWEEVKNEAVLLQTPAQAGEEP LGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERI

FSIVEQ

RLEALEEKVRTTETQVLVASAQKKLLEERLKLVSELWD AGIKAELLYKKNPKLLNQLQYCEEAGI PLVAI IGEQEL KDGVIKLRSVTSREE VRREDLKTGQNGDFNFYYGYF IDYYWQKWPDTTPFSYKALGA

HRS WHEP X _A -L-X _B -Q-G-X-X-V-R-X-L-K-X-X-K-A-X _C -V- 117 consensus X-X-L-L-X-L-K-X _D

Where :

X is any amino acid

X _A is 0-50 amino acids

X _B is about 5-7 amino acids,

preferably 6 amino acids

X _C is about 7-9 amino acids,

preferably 8 amino acids

X _D is 0-50 amino acids

Accordingly, in certain embodiments, the HRS polypeptide comprises, consists, or consists essentially of a mammalian HRS amino acid sequence in Table HI (e.g., SEQ ID NOs: 1-117), or an active variant or fragment thereof. In some embodiments, the HRS polypeptide comprises, consists, or consists essentially of a human HRS amino acid sequence in Table HI (e.g., SEQ ID NOs: 1-109), or an active variant or fragment thereof. In some embodiments, the expressible polynucleotide encodes an HRS polypeptide that comprises consists, or consists essentially of an amino acid sequence in Table HI (e.g., SEQ ID NO: 1-117), for example, a human HRS sequence in Table HI SEQ ID NOs: 1-109), or an active variant or fragment thereof.

As noted herein, a HRS polypeptide may be altered in various ways including amino acid substitutions, deletions, truncations, additions, and insertions. Methods for such manipulations are generally known in the art. For example, amino acid sequence variants of a HRS reference polypeptide can be prepared by mutations in the DNA. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Kunkel (1985, Proc. Natl. Acad. Sci. USA. 82: 488-492), Kunkel et al., (1987, Methods in Enzymol, 154: 367-382), U.S. Pat. No.

4,873,192, Watson, J. D. et al., ("Molecular Biology of the Gene", Fourth Edition,

Benjamin/Cummings, Menlo Park, Calif, 1987) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al., (1978) Atlas of Protein Sequence and Structure (Natl.

Biomed. Res. Found., Washington, D.C.).

Biologically active truncated and/or variant HRS polypeptides may contain conservative amino acid substitutions at various locations along their sequence, relative to a reference HRS amino acid residue. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art, which can be generally sub-classified as follows: Acidic: The residue has a negative charge due to loss of H ion at physiological pH and the residue is attracted by aqueous solution so as to seek the surface positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium at physiological pH. Amino acids having an acidic side chain include glutamic acid and aspartic acid.

Basic: The residue has a positive charge due to association with H ion at physiological pH or within one or two pH units thereof (e.g., histidine) and the residue is attracted by aqueous solution so as to seek the surface positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium at physiological pH. Amino acids having a basic side chain include arginine, lysine and histidine.

Charged: The residues are charged at physiological pH and, therefore, include amino acids having acidic or basic side chains (i.e., glutamic acid, aspartic acid, arginine, lysine and histidine).

Hydrophobic: The residues are not charged at physiological pH and the residue is repelled by aqueous solution so as to seek the inner positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium. Amino acids having a hydrophobic side chain include tyrosine, valine, isoleucine, leucine, methionine, phenylalanine and tryptophan.

Neutral/polar: The residues are not charged at physiological pH, but the residue is not sufficiently repelled by aqueous solutions so that it would seek inner positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium. Amino acids having a neutral/polar side chain include asparagine, glutamine, cysteine, histidine, serine and threonine.

This description also characterizes certain amino acids as "small" since their side chains are not sufficiently large, even if polar groups are lacking, to confer hydrophobicity. With the exception of proline, "small" amino acids are those with four carbons or less when at least one polar group is on the side chain and three carbons or less when not. Amino acids having a small side chain include glycine, serine, alanine and threonine. The gene-encoded secondary amino acid proline is a special case due to its known effects on the secondary conformation of peptide chains. The structure of proline differs from all the other naturally -occurring amino acids in that its side chain is bonded to the nitrogen of the a-amino group, as well as the a-carbon. Several amino acid similarity matrices are known in the art (see e.g., PAM120 matrix and PAM250 matrix as disclosed for example by Dayhoff et al., 1978, A model of evolutionary change in proteins). Matrices for determining distance relationships In M. O. Dayhoff, (ed.), Atlas of protein sequence and structure, Vol. 5, pp. 345-358, National Biomedical Research Foundation, Washington DC; and by Gonnet et al., (Science, 256: 14430-1445, 1992), however, include proline in the same group as glycine, serine, alanine and threonine. Accordingly, proline is classified as a "small" amino acid.

The degree of attraction or repulsion required for classification as polar or nonpolar is arbitrary and, therefore, amino acids specifically contemplated by the invention have been classified as one or the other. Most amino acids not specifically named can be classified on the basis of known behavior. Amino acid residues can be further sub -classified as cyclic or non-cyclic, and aromatic or non-aromatic, self-explanatory classifications with respect to the side-chain substituent groups of the residues, and as small or large. The residue is considered small if it contains a total of four carbon atoms or less, inclusive of the carboxyl carbon, provided an additional polar substituent is present; three or less if not. Small residues are, of course, always non-aromatic. Dependent on their structural properties, amino acid residues may fall in two or more classes. For the naturally -occurring protein amino acids, sub-classification according to this scheme is presented in Table A.

Conservative amino acid substitution also includes groupings based on side chains. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic -hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulphur-containing side chains is cysteine and methionine. For example, it is reasonable to expect that replacement of a leucine with an isoleucine or valine, an aspartate with a glutamate, a threonine with a serine, or a similar replacement of an amino acid with a structurally related amino acid will not have a major effect on the properties of the resulting variant polypeptide. Whether an amino acid change results in a functional truncated and/or variant HRS polypeptide can readily be determined by assaying its non-canonical activity, as described herein. Conservative substitutions are shown in Table B under the heading of exemplary substitutions. Amino acid substitutions falling within the scope of the invention, are, in general, accomplished by selecting substitutions that do not differ significantly in their effect on maintaining (a) the structure of the peptide backbone in the area of the substitution, (b) the charge or hydrophobicity of the molecule at the target site, (c) the bulk of the side chain, or (d) the biological function. After the substitutions are introduced, the variants are screened for biological activity. Table B

Original Residue Exemplary Substitutions Preferred. Substitutions

Al Val, Leu, He Val

Arg Lys, Gin, Asn Lys

Asn Gin, His, Lys, Arg Gin

Asp Glu Glu

Cys Ser, Ala, Leu, Val Ser, Ala

Gin Asn, His, Lys, Asn

Glu Asp, Lys Asp

Gly Pro Pro

His Asn, Gin, Lys, Arg Arg

He Leu, Val, Met, Ala, Phe, Leu

Norleu

Leu Norleu, He, Val, Met, He

Ala, Phe

Lys Arg, Gin, Asn Arg

Met Leu, He, Phe Leu

Phe Leu, Val, He, Ala Leu

Pro Gly Gly

Ser Thr Thr

Thr Ser Ser

Trp Tyr Tyr

Tyr Trp, Phe, Thr, Ser Phe

Val He, Leu, Met, Phe, Ala, Leu

Norleu

Alternatively, similar amino acids for making conservative substitutions can be grouped into three categories based on the identity of the side chains. The first group includes glutamic acid, aspartic acid, arginine, lysine, histidine, which all have charged side chains; the second group includes glycine, serine, threonine, cysteine, tyrosine, glutamine, asparagine; and the third group includes leucine, isoleucine, valine, alanine, proline, phenylalanine, tryptophan, methionine, as described in Zubay, G., Biochemistry, third edition, Wm.C. Brown Publishers (1993).

In some embodiments, HRS polypeptides have one or more cysteine insertions or substitutions, for example, where one or more non-cysteine residues are substituted with a cysteine residue (e.g., to alter stability, to facilitate thiol-based conjugation of an Fc fragment, to facilitate thiol-based attachment of PEG or other molecules). In some embodiments, the one or more cysteine substitutions are near the N-terminus and/or C-terminus of the HRS polypeptide, or other surface exposed regions of a HRS polypeptide. Particular embodiments include where one or more of residues within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids relative to the N-terminus and/or C-terminus of an HRS polypeptide are substituted with a cysteine residue. In some embodiments, cysteine residues may be added to the HRS polypeptide through the creation of N, or C-terminal fusion proteins. Such fusion proteins may be of any length, but will typically be about 1-5, or about 5-10, about 10 to 20, or about 20 to 30 amino acids in length. Specific examples of cysteine modified proteins are shown in Table H2, which are based on the HRS polypeptide HRS(l-60). This approach can be applied to the HRS polypeptides of Table HI and other HRS polypeptides described herein.

Accordingly, in certain embodiments, the HRS polypeptide comprises, consists, or consists essentially of an amino acid sequence in Table H2 (SEQ ID NO: 118-120) or an active variant or fragment thereof. In some embodiments, the expressible polynucleotide encodes an HRS polypeptide that comprises consists, or consists essentially of an amino acid sequence in Table H2 (e.g., SEQ ID NO: 118-120) or an active variant or fragment thereof.

In some embodiments, the HRS polypeptide have mutations in which the endogenous or naturally -occurring cysteine residues are mutated to alternative amino acids, or deleted. In some embodiments, the insertion or substitution of cysteine residue(s) into the HRS polypeptide is combined with the elimination of other surface exposed reactive cysteine residues. Accordingly, in some embodiments, an HRS polypeptide comprises one or more substitutions and/or deletions at any one or more of Cys83, Cysl74, Cysl91, Cysl96, Cys224, Cys235, Cys379, Cys455, Cys507, and/or Cys509 (as defined by SEQ ID NO: 1), for instance, to remove naturally -occurring cysteine residues, including combinations thereof.

Specific embodiments include an HRS polypeptide of Table HI having a mutation or deletion of any one or more of Cys83, Cysl74, Cysl91, Cysl96, Cys224, Cys235, Cys379, Cys455, or the deletion of Cys507 and Cys509, for instance, by the deletion of the C-terminal 3 amino acids (Δ507-509). Exemplary mutations at these positions include for example the mutation of cysteine to serine, alanine, leucine, valine or glycine. In certain embodiments, amino acid residues for specific cysteine substitutions can be selected from naturally -occurring substitutions that are found in HRS orthologs from other species and organisms. Exemplary substitutions of this type are presented in Table H3

In some embodiments, the naturally -occurring cysteines selected for mutagenesis are selected based on their surface exposure. Accordingly, in one aspect the cysteine residues selected for substitution are selected from Cys224, Cys235, Cys507 and Cys509. In some embodiments, the last three (C-terminal) residues of SEQ ID NO: 1 are deleted so as to delete residues 507 to 509. In some embodiments, the cysteines are selected for mutation or deletion so as to eliminate an intramolecular cysteine pair, for example Cysl74 and Cysl91.

Specific examples of cysteine mutations/substitutions (indicated in bold underline) to reduce surface exposed cysteine residues include those listed below in Table H4.

I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

HRS (1-506) MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 123 C191A QKFVLKTPKGTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELKET

LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAEALKIMCEILSSLQI GDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVG EKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSV AAGGRYDGLVGMFDPKGRKVPCVGLSIGVERI FSIVEQRLEALEEKIRTTE TQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAG I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

HRS (1-506) MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 124 C191S QKFVLKTPKGTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELKET

LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAESLKIMCEILSSLQI GDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVG EKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSV AAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTE TQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAG I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

HRS (1-506) MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 125 CI 91V QKFVLKTPKGTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELKET

LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAEVLKIMCEILSSLQI GDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVG EKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSV AAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTE TQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAG I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

HRS (1-506) MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 126 C224S QKFVLKTPKGTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELKET

LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAECLKIMCEILSSLQI GDFLVKVNDRRILDGMFAISGVSDSKFRTICSSVDKLDKVSWEEVKNEMVG EKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSV AAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTE TQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAG I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

HRS (1-506) MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESK 127 C235S QKFVLKTPKGTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELKET

LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIA KVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMI PDAECLKIMCEILSSLQI GDFLVKVNDRRILDGMFAICGVSDSKFRTISSSVDKLDKVSWEEVKNEMVG EKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLF EYLTLFGIDDKI SFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSV AAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTE TQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAG I PLVAI IGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPL

Accordingly, in certain embodiments, the HRS polypeptide comprises, consists, or consists essentially of an amino acid sequence in TableH4 (SEQ ID NO: 121-127) or an active variant or fragment thereof. In some embodiments, the expressible polynucleotide encodes an HRS polypeptide that comprises consists, or consists essentially of an amino acid sequence in TableH4 (e.g., SEQ ID NO: 121-127) or an active variant or fragment thereof. In some embodiments, such cysteine substituted mutants are modified to engineer-in, insert, or otherwise introduce a new surface exposed cysteine residue at a defined surface exposed position, where the introduced residue does not substantially interfere with the non-canonical activity of the HRS polypeptide. Specific examples include for example the insertion (or re-insertion back) of additional cysteine residues at the N- or C-terminus of any of the reduced cysteine HRS polypeptides described above. In some embodiments, the insertion of such N- or C-terminal surface exposed cysteines involves the re-insertion of the last 1, last 2, or last 3 naturally occurring C-terminal amino acids of the full length human HRS to a reduced cysteine variant of a HRS polypeptide e.g., the reinsertion of all or part of the sequence CIC (Cys He Cys). Exemplary reduced cysteine mutants include for example any combination of mutations (or the deletion of) at residues Cys 174, Cysl91, Cys224, and Cys235, and or the deletion or substitution of Cys507 and Cys509 (based on the numbering of full length human cytosolic HRS (SEQ ID NO: 1) in any of the HRS polypeptides of Table HI

For some types of site-specific conjugation or attachment to heterologous molecules such as Fc regions or PEG or other heterologous molecules, HRS polypeptides may have one or more glutamine substitutions, where one or more naturally -occurring (non-glutamine) residues are substituted with glutamine, for example, to facilitate transglutaminase-catalyzed attachment of the molecule(s) to the glutamine 's amide group. In some embodiments, glutamine substitutions are introduced near the N-terminus and/or C-terminus of the HRS polypeptide. Particular embodiments include where one or more of residues within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids relative to the N-terminus and/or C-terminus of an HRS polypeptide are substituted with a glutamine residue. These and related HRS polypeptides can also include substitutions (e.g., conservative substitutions) to remove any naturally -occurring glutamine residues, if desired, and thereby regulate the degree of site-specific conjugation or attachment.

For certain types of site-specific conjugation or attachment to heterologous molecules such as Fc regions or PEG or other heterologous molecules, HRS polypeptides may have one or more lysine substitutions, where one or more naturally -occurring (non-lysine) residues are substituted with lysine, for example, to facilitate acylation or alkylation-based attachment of molecule(s) to the lysine's amino group. These methods also typically result in attachment of molecule(s) to the N-terminal residue. In some embodiments, lysine substations are near the N-terminus and/or C-terminus of the HRS polypeptide. Particular embodiments include where one or more of residues within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids to the N-terminus and/or C-terminus of an HRS polypeptide are substituted with a lysine residue. These and related HRS polypeptides can also include substitutions (e.g., conservative substitutions) to remove any naturally-occurring lysine residues, if desired, and thereby regulate the degree of site-specific conjugation or attachment. Site-specific conjugation to HRS polypeptides may also be performed by substituting one or more solvent accessible surface amino acids of a HRS polypeptide. For example, suitable solvent accessible amino acids may be determined based on the predicted solvent accessibility using the SPIDDER server (http://sppider.cchmc.org/) using the published crystal structure of an exemplary HRS polypeptide (see Xu et al, Structure. 20: 1470-7, 2012; and U.S. Application No. 61/674,639). Based on this analysis several amino acids on the surface may potentially be used as mutation sites to introduce functional groups suitable for conjugation or attachment. The surface accessibility score of amino acids based on the crystal structure can be calculated, where the higher scores represent better accessibility. In particular embodiments, higher scores (for example, >40) are preferred. Accordingly in some embodiments an amino acid position have a surface accessibility score of greater than 40 may be used to introduce a cysteine, lysine, glutamine, or other non-naturally -occurring amino acid.

In particular embodiments, a solvent accessible surface amino acid is selected from the group consisting of: alanine, glycine, and serine, and can be substituted with naturally occurring amino acids including, but not limited to, cysteine, glutamine, or lysine, or a non-naturally occurring amino acid that is optimized for site specific conjugation or attachment.

Certain embodiments include site-specific conjugation or attachment to an HRS polypeptide at any amino acid position by virtue of substituting a non-naturally-occurring amino acid comprising a functional group that will form a covalent bond with the functional group attached to a heterologous molecules such as an Fc region or PEG or other heterologous molecule. Non-natural amino acids can be inserted or substituted at, for example, one or more of residues within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids relative to the N-terminus and/or C-terminus, at the N-terminus and/or C-terminus, or at a solvent accessible surface amino acid residue of an HRS polypeptide described herein.

In particular embodiments, non-naturally occurring amino acids include, without limitation, any amino acid, modified amino acid, or amino acid analogue other than selenocysteine and the following twenty genetically encoded alpha-amino acids: alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine. The generic structure of an alpha-amino acid is illustrated by the following formula:

A non-natural amino acid is typically any structure having the foregoing formula wherein the R group is any substituent other than one used in the twenty natural amino acids. See, e.g., biochemistry texts such as Biochemistry by L. Stryer, 3rd ed. 1988, Freeman and Company, New York, for structures of the twenty natural amino acids. Note that the non-natural amino acids disclosed herein may be naturally occurring compounds other than the twenty alpha-amino acids above.

Because the non-natural amino acids disclosed herein typically differ from the natural amino acids in side chain only, the non-natural amino acids form amide bonds with other amino acids, e.g. , natural or non-natural, in the same manner in which they are formed in naturally occurring proteins. However, the non-natural amino acids have side chain groups that distinguish them from the natural amino acids. For example, R in foregoing formula optionally comprises an alkyl-, aryl-, aryl halide, vinyl halide, alkyl halide, acetyl, ketone, aziridine, nitrile, nitro, halide, acyl-, keto-, azido-, hydroxyl-, hydrazine, cyano-, halo-, hydrazide, alkenyl, alkynyl, ether, thio ether, epoxide, sulfone, boronic acid, boronate ester, borane, phenylboronic acid, thiol, seleno-, sulfonyl-, borate, boronate, phospho, phosphono, phosphine, heterocyclic-, pyridyl, naphthyl, benzophenone, a constrained ring such as a cyclooctyne, thio ester, enone, imine, aldehyde, ester, thioacid, hydroxylamine, amino, carboxylic acid, alpha-keto carboxylic acid, alpha or beta unsaturated acids and amides, glyoxyl amide, or organosilane group, or the like or any combination thereof.

Specific examples of unnatural amino acids include, but are not limited to, p-acetyl-L- phenylalanine, O-methyl-L-tyrosine, an L-3-(2-naphthyl)alanine, a 3-methyl-phenylalanine, an 0-4- allyl-L-tyrosine, a 4-propyl-L-tyrosine, a tri-O-acetyl-GlcNAc -serine, β-0-GlcNAc-L-serine, a tri- O-acety 1-GalNAc-a-threonine, an a-GalNAc-L-threonine, an L-Dopa, a fluorinated phenylalanine, an isopropyl-L-phenylalanine, a p-azido-L-phenylalanine, a p-acyl-L-phenylalanine, a p-benzoyl-L- phenylalanine, an L-phosphoserine, a phosphonoserine, a phosphonotyrosine, a p-iodo-phenylalanine, a p-bromophenylalanine, a p-amino-L-phenylalanine, an isopropyl-L-phenylalanine, those listed below, or elsewhere herein, and the like.

Accordingly, one may select a non-naturally occurring amino acid comprising a functional group that forms a covalent bond with any preferred functional group of a desired molecule (e.g., Fc region, PEG). Non-natural amino acids, once selected, can either be purchased from vendors, or chemically synthesized. Any number of non-natural amino acids may be incorporated into the target molecule and may vary according to the number of desired molecules that are to be attached. The molecules may be attached to all or only some of the non-natural amino acids. Further, the same or different non-natural amino acids may be incorporated into a HRS polypeptide, depending on the desired outcome. In certain embodiments, about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more non-natural amino acids are incorporated into a HRS polypeptide any or all of which may be conjugated to a molecule comprising a desired functional group.

In certain aspects, the use of non-natural amino acids can be utilized to modify (e.g., increase) a selected non-canonical activity of an HRS polypeptide, or to alter the in vivo or in vitro half -life of the protein. Non-natural amino acids can also be used to facilitate (selective) chemical modifications (e.g., pegylation) of an HRS polypeptide, as described herein. For instance, certain non-natural amino acids allow selective attachment of polymers such as an Fc region or PEG to a given protein, and thereby improve their pharmacokinetic properties.

Specific examples of amino acid analogs and mimetics can be found described in, for example, Roberts and Vellaccio, The Peptides: Analysis, Synthesis, Biology, Eds. Gross and Meinhofer, Vol. 5, p. 341, Academic Press, Inc., New York, N.Y. (1983), the entire volume of which is incorporated herein by reference. Other examples include peralkylated amino acids, particularly permethylated amino acids. See, for example, Combinatorial Chemistry, Eds. Wilson and Czarnik, Ch. 11, p. 235, John Wiley & Sons Inc., New York, N.Y. (1997), the entire book of which is incorporated herein by reference. Yet other examples include amino acids whose amide portion (and, therefore, the amide backbone of the resulting peptide) has been replaced, for example, by a sugar ring, steroid, benzodiazepine or carbo cycle. See, for instance, Burger's Medicinal Chemistry and Drug Discovery, Ed. Manfred E. Wolff, Ch. 15, pp. 619-620, John Wiley & Sons Inc., New York, N.Y. (1995), the entire book of which is incorporated herein by reference. Methods for synthesizing peptides, polypeptides, peptidomimetics and proteins are well known in the art (see, for example, U.S. Pat. No. 5,420,109; M. Bodanzsky, Principles of Peptide Synthesis (1st ed. & 2d rev. ed.), Springer- Verlag, New York, N.Y. (1984 & 1993), see Chapter 7; Stewart and Young, Solid Phase Peptide Synthesis, (2d ed.), Pierce Chemical Co., Rockford, 111. (1984), each of which is incorporated herein by reference). Accordingly, the HRS polypeptides can be composed of naturally occurring and non- naturally occurring amino acids as well as amino acid analogs and mimetics.

In certain embodiments, a HRS polypeptide comprises, consists, or consists essentially of the minimal active fragment of a full-length HRS polypeptide capable of modulating an antiinflammatory activity in vivo or having antibody or auto-reactive T-cell blocking activities. In some embodiments, such a minimal active fragment comprises, consists, or consists essentially of the WHEP domain (e.g., about amino acids 1-43 of SEQ ID NO: l) or an active variant or fragment thereof. In some aspects, the minimal active fragment comprises, consists, or consists essentially of the aminoacylation domain, (e.g., about amino acids 54-398 of SEQ ID NO: 1) or an active variant or fragment thereof. In some aspects, the minimal active fragment comprises, consists, or consists essentially of the anticodon binding domain (e.g., about amino acids 406-501 of SEQ ID NO: l) or an active variant or fragment thereof.

In certain embodiments, the HRS polypeptide is about, at least about, and/or up to about 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 501, 502, 503, 504, 505, 506, 507, 508, or 509 amino acids in length, including all integers ranges in between, and comprises, consists, or consists essentially of an amino acid sequence in Table HI, Table H2, or Table H4.

In certain embodiments, the HRS polypeptide possesses at least one non-canonical activity, for example, an anti-inflammatory activity or cross-reactivity with auto-antibody or auto reactive T- cell from a subject with a disease associated with autoantibodies (e.g., Jo-1 antibodies) to a histidyl- tRNA synthetase. Assays to determine anti-inflammatory activity, including routine measurements of cytokine release from in vitro cell based, and animal studies are well established in the art (see, for example, Wittmann et al., J Vis Exp. (65):e4203. doi: 10.3791/4203, 2012; Feldman et al., Mol Cell. 47:585-95, 2012; Clutterbuck et al., J Proteomics. 74:704-15, 2011, Giddings and Maitra, J Biomol Screen. 15: 1204-10, 2010; Wijnhoven et al., Glycoconj J. 25: 177-85, 2008; and Frow et al., Med Res Rev. 24:276-98, 2004) and can be readily used to profile and optimize anti-inflammatory activity. An exemplary in vivo experimental system is also described in the accompanying Examples.

In some embodiments, the HRS polypeptide does not significantly compete for disease associated auto-antibody binding (e.g., Jo-1 antibody) to wild type histidyl-tRNA synthetase in a competitive ELISA up to a concentration of about 1 to 5 x 10-7M, or higher. Accordingly, in some embodiments, the HRS polypeptide has a lower affinity to disease associated auto-antibody than wild type histidyl-tRNA synthetase (SEQ ID NO: 1) as measured in a competitive ELISA. In some embodiments, the HRS polypeptide has an apparent affinity for the disease associated auto-antibody (e.g., Jo-1 antibody) which is at least about 10 fold less, or at least about 20 fold less, or at least about 50 fold less, or at least about 100 fold less than the affinity of the disease associated auto-antibody to wild type human (SEQ ID NO: 1).

It will be appreciated that in any of the HRS polypeptides, the N-terminal acid of the HRS polypeptide (for example, the N-terminal Met) may be deleted.

In other embodiments, fusion proteins of HRS polypeptide to other (non HARS) proteins (e.g. heterologous proteins or polypeptides) are also included, and these fusion proteins may modulate the HRS polypeptide's biological activity, secretion, antigenicity, targeting, biological life, ability to penetrate cellular membranes, or the blood brain barrier, or pharmacokinetic properties. Examples of fusion proteins that improve pharmacokinetic properties ("PK modifiers") include without limitation, fusions to human albumin (Osborn et al.: Eur. J. Pharmacol. 456(1-3): 149-158, (2002)), antibody Fc domains, poly Glu or poly Asp sequences, and transferrin. Additionally, fusion with conformationally disordered polypeptide sequences composed of the amino acids Pro, Ala, and Ser ('PASylation') or hydroxyethyl starch (sold under the trademark HESYLATION®) provides a simple way to increase the hydrodynamic volume of the HRS polypeptide. This additional extension adopts a bulky random structure, which significantly increases the size of the resulting fusion protein. By this means the typically rapid clearance of smaller HRS polypeptides via kidney filtration is retarded by several orders of magnitude. Additionally use of Ig G fusion proteins has also been shown to enable some fusion protein proteins to penetrate the blood brain barrier (Fu et al., (2010) Brain Res. 1352:208-13). Examples of fusion proteins that modulate the antigenicity, or immunomodulatory properties of the HRS polypeptide include fusions to T cell binding ligands, including for example, MHC Class I and II proteins, b-2 microglobulin, portions of LFA-3, portions of the Fc region of the heavy chain, and conjugates and derivatives thereof; Examples of such fusion proteins are described in for example EP 1 964 854, US Patent Nos.5,468,481; 5,130,297; 5,635,363; 6,451,314 and US 2009/0280135.

Additionally in some embodiments, the HRS polypeptide can include synthetic, or naturally occurring secretion signal sequences, derived from other well characterized secreted proteins. In some embodiments such proteins, may be processed by proteolytic cleavage to form the HRS polypeptide in situ. In some embodiments the HRS polypeptide can comprise heterologous proteolytic cleavage sites, to enable the in situ expression, and production of the HRS polypeptide either at an intracellular, or an extracellular location. Other fusions proteins may also include for example fusions of HRS polypeptide to ubiquitin to provide a new N-terminal amino acid, or the use of a secretion signal to mediate high level secretion of the HRS polypeptide into the extracellular medium, or N, or C- terminal epitope tags to improve purification or detection, and fusions to cell penetrating peptides..

In certain aspects, the use of non-natural amino acids can be utilized to modify (e.g., increase) a selected non-canonical activity of a HRS polypeptide, or to alter the in vivo or in vitro half-life of the protein. Non-natural amino acids can also be used to facilitate (selective) chemical modifications (e.g., pegylation) of a HRS protein, as described elsewhere herein. For instance, certain non-natural amino acids allow selective attachment of polymers such as PEG to a given protein, and thereby improve their pharmacokinetic properties.

Certain embodiments include HRS-Fc conjugates, which comprise at least one Fc region that is covalently attached to one or more HRS polypeptides. Examples of HRS-Fc conjugates include fusion proteins and various forms of chemically cross-linked proteins. A wide variety of Fc region sequences may be employed in the HRS-Fc conjugates, including wild-type sequences from any number of species, as well as variants, fragments, hybrids, and chemically modified forms thereof. The HRS-Fc polypeptides may also (optionally) comprise one or more linkers, which typically separate the Fc region(s) from the HRS polypeptide(s), including peptide linkers and chemical linkers, as described herein and known in the art. It will be appreciated that in any of these HRS-Fc conjugates the native N or C terminal amino acid of the HRS polypeptides, or native N or C- amino acid in the Fc domain, may be deleted and/or replaced with non-native amino acid(s), for example, to facilitate expression and or cloning or to serve as a linker sequence between the two proteins.

HRS-Fc conjugate polypeptides can provide a variety of advantages relative to un-conjugated or unmodified HRS polypeptides, e.g., corresponding HRS polypeptides of the same or similar sequence having no Fc region(s) attached thereto. Merely by way of illustration, the covalent attachment of one or more Fc regions can alter (e.g., increase, decrease) the HRS polypeptide's solubility, half-life (e.g., in serum, in a selected tissue, in a test tube under storage conditions, for example, at room temperature or under refrigeration), dimerization or multimerization properties, biological activity or activities, for instance, by providing Fc-region-associated effector functions (e.g., activation of the classical complement cascade, interaction with immune effector cells via the Fc receptor (FcR), compartmentalization of immunoglobulins), cellular uptake, intracellular transport, tissue distribution, and/or bioavailability, relative to an unmodified HRS polypeptide having the same or similar sequence. In certain aspects, Fc regions can confer effector functions relating to complement-dependent cytotoxicity (CDC), antibody -dependent cell-mediated cytotoxicity (ADCC), and/or antibody -dependent cell-mediated phagocytocis (ADCP), which are believed to play a role in clearing specific target cells such as tumor cells and infected cells.

Certain embodiments employ HRS-Fc fusion proteins. "Fusion proteins" are defined elsewhere herein and well known in the art, as are methods of making fusion proteins (see, e.g., U.S. Patent Nos. 5, 116,964; 5,428, 130; 5,455,165; 5,514,582; 6,406,697; 6,291,212; and 6,300,099 for general disclosure and methods related to Fc fusion proteins). In a HRS-Fc fusion protein, the Fc region can be fused to the N-terminus of the HRS polypeptide, the C-terminus, or both. In some embodiments, one or more Fc regions can be fused internally relative to HRS sequences, for instance, by placing an Fc region between a first HRS sequence (e.g., domain) and a second HRS sequence (e.g., domain), where the first HRS sequence is fused to the N-terminus of the Fc region and the second HRS sequence is fused to the C-terminus of the Fc region. In specific embodiments, the first and second HRS sequences are identical. In other embodiments, the first and second HRS sequences are different (e.g., they include different functional domains of the HRS polypeptide). Certain HRS-Fc fusion proteins can also include additional heterologous protein sequences, that is, non-Fc region and non-HRS polypeptide sequences.

The term "HRS-Fc" can indicate, but does not necessarily indicate, the N-terminal or C- terminal attachment of the Fc region to the HRS polypeptide. For instance, in certain instances the term "Fc-HRS" indicates fusion of the Fc region to the N-terminus of the HRS polypeptide, and the term "HRS-Fc" indicates fusion of the Fc region to the C-terminus of the HRS polypeptide. However, either term can be used more generally to refer to any fusion protein or conjugate of an Fc region and a HRS polypeptide.

In some embodiments the HRS-Fc fusion proteins may comprise tandemly repeated copies of the HRS polypeptide coupled to a single Fc domain, optionally separated by linker peptides.

Exemplary tandemly repeated HRS-Fc fusion proteins are provided in Table H5. The preparation and sequences for specific tandemly repeated HRS-Fc conjugates are illustrated in the Examples.

Table H5. Exemplary Tandem HRS-Fc conjugates

HRS polypeptide-L-HRS-polypeptide-L-Fc

HRS-polypeptide-L-HRS-polypeptide-L-HRS-polypeptide-L-Fc

HRS-polypeptide-L-HRS-polypeptide-L-HRS-polypeptide-L-HRS-po lypeptide- L-

Fc

Fc-L-HRS-polypeptide-L-HRS-polypeptide

Fc-L-HRS-po1ypeptide-L-HRS-L-HRS-polypeptide

Fc-L-HRS-polypeptide-L-HRS-L-HRS-L-HRS-polypeptide

Where : "Fc" is an Fc domain as described herein

"HRS-polypeptide" is an HRS polypeptide as described herein

"L" is an optional peptide linker

Certain embodiments relate to HRS-Fc conjugates, where, for instance, one or more Fc regions are chemically conjugated or cross-linked to the HRS polypeptide(s). In these and related aspects, the Fc region can be conjugated to the HRS polypeptide at the N-terminal region (e.g., within the first 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or so amino acids), the internal region (between the N- terminal and C-terminal regions), and/or the C-terminal region (e.g., within the last 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or so amino acids). Polypeptides can be conjugated or cross-linked to other polypeptides according to a variety of routine techniques in the art. For instance, certain techniques employ the carboxyl-reactive carbodiimide crosslinker EDC (or ED AC), which covalently attaches via D, E, and C-terminal carboxyl groups. Other techniques employ activated EDC, which covalently attaches via K and N-terminal amino groups). Still other techniques employ /w-maleimidobenzoyl-jV- hydoxysuccinimide ester (MBS) or Sulfo-MBS, which covalently attach via the thiol group of a cysteine residue (see also U.S. Application No. 2007/0092940 for cysteine engineered Ig regions that can be used for thiol conjugation). Such cross-linked proteins can also comprise linkers, including cleavable or otherwise releasable linkers (e.g., enzymatically cleavable linkers, hydrolysable linkers), and non-cleavable linkers (i.e., physiologically-stable linkers). Certain embodiments may employ non-peptide polymers (e.g., PEG polymers; HRS-N-PEG-N-Fc conjugate) as a cross-linker between the Fc region(s) and the HRS polypeptide(s), as described, for example, in U.S. Application No. 2006/0269553. See also US Application No. 2007/0269369 for exemplary descriptions of Fc region conjugation sites.

In certain embodiments, discussed in greater detail below, variant or otherwise modified Fc regions can be employed, including those having altered properties or biological activities relative to wild-type Fc region(s). Examples of modified Fc regions include those having mutated sequences, for instance, by substitution, insertion, deletion, or truncation of one or more amino acids relative to a wild-type sequence, hybrid Fc polypeptides composed of domains from different immunoglobulin classes/subclasses, Fc polypeptides having altered glycosylation/sialylation patterns, and Fc polypeptides that are modified or derivatized, for example, by biotinylation (see, e.g., US Application No. 2010/0209424), phosphorylation, sulfation, etc., or any combination of the foregoing. Such modifications can be employed to alter (e.g., increase, decrease) the binding properties of the Fc region to one or more particular FcRs (e.g., FcyRI, FcyRIIa, FcyRIIb, FcyRIIc, FcyRIIIa, FcyRIIIb, FcRn), its pharmacokinetic properties (e.g., stability or half-life, bioavailability, tissue distribution, volume of distribution, concentration, elimination rate constant, elimination rate, area under the curve (AUC), clearance, C _ma x, , Cmin, fluctuation), its immunogenicity, its complement fixation or activation, and/or the CDC/ADCC/ADCP-related activities of the Fc region, among other properties described herein, relative to a corresponding wild-type Fc sequence. The "Fc region" of a HRS-Fc conjugate provided herein is usually derived from the heavy chain of an immunoglobulin (Ig) molecule. A typical Ig molecule is composed of two heavy chains and two light chains. The heavy chains can be divided into at least three functional regions: the Fd region, the Fc region (fragment crystallizable region), and the hinge region, the latter being found only in IgG, IgA, and IgD immunoglobulins. The Fd region comprises the variable (VH) and constant (CHi) domains of the heavy chains, and together with the variable (VL) and constant (CL) domains of the light chains forms the antigen-binding fragment or Fab region.

The Fc region of IgG, IgA, and IgD immunoglobulins comprises the heavy chain constant domains 2 and 3, designated respectively as CH ₂ and CH ₃ regions; and the Fc region of IgE and IgM immunoglobulins comprises the heavy chain constant domains 2, 3, and 4, designated respectively as CH ₂ , CH ₃ , and CH ₄ regions. The Fc region is mainly responsible for the immunoglobulin effector functions, which include, for example, complement fixation and binding to cognate Fc receptors of effector cells.

The hinge region (found in IgG, IgA, and IgD) acts as a flexible spacer that allows the Fab portion to move freely in space relative to the Fc region. In contrast to the constant regions, the hinge regions are structurally diverse, varying in both sequence and length among immunoglobulin classes and subclasses. The hinge region may also contain one or more glycosylation site(s), which include a number of structurally distinct types of sites for carbohydrate attachment. For example, IgAl contains five glycosylation sites within a 17 amino acid segment of the hinge region, conferring significant resistance of the hinge region polypeptide to intestinal proteases. Residues in the hinge proximal region of the CH ₂ domain can also influence the specificity of the interaction between an

immunoglobulin and its respective Fc receptor(s) (see, e.g., Shin et al., Intern. Rev. Immunol. 10: 177- 186, 1993).

The term "Fc region" or "Fc fragment" or "Fc" as used herein, thus refers to a protein that contains one or more of a CH ₂ region, a CH ₃ region, and/or a CH ₄ region from one or more selected immunoglobulin(s), including fragments and variants and combinations thereof. An "Fc region" may also include one or more hinge region(s) of the heavy chain constant region of an immunoglobulin. In certain embodiments, the Fc region does not contain one or more of the CHi, CL, VL, and/or VH regions of an immunoglobulin.

The Fc region can be derived from the CH ₂ region, CH ₃ region, CH ₄ region, and/or hinge region(s) of any one or more immunoglobulin classes, including but not limited to IgA, IgD, IgE, IgG, IgM, including subclasses and combinations thereof. In some embodiments, the Fc region is derived from an IgA immunoglobulin, including subclasses IgAl and/or IgA2. In certain embodiments, the Fc region is derived from an IgD immunoglobulin. In particular embodiments, the Fc region is derived from an IgE immunoglobulin. In some embodiments, the Fc region is derived from an IgG immunoglobulin, including subclasses IgGI, IgG2, IgG2, IgG3, and/or IgG4. In certain embodiments, the Fc region is derived from an IgM immunoglobulin. Certain Fc regions demonstrate specific binding for one or more Fc -receptors (FcRs).

Examples of classes of Fc receptors include Fey receptors (FcyR), Fca receptors (FcaR), Fee receptors (FceR), and the neonatal Fc receptor (FcRn). For instance, certain Fc regions have increased binding to (or affinity for) one or more FcyRs, relative to FcaRs, FceRs, and/or FcRn. In some embodiments, Fc regions have increased binding to FcaRs, relative to one or more FcyRs, FceRs, and/or FcRn. In other embodiments, Fc regions have increased binding to FceRs (e.g., FcaRI), relative to one or more FcyRs, FcaRs, and/or FcRn. In particular embodiments, Fc regions have increased binding to FcRn, relative to one or more FcyRs, FcaRs, and/or FceRs. In certain embodiments, the binding (or affinity) of an Fc region to one or more selected FcR(s) is increased relative to its binding to (or affinity for) one or more different FcR(s), typically by about 1.5x, 2x, 2.5x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, lOx, 15x, 20x, 25x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, lOOx, 200x, 300x, 400x, 500x, 600x, 700x, 800x, 900x, lOOOx or more (including all integers in between).

Examples of FcyRs include FcyRI, FcyRIIa, FcyRIIb, FcyRIIc, FcyRIIIa, and FcyRIIIb. FcyRI (CD64) is expressed on macrophages and dendritic cells and plays a role in phagocytosis, respiratory burst, cytokine stimulation, and dendritic cell endocytic transport. Expression of FcyRI is upregulated by both GM-CSF and y-interferon (y-IFN) and downregulated by interleukin-4 (IL-4). FcyRIIa is expressed on polymorphonuclear leukocytes (PMN), macrophages, dendritic cells, and mast cells. FcyRIIa plays a role in phagocytosis, respiratory burst, and cytokine stimulation. Expression of FcyRIIa is upregulated by GM-CSF and y-IFN, and decreased by IL-4. Fcyllb is expressed on B cells, PMN, macrophages, and mast cells. Fcyllb inhibits immunoreceptor tyrosine-based activation motif (IT AM) mediated responses, and is thus an inhibitory receptor. Expression of FcyRIIc is upregulated by intravenous immunoglobulin (IVIG) and IL-4 and decreased by y-IFN. FcyRIIc is expressed on NK cells. FcyRIIIa is expressed on natural killer (NK) cells, macrophages, mast cells, and platelets. This receptor participates in phagocytosis, respiratory burst, cytokine stimulation, platelet aggregation and degranulation, and NK-mediated ADCC. Expression of FcyRIII is upregulated by C5a, TGF-β, and y-IFN and downregulated by IL-4. Fc y RHIb is a GPI-linked receptor expressed on PMN.

Certain Fc regions have increased binding to FcyRI, relative to FcyRIIa, FcyRIIb, FcyRIIc, FcyRIIIa, and/or FcyRIIIb. Some embodiments have increased binding to FcyRIIa, relative to FcyRI, FcyRIIb, FcyRIIc, FcyRIIIa, and/or FcyRIIIb. Particular Fc regions have increased binding to FcyRIIb, relative to FcyRI, FcyRIIa, FcyRIIc, FcyRIIIa, and/or FcyRIIIb. Certain Fc regions have increased binding to FcyRIIc, relative to FcyRI, FcyRIIa, FcyRIIb, FcyRIIIa, and/or FcyRIIIb. Some Fc regions have increased binding to FcyRIIIa, relative to FcyRI, FcyRIIa, FcyRIIb, FcyRIIc, and/or FcyRIIIb. Specific Fc regions have increased binding to FcyRIIIb, relative to FcyRI, FcyRIIa, FcyRIIb, FcyRIIc, and/or FcyRIIIa.

FcaRs include FcaRI (CD89). FcaRI is found on the surface of neutrophils, eosinophils, monocytes, certain macrophages (e.g., Kupffer cells), and certain dendritic cells. FcaRI is composed of two extracellular Ig-like domains, is a member of both the immunoglobulin superfamily and the multi-chain immune recognition receptor (MIRR) family, and signals by associating with two FcRy signaling chains.

FceRs include FceRI and FceRII. The high-affinity receptor FceRI is a member of the immunoglobulin superfamily, is expressed on epidermal Langerhans cells, eosinophils, mast cells and basophils, and plays a major role in controlling allergic responses. FceRI is also expressed on antigen- presenting cells, and regulates the production pro-inflammatory cytokines. The low-affinity receptor FceRII (CD23) is a C-type lectin that can function as a membrane-bound or soluble receptor. FceRII regulates B cell growth and differentiation, and blocks IgE-binding of eosinophils, monocytes, and basophils. Certain Fc regions have increased binding to FceRI, relative to FceRII. Other Fc regions have increased binding to FceRII, relative to FceRI.

Table H6 below summarizes the characteristics of certain FcRs.

cell s

FcaRI IgA Low (Kd > lCT ⁶ Monocytes Phagocytosis

(CD89) M) Macrophages Induction of microbe

Neutrophils killing

Eosinophils

Fca/pR IgA and High for IgM, B cells Endocytosis

IgM Moderate for Mesangial Induction of microbe

IgA cell s killing

Macrophages

FcRn IgG Monocytes Transfers IgG from a

Macrophages mother to fetus through Dendrite the placenta

cell s Transfers IgG from a

Epithelial mother to infant in cell s milk

Endothelial Protects IgG from cell s degradation

Hepatocytes

Fc regions can be derived from the immunoglobulin molecules of any animal, including vertebrates such as mammals such cows, goats, swine, dogs, mice, rabbits, hamsters, rats, guinea pigs, non-human primates, and humans. The amino acid sequences of CH ₂ , CH ₃ , CH ₄ , and hinge regions from exemplary, wild-type human IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, and IgM immunoglobulins are shown in Table H7.

IgGl hinge EPKSCDKTHTCPPCP 140 modi fied SDKTHTCPPCP 141 human IgGl

hinge

IgGl CH2 APELLGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPEVKFNWYVDG 142

VEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKAK

IgGl CH3 GQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNY 143

KTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPGK

IgGl heavy MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHE 144 chain DPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYK

CKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKG FYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK

IgG2 hinge ERKCCVECPPCP 145

IgG2 CH2 APPVAGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVQFNWYVDGV 146

EVHNAKTKPREEQFNSTFRWSVLTWHQDWLNGKEYKCKVSNKGLPAPIE KTISKTK

IgG2 CH3 GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNY 147

KTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPGK

IgG3 hinge ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKS 148

CDTPPPCPRCP

IgG3 CH2 APELLGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPEVQFKWYVDG 149

VEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKTK

IgG3 CH3 GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNY 150

NTTPPMLDSDGSFFLYSKLTVDKSRWQQGNI FSCSVMHEALHNRFTQKSLS LSPGK

IgG4 hinge ESKYGPPCPSCP 151

IgG4 CH2 APEFLGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSQEDPEVQFNWYVDG 152

VEVHNAKTKPREEQFNSTYRWSVLTVLHQDWLNGKEYKCKVSNKGLPSSI EKTISKAK

IgG4 CH3 GQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNY 153

KTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLS LSLGK

IgM CH2 VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQV 154

GSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTF QQNASSMCVP

IgM CH3 DQDTAIRVFAI PPSFASI FLTKSTKLTCLVTDLTTYDSVTI SWTRQNGEAV 155

KTHTNI SESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTI SRPK

IgM CH4 GVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLS 156

PEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCWAHEALPNRV TERTVDKSTGKPTLYNVSLVMSDTAGTCY

An Fc region of an HRS-Fc conjugate can thus comprise, consist of, or consist essentially of one or more of the human Fc region amino acid sequences of Table H7, including variants, fragments, homologs, orthologs, paralogs, and combinations thereof. Certain illustrative embodiments comprise an Fc region that ranges in size from about 20-50, 20-100, 20-150, 20-200, 20-250, 20-300, 20-400, 50-100, 50-150, 50-200, 50-250, 50-300, 50-400, 100-150, 100-200, 100-250, 100-300, 100- 350, 100-400, 200-250, 200-300, 200-350, or 200-400 amino acids in length, and optionally comprises, consists of, or consists essentially of any one or more of the sequences in Table H7. Certain embodiments comprise an Fc region of up to about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 300, 350, 400 or more amino acids, which optionally comprises, consists of, or consists essentially of any one or more of the amino acid sequences of Table H7.

Certain Fc regions comprise, consist of, or consist essentially of human IgAl sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof, and variants and fragments thereof. Certain Fc regions comprise, consist of, or consist essentially of human the IgAl sequence of Table H7. Certain Fc regions comprise, consist of, or consist essentially of the human IgAl sequence of Table H7. Certain Fc regions comprise, consist of, or consist essentially of the human IgAl sequence of Table H7.

Some Fc regions comprise, consist of, or consist essentially of human IgA2 sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof, and variants and fragments thereof. Certain Fc regions comprise, consist of, or consist essentially of human the IgA2 sequence of Table H7. Certain Fc regions comprise, consist of, or consist essentially of the human IgA2 sequence of Table H7. Certain Fc regions comprise, consist of, or consist essentially of the human IgA2 sequence of Table H7.

Certain Fc regions comprise, consist of, or consist essentially of human IgD sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof, and variants and fragments of these sequences and combinations. Certain Fc regions comprise, consist of, or consist essentially of human IgE sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof, and variants and fragments of these sequences and combinations. Certain Fc regions comprise, consist of, or consist essentially of human IgGl sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof, and variants and fragments of these sequences and combinations. Certain Fc regions comprise, consist of, or consist essentially of human IgG2 sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof. Certain Fc regions comprise, consist of, or consist essentially of human IgG3 sequences of Table H7, in any order reading from N- terminus to C-terminus, including combinations thereof. Certain Fc regions comprise, consist of, or consist essentially of human IgG4 sequences of Table H7, in any order reading from N-terminus to C-terminus, including combinations thereof Certain Fc regions comprise, consist of, or consist essentially of human IgM sequences of Table H7, in any order reading from N-terminus to C- terminus, including combinations thereof, and variants and fragments of these sequences and combinations.

Exemplary HRS-Fc fusion conjugates are provided in Table H8 below.

SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT

VDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAERAALEELVKL QGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK

Fc-HRS (2-60) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 158

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK

HRS (1-60) -Fc MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 159

ESKQKFVLKTPKSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRW SVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTL PPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

Fc-HRS (2-60) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 160

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK

HRS (1-60) -Fc MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 161

ESKQKFVLKTPKSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRW SVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTL PPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

Fc-HRS (2-40) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 162

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLK

Fc-HRS (2-45) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 163

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQL

Fc-HRS (2-50) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 164

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDES

Fc-HRS (2-55) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 165

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFV

Fc-HRS (2-66) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDV 166

SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK GTRDYS

HRS (1-40) -Fc MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKSDKTHTCP 167

PCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVWDVSHEDPEVKF NWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKG

FYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQ GNVFSCSVMHEALHNHYTQKSLSLSPGK

HRS (1-45) -Fc MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLSDK 168

THTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHED PEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKE YKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

HRS (1-50) -Fc MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 169

ESSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVWD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDW LNGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKN QVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

HRS (1-55) -Fc MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 170

ESKQKFVSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVT CVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTV LHQDWLNGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSRE EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

HRS (1-66) -Fc MAEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPD 171

ESKQKFVLKTPKGTRDYSSDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMI SRTPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYN STYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTI SKAKGQPRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKT TPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSL SLSPGK

Fc-HRS (2-60) MSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVWDV 172 HRS (2-60) SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWL

NGKEYKCKVSNKALPAPIEKTI SKAKGQPREPQVYTLPPSREEMTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEPAALEELVK LQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPK AEPAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDE SKQKFVLKTPK

Accordingly, in certain embodiments, the HRS polypeptide is fused or otherwise conjugated to an Fc region and comprises, consists, or consists essentially of an amino acid sequence in Table H8 (SEQ ID NO: 157-172) or an active variant or fragment thereof. In some embodiments, the expressible polynucleotide encodes an HRS polypeptide that comprises consists, or consists essentially of an amino acid sequence in Table H8 (e.g., SEQ ID NO: 157-172) or an active variant or fragment thereof.

As noted above, certain embodiments employ variants, fragments, hybrids, and/or otherwise modified forms an Fc region described herein and known in the art. Included are variants having one or more amino acid substitutions, insertions, deletions, and/or truncations relative to a reference sequence, such as any one or more of the reference sequences of Table H7 or Table H8. Polypeptide and polynucleotide variants are described elsewhere herein.

Also included are hybrid Fc regions, for example, Fc regions that comprise a combination of Fc domains (e.g., hinge, C¾, C¾, CH4) from immunoglobulins of different species, different Ig classes, and/or different Ig subclasses. General examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of CH ₂ /CH ₃ domains: IgAl/IgAl, IgAl IgA2, IgAl/IgD, IgAl/IgE, IgAl/IgGl, IgAl/IgG2, IgAl/IgG3, IgAl/IgG4, IgAl/IgM, IgA2/IgAl, IgA2/IgA2, IgA2/IgD, IgA2/IgE, IgA2/IgGl, IgA2/IgG2, IgA2/IgG3, IgA2/IgG4, IgAMgM, IgD/IgAl, IgD/IgA2, IgD/IgD, IgD/lgE, IgD/IgGl, IgD/IgG2, IgD/IgG3, IgD/IgG4, IgD/IgM, IgE/IgAl, IgE/IgA2, IgE/lgD, IgE/IgE, IgE/IgGl, IgE/IgG2, IgE/IgG3, IgE/IgG4, IgE/IgM, IgGl/IgAl, IgGl/IgA2, IgGl/IgD, IgGl/lgE, IgGl/IgGl, IgGl/IgG2, IgGl/IgG3, IgGl/IgG4, IgGl/IgM, IgG2/IgAl, IgG2/IgA2, IgG2/IgD, IgG2/IgE, IgG2/IgGl, IgG2/IgG2, IgG2/IgG3, IgG2/IgG4, IgG2/IgM, IgG3/IgAl, IgG3/IgA2, IgG3/IgD, IgG3/IgE, IgG3/IgGl, IgG3/IgG2, IgG3/IgG3, IgG3/IgG4, IgG3/IgM, IgG4/IgAl, IgG4/IgA2, IgG4/IgD, IgG4/IgE, IgG4/IgGl, IgG4/IgG2, IgG4/IgG3, IgG4/IgG4, IgG4/IgM, IgM/IgAl, IgM/IgA2, IgM/IgD, IgM/IgE, IgM/IgGl, IgM/IgG2, IgM/IgG3, IgM/IgG4, IgM/IgM (or fragments or variants thereof), and optionally include a hinge from one or more of IgAl, IgA2, IgD, IgGl, IgG2, IgG3, or IgG4, and/or a CH ₄ domain from IgE and/or IgM. In specific embodiments, the hinge, CH ₂ , CH ₃ , and CH ₄ domains are from human Ig.

Additional examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of CH ₂ /CH ₄ domains: IgAl/IgE, IgA2/IgE, IgD/lgE, IgE/IgE, IgGl/lgE, IgG2/IgE, IgG3/IgE, IgG4/IgE, IgM/IgE, IgAl/IgM, IgA2/IgM, IgD/IgM, IgE/IgM, IgGl/IgM, IgG2/IgM, IgG3/IgM, IgG4/IgM, IgM/IgM (or fragments or variants thereof), and optionally include a hinge from one or more of IgAl, IgA2, IgD, IgGl, IgG2, IgG3, IgG4, and/or a CH ₃ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM. In specific embodiments, the hinge, CH ₂ , CH ₃ , and CH ₄ domains are from human Ig.

Certain examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of CH ₃ /CH ₄ domains: IgAl/IgE, IgA2/IgE, IgD/lgE, IgE/IgE, IgGl/lgE, IgG2/IgE, IgG3/IgE, IgG4/IgE, IgM/IgE, IgAl/IgM, IgA2/IgM, IgD/IgM, IgE/IgM, IgGl/IgM, IgG2/IgM, IgG3/IgM, IgG4/IgM, IgM/IgM (or fragments or variants thereof), and optionally include a hinge from one or more of IgAl, IgA2, IgD, IgGl, IgG2, IgG3, IgG4, and/or a CH ₂ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM. In specific embodiments, the hinge, CH ₂ , CH ₃ , and CH domains are from human Ig.

Particular examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of hinge/CH ₂ domains: IgAl/IgAl, IgAl/IgA2, IgAl/IgD, IgAl/IgE, IgAMgGl, IgAl/IgG2, IgAl/IgG3, IgAl/IgG4, IgAl/IgM, IgA2/IgAl, IgA2/IgA2, IgA2/IgD, IgA2/IgE, IgA2/IgGl, IgA2/IgG2, IgA2/IgG3, IgA2/IgG4, IgA2/IgM, IgD/IgAl, IgD/IgA2, IgD/IgD, IgD/lgE, IgD/IgGl, IgD/IgG2, IgD/IgG3, IgD/IgG4, IgD/IgM, IgGl/IgAl, IgGl/IgA2, IgGl/IgD, IgGl/lgE, IgGl/IgGl, IgGl/IgG2, IgGl/IgG3, IgGl/IgG4, IgGl/IgM, IgG2/IgAl, IgG2/IgA2, IgG2/IgD, IgG2/IgE, IgG2/IgGl, IgG2/IgG2, IgG2/IgG3, IgG2/IgG4, IgG2/IgM, IgG3/IgAl, IgG3/IgA2, IgG3/IgD, IgG3/IgE, IgG3/IgGl, IgG3/IgG2, IgG3/IgG3, IgG3/IgG4, IgG3/IgM, IgG4/IgAl, IgG4/IgA2, IgG4/IgD, IgG4/IgE, IgG4/IgGl, IgG4/IgG2, IgG4/IgG3, IgG4/IgG4, IgG4/IgM (or fragments or variants thereof), and optionally include a CH ₃ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM, and/or a CH ₄ domain from IgE and/or IgM. In specific embodiments, the hinge, CH ₂ , CH ₃ , and CH ₄ domains are from human Ig.

Certain examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of hinge/CH ₃ domains: IgAl/IgAl, IgAl/IgA2, IgAl/IgD, IgAl/IgE, IgAMgGl, IgAl/IgG2, IgAl/IgG3, IgAl/IgG4, IgAl/IgM, IgA2/IgAl, IgA2/IgA2, IgA2/IgD, IgA2/IgE, IgA2/IgGl, IgA2/IgG2, IgA2/IgG3, IgA2/IgG4, IgA2/IgM, IgD/IgAl, IgD/IgA2, IgD/IgD, IgD/IgE, IgD/IgGl, IgD/IgG2, IgD/IgG3, IgD/IgG4, IgD/IgM, IgGl/IgAl, IgGl/IgA2, IgGl/IgD, IgGl/IgE, IgGl/IgGl, IgGl/IgG2, IgGl/IgG3, IgGl/IgG4, IgGl/IgM, IgG2/IgAl, IgG2/IgA2, IgG2/IgD, IgG2/IgE, IgG2/IgGl, IgG2/IgG2, IgG2/IgG3, IgG2/IgG4, IgG2/IgM, IgG3/IgAl, IgG3/IgA2, IgG3/IgD, IgG3/IgE, IgG3/IgGl, IgG3/IgG2, IgG3/IgG3, IgG3/IgG4, IgG3/IgM, IgG4/IgAl, IgG4/IgA2, IgG4/IgD, IgG4/IgE, IgG4/IgGl, IgG4/IgG2, IgG4/IgG3, IgG4/IgG4, IgG4/IgM (or fragments or variants thereof), and optionally include a CH ₂ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM, and/or a CH ₄ domain from IgE and or IgM. In specific embodiments, the hinge, CH ₂ , CH ₃ , and CH ₄ domains are from human Ig.

Some examples include hybrid Fc regions that comprise, consist of, or consist essentially of the following combination of hinge/CH ₄ domains: IgAl/IgE, IgAl/IgM, IgA2/IgE, IgA2/IgM, IgD/IgE, IgD/IgM, IgGl/IgE, IgGl/IgM, IgG2/IgE, IgG2/IgM, IgG3/IgE, IgG3/IgM, IgG4/IgE, IgG4/IgM (or fragments or variants thereof), and optionally include a CH ₂ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM, and/or a CH ₃ domain from one or more of IgAl, IgA2, IgD, IgE, IgGl, IgG2, IgG3, IgG4, or IgM.

Specific examples of hybrid Fc regions can be found, for example, in WO 2008/147143, which are derived from combinations of IgG subclasses or combinations of human IgD and IgG.

Also included are derivatized or otherwise modified Fc regions. In certain aspects, the Fc region may be modified by phosphorylation, sulfation, acrylation, glycosylation, methylation, farnesylation, acetylation, amidation, and the like, for instance, relative to a wild-type or naturally- occurring Fc region. In certain embodiments, the Fc region may comprise wild-type or native glycosylation patterns, or alternatively, it may comprise increased glycosylation relative to a native form, decreased glycosylation relative to a native form, or it may be entirely deglycosylated. As one example of a modified Fc glycoform, decreased glycosylation of an Fc region reduces binding to the Clq region of the first complement component CI, a decrease in ADCC-related activity, and/or a decrease in CDC-related activity. Certain embodiments thus employ a deglycosylated or aglycosylated Fc region. See, e.g., WO 2005/047337 for the production of exemplary aglycosylated Fc regions. Another example of an Fc region glycoform can be generated by substituting the Q295 position with a cysteine residue (see, e.g., U.S. Application No. 2010/0080794), according to the Kabat et al. numbering system. Certain embodiments may include Fc regions where about 80-100% of the glycoprotein in Fc region comprises a mature core carbohydrate structure that lacks fructose (see, e.g., U.S. Application No. 2010/0255013). Some embodiments may include Fc regions that are optimized by substitution or deletion to reduce the level of fucosylation, for instance, to increase affinity for FcyRI, FcyRIa, or FcyRIIIa, and/or to improve phagocytosis by FcyRIIa-expressing cells (see U.S. Application Nos. 2010/0249382 and 2007/0148170).

As another example of a modified Fc glycoform, an Fc region may comprise oligomannose- type N-glycans, and optionally have one or more of the following: increased ADCC activity, increased binding affinity for FcyRIIIA (and certain other FcRs), similar or increased binding specificity for the target of the HRS polypeptide, similar or higher binding affinity for the target of the HRS polypeptide, and/or similar or lower binding affinity for mannose receptor, relative to a corresponding Fc region or HRS-Fc conjugate that contains complex-type N-glycans (see, e.g., U.S. Application No. 2007/0092521 and U.S. Patent No. 7,700,321). As another example, enhanced affinity of Fc regions for FcyRs has been achieved using engineered glycoforms generated by expression of antibodies in engineered or variant cell lines (see, e.g., Umana et al , Nat Biotechnol. 17: 176-180, 1999; Davies et al, Biotechnol Bioeng. 74:288-294, 2001; Shields et al, J Biol Chem. 277:26733-26740, 2002; Shinkawa et al, 2003, J Biol Chem. 278:3466-3473, 2003; and U.S.

Application No. 2007/0111281). Certain Fc region glycoforms comprise an increased proportion of N-glycoside bond type complex sugar chains, which do not have the 1 -position of fucose bound to the 6-position of N-acetylglucosamine at the reducing end of the sugar chain (see, e.g., U.S. Application No. 2010/0092997). Particular embodiments may include IgG Fc region that is glycosylated with at least one galactose moiety connected to a respective terminal sialic acid moiety by an a-2,6 linkage, optionally where the Fc region has a higher anti-inflammatory activity relative to a corresponding, wild-type Fc region (see U.S. Application No. 2008/0206246). Certain of these and related altered glycosylation approaches have generated substantial enhancements of the capacity of Fc regions to selectively bind FcRs such as FcyRIII, to mediate ADCC, and to alter other properties of Fc regions, as described herein.

Certain variant, fragment, hybrid, or otherwise modified Fc regions may have altered binding to one or more FcRs, relative to a corresponding, wild-type Fc sequence (e.g., same species, same Ig class, same Ig subclass). For instance, such Fc regions may have increased binding to one or more of Fey receptors, Fca receptors, Fee receptors, and/or the neonatal Fc receptor, relative to a corresponding, wild-type Fc sequence. In other embodiments, variant, fragment, hybrid, or modified Fc regions may have decreased binding to one or more of Fey receptors, Fca receptors, Fee receptors, and/or the neonatal Fc receptor, relative to a corresponding, wild-type Fc sequence. Specific FcRs are described elsewhere herein.

Specific examples of Fc variants having altered (e.g., increased, decreased) FcR binding can be found, for example, in U.S. Pat. Nos. 5,624,821 and 7,425,619; U.S. Application Nos.

2009/0017023, 2009/0010921, and 2010/0203046; and WO 2000/42072 and WO 2004/016750. Certain examples include human Fc regions having a one or more substitutions at position 298, 333, and/or 334, for example, S298A, E333 A, and/or K334A (based on the numbering of the EU index of Kabat et al), which have been shown to increase binding to the activating receptor FcyRIIIa and reduce binding to the inhibitory receptor FcyRIIb. These mutations can be combined to obtain double and triple mutation variants that have further improvements in binding to FcRs. Certain embodiments include a S298A/E333A/K334A triple mutant, which has increased binding to FcyRIIIa, decreased binding to FcyRIIb, and increased ADCC (see, e.g., Shields et al, J Biol Chem. 276:6591-6604, 2001; and Presta et al, Biochem Soc Trans. 30:487-490, 2002). See also engineered Fc glycoforms that have increased binding to FcRs, as disclosed in Umana et al, supra; and U.S. Patent No. 7,662,925. Some embodiments include Fc regions that comprise one or more substitutions selected from 434S, 252Y/428L, 252Y/434S, and 428L/434S (see U.S. Application Nos. 2009/0163699 and

20060173170), based on the EU index of Kabat et al.

Certain variant, fragment, hybrid, or modified Fc regions may have altered effector functions, relative to a corresponding, wild-type Fc sequence. For example, such Fc regions may have increased complement fixation or activation, increased Clq binding affinity, increased CDC-related activity, increased ADCC-related activity, and/or increased ADCP-related activity, relative to a corresponding, wild-type Fc sequence. In other embodiments, such Fc regions may have decreased complement fixation or activation, decreased Clq binding affinity, decreased CDC-related activity, decreased ADCC-related activity, and/or decreased ADCP-related activity, relative to a corresponding, wild- type Fc sequence. As merely one illustrative example, an Fc region may comprise a deletion or substitution in a complement-binding site, such as a Clq-binding site, and/or a deletion or substitution in an ADCC site. Examples of such deletions/substitutions are described, for example, in U.S. Patent No. 7,030,226. Many Fc effector functions, such as ADCC, can be assayed according to routine techniques in the art. (see, e.g., Zuckerman et al, CRC Crit Rev Microbiol. 7: 1-26, 1978). Useful effector cells for such assays includes, but are not limited to, natural killer (NK) cells, macrophages, and other peripheral blood mononuclear cells (PBMC). Alternatively, or additionally, certain Fc effector functions may be assessed in vivo, for example, by employing an animal model described in Clynes et al. PNAS. 95:652-656, 1998.

Certain variant hybrid, or modified Fc regions may have altered stability or half-life relative to a corresponding, wild-type Fc sequence. In certain embodiments, such Fc regions may have increased half -life relative to a corresponding, wild-type Fc sequence. In other embodiments, variant hybrid, or modified Fc regions may have decreased half-life relative to a corresponding, wild-type Fc sequence. Half-life can be measured in vitro (e.g., under physiological conditions) or in vivo, according to routine techniques in the art, such as radiolabeling, ELISA, or other methods. In vivo measurements of stability or half-life can be measured in one or more bodily fluids, including blood, serum, plasma, urine, or cerebrospinal fluid, or a given tissue, such as the liver, kidneys, muscle, central nervous system tissues, bone, etc. As one example, modifications to an Fc region that alter its ability to bind the FcRn can alter its half-life in vivo. Assays for measuring the in vivo

pharmacokinetic properties (e.g., in vivo mean elimination half -life) and non-limiting examples of Fc modifications that alter its binding to the FcRn are described, for example, in U.S. Pat. Nos. 7,217,797 and 7,732,570; and U.S. Application Nos. US 2010/0143254 and 2010/0143254.

Additional non-limiting examples of modifications to alter stability or half-life include substitutions/deletions at one or more of amino acid residues selected from 251-256, 285-290, and 308-314 in the CH ₂ domain, and 385-389 and 428-436 in the CH ₃ domain, according to the numbering system of Kabat et al. See U.S. Application No. 2003/0190311. Specific examples include substitution with leucine at position 251, substitution with tyrosine, tryptophan or phenylalanine at position 252, substitution with threonine or serine at position 254, substitution with arginine at position 255, substitution with glutamine, arginine, serine, threonine, or glutamate at position 256, substitution with threonine at position 308, substitution with proline at position 309, substitution with serine at position 311, substitution with aspartate at position 312, substitution with leucine at position 314, substitution with arginine, aspartate or serine at position 385, substitution with threonine or proline at position 386, substitution with arginine or proline at position 387, substitution with proline, asparagine or serine at position 389, substitution with methionine or threonine at position 428, substitution with tyrosine or phenylalanine at position 434, substitution with histidine, arginine, lysine or serine at position 433, and/or substitution with histidine, tyrosine, arginine or threonine at position 436, including any combination thereof. Such modifications optionally increase affinity of the Fc region for the FcRn and thereby increase half-life, relative to a corresponding, wild-type Fc region.

Certain variant hybrid, or modified Fc regions may have altered solubility relative to a corresponding, wild-type Fc sequence. In certain embodiments, such Fc regions may have increased solubility relative to a corresponding, wild-type Fc sequence. In other embodiments, variant hybrid, or modified Fc regions may have decreased solubility relative to a corresponding, wild-type Fc sequence. Solubility can be measured, for example, in vitro (e.g., under physiological conditions) according to routine techniques in the art. Exemplary solubility measurements are described elsewhere herein.

Additional examples of variants include IgG Fc regions having conservative or non- conservative substitutions (as described elsewhere herein) at one or more of positions 250, 314, or 428 of the heavy chain, or in any combination thereof, such as at positions 250 and 428, or at positions 250 and 314, or at positions 314 and 428, or at positions 250, 314, and 428 (see, e.g., U.S. Application No. 2011/0183412). In specific embodiments, the residue at position 250 is substituted with glutamic acid or glutamine, and/or the residue at position 428 is substituted with leucine or phenylalanine. As another illustrative example of an IgG Fc variant, any one or more of the amino acid residues at positions 214 to 238, 297 to 299, 318 to 322, and/or 327 to 331 may be used as a suitable target for modification (e.g., conservative or non-conservative substitution, deletion). In particular embodiments, the IgG Fc variant CH ₂ domain contains amino acid substitutions at positions 228, 234, 235, and/or 331 (e.g., human IgG4 with Ser228Pro and Leu235Ala mutations) to attenuate the effector functions of the Fc region (see U.S. Patent No. 7,030,226). Here, the numbering of the residues in the heavy chain is that of the EU index (see Kabat et al. , "Sequences of Proteins of Immunological Interest," 5 ^th Ed., National Institutes of Health, Bethesda, Md. (1991)). Certain of these and related embodiments have altered (e.g., increased, decreased) FcRn binding and/or serum half-life, optionally without reduced effector functions such as ADCC or CDC-related activities.

Additional examples include variant Fc regions that comprise one or more amino acid substitutions at positions 279, 341, 343 or 373 of a wild-type Fc region, or any combination thereof (see, e.g., U.S. Application No. 2007/0224188). The wild-type amino acid residues at these positions for human IgG are valine (279), glycine (341), proline (343) and tyrosine (373). The substation(s) can be conservative or non-conservative, or can include non-naturally occurring amino acids or mimetics, as described herein. Alone or in combination with these substitutions, certain embodiments may also employ a variant Fc region that comprises at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more amino acid substitutions selected from the following: 235G, 235R, 236F, 236R, 236Y, 237K, 237N, 237R, 238E, 238G, 238H, 2381, 238L, 238V, 238W, 238Y, 244L, 245R, 247 A, 247D, 247E, 247F, 247M, 247N, 247Q, 247R, 247S, 247T, 247W, 247Y, 248F, 248P, 248Q, 248W, 249L, 249M, 249N, 249P, 249Y, 251H, 2511, 251W, 254D, 254E, 254F, 254G, 254H, 2541, 254K, 254L, 254M, 254N, 254P, 254Q, 254R, 254V, 254W, 254Y, 255K, 255N, 256H, 2561, 256K, 256L, 256V, 256W, 256Y, 257A, 2571, 257M, 257N, 257S, 258D, 260S, 262L, 264S, 265K, 265S, 267H, 2671, 267K, 268K, 269N, 269Q, 271T, 272H, 272K, 272L, 272R, 279A, 279D, 279F, 279G, 279H, 2791, 279K, 279L, 279M, 279N, 279Q, 279R, 279S, 279T, 279W, 279Y, 280T, 283F, 283G, 283H, 2831, 283K, 283L, 283M, 283P, 283R, 283T, 283 W, 283 Y, 285N, 286F, 288N, 288P, 292E, 292F, 292G, 2921, 292L, 293 S, 293V, 301 W, 304E, 307E, 307M, 312P, 315F, 315K, 315L, 315P, 315R, 316F, 316K, 317P, 317T, 318N, 318P, 318T, 332F, 332G, 332L, 332M, 332S, 332V, 332W, 339D, 339E, 339F, 339G, 339H, 3391, 339K, 339L, 339M, 339N, 339Q, 339R, 339S, 339W, 339Y, 341D, 341E, 341F, 341H, 3411, 341K, 341L, 341M, 341N, 341P, 341Q, 341R, 341S, 341T, 341V, 341W, 341Y, 343A, 343D, 343E, 343F, 343G, 343H, 3431, 343K, 343L, 343M, 343N, 343Q, 343R, 343S, 343T, 343V, 343W, 343Y, 373D, 373E, 373F, 373G, 373H, 3731, 373K, 373L, 373M, 373N, 373Q, 373R, 373S, 373T, 373V, 373W, 375R, 376E, 376F, 376G, 376H, 3761, 376L, 376M, 376N, 376P, 376Q, 376R, 376S, 376T, 376V, 376W, 376Y, 377G, 377K, 377P, 378N, 379N, 379Q, 379S, 379T, 380D, 380N, 380S, 380T, 382D, 382F, 382H, 3821, 382K, 382L, 382M, 382N, 382P, 382Q, 382R, 382S, 382T, 382V, 382W, 382Y, 385E, 385P, 386K, 423N, 424H, 424M, 424V, 426D, 426L, 427N, 429A, 429F, 429M, 430A, 430D, 430F, 430G, 430H, 4301, 430K, 430L, 430M, 430N, 430P, 430Q, 430R, 430S, 430T, 430V, 430W, 430Y, 431H, 43 IK, 43 IP, 432R, 432S, 438G, 438K, 438L, 438T, 438W, 439E, 439H, 439Q, 440D, 440E, 440F, 440G, 440H, 4401, 440K, 440L, 440M, 440Q, 440T, 440V or 442K. As above, the numbering of the residues in the heavy chain is that of the EU index (see Kabat et al, supra). Such variant Fc regions typically confer an altered effector function or altered serum half-life upon HRS polypeptide to which the variant Fc region is operably attached. Preferably the altered effector function is an increase in ADCC, a decrease in ADCC, an increase in CDC, a decrease in CDC, an increase in Clq binding affinity, a decrease in Clq binding affinity, an increase in FcR (preferably FcRn) binding affinity or a decrease in FcR (preferably FcRn) binding affinity as compared to a corresponding Fc region that lacks such amino acid substitutions).

Additional examples include variant Fc regions that comprise an amino acid substitution at one or more of position(s) 221, 222, 224, 227, 228, 230, 231, 223, 233, 234, 235, 236, 237, 238, 239, 240, 241, 243, 244, 245, 246, 247, 249, 250, 258, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 278, 280, 281, 283, 285, 286, 288, 290, 291, 293, 294, 295, 296, 297, 298, 299, 300, 302, 313, 317, 318, 320, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335 336 and/or 428 (see, e.g., U.S. Patent No. 7,662,925). In specific embodiments, the variant Fc region comprises at least one amino acid substitution selected from the group consisting of: P230A, E233D, L234E, L234Y, L234I, L235D, L235S, L235Y, L235I, S239D, S239E, S239N, S239Q, S239T, V240I, V240M, F243L, V264I, V264T, V264Y, V266I, E272Y, K274T, K274E, K274R, K274L, K274Y, F275W, N276L, Y278T, V302I, E318R, S324D, S324I, S324V, N325T, K326I, K326T, L328M, L328I, L328Q, L328D, L328V, L328T, A330Y, A330L, A330I, I332D, I332E, I332N, I332Q, T335D, T335R, and T335Y. In other specific embodiments, the variant Fc region comprises at least one amino acid substitution selected from the group consisting of: V264I, F243L/V264I, L328M, I332E, L328M/I332E, V264I/I332E, S298A/I332E, S239E/I332E,

S239Q/I332E, S239E, A330Y, I332D, L328I/I332E, L328Q/I332E, V264T, V240I, V266I, S239D, S239D/I332D, S239D/I332E, S239D/I332N, S239D/I332Q, S239E/I332D, S239E/I332N,

S239E/I332Q, S239N/I332D, S239N/I332E, S239Q/I332D, A330Y/I332E, V264I/A330Y/I332E, A330L/I332E, V264I/A330L/I332E, L234E, L234Y, L234I, L235D, L235S, L235Y, L235I, S239T, V240M, V264Y, A330I, N325T, L328D/I332E, L328V/I332E, L328T/I332E, L328I/I332E, S239E/V264I/I332E, S239Q/V264I/I332E, S239E/V264I/A330Y/I332E, S239D/A330Y/I332E, S239N/A330Y/I332E, S239D/A330L/I332E, S239N/A330L/I332E, V264I/S298A/I332E,

S239D/S298A/I332E, S239N/S298A/I332E, S239D/V264I/I332E, S239D/V264I/S298A/I332E, S239D/V264I/A330L/I332E, S239D/I332E/A330I, P230A, P230A/E233D/I332E, E272Y, K274T, K274E, K274R, K274L, K274Y, F275W, N276L, Y278T, V302I, E318R, S324D, S324I, S324V, K326I, K326T, T335D, T335R, T335Y, V240I/V266I, S239D/A330Y/I332E/L234I,

S239D/A330Y/I332E/L235D, S239D/A330Y/I332E/V240I, S239D/A330Y/I332E/V264T, S239D/A330Y/I332E/ 326E, and S239D/A330Y/I332E/ 326T, In more specific embodiments, the variant Fc region comprises a series of substitutions selected from the group consisting of:

N297D/I332E, F241Y/F243Y/V262T/V264T/N297D/I332E, S239D/N297D/I332E,

S239E/N297D/I332E, S239D/D265Y/N297D/I332E, S239D/D265H/N297D/I332E,

V264E/N297D/I332E, Y296N/N297D/I332E, N297D/A330Y/I332E, S239D/D265V/N297D/I332E, S239D/D265I/N297D/I332E, and N297D/S298A/A330Y/I332E. In specific embodiments, the variant Fc region comprises an amino acid substitution at position 332 (using the numbering of the EU index, Kabat e/ a/., supra). Examples of substitutions include 332A, 332D, 332E, 332F, 332G, 332H, 332K, 332L, 332M, 332N, 332P, 332Q, 332R, 332S, 332T, 332V, 332W and 332Y. The numbering of the residues in the Fc region is that of the EU index of Kabat et al. Among other properties described herein, such variant Fc regions may have increased affinity for an FcyR, increased stability, and/or increased solubility, relative to a corresponding, wild-type Fc region.

Further examples include variant Fc regions that comprise one or more of the following amino acid substitutions: 224N/Y, 225A, 228L, 230S, 239P, 240A, 241L, 243S L/G H/I, 244L, 246E, 247L/A, 252T, 254T/P, 258K, 261Y, 265V, 266 A, 267G/N, 268N, 269K/G, 273 A, 276D, 278H, 279M, 280N, 283G, 285R, 288R, 289A, 290E, 291L, 292Q, 297D, 299A, 300H, 301C, 304G, 305A, 306I/F, 311R, 312N, 315D/ /S, 320R, 322E, 323A, 324T, 325S, 326E/R, 332T, 333D/G, 3351, 338R, 339T, 340Q, 341E, 342R, 344Q, 347R, 35 IS, 352A, 354A, 355W, 356G, 358T, 361D/Y, 362L, 364C, 365Q/P, 370R, 372L, 377V, 378T, 383N, 389S, 390D, 391C, 393A, 394A, 399G, 404S, 408G, 409R, 4111, 412A, 414M, 421S, 4221, 426F/P, 428T, 430K, 43 IS, 432P, 433P, 438L, 439E/R, 440G, 44 IF, 442T, 445R, 446 A, 447E, optionally where the variant has altered recognition of an Fc ligand and/or altered effector function compared with a parent Fc polypeptide, and wherein the numbering of the residues is that of the EU index as in Kabat et al. Specific examples of these and related embodiments include variant Fc regions that comprise or consist of the following sets of substitutions:

(I) N276D, R292Q, V305A, I377V, T394A, V412A and K439E; (2) P244L, K246E, D399G and K409R; (3) S304G, K320R, S324T, K326E and M358T; (4) F243S, P247L, D265V, V266A, S383N and T411I; (5) H224N, F243L, T393A and H433P; (6) V240A, S267G, G341E and E356G; (7) M252T, P291L, P352A, R355W, N390D, S408G, S426F and A431S; (8) P228L, T289A, L365Q, N389S and 5440G; (9) F241L, V273A, K340Q and L441F; (10) F241L, T299A, I332T and M428T;

(I I) E269K, Y300H, Q342R, V422I and G446A; (12) T225A, R301c, S304G, D312N, N315D, L351S and N421S; (13) S254T, L306I, K326R and Q362L; (14) H224Y, P230S, V323A, E333D, K338R and S364C; (15) T335I, K414M and P445R; (16) T335I and K414M; (17) P247A, E258K, D280N, K288R, N297D, T299A, K322E, Q342R, S354A and L365P; (18) H268N, V279M, A339T, N361D and S426P; (19) C261Y, K290E, L306F, Q311R, E333G and Q438L; (20) E283G, N315K, E333G, R344Q, L365P and S442T; (21) Q347R, N361Y and K439R; (22) S239P, S254P, S267N, H285R, N315S, F372L, A378T, N390D, Y391C, F404S, E430K, L432P and K447E; and (23) E269G, Y278H, N325S and K370R, wherein the numbering of the residues is that of the EU index as in Kabat et al. {see, e.g., U.S. Application No. 2010/0184959).

Another specific example of an Fc variant comprises an Fc sequence of Table H7, wherein Xaa at position 1 is Ala or absent; Xaa at position 16 is Pro or Glu; Xaa at position 17 is Phe, Val, or Ala; Xaa at position 18 is Leu, Glu, or Ala; Xaa at position 80 is Asn or Ala; and/or Xaa at position 230 is Lys or is absent (see, e.g., U.S. Application No. 2007/0253966). Certain of these Fc regions, and related HRS-Fc conjugates, have increased half-life, reduced effector activity, and/or are significantly less immunogenic than wild-type Fc sequences. Variant Fc regions can also have one or more mutated hinge regions, as described, for example, in U.S. Application No. 2003/0118592. For instance, one or more cysteines in a hinge region can be deleted or substituted with a different amino acid. The mutated hinge region can comprise no cysteine residues, or it can comprise 1, 2, or 3 fewer cysteine residues than a corresponding, wild-type hinge region. In some embodiments, an Fc region having a mutated hinge region of this type exhibits a reduced ability to dimerize, relative to a wild-type Ig hinge region.

As noted above, HRS-Fc conjugates such as HRS-Fc fusion proteins typically have altered (e.g., improved, increased, decreased) pharmacokinetic properties relative to corresponding HRS polypeptides. Examples of pharmacokinetic properties include stability or half -life, bioavailability (the fraction of a drug that is absorbed), tissue distribution, volume of distribution (apparent volume in which a drug is distributed immediately after it has been injected intravenously and equilibrated between plasma and the surrounding tissues), concentration (initial or steady-state concentration of drug in plasma), elimination rate constant (rate at which drugs are removed from the body), elimination rate (rate of infusion required to balance elimination), area under the curve (AUC or exposure; integral of the concentration-time curve, after a single dose or in steady state), clearance (volume of plasma cleared of the drug per unit time), C _ma x (peak plasma concentration of a drug after oral administration), (time to reach Cmax), Cmin (lowest concentration that a drug reaches before the next dose is administered), and fluctuation (peak trough fluctuation within one dosing interval at steady state). In some aspects, these improved properties are achieved without significantly altering the secondary structure and/or reducing the non-canonical biological activity of the HRS polypeptide. Indeed, some HRS-Fc conjugates have increased non-canonical biological activity.

Hence, in some embodiments, the HRS-Fc conjugate or HRS-Fc fusion polypeptide has a plasma or sera pharmacokinetic AUC profile at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 50, 100, 200, 300, 400, or 500-fold greater than a corresponding unmodified or differently modified HRS polypeptide when administered to a mammal under the same or comparable conditions. In certain embodiments, the HRS-Fc conjugate or HRS-Fc fusion polypeptide has a stability (e.g., as measured by half-life) which is at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 200%, 300%, 400%, or 500% greater than a corresponding unmodified or differently modified HRS polypeptide when compared under similar conditions at room temperature, for example, in PBS at pH 7.4 for about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 days, or 1, 2, 3, 4 weeks or so.

In particular embodiments, a HRS-Fc conjugate or HRS-Fc fusion polypeptide has a biological half life at pH 7.4, 25°C, e.g., a physiological pH, human body temperature (e.g. , in vivo, in serum, in a given tissue, in a given species such as rat, mouse, monkey, or human), of about or at least about 30 minutes, about 1 hour, about 2 hour, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 12 hours, about 18 hours, about 20 hours, about 24 hours, about 30 hours, about 36 hours, about 40 hours, about 48 hours, about 50 hours, about 60 hours, about 70 hours, about 72 hours, about 80 hours, about 84 hours, about 90 hours, about 96 hours, about 120 hours, or about 144 hours or more or any intervening half -life.

In certain embodiments, the HRS-Fc conjugate or HRS-Fc fusion polypeptide has greater bioavailability after subcutaneous (SC) administration compared to a corresponding unmodified HRS- polypeptide. In certain embodiments, the HRS-Fc conjugate or HRS-Fc fusion polypeptide has at least about 20%, at least about 30%, at least about 40%,, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, or at least about 100 %, or more bioavailability compared to the corresponding unmodified HRS polypeptide.

In certain embodiments, the HRS-Fc fusion polypeptide has substantially the same secondary structure as a corresponding unmodified or differently modified HRS polypeptide, as determined via UV circular dichroism analysis. In certain embodiments, the HRS-Fc fusion polypeptide has substantially the same activity of a corresponding unmodified or differently modified HRS polypeptide in an assay of anti-inflammatory activity. In other embodiments, the HRS-Fc fusion polypeptide has greater than 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20-fold the activity of a corresponding unmodified or differently modified HRS polypeptide in an assay of antiinflammatory activity.

In certain embodiments, a peptide linker sequence may be employed to separate the HRS polypeptide(s) and the Fc region(s) or PEG(s) by a distance sufficient to ensure that each polypeptide folds into its desired secondary and tertiary structures. Such a peptide linker sequence can be incorporated into the conjugate or fusion protein using standard techniques well known in the art.

Certain peptide linker sequences may be chosen based on the following exemplary factors: (1) their ability to adopt a flexible extended conformation; (2) their inability to adopt a secondary structure that could interact with functional epitopes on the first and second polypeptides; (3) their physiological stability; and (4) the lack of hydrophobic or charged residues that might react with the polypeptide functional epitopes, or other features. See, e.g., George and Heringa, J Protein Eng. 15:871-879, 2002.

The linker sequence may generally be from 1 to about 200 amino acids in length. Particular linkers can have an overall amino acid length of about 1-200 amino acids, 1-150 amino acids, 1-100 amino acids, 1-90 amino acids, 1-80 amino acids, 1-70 amino acids, 1-60 amino acids, 1-50 amino acids, 1-40 amino acids, 1-30 amino acids, 1-20 amino acids, 1-10 amino acids, 1-5 amino acids, 1-4 amino acids, 1-3 amino acids, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 ,17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90, 100 or more amino acids.

A peptide linker may employ any one or more naturally -occurring amino acids, non-naturally occurring amino acid(s), amino acid analogs, and/or amino acid mimetics as described elsewhere herein and known in the art. Certain amino acid sequences which may be usefully employed as linkers include those disclosed in Maratea et al, Gene 40:39-46, 1985; Murphy et al., PNAS USA. 83:8258- 8262, 1986; U.S. Pat. No. 4,935,233 and U.S. Pat. No. 4,751,180. Particular peptide linker sequences contain Gly, Ser, and/or Asn residues. Other near neutral amino acids, such as Thr and Ala may also be employed in the peptide linker sequence, if desired.

Certain exemplary linkers include Gly, Ser and/or Asn-containing linkers, as follows: [G] _x , [S] _x , [N] _x , [GS] _X , [GGS] _X , [GSS] _X , [GSGS] _X (SEQ ID NO: 173), [GGSG] _X (SEQ ID NO: 174), [GGGS] _X (SEQ ID NO: 175), [GGGGS] _X (SEQ ID NO: 176), [GN] _X , [GGN] _X , [GNN] _X , [GNGN] _X (SEQ ID NO: 177), [GGNG] _X (SEQ ID NO: 178), [GGGN] _X (SEQ ID NO: 179), [GGGGN] _X (SEQ ID NO: 180) linkers, where _x is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 or more. Other combinations of these and related amino acids will be apparent to persons skilled in the art.

Additional examples of linker peptides include, but are not limited to the following amino acid sequences: Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser- (SEQ ID NO: 181); Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly- Gly-Ser-Gly-Gly-Gly- Gly-Ser-(SEQ ID NO: 182); Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser- Gly- Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-(SEQ ID NO: 183); Asp-Ala-Ala-Ala- Lys-Glu-Ala-Ala-Ala-Lys-Asp-Ala- Ala-Ala- Arg-Glu-Ala-Ala-Ala-Arg-Asp-Ala-Ala-Ala-Lys-(SEQ ID NO: 184); and Asn-Val-Asp-His-Lys-Pro-Ser-Asn-Thr-Lys-Val-Asp-Lys-Arg-(SEQ ID NO: 185).

Further non-limiting examples of linker peptides include DGGGS (SEQ ID NO: 186); TGEKP (SEQ ID NO: 187) (see, e.g., Liu et al., PNAS. 94:5525-5530, 1997); GGRR (SEQ ID NO: 188) (Pomerantz et al 1995); (GGGGS) _n (SEQ ID NO: 176) (Kim et al , PNAS. 93: 1156-1160, 1996); EGKSSGSGSESKVD (SEQ ID NO:189) (Chaudhary et al, PNAS. 87: 1066-1070, 1990);

KES GS VS SEQL AQFRSLD (SEQ ID NO: 190) (Bird et al, Science. 242:423-426, 1988),

GGRRGGGS (SEQ ID NO:191); LRQRDGERP (SEQ ID NO: 192); LRQKD GGGSERP (SEQ ID NO: 193); LRQKd(GGGS) ₂ ERP (SEQ ID NO: 194). In specific embodiments, the linker sequence comprises a Gly3 linker sequence, which includes three glycine residues. In particular embodiments, flexible linkers can be rationally designed using a computer program capable of modeling both DNA- binding sites and the peptides themselves (Desjarlais & Berg, PNAS. 90:2256-2260, 1993; and PNAS. 91: 11099-11103, 1994) or by phage display methods.

The peptide linkers may be physiologically stable or may include a releasable linker such as a physiologically degradable or enzymatically cleavable linker (e.g., proteolytically cleavable linker). In certain embodiments, one or more releasable linkers can result in a shorter half-life and more rapid clearance of the conjugate. These and related embodiments can be used, for example, to enhance the solubility and blood circulation lifetime of HRS polypeptides in the bloodstream, while also delivering a HRS polypeptide into the bloodstream that, subsequent to linker degradation, is substantially free of the Fc region(s). These aspects are especially useful in those cases where HRS polypeptides, when permanently conjugated to an Fc region, demonstrate reduced activity. By using the linkers as provided herein, such HRS polypeptides can maintain their therapeutic activity when in conjugated form. As another example, a large and relatively inert HRS-Fc conjugate polypeptide may be administered, which is then degraded in vivo (via the degradable linker) to generate a bioactive HRS polypeptide possessing a portion of the Fc region or lacking the Fc region entirely. In these and other ways, the properties of the HRS-Fc conjugate polypeptide can be more effectively tailored to balance the bioactivity and circulating half-life of the HRS polypeptide over time.

In particular embodiments, the linker peptide comprises an autocatalytic or self-cleaving peptide cleavage site. In a particular embodiment, self-cleaving peptides include those polypeptide sequences obtained from potyvirus and cardiovirus 2A peptides, FMDV (foot-and-mouth disease virus), equine rhinitis A virus, Thosea asigna virus and porcine teschovirus. In certain embodiments, the self-cleaving polypeptide site comprises a 2A or 2A-like site, sequence or domain (Donnelly et ah, J. Gen. Virol. 82: 1027-1041, 2001). Exemplary 2A sites include the following sequences:

LLNFDLLKLAGDVESNPGP (SEQ ID NO: 195); TLNFDLLKLAGDVESNPGP (SEQ ID NO: 196); LLKLAGDVESNPGP (SEQ ID NO: 197); NFDLLKLAGDVESNPGP (SEQ ID NO: 198);

QLLNFDLLKLAGDVESNPGP (SEQ ID NO: 199); APVKQTLNFDLLKLAGDVESNPGP (SEQ ID NO:200); VTELLYRMKRAETYCPRPLLAIHPTEARHKQKIVAPVKQT (SEQ ID NO:201); LNFDLLKLAGDVESNPGP (SEQ ID NO:202);

LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP (SEQ ID NO:203); and

EARHKQKIV APVKQTLNFDLLKLAGDVESNPGP (SEQ ID NO:204). In some embodiments, the autocatalytic peptide cleavage site comprises a translational 2A signal sequence, such as, e.g. , the 2A region of the aphthovirus foot-and-mouth disease virus (FMDV) polyprotein, which is anl8 amino acid sequence. Additional examples of 2A-like sequences that may be used include insectvirus polyproteins, the NS34 protein of type C rotaviruses, and repeated sequences in Trypanosoma spp., as described, for example, in Donnelly et al., Journal of General Virology. 82: 1027-1041, 2001.

Suitable protease cleavages sites and self-cleaving peptides are known to the skilled person {see, e.g., Ryan et ah, J. Gener. Virol. 78:699-722, 1997; and Scymczak et ah, Nature Biotech. 5:589- 594, 2004). Exemplary protease cleavage sites include, but are not limited to the cleavage sites of potyvirus NIa proteases (e.g., tobacco etch virus protease), potyvirus HC proteases, potyvirus PI (P35) proteases, byovirus NIa proteases, byovirus RNA-2 -encoded proteases, aphthovirus L proteases, enterovirus 2 A proteases, rhinovirus 2 A proteases, picorna 3C proteases, comovirus 24K proteases, nepovirus 24K proteases, RTSV (rice tungro spherical virus) 3C-like protease, PYVF (parsnip yellow fleck virus) 3C-like protease, heparin, thrombin, factor Xa and enterokinase. Due to its high cleavage stringency, TEV (tobacco etch virus) protease cleavage sites are included in some embodiments, e.g., EXXYXQ(G/S) (SEQ ID NO:205), for example, ENLYFQG (SEQ ID NO:206) and ENLYFQS (SEQ ID NO:207), wherein X represents any amino acid (cleavage by TEV occurs between Q and G or Q and S).

Further examples of enzymatically degradable linkers suitable for use in particular embodiments include, but are not limited to: an amino acid sequence cleaved by a serine protease such as thrombin, chymotrypsin, trypsin, elastase, kallikrein, or substilisin. Illustrative examples of thrombin-cleavable amino acid sequences include, but are not limited to: -Gly-Arg-Gly-Asp-(SEQ ID NO:208), -Gly-Gly-Arg-, -Gly- Arg-Gly-Asp-Asn-Pro-(SEQ ID NO:209), -Gly-Arg-Gly-Asp-Ser- (SEQ ID NO:210), -Gly-Arg-Gly-Asp-Ser-Pro-Lys-(SEQ ID NO:211), -Gly -Pro- Arg-, -Val-Pro-Arg- , and -Phe- Val -Arg-. Illustrative examples of elastase-cleavable amino acid sequences include, but are not limited to: -Ala-Ala-Ala-, -Ala-Ala-Pro- Val-(SEQ ID NO:212), -Ala-Ala-Pro-Leu-(SEQ ID NO:213), -Ala-Ala-Pro-Phe-(SEQ ID NO:214), -Ala-Ala-Pro-Ala-(SEQ ID NO:215), and -Ala-Tyr- Leu-Val-(SEQ ID NO:216).

Enzymatically degradable linkers also include amino acid sequences that can be cleaved by a matrix metalloproteinase such as collagenase, stromelysin, and gelatinase. Illustrative examples of matrix metalloproteinase-cleavable amino acid sequences include, but are not limited to: -Gly-Pro-Y- Gly-Pro-Z-(SEQ ID NO:217), -Gly-Pro-, Leu-Gly-Pro-Z-(SEQ ID NO:218), -Gly-Pro-Ile-Gly-Pro-Z- (SEQ ID NO:219), and -Ala-Pro-Gly-Leu-Z-(SEQ ID NO:220), where Y and Z are amino acids. Illustrative examples of collagenase-cleavable amino acid sequences include, but are not limited to: - Pro-Leu-Gly-Pro-D-Arg-Z-(SEQ ID NO:221), -Pro- Leu-Gly-Leu-Leu-Gly-Z-(SEQ ID NO:222), - Pro-Gln-Gly-Ile-Ala-Gly-Trp-(SEQ ID NO:223), -Pro-Leu-Gly-Cys(Me)-His-(SEQ ID NO:224), - Pro-Leu-Gly-Leu-Tyr-Ala-(SEQ ID NO:225), -Pro-Leu-Ala-Leu-Tφ-Ala-Arg-(SEQ ID NO:226), and -Pro-Leu-Ala-Tyr-Tφ-Ala-Arg-(SEQ ID NO:227), where Z is an amino acid. An illustrative example of a stromelysin-cleavable amino acid sequence is -Pro-Tyr-Ala-Tyr-Tyr-Met-Arg-(SEQ ID NO:228); and an example of a gelatinase-cleavable amino acid sequence is -Pro-Leu-Gly-Met-Tyr- Ser-Arg-(SEQ ID NO:229).

Enzymatically degradable linkers suitable for use in particular embodiments include amino acid sequences that can be cleaved by an angiotensin converting enzyme, such as, for example, -Asp- Lys-Pro-, -Gly-Asp-Lys-Pro-(SEQ ID NO:230), and -Gly-Ser-Asp-Lys-Pro-(SEQ ID NO:231).

Enzymatically degradable linkers suitable for use in particular embodiments include amino acid sequences that can be degraded by cathepsin B, such as, for example, Val-Cit, Ala-Leu-Ala-Leu- (SEQ ID NO:232), Gly-Phe-Leu-Gly-(SEQ ID NO:233) and Phe-Lys.

In particular embodiments, a releasable linker has a half life at pH 7.4, 25°C, e.g., a physiological pH, human body temperature (e.g., in vivo, in serum, in a given tissue), of about 30 minutes, about 1 hour, about 2 hour, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 12 hours, about 18 hours, about 24 hours, about 36 hours, about 48 hours, about 72 hours, or about 96 hours or more or any intervening half -life. One having skill in the art would appreciate that the half life of a HRS-Fc conjugate polypeptide can be finely tailored by using a particular releasable linker.

In certain embodiments, however, any one or more of the peptide linkers are optional. For instance, linker sequences may not required when the first and second polypeptides have non-essential N-terminal and/or C-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference.

The HRS polypeptides and polynucleotides, for example, expressible polynucleotides, can be used in any of the compositions, methods, and/or kits described herein.

Immunomodulatory Agents

Certain embodiments employ one or more immunomodulatory agents. Exemplary immunomodulatory agents include small molecules, polypeptides, for example, antibodies and antigen-binding fragments thereof, ligands, small peptides, antisense agents, RNAi agents, and mixtures thereof.

In some embodiments, the immunomodulatory agent is selected from one or more of a sphingosine-1 -phosphate (SIP) and/or SIP receptor (S1PR) modulator, a steroid, a calcineurin inhibitor, a mechanistic target of rapamycin (mTOR) inhibitor, an indoleamine-pyrrole 2,3- dioxygenase (IDO) inhibitor, an inosine-5' -monophosphate dehydrogenase (DVIPDH) inhibitor, a cytokine and/or cytokine receptor inhibitor, a B cell receptor inhibitor, a kinase inhibitor, and a cytostatic agent such as methotrexate.

In some embodiments, the immunomodulatory agent is pirfenidone, which is often used for the treatment of idiopathic pulmonary fibrosis (IPF). Pirfenidone has antifibrotic and antiinflammatory properties in various in vitro systems and animal models of fibrosis. For example, cell- based studies have shown that pirfenidone reduces fibroblast proliferation, inhibits TGF-β stimulated collagen production, and reduces the production of fibrogenic mediators such as TGF-β. Pirfenidone has also been shown to reduce production of inflammatory mediators such as TNF-a and IL-Ιβ in both cultured cells and isolated human peripheral blood mononuclear cells. In the United States, pirfenidone is approved for the treatment of IPF as an oral 801 mg dosage unit (three 267 mg capsules) taken orally 3xday, for a total oral dosage of 2403 mg/day. Additional exemplary dosages of pirfenidone are described herein.

In some embodiments, the immunomodulatory agent is nintedanib, which is also used for the treatment of IPF. Nintedanib inhibits certain growth factor receptors involved in pulmonary fibrosis, including platelet-derived growth factor receptor (PDGFR), fibroblast growth factor receptor (FGFR) and vascular endothelial growth factor receptor (VEGFR). It is believed that nintedanib reduces disease progression in IPF and slows the decline in lung function by blocking the signaling pathways that are involved in fibrotic processes. Nintedanib is formulated as salt with ethanesulfonic acid. In the United States, nintedanib is approved for the treatment of IPF as an oral 150 mg dosage unit taken 2xday for a total of 300 mg/day, which can be reduced for adverse effects to about 100 mg dosage taken 2xday for total of 200 mg/day. Additional exemplary dosages of nintedanib are described herein.

In some embodiments, the immunomodulatory agent is a sphingosine-1 -phosphate (SIP) and/or a SIP receptor (S1PR) modulator. General examples of modulators include SIP and/or S1PR antagonists or inhibitors, and SIP and/or S 1PR agonists or activators. S IP is a bioactive lipid with diverse biological functions, including cell proliferation, differentiation, angiogenesis, chemotaxis, and migration. Many of the activities of SIP are mediated through five closely related G-protein- coupled receptors of the sphingosine-1 -phosphate receptor family (S1PR) which play a crucial role in sphingolipid metabolism. The SIPRs include SIPRi, S 1PR ₂ , SIPR3, SIPR4, and S1PR ₅ . The expression of these receptors varies: SIPRi, SIPR2, and S IPR3 are expressed in a wide variety of cell types but mostly heavily on leukocytes, SIPR4 is expressed mostly in lymphoid and hematopoietic tissues, and S IPR5 is expressed mainly in the spleen and the white matter of the central nervous system (CNS).

Specific non-limiting examples of SIP or S1PR modulators include amiselimod (also MT- 1303; a S1PR antagonist; see Kappos et al., Lancet Neurol 2016; 15: 1148-59, 2016), fingolimod (SIPRi functional antagonist), sonepcizumab (S IP-specific monoclonal antibody), KRP203 (SIPRi agonist), SEW2871 (SIPRi agonist), siponimod (SIPRi and S1PR ₅ modulator), RPC1063 (SIPRi modulator), ONO-4641 (SIPRi and S 1PR ₅ agonist), JTE-013 (S1PR ₂ antagonist), GSK2018682 (SIPRi agonist), ponesimod (SIPRi agonist), suramin (selective S IPR3 and SIPR5 antagonist), VPC23019 (aryl-amide analogs; competitive SIPRi and S IPR3 antagonists); and W146 (selective SIPRi antagonist). In specific embodiments, the SIP or S1PR modulator is amiselimod.

Certain SIP or S1PR modulators include an antibody or antigen-binding fragment or small molecule that specifically binds to SIP or an S1PR (see, e.g., sonepcizumab, which binds to SIP). In some embodiments, the antibody or antigen-binding fragment thereof is an SIP and/or S1PR antagonist. In certain embodiments, the antibody or antigen-binding fragment thereof is an S IP and/or S1PR agonist.

Certain S1PR antagonists or inhibitors include antisense agents and RNAi agents that are directed against an S1PR coding sequence (see, e.g., Accession Nos. NM 001400.4; NM 004230.3). Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes an S 1PR, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes an S1PR, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes an S1PR.

In some embodiments, the immunomodulatory agent is a steroid or corticosteroid, for example, a glucocorticoid. In particular embodiments, the steroid is an anti-inflammatory steroid. Examples of steroids include betamethasone, budesonide, Cortisol (hydrocortisone), cortisone, deflazacort, deoxycorticosterone, dexamethasone, fludrocortisone, hydrocortisone,

methylprednisolone, prednisone, prednisolone, and triamcinolone, among others.

In some embodiments, the immunomodulatory agent is a calcineurin antagonist or inhibitor. Calcineurin is a calcium and calmodulin dependent serine/threonine protein phosphatase, which activates the T cells. Specifically, calcineurin activates nuclear factor of activated T cell (NFATc), which translocates into the nucleus to upregulate the expression of interleukin 2 (IL-2), which then stimulates the growth and differentiation of T cell responses. Specific examples of calcineurin antagonists or inhibitors include cyclosporin, pimecrolimus, and tacrolimus.

Certain calcineurin antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to calcineurin. Also included are antisense agents and RNAi agents that are directed against a calcineurin coding sequence, or a subunit thereof (see, e.g., Accession Nos. NM 000944; NM 021132; NM 005605; NM 000945; NM 147180). Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes calcineurin or a subunit thereof, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes calcineurin or a subunit thereof, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes calcineurin or a subunit thereof.

In some embodiments, the immunomodulatory agent is a mechanistic target of rapamycin (mTOR) antagonist or inhibitor. mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases, and is the catalytic subunit of two structurally distinct complexes: mTORCl and mTORC2, which localize to different subcellular compartments, thus affecting their activation and function particular. As a core component of both complexes, mTOR functions as a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, autophagy, and transcription. As a core component of mTORC2, mTOR also functions as a tyrosine protein kinase that promotes the activation of insulin receptors and insulin-like growth factor 1 receptors. mTORC2 has also been implicated in the control and maintenance of the actin cytoskeleton. mTOR plays a role in fibrotic diseases and autoimmunity, and blockade of the mTORC pathway is under investigation as a treatment for such diseases.

Particular examples of mTOR inhibitors include everolimus, rapamycin, deforolimus, and temsirolimus. General examples of mTOR inhibitors include ATP -competitive mTOR kinase inhibitors, including mTORC l/mTORC2 dual inhibitors, and mTOR/PI3K dual inhibitors, which inhibit mTORCl, mTORC2, and the catalytic isoforms of PI3K. Specific examples include dactolisib, BGT226, SF1126, PKI-587, NVPBE235, sapanisertib, AZD8055, and AZD2014.

Certain mTOR antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to mTOR or a member of an mTOR complex. Also included are antisense agents and RNAi agents that are directed against a coding sequence of mTOR or a member of an mTOR complex (see, e.g., Ravichandran et al., Hum Mol Genet. 23:4919-31, 2014). Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes mTOR or a member of an mTOR complex, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3 ' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes mTOR or a member of an mTOR complex, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes mTOR or a member of an mTOR complex.

In some embodiments, the immunomodulatory agent is an IDO antagonist or inhibitor. IDO is a tryptophan catabolic enzyme with immune-inhibitory properties. For example, IDO is known to suppress T-cells and NK cells, generate and activate Tregs and myeloid-derived suppressor cells, and promote tumor angiogenesis. Specific examples of IDO antagonists or inhibitors include indoximod (NLG-8189), 1-methyl-tryptophan (1MT), β-Carboline (norharmane; 9H-pyrido[3,4-b]indole), rosmarinic acid, and epacadostat (see, e.g., Sheridan, Nature Biotechnology. 33:321-322, 2015). Certain IDO antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to IDO (see, e.g., Platten et al., Front Immunol. 5: 673, 2014). Also included are antisense agents and RNAi agents that are directed against an IDO coding sequence (see, e.g., Accession No. AH002828.2). Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes IDO, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3 ' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes IDO, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes IDO.

In some embodiments, the immunomodulatory agent is an inosine-5'-monophosphate dehydrogenase (DVIPDH) antagonist or inhibitor. IMPDH is a purine biosynthetic enzyme that catalyzes the nicotinamide adenine dinucleotide (NAD+)-dependent oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), the first committed and rate-limiting step towards the de novo biosynthesis of guanine nucleotides from IMP. Guanine nucleotide synthesis is essential for maintaining normal cell function and growth, and is also important for the maintenance of cell proliferation and immune responses. In particular, B and T cells display a dependence on IMPDH for normal activation and function, and demonstrate upregulated IMPDH expression.

Specific examples of IMPDH inhibitors include mycophenolic acid (mycophenolate mofetil), ribavirin, and 6TGMP (6-thioguanine monophosphate). Certain IMPDH antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to IMPDH. Also included are antisense agents and RNAi agents that are directed against an IMPDH coding sequence. Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes IMPDH, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3 ' or 5 ' splice site of a pre-processed mRNA, a branch point, a 3 ' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes IMPDH, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes IMPDH.

In some embodiments, the immunomodulatory agent is a cytokine and/or cytokine receptor antagonist or inhibitor. Cytokines are small (glyco)proteins (with molecular weights of 8-75 kDa) which play a role in hematopoiesis, immune reactions and inflammation. Certain exemplary cytokine inhibitors decrease the synthesis of cytokines, decrease the concentration of cytokines in free active form, block the interaction between cytokines and their cognate receptors, and/or interfere with the signaling of cytokine receptors.

In some embodiments, the target cytokine or cytokine receptor is an inflammatory or proinflammatory cytokine or cytokine receptor. Examples of target cytokines include, without limitation, interleukin-1 (IL-1) including IL- la and IL-Ιβ, interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin- 8 (IL-8), interleukin-11 (IL-11), interleukin-12 (IL-12), interleukin-17 (IL-17), interleukin-18 (IL-18), interleukin-20 (IL-20), interleukin-33 (IL-33), tumor necrosis factor (TNF), interferon gamma (IFN- gamma), transforming growth factor-β (TGF-β), and granulocyte-macrophage colony stimulating factor (GM-CSF), and their cognate cytokine receptors, for example, IL-1R, IL-6R, 1L-8R, IL-11R, IL-12R, IL-17R, IL-18R, IL-20R, ST2 (Interleukin 1 receptor-like 1, IL1RL1), a TNFR such as TNFR1, interferon-gamma receptor (IFNGR), a TGF-β receptor such as TGFβRl (ALK5) or TGFβR2.

Specific examples of cytokine and/or cytokine receptor inhibitors include TNF -alpha inhibitors such as etanercept, which is a recombinant fusion protein of the soluble type II TNF receptor on a human IgGl backbone, and infliximab, which is a chimeric anti-TNF-alpha monoclonal antibody containing a murine TNF-alpha binding region and human IgGl backbone. Also included as TNF inhibitors are adalimumab, certolizumab, and golimumab.

Particular examples of interleukin inhibitors include, for example, IL-1R antagonists such as anakinra, IL-1 inhibitors such as rilonacept (a dimeric fusion protein consisting of the ligand-binding domains of the extracellular portions of the IL-1R1 component and IL-1 receptor accessory protein (IL-lRAcP) linked in-line to the fragment-cry stallizable portion (Fc region) of human IgGl that binds and neutralizes IL-1), IL-2 competitive inhibitors such as basiliximab (a chimeric mouse-human monoclonal antibody to the a chain (CD25) of the IL-2 receptor of T cells) and daclizumab (a humanized monoclonal antibody that binds to CD25), IL-^-specific inhibitors such as canakinumab (a human monoclonal antibody), IL-17 antagonists such as ixekizumab (ahumanized monoclonal antibody that binds to IL-17) and secukinumab (a human IgGlK monoclonal antibody that binds to the protein interleukin (IL)-17A), IL-5 inhibitors such as mepolizumab (a humanized monoclonal antibody that binds to IL-5 and prevents it from binding to the alpha subunit of the IL-5 receptor) and reslizumab, IL-6 inhibitors such as siltuximab (an antibody that binds to IL-6), sirukumab (an antibody that binds to IL-6), serilumab (an antibody that binds to the IL-6 receptor), an tocilizumab (an antibody that binds to the IL-6 receptor), and IL-12/IL-23 signaling inhibitors such as ustekinumab (an antibody that binds to the p-40 subunit of both IL-12 and IL-23).

Certain cytokine and/or cytokine receptor antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to the cytokine and/or cytokine receptor, for example, one or more of the foregoing cytokines and/or cytokine receptors. Also included are antisense agents and RNAi agents that are directed against a cytokine and/or cytokine receptor coding sequence, for example, one or more of the foregoing cytokines and/or cytokine receptors. Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes a cytokine or a cytokine receptor, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3 ' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes a cytokine or a cytokine receptor, and optionally an antisense strand that is complementary or substantially complementary to the mRNA target sequence that encodes a cytokine or a cytokine receptor.

In some embodiments, the immunomodulatory agent is a kinase antagonist or inhibitor, that is, an inhibitor that targets or is targeted against one or more kinases. General examples include tyrosine kinase inhibitors (TKIs). Examples of target kinases include, without limitation, Janus kinase (JAK, including JAK1, JAK2, JAK3, TYK2), epidermal growth factor receptor (EGFR), Receptor tyrosine-protein kinase erbB-2 (Her2/neu, or ERBB2), Bcr-Abl, c-SRC, Mitogen-activated protein kinase (MAP) kinase, anaplastic lymphoma kinase (ALK), spleen tyrosine kinase (SYK), Bruton's tyrosine kinase (BTK), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor (VEGFR, including VEGFR1, VEGFR2, VEGFR3), a fibroblast growth factor receptor (FGFR), B-Raf, RET proto-oncogene, platelet-derived growth factor receptors (PDGF-R), tropomyosin receptor kinase (Trk, including TrkA, TrkB, TrkC), and c-Met, among others. Thus, in certain embodiments, the kinase inhibitor is an inhibitor or antagonist of one or more of the foregoing kinases.

Specific examples of kinase inhibitors include JAK inhibitors such as baricitinib, fedratinib, filgotinib, gandotinib, lestaurtinib, momelotinib, pacritinib, peficitinib, ruxolitinib, tofacitinib, and padacitinib. Additional examples of kinase inhibitors include, without limitation, nintedanib, afatinib, axitinib, bosutinib, cetuximab, cobimetinib, crizotinib, cabozantinib, dasatinib, entrectinib, erlotinib, fostamatinib, gefitinib, ibrutinib, imatinib, lapatinib, lenvatinib, mubritinib, neratinib, nilotinib, pazopanib, pegaptanib, sorafenib, sunitinib, SU6656, toceranib, vandetanib, vatalanib, and vemurafenib.

Certain kinase antagonists or inhibitors include an antibody or antigen-binding fragment or small molecule that specifically binds to the kinase, for example, one or more of the foregoing kinases. Also included are antisense agents and RNAi agents that are directed against a kinase coding sequence, for example, one or more of the foregoing kinases. Certain antisense agents specifically hybridizes to a target region within a pre-mRNA or mRNA target sequence that encodes a kinase, for example, wherein the target region is selected from one or more of an AUG start codon of the mRNA, a region upstream of the AUG start codon, a region downstream of the AUG codon, a 3' or 5' splice site of a pre-processed mRNA, a branch point, a 3 ' untranslated region (UTR), and a polyadenylation signal sequence. Certain RNAi agents comprise a sense strand that is substantially identical to an mRNA target sequence that encodes a kinase, and optionally an antisense strand that is

complementary or substantially complementary to the mRNA target sequence that encodes the kinase.

In some embodiments, the immunomodulatory agent is a B cell receptor inhibitor, for example, an agent that is targeted against CD20. B lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. The protein has no known natural ligand, and its function is to enable optimal B cell immune response, specifically against T-independent antigens. Exemplary immunomodulatory agents directed against CD20 include the monoclonal antibodies ibritumomab tiuxetan, obinutuzumab, ocaratuzumab, ocrelizumab, rituximab, tositumomab, and veltuzumab.

In some embodiments, the immunomodulatory agent is a cytostatic or cytotoxic agent.

Examples of cytostatic or cytotoxic agents include azathioprine, chlorambucil, cyclophosphamide, cyclosporin A, methotrexate, and nitrogen mustard, among others.

In some embodiments, as noted above, the immunomodulatory agent is a "small molecule," which refers to an organic compound that is of synthetic or biological origin (biomolecule), but is typically not a polymer. Organic compounds refer to a large class of chemical compounds whose molecules contain carbon, typically excluding those that contain only carbonates, simple oxides of carbon, or cyanides. A "biomolecule" refers generally to an organic molecule that is produced by a living organism, including large polymeric molecules (biopolymers) such as peptides,

polysaccharides, and nucleic acids as well, and small molecules such as primary secondary metabolites, lipids, phospholipids, glycolipids, sterols, glycerolipids, vitamins, and hormones. A "polymer" refers generally to a large molecule or macromolecule composed of repeating structural units, which are typically connected by covalent chemical bond. In certain embodiments, a small molecule has a molecular weight of about or less than about 1000-2000 Daltons, typically between about 300 and 700 Daltons, and including about or less than about 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 500, 650, 600, 750, 700, 850, 800, 950, 1000 or 2000 Daltons.

Certain small molecules can have the "specific binding" characteristics described herein. For instance, in some embodiments a small molecule specifically binds to a target (for example, SIP, SIPR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, a kinase) with a binding affinity (Kd) of about, at least about, or less than about, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 40, or 50 nM.

In particular embodiments, the immunomodulatory agent is a polypeptide or peptide. The terms "peptide" and "polypeptide" are used interchangeably herein, however, in certain instances, the term "peptide" can refer to shorter polypeptides, for example, polypeptides that consist of about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, or 50 amino acids, including all integers and ranges (e.g., 5-10, 8-12, 10-15) in between. Polypeptides and peptides can be composed of naturally -occurring amino acids and/or non-naturally occurring amino acids, as described herein. Antibodies are also included as polypeptides.

The binding properties of polypeptides can be quantified using methods well known in the art (see Davies et al., Annual Rev. Biochem. 59:439-473, 1990). In some embodiments, a polypeptide specifically binds to a target molecule (for example, SIP, SIPR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, a kinase or an epitope thereof ) with an equilibrium dissociation constant that is about or ranges from about <10-7 to about 10-8 M. In some embodiments, the equilibrium dissociation constant is about or ranges from about <10-9 M to about <10-10 M. In certain illustrative embodiments, the polypeptide has an affinity (Kd) for a target described herein (to which it specifically binds, including, for example, SIP, SIPR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, or a kinase) of about, at least about, or less than about, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 40, or 50 nM.

In some embodiments, the immunomodulatory agent is an antibody or "antigen-binding fragment thereof that specifically binds to a target described herein. The antibody or antigen-binding fragment can be of essentially any type. As is well known in the art, an antibody is an

immunoglobulin molecule capable of specific binding to a target (for example, SIP, SIPR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, a kinase), through at least one epitope recognition site, located in the variable region of the immunoglobulin molecule.

As used herein, the term "antibody" encompasses not only intact polyclonal or monoclonal antibodies, but also fragments thereof (such as dAb, Fab, Fab', F(ab')2, Fv), single chain (ScFv), synthetic variants thereof, naturally occurring variants, fusion proteins comprising an antibody portion with an antigen-binding fragment of the required specificity, humanized antibodies, chimeric antibodies, and any other modified configuration of the immunoglobulin molecule that comprises an antigen-binding site or fragment (epitope recognition site) of the required specificity. Certain features and characteristics of antibodies (and antigen-binding fragments thereof) are described in greater detail herein.

The term "antigen-binding fragment" as used herein refers to a polypeptide fragment that contains at least one CDR of an immunoglobulin heavy and/or light chain that binds to the antigen of interest. In this regard, an antigen-binding fragment of the herein described antibodies may comprise 1, 2, 3, 4, 5, or all 6 CDRs of a VH and VL sequence from antibodies that bind to a target molecule.

The term "antigen" refers to a molecule or a portion of a molecule capable of being bound by a selective binding agent, such as an antibody, and additionally capable of being used in an animal to produce antibodies capable of binding to an epitope of that antigen. An antigen may have one or more epitopes.

The term "epitope" includes any determinant, for example, a polypeptide determinant, capable of specific binding to an immunoglobulin or T-cell receptor. An epitope is a region of an antigen or target protein that is bound by an antibody. In certain embodiments, epitope determinants include chemically active surface groupings of molecules such as amino acids, sugar side chains, phosphoryl or sulfonyl, and may in certain embodiments have specific three-dimensional structural characteristics, and/or specific charge characteristics. Epitopes can be contiguous or non-contiguous in relation to the primary structure of the antigen.

A molecule such as a polypeptide or antibody is said to exhibit "specific binding" or "preferential binding" if it reacts or associates more frequently, more rapidly, with greater duration and/or with greater affinity with a particular cell or substance than it does with alternative cells or substances. An antibody "specifically binds" or "preferentially binds" to a target if it binds with greater affinity, avidity, more readily, and/or with greater duration than it binds to other substances, for example, by a statistically significant amount. For instance, an antibody that specifically or preferentially binds to a specific epitope is an antibody that binds that specific epitope with greater affinity, avidity, more readily, and/or with greater duration than it binds to other epitopes. It is also understood by reading this definition that, for example, an antibody (or moiety or epitope) that specifically or preferentially binds to a first target may or may not specifically or preferentially bind to a second target. As such, "specific binding" or "preferential binding" does not necessarily require (although it can include) exclusive binding. Generally, but not necessarily, reference to binding means preferential binding.

Immunological binding generally refers to the non-covalent interactions of the type which occur between an immunoglobulin molecule and an antigen for which the immunoglobulin is specific, for example by way of illustration and not limitation, as a result of electrostatic, ionic, hydrophilic and/or hydrophobic attractions or repulsion, steric forces, hydrogen bonding, van der Waals forces, and other interactions. The strength, or affinity of immunological binding interactions can be expressed in terms of the dissociation constant (Kd) of the interaction, wherein a smaller Kd represents a greater affinity. Immunological binding properties of selected polypeptides can be quantified using methods well known in the art. One such method entails measuring the rates of antigen-binding site/antigen complex formation and dissociation, wherein those rates depend on the concentrations of the complex partners, the affinity of the interaction, and on geometric parameters that equally influence the rate in both directions. Thus, both the "on rate constant" (K _on ) and the "off rate constant" (Kos) can be determined by calculation of the concentrations and the actual rates of association and dissociation. The ratio of K ₀ ff/ _on enables cancellation of all parameters not related to affinity, and is thus equal to the dissociation constant Kd.

Antibodies may be prepared by any of a variety of techniques known to those of ordinary skill in the art. See, e.g., Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 1988. Monoclonal antibodies specific for a polypeptide of interest may be prepared, for example, using the technique of Kohler and Milstein, Eur. J. Immunol. 6:511-519, 1976, and improvements thereto. Also included are methods that utilize transgenic animals such as mice to express human antibodies. See, e.g., Neuberger et al., Nature Biotechnology 14:826, 1996; Lonberg et al., Handbook of Experimental Pharmacology 113 :49-101, 1994; and Lonberg et al., Internal Review of Immunology 13 :65-93, 1995. Particular examples include the VELOCIMMUNE® platform by REGENEREX® (see, e.g., U.S. Patent No. 6,596,541).

Antibodies can also be generated or identified by the use of phage display or yeast display libraries (see, e.g., U.S. Patent No. 7,244,592; Chao et al., Nature Protocols. 1:755-768, 2006). Non- limiting examples of available libraries include cloned or synthetic libraries, such as the Human Combinatorial Antibody Library (HuCAL), in which the structural diversity of the human antibody repertoire is represented by seven heavy chain and seven light chain variable region genes. The combination of these genes gives rise to 49 frameworks in the master library. By superimposing highly variable genetic cassettes (CDRs = complementarity determining regions) on these frameworks, the vast human antibody repertoire can be reproduced. Also included are human libraries designed with human-donor-sourced fragments encoding a light-chain variable region, a heavy -chain CDR-3, synthetic DNA encoding diversity in heavy -chain CDR-1, and synthetic DNA encoding diversity in heavy -chain CDR-2. Other libraries suitable for use will be apparent to persons skilled in the art.

In certain embodiments, antibodies and antigen-binding fragments thereof as described herein include a heavy chain and a light chain CDR set, respectively interposed between a heavy chain and a light chain framework region (FR) set which provide support to the CDRs and define the spatial relationship of the CDRs relative to each other. As used herein, the term "CDR set" refers to the three hypervariable regions of a heavy or light chain V region. Proceeding from the N-terminus of a heavy or light chain, these regions are denoted as "CDR1," "CDR2," and "CDR3" respectively. An antigen- binding site, therefore, includes six CDRs, comprising the CDR set from each of a heavy and a light chain V region. A polypeptide comprising a single CDR, (e.g., a CDR1, CDR2 or CDR3) is referred to herein as a "molecular recognition unit." Crystallographic analysis of a number of antigen-antibody complexes has demonstrated that the amino acid residues of CDRs form extensive contact with bound antigen, wherein the most extensive antigen contact is with the heavy chain CDR3. Thus, the molecular recognition units are primarily responsible for the specificity of an antigen-binding site.

As used herein, the term "FR set" refers to the four flanking amino acid sequences which frame the CDRs of a CDR set of a heavy or light chain V region. Some FR residues may contact bound antigen; however, FRs are primarily responsible for folding the V region into the antigen- binding site, particularly the FR residues directly adjacent to the CDRs. Within FRs, certain amino residues and certain structural features are very highly conserved. In this regard, all V region sequences contain an internal disulfide loop of around 90 amino acid residues. When the V regions fold into a binding-site, the CDRs are displayed as projecting loop motifs which form an antigen- binding surface. It is generally recognized that there are conserved structural regions of FRs which influence the folded shape of the CDR loops into certain "canonical" structures— regardless of the precise CDR amino acid sequence. Further, certain FR residues are known to participate in non- covalent interdomain contacts which stabilize the interaction of the antibody heavy and light chains.

The structures and locations of immunoglobulin variable domains may be determined by reference to Kabat, E. A. et al., Sequences of Proteins of Immunological Interest. 4th Edition. US Department of Health and Human Services. 1987, and updates thereof.

Also include are "monoclonal" antibodies, which refer to a homogeneous antibody population wherein the monoclonal antibody is comprised of amino acids (naturally occurring and non-naturally occurring) that are involved in the selective binding of an epitope. Monoclonal antibodies are highly specific, being directed against a single epitope. The term "monoclonal antibody" encompasses not only intact monoclonal antibodies and full-length monoclonal antibodies, but also fragments thereof (such as Fab, Fab', F(ab')2, Fv), single chain (ScFv), variants thereof, fusion proteins comprising an antigen-binding portion, humanized monoclonal antibodies, chimeric monoclonal antibodies, and any other modified configuration of the immunoglobulin molecule that comprises an antigen-binding fragment (epitope recognition site) of the required specificity and the ability to bind to an epitope. It is not intended to be limited as regards the source of the antibody or the manner in which it is made (e.g., by hybridoma, phage selection, recombinant expression, transgenic animals). The term includes whole immunoglobulins as well as the fragments etc. described above under the definition of "antibody."

The proteolytic enzyme papain preferentially cleaves IgG molecules to yield several fragments, two of which (the F(ab) fragments) each comprise a covalent heterodimer that includes an intact antigen-binding site. The enzyme pepsin is able to cleave IgG molecules to provide several fragments, including the F(ab')2 fragment which comprises both antigen-binding sites. An Fv fragment for use according to certain embodiments of the present invention can be produced by preferential proteolytic cleavage of an IgM, and on rare occasions of an IgG or IgA immunoglobulin molecule. Fv fragments are, however, more commonly derived using recombinant techniques known in the art. The Fv fragment includes a non-covalent VH::VL heterodimer including an antigen-binding site which retains much of the antigen recognition and binding capabilities of the native antibody molecule. See Inbar et al., PNAS USA. 69:2659-2662, 1972; Hochman et al., Biochem. 15:2706- 2710, 1976; and Ehrlich et al., Biochem. 19:4091-4096, 1980.

In certain embodiments, single chain Fv or scFV antibodies are contemplated. For example, Kappa bodies (111 et al., Prot. Eng. 10:949-57, 1997); minibodies (Martin et al., EMBO J 13:5305-9, 1994); diabodies (Holliger et al., PNAS 90: 6444-8, 1993); or Janusins (Traunecker et al., EMBO J 10: 3655-59, 1991; and Traunecker et al., Int. J. Cancer Suppl. 7:51-52, 1992), may be prepared using standard molecular biology techniques following the teachings of the present application with regard to selecting antibodies having the desired specificity.

A single chain Fv (sFv) polypeptide is a covalently linked VH::VL heterodimer which is expressed from a gene fusion including VH- and VL-encoding genes linked by a peptide-encoding linker. Huston et al. (PNAS USA. 85(16):5879-5883, 1988). A number of methods have been described to discern chemical structures for converting the naturally aggregated— but chemically separated— light and heavy polypeptide chains from an antibody V region into an sFv molecule which will fold into a three dimensional structure substantially similar to the structure of an antigen-binding site. See, e.g., U.S. Pat. Nos. 5,091,513 and 5,132,405, to Huston et al.; and U.S. Pat. No. 4,946,778, to Ladner et al.

In certain embodiments, an antibody as described herein is in the form of a "diabody." Diabodies are multimers of polypeptides, each polypeptide comprising a first domain comprising a binding region of an immunoglobulin light chain and a second domain comprising a binding region of an immunoglobulin heavy chain, the two domains being linked (e.g. by a peptide linker) but unable to associate with each other to form an antigen binding site: antigen binding sites are formed by the association of the first domain of one polypeptide within the multimer with the second domain of another polypeptide within the multimer (WO94/13804). A dAb fragment of an antibody consists of a VH domain (Ward et al., Nature 341:544-546, 1989). Diabodies and other multivalent or multispecific fragments can be constructed, for example, by gene fusion (see WO94/13804; and Holliger et al., PNAS USA. 90:6444-6448, 1993)).

Minibodies comprising a scFv joined to a CH3 domain are also included (see Hu et al., Cancer Res. 56:3055-3061, 1996). See also Ward et al., Nature. 341:544-546, 1989; Bird et al., Science. 242:423-426, 1988; Huston et al., PNAS USA. 85:5879-5883, 1988); PCT/US92/09965; WO94/13804; and Reiter et al., Nature Biotech. 14: 1239-1245, 1996. Where bispecific antibodies are to be used, these may be conventional bispecific antibodies, which can be manufactured in a variety of ways (Holliger and Winter, Current Opinion Biotechnol. 4:446-449, 1993), e.g. prepared chemically or from hybrid hybridomas, or may be any of the bispecific antibody fragments mentioned above. Diabodies and scFv can be constructed without an Fc region, using only variable domains, potentially reducing the effects of anti-idiotypic reaction.

Bispecific diabodies, as opposed to bispecific whole antibodies, may also be particularly useful because they can be readily constructed and expressed in E. coli. Diabodies (and many other polypeptides such as antibody fragments) of appropriate binding specificities can be readily selected using phage display (WO94/13804) from libraries. If one arm of the diabody is to be kept constant, for instance, with a specificity directed against antigen X, then a library can be made where the other arm is varied and an antibody of appropriate specificity selected. Bispecific whole antibodies may be made by knobs-into-holes engineering (Ridgeway et al., Protein Eng., 9:616-621, 1996).

In certain embodiments, the antibodies described herein may be provided in the form of a UniBody®. A UniBody® is an IgG4 antibody with the hinge region removed (see GenMab Utrecht, The Netherlands; see also, e.g., US20090226421). This antibody technology creates a stable, smaller antibody format with an anticipated longer therapeutic window than current small antibody formats. IgG4 antibodies are considered inert and thus do not interact with the immune system. Fully human IgG4 antibodies may be modified by eliminating the hinge region of the antibody to obtain half- molecule fragments having distinct stability properties relative to the corresponding intact IgG4 (GenMab, Utrecht). Halving the IgG4 molecule leaves only one area on the UniBody® that can bind to cognate antigens (e.g., disease targets) and the UniBody® therefore binds univalently to only one site on target cells.

In certain embodiments, the antibodies provided herein may take the form of a nanobody. Minibodies are encoded by single genes and are efficiently produced in almost all prokaryotic and eukaryotic hosts, for example, E. coli (see U.S. Pat. No. 6,765,087), molds (for example Aspergillus or Trichoderma) and yeast (for example Saccharomyces, Kluyvermyces, Hansenula or Pichia (see U.S. Pat. No. 6,838,254). The production process is scalable and multi-kilogram quantities of nanobodies have been produced. Nanobodies may be formulated as a ready-to-use solution having a long shelf life. The Nanoclone method (see WO 06/079372) is a proprietary method for generating Nanobodies against a desired target, based on automated high-throughput selection of B cells.

In certain embodiments, the antibodies or antigen-binding fragments thereof are humanized. These embodiments refer to a chimeric molecule, generally prepared using recombinant techniques, having an antigen-binding site derived from an immunoglobulin from a non-human species and the remaining immunoglobulin structure of the molecule based upon the structure and/or sequence of a human immunoglobulin. The antigen-binding site may comprise either complete variable domains fused onto constant domains or only the CDRs grafted onto appropriate framework regions in the variable domains. Epitope binding sites may be wild type or modified by one or more amino acid substitutions. This eliminates the constant region as an immunogen in human individuals, but the possibility of an immune response to the foreign variable region remains (LoBuglio et al., PNAS USA 86:4220-4224, 1989; Queen et al., PNAS USA. 86: 10029-10033, 1988; Riechmann et al., Nature. 332:323-327, 1988). Illustrative methods for humanization of antibodies include the methods described in U.S. Patent No. 7,462,697.

Another approach focuses not only on providing human-derived constant regions, but modifying the variable regions as well so as to reshape them as closely as possible to human form. It is known that the variable regions of both heavy and light chains contain three complementarity- determining regions (CDRs) which vary in response to the epitopes in question and determine binding capability, flanked by four framework regions (FRs) which are relatively conserved in a given species and which putatively provide a scaffolding for the CDRs. When nonhuman antibodies are prepared with respect to a particular epitope, the variable regions can be "reshaped" or "humanized" by grafting CDRs derived from nonhuman antibody on the FRs present in the human antibody to be modified. Application of this approach to various antibodies has been reported by Sato et al., Cancer Res. 53:851-856, 1993; Riechmann et al., Nature 332:323-327, 1988; Verhoeyen et al., Science 239: 1534-1536, 1988; Kettleborough et al., Protein Engineering. 4:773-3783, 1991; Maeda et al., Human Antibodies Hybridoma 2: 124-134, 1991; Gorman et al., PNAS USA. 88:4181-4185, 1991; Tempest et al., Bio/Technology 9:266-271, 1991; Co et al., PNAS USA. 88:2869-2873, 1991; Carter et al., PNAS USA. 89:4285-4289, 1992; and Co et al., J Immunol. 148: 1149-1154, 1992. In some embodiments, humanized antibodies preserve all CDR sequences (for example, a humanized mouse antibody which contains all six CDRs from the mouse antibodies). In other embodiments, humanized antibodies have one or more CDRs (one, two, three, four, five, six) which are altered with respect to the original antibody, which are also termed one or more CDRs "derived from" one or more CDRs from the original antibody.

In certain embodiments, the antibodies may be chimeric antibodies. In this regard, a chimeric antibody is comprised of an antigen-binding fragment of an antibody operably linked or otherwise fused to a heterologous Fc portion of a different antibody. In certain embodiments, the heterologous Fc domain is of human origin. In other embodiments, the heterologous Fc domain may be from a different Ig class from the parent antibody, including IgA (including subclasses IgAl and IgA2), IgD, IgE, IgG (including subclasses IgGl, IgG2, IgG3, and IgG4), and IgM. In further embodiments, the heterologous Fc domain may be comprised of CH2 and CH3 domains from one or more of the different Ig classes. As noted above with regard to humanized antibodies, the antigen-binding fragment of a chimeric antibody may comprise only one or more of the CDRs of the antibodies described herein (e.g., 1, 2, 3, 4, 5, or 6 CDRs of the antibodies described herein), or may comprise an entire variable domain (VL, VH or both).

In some embodiments, the immunomodulatory agent is or comprises a "ligand," for example, a natural ligand, of a target molecule. A "ligand" refers generally to a substance or molecule that forms a complex with a target molecule (e.g., biomolecule) to serve a biological purpose, and includes a "protein ligand," which generally produces a signal by binding to a site on a target molecule or target protein. Thus, certain agents are protein ligands that, in nature, bind to an target molecule and produce a signal. Also included are "modified ligands," for example, protein ligands that are fused to a pharmacokinetic modifier, for example, an Fc region derived from an immunoglobulin.

In some embodiments, the immunomodulatory agent or inhibitor is an antisense agent. Thus, in some embodiments, the target proteins, target sequences, and/or target genes described herein (for example, S1PR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, a kinase) are targeted by any variety of antisense agents, including oligonucleotide-based agents or methods. The antisense agents or oligonucleotides typically comprises a base sequence that targets (e.g., is sufficiently complementary to or specifically hybridizes to) a region within a target sequence which optionally includes one or more of the following: a region including or surrounding an AUG start codon of an mRNA (e.g., a region upstream of a start codon, a region downstream of a start codon, a region including a start codon), a 3' or 5' splice site of a pre-processed mRNA, pyrimidine-rich or polypyrimidine tracts upstream of splice acceptor sites, exon-intron boundaries, intron-exon boundaries, a branch sites, exonic splicing enhancer elements, 5' and 3' untranslated regions, and polyadenylation signal sequences.

In certain embodiments, the antisense agent is able to effectively modify expression (e.g., reduce expression, alter splicing) of the target gene upon administration to a subject in need thereof, or upon contact with a cell, for example, a muscle cell. This requirement is typically met when the antisense agent (a) has the ability to be actively taken up by mammalian cells (e.g., muscle cells), and (b) once taken up, forms a duplex with the target RNA with a Tm greater than about 45°C.

Certain "antisense agents" include "antisense oligonucleotides," "antisense oligomers," and "oligonucleotides," which refer to a linear sequence of nucleotides, or nucleotide analogs, which allows the nucleobase to hybridize to a target sequence in an RNA by Watson-Crick base pairing, to form an oligonucleotide :RN A heteroduplex within the target sequence. The terms "antisense oligonucleotide", "antisense oligomer," "oligomer" and "compound" may be used interchangeably to refer to an oligonucleotide. The cyclic subunits may be based on ribose or another pentose sugar or, in certain embodiments, a morpholino group (see description of morpholino oligonucleotides below). Also contemplated are peptide nucleic acids (PNAs), locked nucleic acids (LNAs), tricyclo-DNA oligomers, tricyclo-phosphorothioate oligonucleotides, and 2'-0-Methyl oligonucleotides, among other antisense agents known in the art.

In certain embodiments, the "target sequence" includes a region including or surrounding an AUG start codon of an mRNA (e.g., a region upstream of a start codon, a region downstream of a start codon, a region including a start codon), a 3' or 5' splice site of a pre-processed mRNA, a branch point, or a 3 ' non-coding mRNA region such as a 3 ' -UTR or polyadenylation signal. The target sequence may be within an exon or within an intron. The target sequence for a splice site may include an mRNA sequence having its 5' end 1 to about 25 base pairs downstream of a normal splice acceptor junction in a preprocessed mRNA (pre-mRNA). An exemplary target sequence for a splice region is any region of a preprocessed mRNA that includes a splice site or is contained entirely within an exon coding sequence or spans a splice acceptor or donor site. An antisense agent is more generally said to be "targeted against" a biologically relevant target when it is targeted against, e.g., specifically hybridizes to or is complementary to the nucleic acid of the target in the manner described herein and known in the art. Other examples of target regions or target sequences are described herein.

The term "targeting sequence" is the sequence in the oligonucleotide that is complementary (meaning, in addition, substantially complementary) to the "target sequence" in the RNA. The entire sequence, or only a portion, of the antisense agent may be complementary to the target sequence. For example, in an antisense agent having 20-30 bases, about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, or 29 may be targeting sequences that are complementary to the target region. Typically, the targeting sequence is formed of contiguous bases in the oligonucleotide, but may alternatively be formed of non-contiguous sequences that when placed together, e.g., from opposite ends of the oligonucleotide, constitute sequence that spans the target sequence.

A target sequence may have "near" or "substantial" complementarity to the targeting sequence and still function for the purpose of the present disclosure, that is, still be "complementary." Preferably, the oligonucleotides employed in the present disclosure have at most one mismatch with the target sequence out of 10 nucleotides, and preferably at most one mismatch out of 20.

Alternatively, the antisense oligonucleotide employed have at least 90% sequence homology or identity, at least 95% sequence homology or identity, or at least 98% sequence homology or identity with an exemplary targeting sequence.

Included are non-naturally -occurring oligonucleotides, or "oligonucleotide analogs," including oligonucleotides having (i) a modified backbone structure, e.g., a backbone other than the standard phosphodiester linkage found in naturally -occurring oligo- and polynucleotides, and/or (ii) modified sugar moieties, e.g., morpholino moieties rather than ribose or deoxyribose moieties.

Oligonucleotide analogs support bases capable of hydrogen bonding by Watson-Crick base pairing to standard polynucleotide bases, where the analog backbone presents the bases in a manner to permit such hydrogen bonding in a sequence-specific fashion between the oligonucleotide analog molecule and bases in a standard polynucleotide (e.g., single-stranded RNA or single-stranded DNA). Particular examples of analogs include those having a substantially uncharged, phosphorus containing backbone.

A "nuclease-resistant" oligonucleotide refers to one whose backbone is substantially resistant to nuclease cleavage, in non-hybridized or hybridized form; by common extracellular and intracellular nucleases in the body (for example, by exo nucleases such as 3'-exonucleases, endonucleases, RNase H); that is, the oligonucleotide shows little or no nuclease cleavage under normal nuclease conditions in the body to which the oligonucleotide is exposed. A "nuclease-resistant heteroduplex" refers to a heteroduplex formed by the binding of an antisense oligonucleotide to its complementary target, such that the heteroduplex is substantially resistant to in vivo degradation by intracellular and extracellular nucleases, which are capable of cutting double-stranded RNA/RNA or RNA/DNA complexes. A "heteroduplex" refers to a duplex between an antisense oligonucleotide and the complementary portion of a target RNA.

In certain embodiments, the antisense oligonucleotide is recognized as a substrate for active or facilitated transport across the cell membrane, for example, the muscle cell membrane. The ability of the oligonucleotide to form a stable duplex with the target RNA may also relate to other features of the oligonucleotide backbone, including the length and degree of complementarity of the antisense oligonucleotide with respect to the target, the ratio of G:C to A:T base matches, and the positions of any mismatched bases. The ability of the antisense oligonucleotide to resist cellular nucleases may promote survival and ultimate delivery of the agent to the cell cytoplasm. Thus, certain embodiments include non-naturally -occurring antisense oligonucleotides that are nuclease resistant or substantially nuclease resistant.

In certain embodiments, the antisense oligonucleotide comprises a non-natural chemical backbone selected from a phosphoramidate or phosphorodiamidate morpholino oligonucleotides (PMO), a peptide nucleic acid (PNA), a locked nucleic acid (LNA), a phosphorothioate

oligonucleotide, a tricyclo-DNA oligonucleotide, a tricyclo-phosphorothioate oligonucleotide, a 2'0- Me-modified oligonucleotide (e.g., 2' O-methyl phosphorothioate oligonucleotide), or any combination of the foregoing.

An antisense oligonucleotide "specifically hybridizes" to a target sequence or polynucleotide (e.g., pre-mRNA, mRNA) if the oligonucleotide hybridizes to the target under physiological conditions, with a Tm substantially greater than 40°C or 45°C, preferably at least 50°C, and typically 60°C-80°C or higher. Such hybridization preferably corresponds to stringent hybridization conditions. At a given ionic strength and pH, the Tm is the temperature at which 50% of a target sequence hybridizes to a complementary polynucleotide. Such hybridization may occur with "near" or "substantial" complementarity of the antisense oligonucleotide to the target sequence, as well as with exact complementarity.

As used herein, "sufficient length" refers to an antisense oligonucleotide that is

complementary to at least 8, more typically 8-40, contiguous nucleobases in a target sequence or gene described herein. An antisense oligonucleotide of sufficient length has at least a minimal number of nucleotides to be capable of specifically hybridizing to a region of a target sequence or gene.

Preferably an oligonucleotide of sufficient length is from 8 to 30 nucleotides in length. More preferably, an oligonucleotide of sufficient length is from 9 to 27 nucleotides in length.

Antisense oligonucleotides generally comprise a plurality of nucleotide subunits each bearing a nucleobase which taken together form or comprise a targeting sequence. Accordingly, in some embodiments, the antisense oligonucleotides range in length from about 10 to about 40 subunits, or about 10 to 30 subunits, and typically 15-25 subunits. For example, in some embodiments an antisense oligonucleotide is about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 subunits in length, or ranges from 10 subunits to 40 subunits, 10 subunits to 30 subunits, 14 subunits to 25 subunits, 15 subunits to 30 subunits, 17 subunits to 30 subunits, 17 subunits to 27 subunits, 10 subunits to 27 subunits, 10 subunits to 25 subunits, and 10 subunits to 20 subunits. In certain embodiments, the antisense oligonucleotide is about 10 to about 40 or about 5 to about 30 nucleotides in length. In some embodiments, the antisense oligonucleotide is about 14 to about 25 or about 17 to about 27 nucleotides in length.

In some embodiments, the backbone of the antisense oligonucleotide is substantially uncharged, and is optionally recognized as a substrate for active or facilitated transport across the cell membrane. In some embodiments, all the internucleotide linkages are uncharged. The ability of the oligonucleotide to form a stable duplex with the target RNA may also relate to other features of the backbone, including the length and degree of complementarity of the antisense oligonucleotide with respect to the target, the ratio of G:C to A:T base matches, and the positions of any mismatched bases. The ability of the antisense oligonucleotide to resist cellular nucleases may promote survival and ultimate delivery of the agent to the cell cytoplasm.

In certain embodiments, the antisense oligonucleotide has at least one intemucleoside linkage that is positively charged or cationic at physiological pH. In some embodiments, the antisense oligonucleotide has at least one intemucleoside linkage that exhibits a pKa between about 5.5 and about 12. Optionally, the antisense oligonucleotide has at least one intemucleoside linkage with both a basic nitrogen and an alkyl, aryl, or aralkyl group. In particular embodiments, the cationic intemucleoside linkage or linkages comprise a 4-aminopiperdin-l-yl (APN) group, or a derivative thereof. While not being bound by any one theory, it is believed that the presence of a cationic linkage or linkages (e.g., APN group or APN derivative) in the oligonucleotide facilitates binding to the negatively charged phosphates in the target nucleotide. Thus, the formation of a heteroduplex between mutant RNA and the cationic linkage-containing oligonucleotide may be held together by both an ionic attractive force and Watson-Crick base pairing.

In some embodiments, the number of cationic linkages is at least 2 and no more than about half the total intemucleoside linkages, e.g., about or no more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 cationic linkages. In some embodiments, however, up to all of the intemucleoside linkages are cationic linkages, e.g., about or at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 of the total intemucleoside linkages are cationic linkages. In specific embodiments, an oligonucleotide of about 19-20 subunits may have 2-10, e.g., 4-8, cationic linkages, and the remainder uncharged linkages. In other specific embodiments, an oligonucleotide of 14-15 subunits may have 2- 7, e.g., 2, 3, 4, 5, 6, or 7 cationic linkages and the remainder uncharged linkages. The total number of cationic linkages in the oligonucleotide can thus vary from about 1 to 10 to 15 to 20 to 30 or more (including all integers in between), and can be interspersed throughout the oligonucleotide.

In some embodiments, an antisense oligonucleotide may have about or up to about 1 cationic linkage per every 2-5 or 2, 3, 4, or 5 uncharged linkages, such as about 4-5 or 4 or 5 per every 10 uncharged linkages.

Certain embodiments include antisense oligonucleotides that contain about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% cationic linkages. In certain embodiments, optimal improvement in antisense activity may be seen if about 25% of the backbone linkages are cationic. In certain embodiments, enhancement may be seen with a small number e.g., 10-20% cationic linkages, or where the number of cationic linkages are in the range 50-80%, such as about 60%.

In some embodiments, the cationic linkages are interspersed along the backbone. Such oligonucleotides optionally contain at least two consecutive uncharged linkages; that is, the oligonucleotide optionally does not have a strictly alternating pattern along its entire length. In specific instances, each one or two cationic linkage(s) is/are separated along the backbone by at least 1, 2, 3, 4, or 5 uncharged linkages.

Also included are oligonucleotides having blocks of cationic linkages and blocks of uncharged linkages. For example, a central block of uncharged linkages may be flanked by blocks of cationic linkages, or vice versa. In some embodiments, the oligonucleotide has approximately equal- length 5', 3' and center regions, and the percentage of cationic linkages in the center region is greater than about 50%, 60%, 70%, or 80% of the total number of cationic linkages.

In certain antisense oligonucleotides, the bulk of the cationic linkages (e.g., 70, 75%, 80%, 90% of the cationic linkages) are distributed close to the "center-region" backbone linkages, e.g., the 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 centermost linkages. For example, a 16, 17, 18, 19, 20, 21, 22, 23, or 24-mer oligonucleotide with may have at least 50%, 60%, 70%, or 80% of the total cationic linkages localized to the 8, 9, 10, 11, or 12 centermost linkages.

As noted above, the antisense oligonucleotides can employ a variety of antisense chemistries. Examples of oligonucleotide chemistries include, without limitation, peptide nucleic acid (PNA), locked nucleic acid (LNA), phosphorothioate, 2 'O-Me-modified oligonucleotides, morpholino, PMO, PPMO, PMOplus, and PMO-X chemistries, including combinations of any of the foregoing. In general, PNA and LNA chemistries can utilize shorter targeting sequences because of their relatively high target binding strength relative to PMO and 2'O-Me oligonucleotides. Phosphorothioate and 2 'O-Me-modified chemistries are often combined to generate a 2'0-Me-phosphorothioate backbone. See, e.g., PCT Publication Nos. WO/2013/112053 and WO/2009/008725, incorporated herein by reference in their entireties.

Peptide nucleic acids (PNAs) are analogs of DNA in which the backbone is structurally homomorphous with a deoxyribose backbone, consisting of N-(2-aminoethyl) glycine units to which pyrimidine or purine bases are attached. PNAs containing natural pyrimidine and purine bases hybridize to complementary oligonucleotides obeying Watson-Crick base-pairing rules, and mimic DNA in terms of base pair recognition (Egholm, Buchardt et al. 1993). The backbone of PNAs is formed by peptide bonds rather than phosphodiester bonds, making them well-suited for antisense applications (see structure below). The backbone is uncharged, resulting in PNA/DNA or PNA RNA duplexes that exhibit greater than normal thermal stability. PNAs are not recognized by nucleases or proteases.

Despite a radical structural change to the natural structure, PNAs are capable of sequence- specific binding in a helix form to DNA or RNA. Characteristics of PNAs include a high binding affinity to complementary DNA or RNA, a destabilizing effect caused by single-base mismatch, resistance to nucleases and proteases, hybridization with DNA or RNA independent of salt concentration and triplex formation with homopurine DNA. PANAGENE™ has developed Bts PNA monomers (Bts; benzothiazole-2-sulfonyl group) and an oligomerization process. The PNA oligomerization using Bts PNA monomers is composed of repetitive cycles of deprotection, coupling and capping. PNAs can be produced synthetically using any technique known in the art. See, e.g., U.S. Pat. Nos. 6,969,766, 7,211,668, 7,022,851, 7,125,994, 7,145,006 and 7,179,896. See also U.S. Pat. Nos. 5,539,082; 5,714,331; and 5,719,262 for the preparation of PNAs. Further teaching of PNA compounds can be found in Nielsen et al., Science, 254: 1497-1500, 1991. Each of the foregoing is incorporated by reference in its entirety.

Antisense oligonucleotide compounds may also contain "locked nucleic acid" subunits (LNAs). "LNAs" are a member of a class of modifications called bridged nucleic acid (BNA). BNA is characterized by a covalent linkage that locks the conformation of the ribose ring in a C30-endo (northern) sugar pucker. For LNA, the bridge is composed of a methylene between the 2'-0 and the 4'-C positions. LNA enhances backbone preorganization and base stacking to increase hybridization and thermal stability. The structures of LNAs can be found, for example, in Wengel, et al., Chemical Communications (1998) 455; Tetrahedron (1998) 54:3607, and Accounts of Chem. Research (1999) 32:301); Obika, et al., Tetrahedron Letters (1997) 38:8735; (1998) 39:5401, and Bioorganic

Medicinal Chemistry (2008) 16:9230.

Compounds of the disclosure may incorporate one or more LNAs; in some cases, the compounds may be entirely composed of LNAs. Methods for the synthesis of individual LNA nucleoside subunits and their incorporation into oligonucleotides are described, for example, in U.S. Pat. Nos. 7,572,582, 7,569,575, 7,084,125, 7,060,809, 7,053,207, 7,034,133, 6,794,499, and

6,670,461, each of which is incorporated by reference in its entirety. Typical intersubunit linkers include phosphodiester and phosphorothioate moieties; alternatively, non-phosphorous containing linkers may be employed. One embodiment is an LNA containing compound where each LNA subunit is separated by a DNA subunit. Certain compounds are composed of alternating LNA and DNA subunits where the intersubunit linker is phosphorothioate. "Phosphorothioates" (or S-oligos) are a variant of normal DNA in which one of the nonbridging oxygens is replaced by a sulfur. The sulfurization of the internucleotide bond reduces the action of endo-and exonucleases including 5' to 3' and 3' to 5' DNA POL 1 exonuclease, nucleases SI and PI, RNases, serum nucleases and snake venom phosphodiesterase. Phosphorothioates are made by two principal routes: by the action of a solution of elemental sulfur in carbon disulfide on a hydrogen phosphonate, or by the method of sulfurizing phosphite triesters with either

tetraethylthiuram disulfide (TETD) or 3H-l, 2-bensodithiol-3-one 1, 1-dioxide (BDTD) (see, e.g., Iyer et al., J. Org. Chem. 55, 4693-4699, 1990). The latter methods avoid the problem of elemental sulfur's insolubility in most organic solvents and the toxicity of carbon disulfide. The TETD and BDTD methods also yield higher purity phosphorothioates.

Tricyclo-DNAs (tc-DNA) are a class of constrained DNA analogs in which each nucleotide is modified by the introduction of a cyclopropane ring to restrict conformational flexibility of the backbone and to optimize the backbone geometry of the torsion angle γ. Homobasic adenine- and thymine-containing tc-DNAs form extraordinarily stable A-T base pairs with complementary RNAs. Tricyclo-DNAs and their synthesis are described in International Patent Application Publication No. WO 2010/115993. Compounds of the disclosure may incorporate one or more tricycle-DNA nucleotides; in some cases, the compounds may be entirely composed of tricycle-DNA nucleotides.

Tricyclo-phosphorothioate nucleotides are tricyclo-DNA nucleotides with phosphorothioate intersubunit linkages. Tricyclo-phosphorothioate nucleotides and their synthesis are described in International Patent Application Publication No. WO 2013/053928. Compounds of the disclosure may incorporate one or more tricycle-DNA nucleotides; in some cases, the compounds may be entirely composed of tricycle-DNA nucleotides.

"2'O-Me oligonucleotides" molecules carry a methyl group at the 2'-OH residue of the ribose molecule. 2'-0-Me-RNAs show the same (or similar) behavior as DNA, but are protected against nuclease degradation. 2'-0-Me-RNAs can also be combined with phosphorothioate oligonucleotides (PTOs) for further stabilization. 2'O-Me oligonucleotides (phosphodiester or phosphothioate) can be synthesized according to routine techniques in the art (see, e.g., Yoo et al., Nucleic Acids Res.

32:2008-16, 2004). In some instances, 2' O-Me oligonucleotides comprise a phosphorothioate linkage (2' O-Me phosphorothioate oligonucleotides).

A "morpholino oligonucleotide" or "PMO" refers to an oligonucleotide having a backbone which supports a nucleobase capable of hydrogen bonding to typical polynucleotides, wherein the polymer lacks a pentose sugar backbone moiety, but instead contains a morpholino ring. Thus, in a PMO a morpholino ring structure supports a base pairing moiety, to form a sequence of base pairing moieties which is typically designed to hybridize to a selected antisense target in a cell or in a subject being treated. An exemplary "morpholino" oligonucleotide comprises morpholino subunit structures linked together by phosphoramidate or phosphorodiamidate linkages, joining the morpholino nitrogen of one subunit to the 4' exocyclic carbon of an adjacent subunit, each subunit comprising a purine or pyrimidine nucleobase effective to bind, by base-specific hydrogen bonding, to a base in a polynucleotide. Morpholino oligonucleotides (including antisense oligonucleotides) are detailed, for example, in U.S. Pat. Nos. 5,698,685; 5,217,866; 5,142,047; 5,034,506; 5,166,315; 5,185,444;

5,521,063; 5,506,337 and pending US patent applications 12/271,036; 12/271,040; and PCT publication numbers WO/2009/064471 and WO/2012/043730, all of which are incorporated herein by reference in their entirety.

Within the oligonucleotide structure, the phosphate groups are commonly referred to as forming the "internucleoside linkages" of the oligonucleotide. The naturally occurring internucleoside linkage of RNA and DNA is a 3' to 5' phosphodiester linkage. A "phosphoramidate" group comprises phosphorus having three attached oxygen atoms and one attached nitrogen atom, while a

"phosphorodiamidate" group comprises phosphorus having two attached oxygen atoms and two attached nitrogen atoms. In the uncharged or the cationic intersubunit linkages of the PMO and/or PMO-X oligonucleotides described herein, one nitrogen is always pendant to the backbone chain. The second nitrogen, in a phosphorodiamidate linkage, is typically the ring nitrogen in a morpholino ring structure.

"PMO-X" refers to phosphorodiamidate morpholino oligonucleotides (PMOs) having a phosphorus atom with (i) a covalent bond to the nitrogen atom of a morpholino ring and (ii) a second covalent bond to the ring nitrogen of a 4-aminopiperdin-l-yl (i.e., APN) or a derivative of 4- aminopiperdin-l-yl. Exemplary PMO-X oligonucleotides are disclosed in PCT application No.

PCT/US2011/38459 and PCT Publication No. WO/2013/074834, each of which is herein incorporated by reference in its entirety. "PMO-apn" or "APN" refers to a PMO-X oligonucleotide which comprises at least one internucleoside linkage where a phosphorus atom is linked to a morpholino group and to the ring nitrogen of a 4-aminopiperdin-l-yl (i.e., APN). In specific embodiments, an antisense oligonucleotide comprising a targeting sequence described herein comprises at least one APN-containing linkage or APN derivative-containing linkage. Specific embodiments include PMOs that have about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% APN/ APN derivative-containing linkages, where the remaining linkages (if less than 100%) are uncharged linkages, e.g., about or at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 of the total internucleoside linkages are APN/ APN derivative-containing linkages.

Additional antisense oligonucleotides/chemistries that can be used in accordance with the methods and compositions provided herein include those described in the following patents and patent publications, the contents of which are incorporated herein by reference: PCT Publication Nos.

WO/2007/002390; WO/2010/120820; and WO/2010/148249; U.S. Patent No. 7,838,657; and U.S. Application No. 2011/0269820.

In some embodiments, the the immunomodulatory agent or inhibitor is an RNA interference (RNAi) agent. Thus, in some embodiments, the target proteins, target sequences, and/or target genes described herein (for example, S1PR, calcineurin, mTOR, IDO, IMPDH, a cytokine and/or cytokine receptor, a B cell receptor, a kinase) are targeted by any variety of RNA interference-based agents or methods. RNA interference (RNAi) is an evolutionarily conserved gene-silencing mechanism, originally discovered in studies of the nematode Caenorhabditis elegans (Lee et al, Cell 75:843,1993; Reinhart et al., Nature 403:901, 2000). It can be triggered by introducing dsRNA into cells expressing the appropriate molecular machinery, which then degrades the corresponding endogenous mRNA. The mechanism involves conversion of dsRNA into short RNAs that direct ribonucleases to homologous mRNA targets (see Ruvkun, Science 2294:797, 2001).

In certain embodiments, an RNA agent is more generally said to be "targeted against" a biologically relevant target (gene) when it comprises a sense strand that corresponds to the "target sequence" of the target gene, in a manner described herein and known in the art.

RNAi agents include RNAi nucleic acid molecules and RNAi nucleic acid analogue molecules, such as short interfering nucleic acids and short interfering nucleic acid analogues (siNA), including short interfering RNA and short interfering RNA nucleic acid analogues (siRNA), and including, for example, double-stranded RNA and double-stranded RNA analogues (dsRNA), micro- RNA and micro-RNA analogues (miRNA), and short hairpin RNA and short hairpin RNA analogues (shRNA).

Certain embodiments employ double-stranded ribonucleic acid (dsRNA) molecules as RNAi agents. dsRNAs generally comprise two single strands. One strand of the dsRNA comprises a nucleotide sequence that is substantially identical to a portion of the target gene or target sequence (the "sense" strand), and the other strand (the "complementary" or "antisense" strand) comprises a sequence that is complementary or substantially complementary to a portion of the target region. Substantially identical sequences include those that are at least about 80, 85, 90, 95, 97, 98, 99% identical to the target sequence. The strands are sufficiently complementary to hybridize to form a duplex structure. In certain embodiments, the complementary RNA strand may be less than 30 nucleotides, less than 25 nucleotides in length, or even 19 to 24 nucleotides in length. In certain aspects, the complementary nucleotide sequence may be 20-23 nucleotides in length, or 22 nucleotides in length. In certain embodiments, the sense strand of the RNAi agent is substantially identical to a portion of a target sequence described herein. In some embodiments, the antisense strand is complementary or substantially complementary to a portion of target sequence described herein. In certain embodiments, said portion comprises, consists, or consists essentially of about, at least about, or no more than about 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 contiguous nucleotides of a target sequence described herein.

Suitable siRNA sequences can be identified using any means known in the art. In some instances, using the exemplary target sequences described herein, the methods described in Elbashir et al., Nature, 411:494-498 (2001) and Elbashir et al., EMBO I, 20:6877-6888 (2001) are combined with rational design rules set forth in Reynolds et al., Nature Biotech., 22:326-330 (2004). Generally, the nucleotide sequence 3 ' of the AUG start codon of a transcript from the target gene of interest is scanned for dinucleotide sequences (e.g., AA, NA, CC, GG, or UU, wherein N=C, G, or U) (see, e.g., Elbashir et al., EMBO J., 20:6877-6888 (2001)). The nucleotides immediately 3' to the dinucleotide sequences are identified as potential siRNA sequences (i.e., a target sequence or a sense strand sequence). In some instances, the 19, 21, 23, 25, 27, 29, 31, 33, 35, or more nucleotides immediately 3 ' to the dinucleotide sequences are identified as potential siRNA sequences. In some embodiments, the dinucleotide sequence is an AA or NA sequence and the 19 nucleotides immediately 3 ' to the AA or NA dinucleotide are identified as a potential siRNA sequences. siRNA sequences are usually spaced at different positions along the length of the target gene. To further enhance silencing efficiency of the siRNA sequences, potential siRNA sequences may be analyzed to identify sites that do not contain regions of homology to other coding sequences, e.g., in the target cell or organism. For example, a suitable siRNA sequence of about 21 base pairs typically will not have more than 16-17 contiguous base pairs of homology to coding sequences in the target cell or organism. If the siRNA sequences are to be expressed from an RNA Pol III promoter, siRNA sequences lacking more than 4 contiguous A's or T's are selected.

Once a potential siRNA sequence has been identified, the sequence can be analyzed using a variety of criteria known in the art. For example, to enhance their silencing efficiency, the siRNA sequences may be analyzed by a rational design algorithm to identify sequences that have one or more of the following features: (1) G/C content of about 25% to about 60% G/C; (2) at least 3 A/Us at positions 15-19 of the sense strand; (3) no internal repeats; (4) an A at position 19 of the sense strand; (5) an A at position 3 of the sense strand; (6) a U at position 10 of the sense strand; (7) no G/C at position 19 of the sense strand; and (8) no G at position 13 of the sense strand. siRNA design tools that incorporate algorithms that assign suitable values of each of these features and are useful for selection of siRNA are known. One of skill in the art will appreciate that sequences with one or more of the foregoing characteristics may be selected for further analysis and testing as potential siRNA sequences.

Additionally, potential siRNA target sequences with one or more of the following criteria can often be eliminated as siRNA: (1) sequences comprising a stretch of 4 or more of the same base in a row; (2) sequences comprising homopolymers of Gs (i.e., to reduce possible non-specific effects due to structural characteristics of these polymers; (3) sequences comprising triple base motifs (e.g., GGG, CCC, AAA, or TTT); (4) sequences comprising stretches of 7 or more G/Cs in a row; and (5) sequences comprising direct repeats of 4 or more bases within the candidates resulting in internal fold-back structures. However, one of skill in the art will appreciate that sequences with one or more of the foregoing characteristics may still be selected for further analysis and testing as potential siRNA sequences.

In some embodiments, potential siRNA target sequences may be further analyzed based on siRNA duplex asymmetry as described in, e.g., Khvorova et al., Cell, 115:209-216 (2003); and Schwarz et al., Cell, 115: 199-208 (2003). In certain embodiments, potential siRNA target sequences may be further analyzed based on secondary structure at the mRNA target site as described in, e.g., Luo et al., Biophys. Res. Commun., 318:303-310 (2004). For example, mRNA secondary structure can be modeled using the Mfold algorithm to select siRNA sequences which favor accessibility at the mRNA target site where less secondary structure in the form of base-pairing and stem-loops is present.

A potential siRNA sequence can also be analyzed for the presence of any immunostimulatory properties, e.g., using an in vitro cytokine assay or an in vivo animal model. Motifs in the sense and/or antisense strand of the siRNA sequence such as GU-rich motifs (e.g., 5'-GU-3',5'-UGU-3',5'- GUGU-3',5'-UGUGU-3', etc.) can also provide an indication of whether the sequence may be immunostimulatory. Once an siRNA molecule is found to be immunostimulatory, it can then be modified to decrease its immunostimulatory properties as described herein. As a non-limiting example, an siRNA sequence can be contacted with a mammalian responder cell under conditions such that the cell produces a detectable immune response to determine whether the siRNA is an immunostimulatory or a non-immunostimulatory siRNA. The mammalian responder cell may be from a naive mammal (i.e., a mammal that has not previously been in contact with the siRNA sequence). The mammalian responder cell may be, e.g., a peripheral blood mononuclear cell (PBMC), a macrophage, and the like. The detectable immune response may comprise production of a cytokine or growth factor such as, e.g., TNF-alpha, IFN-alpha, IFN-beta, IFN-gamma, IL-6, IL-12, or a combination thereof. An siRNA molecule identified as being immunostimulatory can then be modified to decrease its immunostimulatory properties by replacing at least one of the nucleotides on the sense and/or antisense strand with modified nucleotides. For example, less than about 30% (e.g., less than about 30%, 25%, 20%, 15%, 10%, or 5%) of the nucleotides in the double-stranded region of the siRNA duplex can be replaced with modified nucleotides such as 2'OMe nucleotides. The modified siRNA can then be contacted with a mammalian responder cell as described above to confirm that its immunostimulatory properties have been reduced or abrogated.

An RNAi agent typically includes a double stranded portion (notwithstanding the optional and potentially preferred presence of any single-stranded overhands) comprising at least 16 bases, optionally at least 17 bases, more optionally at least 18 bases and still more optionally at least 19 bases, and usually between 18 and 35 bases, optionally between 19 and 30 bases, more optionally between 20 and 25 bases and even more optionally between 21 and 23 bases which are identical or almost identical to (e.g., showing 90% or more, e.g., at least 95%, sequence identity to, or showing maximum 2 and optionally only 1 mismatch with) an mRNA whose silencing is desired and which is thus targeted by said RNAi agent.

In certain embodiments, at least one of the RNA strands comprises a nucleotide overhang of 1 to 4 nucleotides in length. In some embodiments, the dsRNA comprises at least one chemically modified nucleotide. In certain aspects, a dsRNA comprising a single-stranded overhang of 1 to 4 nucleotides may comprise a molecule wherein the unpaired nucleotide of the single-stranded overhang that is directly adjacent to the terminal nucleotide pair contains a purine base. In some embodiments, the last complementary nucleotide pairs on both ends of a dsRNA are a G-C pair, or, at least two of the last four terminal nucleotide pairs are G-C pairs.

Certain embodiments RNAi agents include microRNAs. Micro-RNAs represent a large group of small RNAs produced naturally in organisms, some of which regulate the expression of target genes. Micro-RNAs are formed from an approximately 70 nucleotide single -stranded hairpin precursor transcript by Dicer, (see V. Ambros et al., Current Biology 13 :807, 2003). Micro-RNAs are not translated into proteins, but instead bind to specific messenger RNAs, thereby blocking translation. It is thought that micro-RNAs base-pair imprecisely with their targets to inhibit translation. Certain micro-RNAs may be transcribed as hairpin RNA precursors, which are then processed to their mature forms by Dicer enzyme.

In certain embodiments, the RNAi agent or oligonucleotide is single stranded. In some embodiments, the RNAi agent or oligonucleotide is double stranded. Certain embodiments include short-interfering RNAs (siRNA). In certain embodiments, the first strand of the double-stranded oligonucleotide contains two more nucleoside residues than the second strand. In other embodiments, the first strand and the second strand have the same number of nucleosides; however, the first and second strands are offset such that the two terminal nucleosides on the first and second strands are not paired with a residue on the complimentary strand. In certain instances, the two nucleosides that are not paired are thymidine resides.

In some instances where the modulating agent comprises siRNA, the agent includes a region of sufficient homology to the target region, and is of sufficient length in terms of nucleotides, such that the siRNA agent, or a fragment thereof, mediates down-regulation of the target gene or RNA. It will be understood that the term "ribonucleotide" or "nucleotide" can, in the case of a modified RNA or nucleotide surrogate, also refer to a modified nucleotide, or surrogate replacement moiety at one or more positions. Thus, an siRNA agent is or includes a region which is at least partially

complementary to the target sequence. It is not necessary that there be perfect complementarity between the siRNA agent and the target sequence, but the correspondence must be sufficient to enable the siRNA agent, or a cleavage product thereof, to direct sequence specific silencing, such as by RNAi cleavage of the target RNA. Complementarity, or degree of homology with the target strand, is most critical in the antisense strand. While perfect complementarity, particularly in the antisense strand, is often desired, some embodiments include one or more but preferably 10, 8, 6, 5, 4, 3, 2, or fewer mismatches with respect to the target sequence. The mismatches are most tolerated in the terminal regions, and if present are preferably in a terminal region or regions, e.g., within 6, 5, 4, or 3 nucleotides of the 5' and/or 3' terminus. The sense strand need only be sufficiently complementary with the antisense strand to maintain the overall double-strand character of the molecule. In some embodiments, an RNAi agent or oligonucleotide, e.g., an siRNA oligonucleotide, is modified or includes nucleoside surrogates. Single stranded regions of an siRNA agent may be modified or include nucleoside surrogates, e.g., the unpaired region or regions of a hairpin structure, e.g., a region which links two complementary regions, can have modifications or nucleoside surrogates. Modifications to stabilize one or more 3'- or 5'-terminus of an siRNA agent, e.g., against exonucleases, or to favor the antisense siRNA agent to enter into RISC, are also included. Exemplary modifications include C3 (or C6, C7, C12) amino linkers, thiol linkers, carboxyl linkers, non- nucleotidic spacers (C3, C6, C9, C12, abasic, triethylene glycol, hexaethylene glycol), and special biotin or fluorescein reagents that come as phosphoramidites and that have another DMT-protected hydroxyl group, allowing multiple couplings during RNA synthesis.

Certain siRNA agents include, for example, molecules that are long enough to trigger an interferon response (which can be cleaved by Dicer (Bernstein et al. 2001. Nature, 409:363-366) and enter a RISC (RNAi-induced silencing complex)). Also included are molecules which are sufficiently short that they do not trigger the interferon response (which molecules can also be cleaved by Dicer and/or enter a RISC), e.g., molecules which are of a size which allows entry into a RISC, e.g., molecules which resemble Dicer-cleavage products. Molecules that are short enough that they do not trigger an interferon response are termed siRNA agents or shorter RNAi agents herein. "siRNA agent or shorter RNAi agent" as used refers to an siRNA agent that is sufficiently short that it does not induce a deleterious interferon response in a human cell, e.g., it has a duplexed region of less than 60 but preferably less than 50, 40, or 30 nucleotide pairs. An siRNA modulating agent, or a cleavage product thereof, can down regulate a target gene, e.g., by inducing RNAi with respect to a target RNA.

In some instances, each strand of an siRNA agent is about or less than about 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, or 15 nucleotides in length. For example, each strand can be between about 21 and 25 nucleotides in length. Specific siRNA agents have a duplex region of about 17, 18, 19, 29, 21, 22, 23, 24, or 25 nucleotide pairs, and one or more overhangs, for example, one or two 3' overhangs, of 1-3 nucleotides.

In addition to homology to target RNA and the ability to down regulate a target gene, an siRNA agent may have one or more of the following properties: it may, despite modifications, even to a very large number, or all of the nucleosides, have an antisense strand that can present bases (or modified bases) in the proper three dimensional framework so as to be able to form correct base pairing and form a duplex structure with a homologous target RNA which is sufficient to allow down regulation of the target, e.g., by cleavage of the target RNA; it may, despite modifications, even to a very large number, or all of the nucleosides, still have "RNA-like" properties, i.e., it may possess the overall structural, chemical and physical properties of an RNA molecule, even though not exclusively, or even partly, of ribonucleotide-based content. For example, an siRNA agent can contain, e.g., a sense and/or an antisense strand in which all of the nucleotide sugars contain e.g., 2' fluoro in place of 2' hydroxyl. This deoxyribonucleotide-containing agent can still be expected to exhibit RNA-like properties. While not wishing to be bound by theory, the electronegative fluorine prefers an axial orientation when attached to the C2' position of ribose. This spatial preference of fluorine can, in turn, force the sugars to adopt a C3'-endo pucker. This is the same puckering mode as observed in RNA molecules and gives rise to the RNA-characteristic A-family-type helix. Further, since fluorine is a good hydrogen bond acceptor, it can participate in the same hydrogen bonding interactions with water molecules that are known to stabilize RNA structures. Generally, it is preferred that a modified moiety at the 2' sugar position will be able to enter into H-bonding which is more characteristic of the OH moiety of a ribonucleotide than the H moiety of a deoxy ribonucleotide.

A "single strand RNAi agent" as used herein, is an RNAi agent which is made up of a single molecule. It may include a duplexed region, formed by intra-strand pairing, e.g., it may be, or include, a hairpin or pan-handle structure. Single strand RNAi modulating agents are preferably antisense with regard to the target molecule. A single strand RNAi agent should be sufficiently long that it can enter the RISC and participate in RISC mediated cleavage of a target mRNA. A single strand RNAi agent is at least 14, and more preferably at least 15, 20, 25, 29, 35, 40, or 50 nucleotides in length. It is preferably less than 200, 100, or 60 nucleotides in length.

Hairpin RNAi agents may have a duplex region equal to or at least 17, 18, 19, 29, 21, 22, 23, 24, or 25 nucleotide pairs. The duplex region may preferably be equal to or less than 200, 100, or 50, in length. Certain ranges for the duplex region are 15-30, 17 to 23, 19 to 23, and 19 to 21 nucleotides pairs in length. The hairpin may have a single strand overhang or terminal unpaired region, preferably the 3', and preferably of the antisense side of the hairpin. In certain embodiments, overhangs are 2-3 nucleotides in length.

Certain modulating agents utilized according to the methods provided herein may comprise RNAi oligonucleotides such as chimeric oligonucleotides, or "chimeras," which contain two or more chemically distinct regions, each made up of at least one monomer unit, i.e., a nucleotide in the case of an oligonucleotide compound. These oligonucleotides typically contain at least one region wherein the oligonucleotide is modified so as to confer upon the oligonucleotide increased resistance to nuclease degradation, increased cellular uptake, and/or increased binding affinity for the target nucleic acid. Consequently, comparable results can often be obtained with shorter oligonucleotides when chimeric oligonucleotides are used, compared to phosphorothioate oligodeoxynucleotides. Chimeric oligonucleotides may be formed as composite structures of two or more oligonucleotides, modified oligonucleotides, oligonucleotides and/or oligonucleotide mimetics as described above. Such oligonucleotides have also been referred to in the art as hybrids or gapmers. Representative U.S. patents that teach the preparation of such hybrid structures include, but are not limited to, U.S. Pat. Nos. 5,013,830; 5,149,797; 5,220,007; 5,256,775; 5,366,878; 5,403,711; 5,491,133; 5,565,350; 5,623,065; 5,652,355; 5,652,356; 5,700,922; and 5,955,589, each of which is herein incorporated by reference. In certain embodiments, the chimeric oligonucleotide is RNA-DNA, DNA-RNA, RNA- DNA-RNA, DNA-RNA-DNA, or RNA-DNA-RNA-DNA, wherein the oligonucleotide is between 5 and 60 nucleotides in length.

In some aspects, RNAi agents include an oligonucleotide comprising at least one ligand tethered to an altered or non-natural nucleobase. A large number of compounds can function as the altered base. The structure of the altered base is important to the extent that the altered base should not substantially prevent binding of the oligonucleotide to its target, e.g., mRNA. In certain embodiments, the altered base is difluorotolyl, nitropyrrolyl, nitroimidazolyl, nitroindolyl, napthalenyl, anthrancenyl, pyridinyl, quinolinyl, pyrenyl, or the divalent radical of any one of the non-natural nucleobases described herein. In certain embodiments, the non-natural nucleobase is difluorotolyl, nitropyrrolyl, or nitroimidazolyl. In certain embodiments, the non-natural nucleobase is difluorotolyl. A wide variety of ligands are known in the art. For example, the ligand can be a steroid, bile acid, lipid, folic acid, pyridoxal, B12, riboflavin, biotin, aromatic compound, poly cyclic compound, crown ether, intercalator, cleaver molecule, protein-binding agent, or carbohydrate. In certain embodiments, the ligand is a steroid or aromatic compound. In certain instances, the ligand is cholesteryl.

In some embodiments, the RNAi agent is an oligonucleotide tethered to a ligand for the purposes of improving cellular targeting and uptake. For example, an RNAi agent may be tethered to an antibody, or antigen binding fragment thereof. As an additional example, an RNAi agent may be tethered to a specific ligand binding molecule, such as a polypeptide or polypeptide fragment that specifically binds a particular cell-surface receptor.

In certain embodiments, the RNAi agent comprises a non-natural nucleobase. In some embodiments, the non-natural nucleobase is difluorotolyl, nitroimidazolyl, nitroindolyl, or nitropyrrolyl. In certain embodiments, the modulating agents provided herein relate to a double- stranded oligonucleotide sequence, wherein only one of the two strands contains a non-natural nucleobase. In certain embodiments, the modulating agents as used herein relate to a double-stranded oligonucleotide sequence, wherein both of the strands independently comprise at least one non-natural nucleobase.

In certain instances, the ribose sugar moiety that naturally occurs in nucleosides is replaced with a hexose sugar. In certain aspects, the hexose sugar is an allose, altrose, glucose, mannose, gulose, idose, galactose, talose, or a derivative thereof. In a preferred embodiment, the hexose is a D- hexose. In certain instances, the ribose sugar moiety that naturally occurs in nucleosides is replaced with a polycyclic heteroalkyl ring or cyclohexenyl group. In certain instances, the polycyclic heteroalkyl group is a bicyclic ring containing one oxygen atom in the ring. In certain instances, the polycyclic heteroalkyl group is a bicyclo[2.2.1]heptane, a bicyclo[3.2.1]octane, or a

bicyclo[3.3.1]nonane. In certain embodiments, the backbone of the oligonucleotide has been modified to improve the therapeutic or diagnostic properties of the oligonucleotide compound. In certain embodiments, at least one of the bases or at least one of the sugars of the oligonucleotide has been modified to improve the therapeutic or diagnostic properties of the oligonucleotide compound. In instances when the oligonucleotide is double stranded, the two strands are complementary, partially complementary, or chimeric oligonucleotides.

Examples of modified RNAi agents include oligonucleotides containing modified backbones or non-natural internucleoside linkages. As defined herein, oligonucleotides having modified backbones or internucleoside linkages include those that retain a phosphorus atom in the backbone and those that do not have a phosphorus atom in the backbone. Modified oligonucleotides that do not have a phosphorus atom in their intersugar backbone can also be considered to be oligonucleotides. Specific oligonucleotide chemical modifications are described below. It is not necessary for all positions in a given compound to be uniformly modified, and in fact more than one of the following modifications may be incorporated in a single oligonucleotide compound or even in a single nucleotide thereof.

Examples of modified internucleoside linkages or backbones include, for example, phosphorothioates, chiral phosphorothioates, phosphorodithioates, phosphotriesters,

aminoalkylphosphotriesters, methyl and other alkyl phosphonates including 3 '-alkylene phosphonates and chiral phosphonates, phosphinates, phosphoramidates including 3 '-amino phosphoramidate and aminoalkylphosphoramidates, thionophosphoramidates, thionoalkylphosphonates,

thionoalklyphosphotriesters, and boranophosphates having normal 3 '-5' linkages, 2 '-5' linked analogs of these, and those having inverted polarity wherein the adjacent pairs of nucleoside units are linked 3 '-5' to 5'-3 ' or 2'-5' to 5'-2'. Various salts, mixed salts and free-acid forms are also included.

Representative U.S. patents that teach the preparation of the above phosphorus atom- containing linkages include, but are not limited to, U.S. Pat. Nos. 3,687,808; 4,469,863; 4,476,301; 5,023,243; 5, 177,196; 5, 188,897; 5,264,423; 5,276,019; 5,278,302; 5,286,717; 5,321,131; 5,399,676; 5,405,939; 5,453,496; 5,455,233; 5,466,677; 5,476,925; 5,519,126; 5,536,821; 5,541,306; 5,550, 111; 5,563,253; 5,571,799; 5,587,361; 5,625,050; and 5,697,248, each of which is herein incorporated by reference.

Examples of modified internucleoside linkages or backbones that do not include a phosphorus atom therein (i.e., oligonucleotides) have backbones that are formed by short chain alkyl or cycloalkyl intersugar linkages, mixed heteroatom and alkyl or cycloalkyl intersugar linkages, or one or more short chain heteroatomic or heterocyclic intersugar linkages. These include those having morpholino linkages (formed in part from the sugar portion of a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfone backbones; formacetyl and thioformacetyl backbones; methylene formacetyl and thioformacetyl backbones; alkene containing backbones; sulfamate backbones; methyleneimino and methylenehydrazino backbones; sulfonate and sulfonamide backbones; amide backbones; and others having mixed N, O, S and CH ₂ component parts.

Representative U.S. patents that teach the preparation of the above oligonucleotides include, but are not limited to, U.S. Pat. Nos. 5,034,506; 5, 166,315; 5,185,444; 5,214, 134; 5,216,141;

5,235,033; 5,264,562; 5,264,564; 5,405,938; 5,434,257; 5,466,677; 5,470,967; 5,489,677; 5,541,307; 5,561,225; 5,596,086; 5,602,240; 5,610,289; 5,602,240; 5,608,046; 5,610,289; 5,618,704; 5,623,070; 5,663,312; 5,633,360; 5,677,437; and 5,677,439, each of which is herein incorporated by reference.

In other examples of oligonucleotide mimetics, both the sugar and the internucleoside linkage, i.e., the backbone, of the nucleoside units may be replaced with other groups. The nucleobase units are maintained for hybridization with an appropriate nucleic acid target compound. One such oligonucleotide, an oligonucleotide mimetic, that has been shown to have excellent hybridization properties, is referred to as a peptide nucleic acid (PNA). In PNA compounds, the sugar-backbone of an oligonucleotide is replaced with an amide-containing backbone, in particular an aminoethylglycine backbone. The nucleobases are retained and are bound directly or indirectly to atoms of the amide portion of the backbone. Representative U.S. patents that teach the preparation of PNA compounds include, but are not limited to, U.S. Pat. Nos. 5,539,082; 5,714,331; and 5,719,262, each of which is herein incorporated by reference. Further teaching of PNA compounds can be found in Nielsen et al., Science, 1991, 254, 1497.

Also included are oligonucleotides that employ ribozymes. Synthetic RNA molecules and derivatives thereof that catalyze highly specific endoribonuclease activities are known as ribozymes. (See, generally, U.S. Pat. No. 5,543,508 to Haseloff et al., and U.S. Pat. No. 5,545,729 to Goodchild et al.). The cleavage reactions are catalyzed by the RNA molecules themselves. In naturally occurring RNA molecules, the sites of self -catalyzed cleavage are located within highly conserved regions of RNA secondary structure (Buzayan et al., PNAS USA. 83:8859, 1986). Naturally occurring autocatalytic RNA molecules have been modified to generate ribozymes which can be targeted to a particular cellular or pathogenic RNA molecule with a high degree of specificity. Thus, ribozymes serve the same general purpose as antisense oligonucleotides (i.e., modulation of expression of a specific gene) and, like oligonucleotides, are nucleic acids possessing significant portions of single- strandedness. That is, ribozymes have substantial chemical and functional identity with

oligonucleotides and are thus considered to be equivalents for purposes described herein.

In certain instances, the RNAi agents may be modified by non-ligand group. A number of non-ligand molecules have been conjugated to oligonucleotides in order to enhance the activity, cellular distribution, cellular targeting, or cellular uptake of the oligonucleotide, and procedures for performing such conjugations are available in the literature. Examples of non-ligand moieties include lipid moieties, such as cholesterol (Letsinger et al., Proc. Natl. Acad. Sci. USA, 1989, 86:6553), cholic acid (Manoharan et al., Bioorg. Med. Chem. Lett, 1994, 4:1053), a thioether, e.g., hexyl-5- tritylthiol (Manoharan et al., Ann. N.Y. Acad. Sci., 1992, 660:306; Manoharan et al., Bioorg. Med. Chem. Let., 1993, 3:2765), a thiocholesterol (Oberhauser et al., Nucl. Acids Res., 1992, 20:533), an aliphatic chain, e.g., dodecandiol or undecyl residues (Saison-Behmoaras et al., EMBO J., 1991, 10: 111; Kabanov et al., FEBS Lett, 1990, 259:327; Svinarchuk et al., Biochimie, 1993, 75:49), a phospholipid, e.g., di-hexadecyl-rac -glycerol or triethylammonium l,2-di-0-hexadecyl-rac-glycero-3- H-phosphonate (Manoharan et al., Tetrahedron Lett, 1995, 36:3651; Shea et al., Nucl. Acids Res., 1990, 18:3777), a polyamine or a polyethylene glycol chain (Manoharan et al., Nucleosides & Nucleotides, 1995, 14:969), or adamantane acetic acid (Manoharan et al., Tetrahedron Lett., 1995, 36:3651), a palmityl moiety (Mishra et al., Biochim. Biophys. Acta, 1995, 1264:229), or an octadecylamine or hexylamino-carbonyl-oxycholesterol moiety (Crooke et al., J. Pharmacol. Exp. Ther., 1996, 277:923). Typical conjugation protocols involve the synthesis of oligonucleotides bearing an aminolinker at one or more positions of the sequence. The amino group is then reacted with the molecule being conjugated using appropriate coupling or activating reagents. The conjugation reaction may be performed either with the oligonucleotide still bound to the solid support or following cleavage of the oligonucleotide in solution phase. Purification of the oligonucleotide conjugate by HPLC typically affords the pure conjugate.

Certain exemplary RNAi agents are provided for delivery in a vector. The term "vector" generally refers to a nucleic acid molecule, typically DNA, to which nucleic acid segments may be inserted and cloned, i.e., propagated. Hence, a vector will typically contain one or more unique restriction sites, and may be capable of autonomous replication in a defined host or vehicle organism such that the cloned sequence is reproducible. Vectors may include, without limitation, plasmids, phagemids, bacteriophages, bacteriophage-derived vectors, PAC, BAC, linear nucleic acids, e.g., linear DNA, viral vectors, etc., as appropriate. Expression vectors are generally configured to allow for and/or effect the expression of nucleic acids or ORFs introduced thereto in a desired expression system, e.g., in vitro, in a host cell, host organ and/or host organism. For example, expression vectors may advantageously comprise suitable regulatory sequences.

Exemplary vectors for use herein include viral vectors, which are well known and include vectors derived from for example, but without limitation, retroviruses, vaccinia viruses, poxviruses, adenoviruses, and adeno-associated viruses (AAV). Such viral vectors may me be engineered by recombinant techniques as known per se to introduce thereto nucleic acid sequence(s) encoding any one of the antisense or RNAi agents disclosed herein.

For example, a retroviral vector may be used herein to deliver an RNAi agent. Generally, retroviral vectors may comprise the retroviral genomic sequences encoding components necessary for the integration of the recombinant viral genome (randomly) into the host cell genome and the nucleic acid sequence(s) of interest, such as in particular the nucleic acid sequence(s) encoding any one of the antisense or RNAi agents disclosed herein. Such retroviral vectors may be readily constructed using standard recombinant techniques (e.g., Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, 1989) from a wide variety of retroviruses, including for example, B, C, and D type retroviruses as well as spumaviruses and lentiviruses (see RNA Tumor Viruses, Second Edition, Cold Spring Harbor Laboratory, 1985).

Recombinant adenoviral vectors may also be contemplated for delivery and expression of RNAi agents as disclosed herein in a host cell. Adenovirus-based viral vectors have the advantage of being capable of infecting non-dividing host cells, but the recombinant viral genome is not integrated into the host cell genome. For example, a suitable adenoviral vector, a method for constructing a recombinant adenoviral vector thereof, and a method for delivering the recombinant vector into host cells, are described in Xia H et al. (2002) (Nat. Biotech. 20: 1006-1010). Use of recombinant AAV (RAAV) vectors is also contemplated herein. RAAV vectors can infect both dividing and non- dividing cells and may incorporate its recombinant viral genome into that of the host cell. RAAV vectors may be generated from a variety of adeno-associated viruses, including for example, serotypes 1 through 6. Generally, RAAV vectors may comprise, in order, a 5' adeno-associated virus inverted terminal repeat (ITR), a nucleic acid of interest, such as in particular a nucleic acid sequence encoding any one of the antisense or RNAi agents disclosed herein, operatively linked to a sequence which regulates its expression in a host cell or host organism, and a 3' adeno-associated virus ITR. In addition, the rAAV vector may preferably have a polyadenylation signal. Suitable RAAV vectors are described inter alia in WO 1994/13788, WO 1993/24641, and in Goyenvalle et al. 2004 (Science 306: 1796-1799) where antisense sequences are linked to a modified U7 small nuclear RNA.

Other exemplary viral vectors for use herein are vectors derived from a pox virus such as a vaccinia virus, for example an attenuated vaccinia virus such as Modified Virus Ankara (MVA) or NYVAC, an avipox virus such as fowl pox virus or canary pox virus.

Additional examples of modulating agents, such as RNAi oligonucleotides including siRNA oligonucleotides, may be found in U.S. Application Publication Nos. 2009/0312531; 2009/0318676; 2011/0117125; 2011/0269814; 2007/0275465, 2007/0054279; 2006/0287260; 2006/0035254; and 2006/0008822, which are incorporated by reference.

Methods of Use

Certain embodiments include the use of the HRS polypeptides/expressible polynucleotides and compositions described herein for treating lung inflammation, either alone or in combination with immunotherapy agents. Included is the treatment of interstitial lung diseases (ILDs) and related disorders. In some embodiments, the HRS polypeptides/expressible polynucleotides and

compositions, methods, and/or combination therapies are employed to reduce lung inflammation, treat one or more ILDs, and or improve clinical symptoms or parameters of the disease in a subject in need thereof.

Thus, some embodiments include methods of treating lung inflammation in a subject in need thereof, comprising administering to the subject a histidyl-tRNA synthetase (HRS) polypeptide (for example, an HRS-Fc fusion polypeptide), or an expressible polynucleotide that encodes the HRS polypeptide.

Certain embodiments include methods of treating lung inflammation in a subject in need thereof, comprising administering to the subject (a) a histidyl-tRNA synthetase (HRS) polypeptide, or an expressible polynucleotide that encodes the HRS polypeptide; and (b) an immunomodulatory agent, for example, as described herein. In some embodiments, (a) and (b) are administered separately, and are optionally defined as described herein. In certain embodiments, (a) and (b) are administered together, optionally as a therapeutic composition described herein.

In particular embodiments, the immunomodulatory agent is pirfenidone or nintedanib. In some methods or compositions, the pirfenidone is administered at an individual dosage unit that ranges from about 50 to about 1000 mg, or an individual dosage unit of about no more than about, or at least about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, or 1000 mg, for example, in about 1, 2, or 3 capsules for oral dosing.

In some embodiments, the pirfenidone is administered at a daily dosage unit that ranges from about 100 to about 4000 mg/day, or a daily dosage unit of about, no more than about, or at least about 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 3100, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, or 4000 mg/day, for example, in about 1, 2, 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing.

In specific embodiments, the pirfenidone is administered at an individual dosage unit of about 800 mg (e.g., 801 mg), for example, as three -267 mg capsules for oral dosing, taken as three capsules per individual dosage, or the pirfenidone is administered at daily dosage unit of about 2400 mg/day (e.g., 2403 mg/day), for example, as nine -267 mg capsules for oral dosing three times daily, taken as three capsules per individual dosage.

In some methods or compositions, the nintedanib is administered at an individual dosage unit that ranges from about 10 to about 500 mg, or an individual dosage unit of about, no more than about, or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, or 500 mg, for example, in about 1, 2, or 3 capsules.

In some embodiments, the nintedanib is administered at a daily dosage unit that ranges from about 20 to about 1000 mg/day, or a daily dosage unit of about, no more than about, or at least about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000 mg/day, for example, in about 1, 2, 3, 4, 5, or 6 capsules.

In specific embodiments, the nintedanib is administered at a daily dosage unit that ranges from about 100 to 150 mg, or ranges from about 200 to 300 mg/day, for example, for a once or twice daily dosage, or the nintedanib is administered at a daily dosage unit of about 100 or 150 mg, or about 200 to 300 mg/day, for example, for a once or twice daily dosage.

In some embodiments, the immunomodulatory agent alters or improves one or more pharmacokinetic characteristics of the HRS polypeptide relative to the HRS polypeptide alone.

Examples of pharmacokinetic characteristics include stability or half-life, bioavailability (the fraction of a drug that is absorbed), tissue distribution, volume of distribution (apparent volume in which a drug is distributed immediately after it has been injected intravenously and equilibrated between plasma and the surrounding tissues), concentration (initial or steady -state concentration of drug in plasma), elimination rate constant (rate at which drugs are removed from the body), elimination rate (rate of infusion required to balance elimination), area under the curve of a concentration-time graph (AUC or exposure; integral of the concentration-time curve, after a single dose or in steady state), clearance (volume of plasma cleared of the drug per unit time), C _ma x (peak plasma concentration of a drug after oral administration), (time to reach Cmax), Cmin (lowest concentration that a drug reaches before the next dose is administered), and fluctuation (peak trough fluctuation within one dosing interval at steady state). In specific embodiments, the one or more altered pharmacokinetic characteristics of the HRS polypeptide are increased serum half -life, increased bioavailability, increased exposure (AUC), increased serum concentration, and/or decreased clearance.

In some embodiments, the immunomodulatory agent increases the half -life or serum half-life of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone. In some embodiments, the immunomodulatory agent increases the bioavailability of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone. In some embodiments, the immunomodulatory agent increases the exposure (AUC) of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone. In some embodiments, the immunomodulatory agent increases the serum concentration of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone. In some embodiments, the immunomodulatory agent decreases the clearance of the HRS polypeptide in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone. In particular embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or HRS ^FC1 ), and the immunomodulatory agent is pirfenidone. In specific embodiments, the pirfenidone increases the serum concentration of the HRS polypeptide (for example, HRS ^FC1 ) in the subject by at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200% or more relative to the HRS polypeptide alone.

Certain embodiments therefore include methods of of altering one or more pharmacokinetic characteristics of an HRS-Fc fusion polypeptide in a subject, comprising administering to the subject the HRS-Fc fusion polypeptide, or an expressible polynucleotide that encodes the HRS-Fc fusion polypeptide, in combination with pirfenidone. In particular embodiments, the HRS-Fc fusion polypeptide comprises, consists, or consists essentially of an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% identical to a sequence selected from Table H8. In specific embodiments, the HRS polypeptide comprises, consists, or consists essentially of SEQ ID NO: 157 (Fc-HRS(2-60) or HRS ^FC1 ).

In certain embodiments, the subject in need thereof has or is risk for having an inflammatory lung disease or an interstitial lung disease. Interstitial lung disease (ILD) refers to a broad category of lung diseases that includes more than 130 disorders characterized by scarring (i.e., "fibrosis") and/or inflammation of the lungs. ILD accounts for 15 percent of the cases seen by pulmonologists. In some instances, ILD is idiopathic. However, in some instances, ILD can develop from or associate with a variety of sources or conditions, ranging from other diseases to environmental factors. Some of the causes of, or associations with, ILD include, for example, connective tissue diseases, autoimmune disease, including for example, scleroderma/progressive systemic sclerosis, Lupus (systemic lupus erythematosus), rheumatoid arthritis, and polymyositis/dermatomyositis. Also included are occupational and environmental exposures, including for example, exposure to inhaled substances such as dust, gases, allergens, or toxins/poisons; exposure to drugs such as antibiotics,

chemotherapeutic agents, antiarrhythmic agents, and statins; exposure to infections such as pneumonia (e.g., atypical pneumonia), Pneumocystis pneumonia (PCP), tuberculosis, Chlamydia trachomatis, Respiratory Syncytial Virus (RSV), and cryptogenic organizing pneumonia; exposure to radiation therapy; and malignancies such as lymphangitic carcinomatosis and lymphoma. In certain embodiments, the subject in need thereof has acute respiratory distress syndrome (ARDS).

In ILD, the tissue in the lungs becomes inflamed and/or scarred. The interstitium of the lung includes the area in and around the small blood vessels and alveoli (air sacs) where the exchange of oxygen and carbon dioxide takes place. Inflammation and scarring of the interstitium disrupts this tissue and leads to a decrease in the ability of the lungs to extract oxygen from the air.

The progression of ILD varies from disease to disease and from person to person. Because interstitial lung disease disrupts the transfer of oxygen and carbon dioxide in the lungs, its symptoms typically manifest as problems with breathing. The two most common symptoms of ILD are shortness of breath with exercise and a non-productive cough.

In certain embodiments, the ILD is selected from, or is associated with, one or more of idiopathic interstitial pneumonia, idiopathic pulmonary fibrosis, sarcoidosis, Hammann-Rich syndrome, Antisynthetase syndrome, idiopathic eosinophilic pneumonia, alveolar hemorrhage syndrome, pulmonary alveolar proteinosis, asbestosis, silicosis, berylliosis, rheumatoid arthritis, lupus erythematosus, chronic graft vs host disease with pulmonary involvement, sclerosis (systemic) or scleroderma, polymyositis, dermatomyositis, chronic pulmonary disease, asthma, bronchitis (respiratory bronchitis), pneumonia, hypersensitivity pneumonitis, chronic hypersensitivity pneumonia, respiratory distress syndrome, Still's disease, acute lung injury, microscopic polyangitis, pulmonary edema, pulmonary Langerhans cell histiocytosis, acute inhalational exposures, drug- induced lung disease, desquamative interstitial pneumonia, and/or cystic fibrosis.

Certain genetic deficiencies or mutations also associate with ILDs. For example, in certain embodiments, the ILD is associated with one or more of Surfactant-Protein-B Deficiency (Mutations in SFTPB), Surfactant-Protein-C Deficiency (Mutations in SFTPC), ABCA3 -Deficiency (Mutations in ABCA3), Brain Lung Thyroid Syndrome (Mutations in TTF1), or Congenital Pulmonary Alveolar Proteinosis (Mutations in CSFR2A, CSFR2B), Alveolar Capillary Dysplasia (Mutations in FoxFl), Mutations in telomerase reverse transcriptase (TERT), Mutations in telomerase RNA component (TERC), Mutations in the regulator of telomere elongation helicase 1 (RTEL1), and/or Mutations in poly(A)-specific ribonuclease (PARN).

In some embodiments, the subject in need thereof has a condition selected from one or more of atopic asthma, non-atopic asthma, allergic asthma, atopic bronchial IgE-mediated asthma, bronchial asthma, essential asthma, true asthma, intrinsic asthma caused by pathophysiologic disturbances, extrinsic asthma caused by environmental factors, essential asthma of unknown or inapparent cause, non-atopic asthma, bronchitic asthma, emphysematous asthma, exercise-induced asthma, allergen induced asthma, cold air induced asthma, occupational asthma, infective asthma caused by bacterial, fungal, protozoal, or viral infection, non-allergic asthma, incipient asthma, wheezy infant syndrome and bronchiolytis, chronic or acute bronchoconstriction, chronic bronchitis, small airways obstruction, and emphysema.

In some embodiments, the subject in need thereof has an obstructive or inflammatory airway disease. Examples include chronic eosinophilic pneumonia, chronic obstructive pulmonary disease (COPD), COPD that includes chronic bronchitis, pulmonary emphysema or dyspnea, COPD that is characterized by irreversible, progressive airways obstruction, and acute respiratory distress syndrome (ARDS). In some embodiments, the subject in need thereof has a condition related to exacerbation of airways hyper-reactivity consequent to other drug therapy, airway disease that is associated with pulmonary hypertension, bronchitis or acute bronchitis, acute laryngotracheal bronchitis, arachidic bronchitis, catarrhal bronchitis, croupus bronchitis, dry bronchitis, infectious asthmatic bronchitis, productive bronchitis, staphylococcus or streptococcal bronchitis, vesicular bronchitis, acute lung injury, bronchiectasis or cylindric bronchiectasis, sacculated bronchiectasis, fusiform bronchiectasis, capillary bronchiectasis, cystic bronchiectasis, dry bronchiectasis, or follicular bronchiectasis.

In certain embodiments, as noted herein, the subject in need thereof has fibrosis, or pulmonary fibrosis. The severity of pulmonary fibrosis can be measured on a numerical scale, referred to as an Ashcroft score (see, e.g., Ashcroft et al., J. Clin. Path. 41:467-470, 1988). The Ashcroft score can range from minimal fibrous thickening of the alveolar or bronchioloar walls, moderate thickening of walls without obvious to lung architecture, increased fibrosis with definite damage to lung structure and formation of fibrous bands or small fibrous masses, severe distortion of structure and large fibrous areas (including "honeycomb lung"), and total fibrous obliteration of the field. An Ashcroft score of 0 refers to normal lung. In some embodiments, for example, prior to treatment, the subject in need thereof has an Ashcroft score of 1, 2, 3, 4, 5, 6, 7, or 8 (1 being the best and 8 being the worst score on the scale).

In certain embodiments, the lung inflammation has an autoimmune component. For instance, lung and peripheral blood T cells in patients with severe emphysema secrete Thl cytokines and chemokines when stimulated with elastin peptides in vitro, and these patients have increased anti- elastin antibody as compared to controls (see Goswami et al, The Journal of Immunology. 178:

130.41, 2007). Also, IgG autoantibodies with avidity for pulmonary epithelium, and the potential to mediate cytotoxicity, are prevalent in patients with COPD (see Feghali-Bostwick et al., Am J Respir Crit Care Med. 177: 156-63, 2008). Since autoreactive immune responses may be important in the etiology of this disease, including, for example, auto-reactive responses to self-antigens such as elastin, may play a role in COPD, the combination therapies described herein can be used to desensitize immune cells to these antigens and thereby reduce pulmonary inflammation.

In some embodiments, the methods and compositions increase the life expectancy of the subject in need thereof, for example, by about or at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60 or more years.

In some embodiments, the methods and compositions improve one or more of the clinical symptoms or parameters of the lung inflammation in the subject in need thereof. Exemplary clinical symptoms or parameters include lung fibrosis, inflammatory cell infiltrates in the lung, respiratory function, and body weight.

Thus, in some embodiments, the methods and compositions improve lung fibrosis in the subject in need thereof by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, for example, as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more. Particular embodiments improve lung fibrosis in the subject in need thereof as measured by a reduced Ashcroft score, for example, an Ashcroft score that is reduced by 1, 2, 3, 4, 5, 6, 7, or 8 grades relative to an earlier score. In some embodiments, the methods and compositions result in an Ashcroft score of 0, 1, 2, or 3 or less.

In some embodiments, the methods and compositions reduce inflammatory cell infiltrates in the lung by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, for example, as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more.

In some embodiments, the methods and compositions improve respiratory function by about or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000% or more, for example, as measured over a period of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 months or more. In some embodiments, respiratory function is selected from one or more of increased expiration time, increased inspiration time, decreased peak expiratory flow, decreased peak inspiratory flow, decreased respiratory minute volume (RMV), and decreased respiratory rate. Exemplary methods for measuring such symptoms or parameters are known in the art or described herein (see the Examples).

Pharmaceutical Compositions and Kits

Certain embodiments include pharmaceutical compositions, therapeutic compositions, and formulations suitable for the therapeutic delivery of the HRS polypeptides/expressible

polynucleotides and immunomodulatory agents described herein. Some embodiments therefore include pharmaceutically acceptable compositions that comprise a therapeutically -effective amount of one or more of the HRS polypeptides/expressible polynucleotides and immunomodulatory agents described herein, formulated together with one or more pharmaceutically acceptable carriers and/or diluents.

The compositions may be specially formulated for administration in solid or liquid form, including those adapted for the following: (1) oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, e.g., those targeted for buccal, sublingual, and systemic absorption, boluses, powders, granules, pastes for application to the tongue; (2) parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; (3) topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin; (4) intravaginally or intrarectally, for example, as a pessary, cream or foam; (5) sublingually ; (6) ocularly; (7) transdermally; or (8) nasally.

The phrase "pharmaceutically acceptable" is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

The phrase "pharmaceutically -acceptable carrier" as used herein means a pharmaceutically - acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, manufacturing aid (e.g., lubricant, talc magnesium, calcium or zinc stearate, or steric acid), or solvent encapsulating material, involved in carrying or transporting the subject compound from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not injurious to the subject.

Some examples of materials that can serve as pharmaceutically -acceptable carriers include, without limitation: (1) sugars, such as lactose, glucose and sucrose; (2) starches, such as corn starch and potato starch; (3) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; (4) powdered tragacanth; (5) malt; (6) gelatin; (7) talc; (8) excipients, such as cocoa butter and suppository waxes; (9) oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; (10) glycols, such as propylene glycol; (11) polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; (12) esters, such as ethyl oleate and ethyl laurate; (13) agar; (14) buffering agents, such as magnesium hydroxide and aluminum hydroxide; (15) alginic acid; (16) pyrogen-free water; (17) isotonic saline; (18) Ringer's solution; (19) ethyl alcohol; (20) pH buffered solutions; (21) polyesters, polycarbonates and/or poly anhydrides; and (22) other non-toxic compatible substances employed in pharmaceutical formulations.

Additional non-limiting examples of agents suitable for formulation with the HRS polypeptides/expressible polynucleotides and immunomodulatory agents include: PEG conjugated nucleic acids, phospholipid conjugated nucleic acids, nucleic acids containing lipophilic moieties, phosphorothioates, P-glycoprotein inhibitors (such as Pluronic P85) which can enhance entry of drugs into various tissues; biodegradable polymers, such as poly (DL-lactide-coglycolide) microspheres for sustained release delivery after implantation (Emerich, D F et al., 1999, Cell Transplant, 8, 47-58) Alkermes, Inc. Cambridge, Mass.; and loaded nanoparticles, such as those made of

polybutylcyanoacrylate, which can deliver drugs across the blood brain barrier and can alter neuronal uptake mechanisms (Prog Neuropsychopharmacol Biol Psychiatry, 23, 941-949, 1999).

Also included are compositions comprising surface-modified liposomes containing poly (ethylene glycol) lipids (PEG-modified, branched and unbranched or combinations thereof, or long- circulating liposomes or stealth liposomes). HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents can also comprise covalently attached PEG molecules of various molecular weights. These formulations offer a method for increasing the accumulation of drugs in target tissues. Long-circulating liposomes are also likely to protect drugs from nuclease degradation to a greater extent compared to cationic liposomes, based on their ability to avoid accumulation in metabolically aggressive MPS tissues such as the liver and spleen.

Also included are compositions prepared for delivery as described in U.S. Pat. Nos.

6,692,911, 7,163,695 and 7,070,807. In this regard, certain embodiments include compositions comprising copolymers of lysine and histidine (HK) as described in U.S. Pat. Nos. 7,163,695, 7,070,807, and 6,692,911 either alone or in combination with PEG (e.g., branched or unbranched PEG or a mixture of both), in combination with PEG and a targeting moiety or any of the foregoing in combination with a crosslinking agent. Some embodiments provide HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents in compositions comprising gluconic -acid- modified polyhistidine or gluconylated-polyhistidine/transferrin-poly lysine. One skilled in the art will also recognize that amino acids with properties similar to His and Lys may be substituted within the composition.

Certain agents described herein may contain a basic functional group, such as amino or alkylamino, which is capable of forming pharmaceutically -acceptable salts with pharmaceutically - acceptable acids. The term "pharmaceutically -acceptable salts" in this respect, refers to the relatively non-toxic, inorganic and organic acid addition salts of an agent. These salts can be prepared in situ in the administration vehicle or the dosage form manufacturing process, or by separately reacting a purified agent in its free base form with a suitable organic or inorganic acid, and isolating the salt thus formed during subsequent purification. Representative salts include the hydrobromide, hydrochloride, sulfate, bisulfate, phosphate, nitrate, acetate, valerate, oleate, palmitate, stearate, laurate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, napthylate, mesylate, glucoheptonate, lactobionate, and laurylsulphonate salts and the like.

The pharmaceutically acceptable salts of the agents described herein include the conventional nontoxic salts or quaternary ammonium salts of the compounds, e.g., from non-toxic organic or inorganic acids. For example, such conventional nontoxic salts include those derived from inorganic acids such as hydrochloride, hydrobromic, sulfuric, sulfamic, phosphoric, nitric, and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, palmitic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicyclic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methane sulfonic, ethane disulfonic, oxalic, isothionic, and the like.

In certain embodiments, the agents described herein contain one or more acidic functional groups and, thus, are capable of forming pharmaceutically acceptable salts with pharmaceutically acceptable bases. The term "pharmaceutically -acceptable salts" in these instances refers to the relatively non-toxic, inorganic and organic base addition salts of an agent. These salts can likewise be prepared in situ in the administration vehicle or the dosage form manufacturing process, or by separately reacting the purified compound in its free acid form with a suitable base, such as the hydroxide, carbonate or bicarbonate of a pharmaceutically -acceptable metal cation, with ammonia, or with a pharmaceutically -acceptable organic primary, secondary or tertiary amine. Representative alkali or alkaline earth salts include the lithium, sodium, potassium, calcium, magnesium, and aluminum salts and the like. Representative organic amines useful for the formation of base addition salts include ethylamine, diethylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine and the like.

Wetting agents, emulsifiers and lubricants, such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions. Examples of pharmaceutically -acceptable antioxidants include: (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.

Formulations of include those suitable for intravenous, intramuscular, oral, nasal, pulmonary, topical (including buccal and sublingual), rectal, vaginal, and/or parenteral administration. The formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharmacy. The amount of active ingredient that can be combined with a carrier material to produce a single dosage form will vary depending upon the host being treated, the particular mode of administration. The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will generally be that amount of the compound which produces a therapeutic effect. Generally, out of one hundred percent, this amount will range from about 0.1 percent to about ninety -nine percent of active ingredient, preferably from about 5 percent to about 70 percent, most preferably from about 10 percent to about 30 percent.

In certain embodiments, a composition or formulation comprises an excipient selected from cyclodextrins, celluloses, liposomes, micelle forming agents, e.g., bile acids, and polymeric carriers, e.g., polyesters and polyanhydrides; and an HRS polypeptide/expressible polynucleotide and immunomodulatory agent.

Formulations suitable for oral administration may be in the form of capsules, cachets, pills, tablets, lozenges (using a flavored basis, usually sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in- oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of an HRS polypeptide/expressible polynucleotide and/or immunomodulatory agent as an active ingredient. The compositions or agents may also be administered as a bolus, electuary, or paste.

In solid dosage forms for oral administration (capsules, tablets, pills, dragees, powders, granules, trouches and the like), the active ingredient may be mixed with one or more

pharmaceutically -acceptable carriers, such as sodium citrate or dicalcium phosphate, and/or any of the following: (1) fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; (2) binders, such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; (3) humectants, such as glycerol; (4) disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; (5) solution retarding agents, such as paraffin; (6) absorption accelerators, such as quaternary ammonium compounds and surfactants, such as poloxamer and sodium lauryl sulfate; (7) wetting agents, such as, for example, cetyl alcohol, glycerol monostearate, and non-ionic surfactants; (8) absorbents, such as kaolin and bentonite clay; (9) lubricants, such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, zinc stearate, sodium stearate, stearic acid, and mixtures thereof; (10) coloring agents; and (11) controlled release agents such as crospovidone or ethyl cellulose. In the case of capsules, tablets and pills, the compositions may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers in soft and hard-shelled gelatin capsules using such excipients as lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like.

A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared using binder (e.g., gelatin or hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose), surface -active or dispersing agent. Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.

The tablets and other solid dosage forms, such as dragees, capsules, pills and granules, may optionally be scored or prepared with coatings and shells, such as enteric coatings and other coatings well known in the pharmaceutical-formulating art. They may also be formulated so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile, other polymer matrices, liposomes and/or microspheres. They may be formulated for rapid release, e.g., freeze-dried. They may be sterilized by, for example, filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved in sterile water, or some other sterile injectable medium immediately before use. These compositions may also optionally contain opacifying agents and may be of a composition that they release the active ingredient(s) only, or preferentially, in a certain portion of the gastrointestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. The active ingredient can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients.

Liquid dosage forms for oral administration of the HRS polypeptides/expressible polynucleotides and immunomodulatory agents include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredient, the liquid dosage forms may contain inert diluents commonly used in the art, such as, for example, water or other solvents, solubilizing agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.

I l l Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming and preservative agents.

Suspensions, in addition to the active compounds, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters,

microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, and mixtures thereof.

Formulations or dosage forms for the topical or transdermal administration of the HRS polypeptides/expressible polynucleotides and immunomodulatory agents include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants. The active HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents may be mixed under sterile conditions with a pharmaceutically -acceptable carrier, and with any preservatives, buffers, or propellants which may be required. The ointments, pastes, creams and gels may contain, in addition to the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents, excipients, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.

Powders and sprays can contain excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances. Sprays can additionally contain customary propellants, such as chlorofluorohydrocarbons and volatile unsubstituted hydrocarbons, such as butane and propane.

Transdermal patches have the added advantage of providing controlled delivery of the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents to the body. Such dosage forms can be made by dissolving or dispersing the agent in the proper medium. Absorption enhancers can also be used to increase the flux of the agent across the skin. The rate of such flux can be controlled by either providing a rate controlling membrane or dispersing the agent in a polymer matrix or gel, among other methods known in the art.

Pharmaceutical compositions suitable for parenteral administration may comprise one or more HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents in combination with one or more pharmaceutically -acceptable sterile isotonic aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, or sterile powders which may be reconstituted into sterile injectable solutions or dispersions just prior to use, which may contain sugars, alcohols, antioxidants, buffers, bacteriostats, solutes which render the formulation isotonic with the blood of the intended recipient or suspending or thickening agents. Examples of suitable aqueous and nonaqueous carriers which may be employed in the pharmaceutical compositions include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.

These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of the action of microorganisms upon the subject may be ensured by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents which delay absorption such as aluminum monostearate and gelatin.

In some cases, in order to prolong the effect of a drug, it is desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material having poor water solubility, among other methods known in the art. The rate of absorption of the drug then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally -administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle.

Injectable depot forms may be made by forming microencapsule matrices of the subject HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents in biodegradable polymers such as polylactide-polyglycolide. Depending on the ratio of agent to polymer, and the nature of the particular polymer employed, the rate of release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations may also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissues.

When the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents are administered as pharmaceuticals, to humans and animals, they can be given per se or as a pharmaceutical composition containing, for example, 0.1 to 99% (more preferably, 10 to 30%) of active ingredient in combination with a pharmaceutically acceptable carrier.

The phrases "parenteral administration" and "administered parenterally" as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticulare, subcapsular, subarachnoid, intraspinal and intrasternal injection and infusion.

The phrases "systemic administration," "administered systemically," "peripheral administration" and "administered peripherally" as used herein mean the administration of a compound, drug or other material other than directly into the central nervous system, such that it enters the patient's system and, thus, is subject to metabolism and other like processes, for example, subcutaneous administration. Regardless of the route of administration selected, the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents may be formulated into pharmaceutically - acceptable dosage forms by conventional methods known to those of skill in the art. Actual dosage levels of the active ingredients in the pharmaceutical compositions may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being unacceptably toxic to the patient.

The selected dosage level will depend upon a variety of factors including the activity of the particular HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion or metabolism of the particular agent being employed, the rate and extent of absorption, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular agent employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts.

A physician having ordinary skill in the art can readily determine and prescribe the effective amount of the pharmaceutical composition required. For example, the physician could start doses of the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents employed in the pharmaceutical composition at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved. In general, a suitable daily dose of HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents will be that amount of the compound which is the lowest dose effective to produce a therapeutic effect. Such an effective dose will generally depend upon the factors described herein. Generally, oral, intravenous, intramuscular, intracerebroventricular and subcutaneous doses of the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents for a subject or patient, when used for the indicated effects, will range from about 0.0001 to about 100 mg per dosage, or about about 0.0001 to about 100 mg per kilogram of body weight per dosage.

If desired, the effective daily dose of the active agent(s) may be administered as one, two, three, four, five, six or more sub-doses administered separately at appropriate intervals throughout the day or week, for example, in unit dosage forms. In certain situations, dosing is one administration per day. In certain situations, dosing is one, two, or three administration per week. In certain

embodiments, dosing is one or more administration per every 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 days, or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 weeks, or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 months, as needed, to treat the desired condition.

HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents can be administered to cells by a variety of methods known to those familiar to the art, including, but not restricted to, encapsulation in liposomes, by iontophoresis, or by incorporation into other vehicles, such as hydrogels, cyclodextrins, biodegradable nanocapsules, and bioadhesive microspheres, as described herein and known in the art. In certain embodiments, microemulsification technology may be utilized to improve bioavailability of lipophilic (water insoluble) pharmaceutical agents. Examples include Trimetrine (Dordunoo, S. K., et al., Drug Development and Industrial Pharmacy, 17(12), 1685-1713, 1991 and REV 5901 (Sheen, P. C, et al., J Pharm Sci 80(7), 712-714, 1991). Among other benefits, microemulsification provides enhanced bioavailability by preferentially directing absorption to the lymphatic system instead of the circulatory system, which thereby bypasses the liver, and prevents destruction of the compounds in the hepatobiliary circulation.

In some embodiments, the compositions or formulations contain micelles which are formed from the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents and at least one amphiphilic carrier, in which the micelles have an average diameter of less than about 100 nm. Exemplary embodiments provide micelles having an average diameter less than about 50 nm, and even certain embodiments provide micelles having an average diameter less than about 30 nm, or even less than about 20 nm. While all suitable amphiphilic carriers are contemplated, the presently preferred carriers are generally those that have Generally -Recognized-as-Safe (GRAS) status, and that can both solubilize the active ingredient and microemulsify it at a later stage when the solution comes into a contact with a complex water phase (such as one found in human gastro-intestinal tract).

Usually, amphiphilic ingredients that satisfy these requirements have HLB (hydrophilic to lipophilic balance) values of 2-20, and their structures contain straight chain aliphatic radicals in the range of C- 6 to C-20. Examples are polyethylene-glycolized fatty glycerides and polyethylene glycols.

Examples of amphiphilic carriers include saturated and monounsaturated

polyethyleneglycolyzed fatty acid glycerides, such as those obtained from fully or partially hydrogenated various vegetable oils. Such oils may advantageously consist of tri-, di-, and mono-fatty acid glycerides and di- and mono-poly ethyleneglycol esters of the corresponding fatty acids, with a particularly preferred fatty acid composition including capric acid 4-10, capric acid 3-9, lauric acid 40-50, myristic acid 14-24, palmitic acid 4-14 and stearic acid 5-15%. Another useful class of amphiphilic carriers includes partially esterified sorbitan and/or sorbitol, with saturated or monounsaturated fatty acids (SPAN-series) or corresponding ethoxylated analogs (TWEEN-series).

Commercially available amphiphilic carriers may be particularly useful, including Gelucire- series, Labrafil, Labrasol, or Lauroglycol (all manufactured and distributed by Gattefosse

Corporation, Saint Priest, France), PEG-mono-oleate, PEG-di-oleate, PEG-mono-laurate and di- laurate, Lecithin, Polysorbate 80, etc. (produced and distributed by a number of companies in USA and worldwide).

In certain embodiments, the delivery may occur by use of liposomes, nanocapsules, microparticles, microspheres, lipid particles, vesicles, and the like, for the introduction of the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents into suitable host cells. In particular, the compositions may be formulated for delivery either encapsulated in a lipid particle, a liposome, a vesicle, a nanosphere, a nanoparticle or the like. The formulation and use of such delivery vehicles can be carried out using known and conventional techniques.

Hydrophilic polymers suitable for use are those which are readily water-soluble, can be covalently attached to a vesicle-forming lipid, and which are tolerated in vivo without toxic effects (i.e., are biocompatible). Suitable polymers include polyethylene glycol (PEG), polylactic (also termed polylactide), poly gly colic acid (also termed polyglycolide), a poly lactic -poly gly colic acid copolymer, and polyvinyl alcohol. In certain embodiments, polymers have a molecular weight of from about 100 or 120 daltons up to about 5,000 or 10,000 daltons, or from about 300 daltons to about 5,000 daltons. In other embodiments, the polymer is polyethyleneglycol having a molecular weight of from about 100 to about 5,000 daltons, or having a molecular weight of from about 300 to about 5,000 daltons. In certain embodiments, the polymer is polyethyleneglycol of 750 daltons (PEG(750)). Polymers may also be defined by the number of monomers therein.

Other hydrophilic polymers which may be suitable include polyvinylpyrrolidone, polymethoxazoline, polyethyloxazoline, polyhydroxypropyl methacrylamide, polymethacrylamide, polydimethylacrylamide, and derivatized celluloses such as hydroxymethylcellulose or

hydroxy ethylcellulose.

In certain embodiments, a composition or formulation comprises a biocompatible polymer selected from the group consisting of polyamides, polycarbonates, polyalkylenes, polymers of acrylic and methacrylic esters, polyvinyl polymers, polyglycolides, polysiloxanes, polyurethanes and copolymers thereof, celluloses, polypropylene, polyethylenes, polystyrene, polymers of lactic acid and glycolic acid, polyanhydrides, poly(ortho)esters, poly(butic acid), poly(valeric acid), poly(lactide-co- caprolactone), polysaccharides, proteins, polyhyaluronic acids, polycyanoacrylates, and blends, mixtures, or copolymers thereof.

Cyclodextrins are cyclic oligosaccharides, consisting of 6, 7 or 8 glucose units, designated by the Greek letter α, β, and γ, respectively. The glucose units are linked by a-l,4-glucosidic bonds. As a consequence of the chair conformation of the sugar units, all secondary hydroxyl groups (at C-2, C-3) are located on one side of the ring, while all the primary hydroxyl groups at C-6 are situated on the other side. As a result, the external faces are hydrophilic, making the cyclodextrins water-soluble. In contrast, the cavities of the cyclodextrins are hydrophobic, since they are lined by the hydrogen of atoms C-3 and C-5, and by ether-like oxygens. These matrices allow complexation with a variety of relatively hydrophobic compounds, including, for instance, steroid compounds such as 17a-estradiol (see, e.g., van Uden et al. Plant Cell Tiss. Org. Cult. 38: 1-3-113 (1994)). The complexation takes place by Van der Waals interactions and by hydrogen bond formation. The physico-chemical properties of the cyclodextrin derivatives depend on the kind and the degree of substitution. For example, their solubility in water ranges from insoluble (e.g., triacetyl-beta-cyclodextrin) to 147% soluble (w/v) (G-2-beta-cyclodextrin). In addition, they are soluble in many organic solvents. The properties of the cyclodextrins enable the control over solubility of various formulation components by increasing or decreasing their solubility.

Numerous cyclodextrins and methods for their preparation have been described. For example, Parmeter (I), et al. (U.S. Pat. No. 3,453,259) and Gramera, et al. (U.S. Pat. No. 3,459,731) described electroneutral cyclodextrins. Other derivatives include cyclodextrins with cationic properties

[Parmeter (II), U.S. Pat. No. 3,453,257], insoluble crosslinked cyclodextrins (Solms, U.S. Pat. No. 3,420,788), and cyclodextrins with anionic properties [Parmeter (III), U.S. Pat. No. 3,426,011].

Among the cyclodextrin derivatives with anionic properties, carboxylic acids, phosphorous acids, phosphinous acids, phosphonic acids, phosphoric acids, thiophosphonic acids, thiosulphinic acids, and sulfonic acids have been appended to the parent cyclodextrin [see, Parmeter (III), supra]. Furthermore, sulfoalkyl ether cyclodextrin derivatives have been described by Stella, et al. (U.S. Pat. No.

5,134,127).

Some embodiments relate to formulations comprising liposomes containing HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents, where the liposome membrane is formulated to provide a liposome with increased carrying capacity. Alternatively or in addition, the active ingredients may be contained within, or adsorbed onto, the liposome bilayer of the liposome. The HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents may be aggregated with a lipid surfactant and carried within the liposome's internal space; in these cases, the liposome membrane is formulated to resist the disruptive effects of the active agent-surfactant aggregate.

Liposomes consist of at least one lipid bilayer membrane enclosing an aqueous internal compartment. Liposomes may be characterized by membrane type and by size. Small unilamellar vesicles (SUVs) have a single membrane and typically range between 0.02 and 0.05 μπι in diameter; large unilamellar vesicles (LUVS) are typically larger than 0.05 μπι. Oligolamellar large vesicles and multilamellar vesicles have multiple, usually concentric, membrane layers and are typically larger than 0.1 μπι. Liposomes with several nonconcentric membranes, i.e., several smaller vesicles contained within a larger vesicle, are termed multivesicular vesicles.

In some embodiments, the lipid bilayer of a liposome contains lipids derivatized with polyethylene glycol (PEG), such that the PEG chains extend from the inner surface of the lipid bilayer into the interior space encapsulated by the liposome, and extend from the exterior of the lipid bilayer into the surrounding environment.

Liposomes maybe prepared by any of a variety of techniques that are known in the art. See, e.g., U.S. Pat. No. 4,235,871; Published PCT applications WO 96/14057; New RRC, Liposomes: A practical approach, IRL Press, Oxford (1990), pages 33-104; Lasic DD, Liposomes from physics to applications, Elsevier Science Publishers BV, Amsterdam, 1993. For example, liposomes may be prepared by diffusing a lipid derivatized with a hydrophilic polymer into preformed liposomes, such as by exposing preformed liposomes to micelles composed of lipid-grafted polymers, at lipid concentrations corresponding to the final mole percent of derivatized lipid which is desired in the liposome. Liposomes containing a hydrophilic polymer can also be formed by homogenization, lipid- field hydration, or extrusion techniques, as are known in the art.

In another exemplary formulation procedure, the HRS polypeptides/expressible

polynucleotides and/or immunomodulatory agents are first dispersed by sonication in a

lysophosphatidylcholine or other low CMC surfactant (including polymer grafted lipids) that readily solubilizes hydrophobic molecules. The resulting micellar suspension of active agent is then used to rehydrate a dried lipid sample that contains a suitable mole percent of polymer-grafted lipid, or cholesterol. The lipid and active agent suspension is then formed into liposomes using extrusion techniques as are known in the art, and the resulting liposomes separated from the unencapsulated solution by standard column separation.

In one aspect, the liposomes are prepared to have substantially homogeneous sizes in a selected size range. One effective sizing method involves extruding an aqueous suspension of the liposomes through a series of polycarbonate membranes having a selected uniform pore size; the pore size of the membrane will correspond roughly with the largest sizes of liposomes produced by extrusion through that membrane. See e.g., U.S. Pat. No. 4,737,323 (Apr. 12, 1988). In certain embodiments, reagents such as DharmaFECT™ and Lipofectamine™ may be utilized to introduce polynucleotides or proteins into cells.

The release characteristics of a formulation depend on the encapsulating material, the concentration of encapsulated drug, and the presence of release modifiers. For example, release can be manipulated to be pH dependent, for example, using a pH sensitive coating that releases only at a low pH, as in the stomach, or a higher pH, as in the intestine. An enteric coating can be used to prevent release from occurring until after passage through the stomach. Multiple coatings or mixtures of cyanamide encapsulated in different materials can be used to obtain an initial release in the stomach, followed by later release in the intestine. Release can also be manipulated by inclusion of salts or pore forming agents, which can increase water uptake or release of drug by diffusion from the capsule. Excipients which modify the solubility of the drug can also be used to control the release rate. Agents which enhance degradation of the matrix or release from the matrix can also be incorporated. They can be added to the drug, added as a separate phase (i.e., as particulates), or can be co-dissolved in the polymer phase depending on the compound. In most cases the amount should be between 0.1 and thirty percent (w/w polymer). Types of degradation enhancers include inorganic salts such as ammonium sulfate and ammonium chloride, organic acids such as citric acid, benzoic acid, and ascorbic acid, inorganic bases such as sodium carbonate, potassium carbonate, calcium carbonate, zinc carbonate, and zinc hydroxide, and organic bases such as protamine sulfate, spermine, choline, ethanolamine, diethanolamine, and triethanolamine and surfactants such as Tween™ and Pluronic™. Pore forming agents which add microstructure to the matrices (i.e., water soluble compounds such as inorganic salts and sugars) are added as particulates. The range is typically between one and thirty percent (w/w polymer).

Uptake can also be manipulated by altering residence time of the particles in the gut. This can be achieved, for example, by coating the particle with, or selecting as the encapsulating material, a mucosal adhesive polymer. Examples include most polymers with free carboxyl groups, such as chitosan, celluloses, and especially polyacrylates (as used herein, polyacrylates refers to polymers including acrylate groups and modified aery late groups such as cyanoacrylates and methacrylates).

The HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents may be formulated to be contained within, or, adapted to release by a surgical or medical device or implant. In certain aspects, an implant may be coated or otherwise treated with an agent. For example, hydrogels, or other polymers, such as biocompatible and/or biodegradable polymers, may be used to coat an implant with the HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents (i.e., the composition may be adapted for use with a medical device by using a hydrogel or other polymer). Polymers and copolymers for coating medical devices with an agent are well-known in the art. Examples of implants include, but are not limited to, stents, drug-eluting stents, sutures, prosthesis, vascular catheters, dialysis catheters, vascular grafts, prosthetic heart valves, cardiac pacemakers, implantable cardioverter defibrillators, IV needles, devices for bone setting and formation, such as pins, screws, plates, and other devices, and artificial tissue matrices for wound healing.

The HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents may be administered in any convenient vehicle which is physiologically acceptable. Such a composition may include any of a variety of standard pharmaceutically acceptable carriers employed by those of ordinary skill in the art. Examples include, but are not limited to, saline, phosphate buffered saline (PBS), water, aqueous ethanol, emulsions, such as oil/water emulsions or triglyceride emulsions, tablets and capsules. The choice of suitable physiologically acceptable carrier will vary dependent upon the chosen mode of administration.

Also included are kits, for example, patient care kits, comprising one or more containers filled with one or more of the therapeutic compositions, HRS polypeptides/expressible polynucleotides and/or immunomodulatory agents described herein. In some embodiments, the kits include written instructions on how to use such compositions, for example, in the treatment of one or more diseases.

Certain embodiments therefore include a patient care kit, comprising: (a) a histidyl-tRNA synthetase (HRS) polypeptide, or an expressible polynucleotide that encodes the HRS polypeptide; and (b) an immunomodulatory agent. In some kits, (a) and (b) are in separate compositions, and are optionally defined as described herein. In some kits, (a) and (b) are in the same composition, optionally as a therapeutic composition as described herein.

In particular embodiments, the immunomodulatory agent in a therapeutic composition and/or patient care kit is pirfenidone or nintedanib. In some embodiments, the pirfenidone is at an individual dosage unit that ranges from about 50 to about 1000 mg (optionally in about 1, 2, or 3 capsules for oral dosing), or an individual dosage unit of about no more than about, or at least about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, or 1000 mg (optionally in 1, 2, or 3 capsules for oral dosing).

In some embodiments, the pirfenidone is at a daily dosage unit that ranges from about 100 to about 4000 mg/day (optionally in about 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing), or a daily dosage unit of about, no more than about, or at least about 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 10, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 3100, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, or 4000 mg/day (optionally in about 3, 4, 5, 6, 7, 8, 9 capsules for oral dosing).

In specific embodiments, the pirfenidone is at an individual dosage unit of about 800 mg (e.g., 801 mg), for example, as three -267 mg capsules for oral dosing, taken as three capsules per individual dosage. In specific embodiments, the pirfenidone is at daily dosage unit of about 2400 mg/day (e.g., 2403 mg/day), for example, as nine -267 mg capsules for oral dosing three times daily, taken as three capsules per individual dosage.

In some embodiments, the nintedanib is at an individual dosage unit that ranges from about 10 to about 500 mg (optionally in about 1, 2, or 3 capsules), or an individual dosage unit of about, no more than about, or at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, or 500 mg (optionally in about 1, 2, or 3 capsules).

In some embodiments, the nintedanib is at a daily dosage unit that ranges from about 20 to about 1000 mg/day (optionally in about 1, 2, 3, 4, 5, or 6 capsules), or a daily dosage unit of about, no more than about, or at least about 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000 mg/day (optionally in about 1, 2, 3, 4, 5, or 6 capsules). In some embodiments, the nintedanib is at a daily dosage unit that ranges from about 100 to 150 mg, or ranges from about 200 to 300 mg/day, optionally for a once or twice daily dosage. In some embodiments, the nintedanib is at a dosage unit of about 100 or 150 mg, or about 200 to 300 mg/day, optionally for a once or twice daily dosage.

The kits and compositions described herein may also include a one or more additional therapeutic agents or other components suitable or desired for the indication being treated. An additional therapeutic agent may be contained in a second container, if desired. Examples of additional therapeutic agents include, but are not limited to anti-inflammatory agents, anticancer agents, antibacterial agents, antiviral agents, etc.

The kits herein can also include one or more syringes (e.g., injectable syringes) or other components necessary or desired to facilitate an intended mode of delivery (e.g., stents, implantable depots, etc.).

EXAMPLES

Histidyl-tRNA synthetase (HRS) polypeptides were tested for activity in a bleomycin-induced pulmonary fibrosis model in mice or rats. Intratracheal administration of bleomycin (BLM) induces sequential changes in lung similar to those of certain ILDs, including for example, lung inflammation, and fibrosis seen in various ILDs in humans, suggesting that it may be used as a general model for the investigation for the evaluation of therapeutic agents for the treatment of ILDs, pulmonary injury, inflammation and fibrosis (see, e.g., Adamson and Bowden. The American J. Path. 77: 185-197, 1974; Wynn, J Exp Med. 208: 1339-1350, 2011; Williamson et al., Exp. Lung Res. 41:57-73, 2015).

Example 1

Evaluation of HRS polypeptides for the Treatment of Bleomycin-induced Interstitial Lung

Disease

Studies were performed to determine if an exemplary HRS polypeptide (HisRS 1 ^N8 ) reduces respiratory impairment and/or lung fibrosis in a mouse model of bleomycin-induced the lung fibrosis. The effects of the HRS polypeptide on inflammatory and fibrotic processes were determined by measuring the cellular content of the bronchoalveolar lavage (BAL), histological fibrosis score, and lung collagen content.

Protocol and Methods. Lung inflammation and fibrosis were induced in male C57B1/6J mice by oropharyngeal administration of BLM. After the habituation phase mice were randomized based on body weight over the different groups. Under isoflurane anesthesia, 50 μΐ PBS or BLM was dripped onto the vocal cords facilitating aspiration. Mice were sacrificed at day 21 using a terminal bleed under isoflurane anesthesia. Animals were treated as shown in Table El below. Table El.

Day: 0 1 9 : 13 : 14 ; is ; 16 17 : 18 : 19 : control : - saline

untreated

Vehicle PO

Nintedanib 60 mg/kg PO Ψ ·- ! _\ ! : : \ : .· |

Dexamethasone 0.25 mg/kg PO

Vehicle IV

Test Article 1 mg/kg IV

Test Article 3 mg/kg IV

Test Article 10 mg/kg IV

Β # # # : # : # # : # : # : # # # : # : #

χ

Male C57bl/6 mice, monitor BW

Bleomycin 0.04 mg/mouse oropharyngeal admin.

Test Article lot # S0613043

D20 administration (new collagen measure) B = bolus I P; # = present in drinking water

X takedown - serum, BALF cell counts, frozen pellets, fluid, collagen content, lung histology, frozen lung

Animals receiving PBS on day 0 served as non-diseased controls. Animals receiving BLM oropharangeally on day 0 received either no therapeutic treatment, oral vehicle (0.5% Natrosol), oral Nintedanib (60 mg/kg in Natrosol), oral dexamethasone (0.25 mg/kg in water), intravenous (IV) vehicle (50 mM His, 140 mM NaCl, 0.05% polysorbate 20, pH 7.3), or Test Article (HisRSl ^N8 ; Lot# S0613043) diluted in the IV vehicle at 1, 3 or 10 mg/kg. Daily oral treatments commenced on Day 0, while daily IV treatments commenced on Day 8. Dosing was planned to continue until the day of termination (Day 21), however, due to high mortality in IV treated groups (see results), IV dosing was skipped on Day 19 and Day 20. In order to determine new collagen formation, mice were given an IP bolus injection of 100% deuterated (D20) water/ 0.9% NaCl (35μ1 / gram body weight) at day 7; one day before starting treatment with Test Article. Subsequently, the mice received 8% D20 (Kinemed, Emeryville) drinking water until sacrifice to maintain labeling of newly formed collagen. Body weight was monitored three times a week.

At termination, mice were sacrificed using isoflurane and exsanguination via heart puncture one day after the last dosing (non-diseased controls, dexamethasone and vehicle groups) or 2 hours after the last IV administration of Test Article. Blood samples were processed to plasma (new collagen assay) or serum and stored frozen (< -70°C).

The lungs were flushed two times with 750 μΐ PBS, combined into one bronchoalveolar lavage (BAL) sample, and stored on ice until centrifugation. The supernatant was collected and stored frozen (< -70°C). The erythrocytes in the cell pellet were lysed and the remaining cells were resuspended in 100 μΐ PBS. 20 μΐ was diluted in trypan blue solution and used for counting the live cells using a hemocytometer.

The five lung lobes were collected and isolated. Wet weight of the medial and accessory lung lobes was recorded. The lobes were then snap frozen and stored at < -70°C until use for

hydroxyproline analysis. The left lung lobe was inflated and fixed for 48 hours in 4% formaldehyde and embedded in paraffin for histological analysis. The cranial lung lobe was snap frozen and stored at -80°C until shipment to Kinemed for the determination of new collagen formation.

Sections of the left lung lobe were stained using Masson's Trichrome and were scored in a blinded fashion using a modified Ashcroft score (Hubner et al., BioTechniques. 44:507-511, 514-507, 2008). Hydroxyproline content was determined in the medial and accessory lung lobes using acid hydrolysis followed by a chromogenic assay (QuickZyme Biosciences, Leiden, Netherlands). Newly formed collagen was measured in the cranial lung lobe by Kinemed Inc., Emeryville, USA using the fraction of body water enriched in 2H for normalization.

All statistical analyses were performed using the SPSS 22 for Windows statistical software package (SPPS Inc., Chicago, IL, USA). First, the equality of error variances was tested using Levene's test; equal variances were assumed at p>0.01. In the case of equality of error variances, groups were compared using the one-way ANOVA test, followed, when appropriate by a post-hoc Dunnett test versus the BLM + appropriate vehicle group. In the case of inequality of error variances, groups were compared using the Kruskall-Wallis test, followed by a Mann-Whitney test.

Results - Survival. The study commenced with 7 animals in the non-diseased control group and 12 animals in the BLM-induced groups. Survival is show in Table E2 below.

All animals in the non-diseased control (saline IT) group and in the BLM-treated PO-vehicle group survived the experimental period. The survival rate in the BLM untreated group was 83%. In the oral treated groups the survival rates were 75% after dexamethasone treatment and 92%

Nintedanib treatment group. Survival rates dropped to 58% in the IV Vehicle and to a similar range in the Test Article treated groups (see Table E2).

Results - Body Weights. Based on data from animals which survived to scheduled necropsy, administration of BLM in the lungs resulted in a decrease of body weight of the mice during the initial phase (first eight to ten days) of the experiment which is a characteristic of this model. After this phase the body weights of BLM-induced mice stabilized and started to increase slowly over the remaining experimental period. In the PBS-induced non-treated group, body weights remained stable and slowly increased (in total 6%) over the entire experimental period. When animals are treated orally on daily basis with vehicle, dexamethasone, or Nintedanib the body weights were comparable to untreated BLM-induced animals and stayed around 90% of their initial body weights. Until start of the IV treatment, body weight was comparable in all IV groups. After starting IV treatment with Test Article at day 8, body weights of the 1 and 3 mg/kg treatment dropped below or followed the trend line of the BLM untreated group. Daily IV treatment with the Test Article at 10 mg/kg had a positive effect on the BW when compared to the lower dose groups.

Results - Cell Counts in BAL Fluid. The total number of cells in the BAL per sample was 19.8 times higher after induction of lung fibrosis by BLM (see Figure 1). Oral treatment with dexamethasone or Nintedanib did not significantly affect live cells counted in BAL fluid at termination. In the Test Article -treated groups the cell number in BAL fluid compared with the IV- vehicle treated group was reduced by about 46%, 38% and 45% after IV treatment with Test Article at 1, 3 or 10 mg/kg, respectively.

Results - Lung mass, collagen content and new collagen content. BLM induction significantly affected the lung wet and dry weight of the BLM-induced non-treated mice resulting in a 1.8-fold increase in wet weight and a 2.1-fold increase in lung dry weight compared to PBS-induced mice. Dexamethasone decreased lung wet weight 15.6% when compared to the PO vehicle treated group. No significant changes in lung wet or dry weight were observed with other treatments.

Induction with BLM resulted in a significant 1.97-fold increase in collagen content in the medial and accessory lung lobes compared to PBS-induced mice. No significant changes in collagen content in the medial and accessory lung lobes were observed with any treatment.

A significant, 5.4-fold increase in new collagen synthesis after BLM administration was observed compared to mice receiving PBS IT. No significant changes in new collagen synthesis were observed with any treatment.

Results - Histological Fibrosis (Ashcroft) Score. The extent of fibrosis in the lungs was determined by Ashcroft scoring of Masson's Trichrome stained paraffin sections of the left lung lobe. BLM induction resulted in a significant 8.3-fold increase in fibrosis score in the left lung lobe in comparison to PBS-induced animals. Oral treatment with dexamethasone or Nintedanib did not result in a significant decrease of group mean histological score in comparison to the PO vehicle group, although dexamethasone treatment showed a decrease of the histological score of 11.7%. Similarly, treatment with Test Article did not result in a significant decrease of the histological score in comparison with the IV vehicle group although the Test Article 10 mg/kg dose showed a decrease of the histological score of 12.3%.

Having noted the high variability in mean Ashcroft Index Scores within groups and also in lung pathology within each individual due to the focal nature of the model, Ashcroft scores were analyzed from all fields scored (13-20 per animal), which increased the number of observations to 84- 182 in BLM-induced groups. Using this comprehensive analysis, treatment with dexamethasone at 0.25 mg/kg and Test Article at 10 mg/kg significantly reduced lung fibrosis in comparison to vehicle- treated controls (see Figure 2).

Summary. The goal of this study was to investigate the therapeutic dose response effects of treatment with Test Article on lung fibrosis in the mouse induced by oropharyngeal administration of BLM. Lung fibrosis was induced by a single oropharyngeal instillation of BLM into the lungs of male C57B1/6J mice. Animals receiving PBS instead of BLM were used as control. The induction of the fibrotic process in the lung was demonstrated by a 19.8-fold increase in BAL cell count and an 8.3- fold increase in fibrosis score in the left lung lobe. An increase of 1.97-fold in total collagen was observed. In addition, also a significant increase in lung wet and dry weights was observed. Taken together, these data support the conclusion that fibrosis was significantly induced in the lungs.

The combination of intravenous injection combined with BLM resulted in a marked loss of animals in all IV injected groups. Although the exact reason for this loss in survival could not be determined, we suspect that the increase in body temperature induced by placing the animals on a heating pad to facilitate IV injections combined with the damage to the lungs by BLM played a role.

Treatment with Test Article resulted in a significant decrease of the total cells in the BAL fluid (i.e., 46%, 38% and 45% for 1, 3 and 10 mg/kg, respectively). Treatment with Test Article starting eight days after the instillation of BLM into the lungs also improved Ashcroft fibrosis score in a dose-related manner (i.e., 4.9%, 8.6%, and 13.1% for 1, 3 and 10 mg/kg, respectively), reaching statistical significance in animals treated with Test Article at 10 mg/kg.

Treatment with dexamethasone (0.25 mg/kg/dose) starting on the day of instillation of BLM into the lungs resulted in a significant decrease in lung wet weight and significant improvement in Ashcroft fibrosis score (11.3%).

Treatment with Nintedanib (60 mg/kg/dose) starting on the day of instillation of BLM into the lungs showed no significant effects in this experiment. The lack of effect is likely a reflection of the high hurdle to ameliorating fibrosis under the conditions of this experiment. Induction in the model is known to vary between and within experimental replicates and Nintedanib has been shown to improve fibrosis at a similar dose level and dosing paradigm in the literature (see Wollin et al., The Journal of Pharmacology and Experimental Therapeutics 349:209-220, 2014).

Conclusions. Overall, Test Article showed strong disease-ameliorating activity in the BLM- induced lung inflammation and fibrosis model when dosed therapeutically, that is beginning 8 days after BLM instillation. Cell counts obtained in the BAL fluid are thought to reflect the degree of immune cell infiltration of the lungs. Test Article significantly decreased these cells, strongly suggesting that it decreased immune cell infiltration of the lungs. In contrast, neither the anti-fibrotic, Nintedanib, nor the immunosuppressant, dexamethasone, had any effect, despite their prophylactic administration. Therapeutic intervention with Test Article also improved (decreased) histological fibrosis 21 days after BLM instillation, demonstrating the anti-fibrotic potential of the molecule. Dexamethasone had a similar effect on this endpoint, but Nintedanib was without effect. In this study the Test Article outperformed Nintedanib, which is marketed for the treatment of idiopathic pulmonary fibrosis. These results suggest that HRS Polypeptides such as the Test Article display significant therapeutic utility for the treatment of lung inflammation and fibrosis in a broad range of ILDs.

Example 2

Evaluation of HRS polypeptide-Fc fusion Proteins for the Treatment of Bleomycin-Induced

Interstitial Lung Disease

Studies were performed to determine if an exemplary HRS-Fc fusion protein (HRS ^FC1 ) reduces respiratory impairment and/or lung fibrosis in a rat model of bleomycin-induced the lung fibrosis. The effects of the HRS-Fc fusion protein on the inflammatory and fibrotic processes were determined by measuring histological fibrosis and inflammation scores, and lung collagen content.

Protocol and Methods. Lung inflammation and fibrosis were induced in male Sprague Dawley (Crl:CD(SD)) rats by oropharyngeal administration of bleomycin (BLM) at a dose level of approximately 1 mg/kg in 100 μΐ for up to 7 consecutive days. PBS or BLM was administered under isoflurane anesthesia. The first day of BLM administration was defined as Day 1. Rats were sacrificed at day 22 using a terminal bleed after carbon dioxide administration. Animals were treated as shown in Table E3 below.

Table E3

Day 1 2 3 4 5 66 77 8 9 10 11 12 13 14 15 16 17 18 19 20 21 control saline IT φ ψ PS PS

Vehicle PO 4· V 4· V 4· V V v v i- v v psv v V V V V PSV V V

Nintedanib or Pirfenidone Ψ PS - Ψ

Vehicle IV ! ⁵ SV

HRS ^FC1 0.1 mg/kg IV

HRS ^FC1 0.3 mg/kg IV PS4-

PS *

HRS 3 mg/kg IV i ^: S - »S4

PS 4·

n = 8/group male SD rats; Daily BWs and survival

4- Bleomycin to lung

V - vehicle

4- - dose administration

P - whole body plethsmography (prior to dose administration)

5 - serum (post-plethsmography, prior to dose adminstration)

X takedown - serum, BALF, lung histology, lung collagen, frozen lung, quadriceps and liver

Due to large body weight loss in animals receiving BLM, oropharyngeal administrations were skipped on Days 4 and 5. Animals receiving PBS on Days 1, 2, 3, 6 and 7 served as non-diseased controls. Animals receiving BLM oropharangeally on Days 1, 2, 3, 6 and 7 received either no therapeutic treatment, oral vehicle (water), oral Nintedanib (60 mg/kg in water), intravenous (IV) vehicle (20 mM His, 150 mM NaCl, pH 6.9), or Test Article (HRS ^FC1 ; Lot# H-Fcv3-N4-921) diluted in the IV vehicle at 0.1, 0.3, 1, or 3 mg/kg. Daily oral treatments commenced on Day 1, while weekly IV treatments commenced on Day 9 (all dose levels) or Day 2 (1 mg/kg).

In-life, body weights were measured and clinical observations were conducted daily. On Day 8 and Day 15, animals were removed from their home cages and placed individually into whole body plethysmography chambers. After a 20-minute acclimatization phase, respiratory parameters were recorded for 10 minutes.

At termination, rats were sacrificed using carbon dioxide and exsanguination via heart puncture one day after the last oral dosing (non-diseased controls, nintedanib and oral vehicle groups) or 6 days after the last IV administration of Test Article.

The five lung lobes were collected and weighed. The right lung lobes were inflated and fixed in 10% neutral buffered formalin and embedded in paraffin for histological analysis. An approximately 50 mg piece of the left lung lobe was snap frozen and stored at -80 °C for potential RNA analysis. The remainder of the lobe was also snap frozen until analyzed for collagen content.

Sections for histology were stained using picrosirius red and were scored in a blinded fashion (1 score for each of the 4 lobes) by a veterinary pathologist using a modified Ashcroft score (Hubner et al., 2008). Hydroxyproline content was determined in the left lung lobe using a mass spectrometry method.

All statistical analyses were performed using GraphPad Prism (GraphPad Software, San Diego, CA). Groups were compared using the one way ANOVA test, followed, when appropriate by a post-hoc Dunnett t-test versus the BLM + appropriate vehicle group.

Results - Survival. Several animals required euthanasia for humane reasons due to severe body weight loss induced by BLM induction. There were 2 animals in groups 3 and 8, respectively. Three of the euthanasias were conducted prior to Test Article administration. The remaining euthanasia was conducted in group 8 (Test Article at 3 mg/kg) on Day 13. Since this animal had lost 11% of its body weight prior to the commencement of treatment, the euthanasia was not attributed to an adverse effect of the Test Article.

Results - Body Weights. Based on data from animals which survived to scheduled necropsy, administration of BLM in the lungs resulted in a decrease of body weight of the rats versus non- diseased controls throughout the experiment which is a characteristic of this model. None of the groups treated with Nintedanib or Test Article had body weight curves that significantly differed from their respective controls.

Results - Respiratory Measurements. Respiratory measurements were conducted on Day 8 and showed a clear impairment induced by BLM. The results are shown in Table E4 below.

way , groups y t test. p< . , p< . , p< . . Significant decreases in expiration and inspiration time were observed, whereas peak expiratory flow, peak inspiratory flow, respiratory minute volume (RMV), and respiratory rate were significantly increased. Tidal volumes were similar between BLM- and non-induced animals on Day 8.

Respiratory measurements conducted on Day 15 (Table E4) show continued impairment in BLM-induced vehicle treated groups. Treatment with Nintedanib did not significantly impact BLM- induced alterations in respiratory parameters. Test Article, in contrast, significantly reversed all respiratory measurements impaired by BLM induction (expiration and inspiration time were increased towards non-diseased controls, and peak expiratory flow, peak inspiratory flow, RMV, and respiratory rate were decreased towards non-diseased controls). Interestingly, tidal volume, which was not significantly elevated by BLM exposure, was decreased by Test Article treatment.

Graphical representation of RMV is shown in Figure 3. RMV is the volume of gas inspired or expired per minute. As shown in Figure 3, RMV is significantly elevated by BLM induction (compare Vehicle groups to group which received Saline IT), likely reflecting physiological compensation for impaired gas exchange between the alveolar space and the blood. Gas exchange is likely poor due to ongoing tissue edema secondary to the high level of immune cell infiltration and release of cytokines described in the model by others. Nintedanib has no impact on RMV (or any other respiratory measures). Test Article beginning on Day 9 or on Day 2., in contrast, significantly decreased this endpoint at all dose levels, In fact, RMV is normalized (i.e., not different than oropharyngeal Saline) by Test Article treatment.

Results - Lung mass and collagen content. Lung mass (wet weight) obtained at necropsy on Day 22 and collagen content were significantly elevated by BLM induction on Days 1, 2, 3, 4, 6 and 7. None of the treatment groups significantly changed lung wet weight or collagen content.

Results - Histological Scores. Slides were read by a veterinary pathologist and each of the 4 lobes evaluated was assigned 4 scores [Ashcroft for fibrosis (scores range from 0 to 8),

perivascular/peribronchiolar inflammatory cell infiltrate (scores range from 0 to 5),

interstitial/alveolar inflammatory cell infiltrate (scores range from 0 to 5), type II pneumocyte hyperplasia (scores range from 0 to 5)].

Ashcroft scores assigned to PBS-induced animals were uniformly scored 0, while BLM- induced vehicle treated animals had scores ranging from 2 to 6 on the 8-point scale, confirming significant fibrosis had been induced. Oral treatment with Nintedanib decreased group mean histological scores 5% in comparison to the PO vehicle group, which did not meet statistical criteria. Similarly, treatment with Test Article did not result in a significant decrease of the histological score in comparison with the IV vehicle group although the group receiving Test Article at 1 mg/kg on Days 2, 9 and 16 showed a decrease of the Ashcroft score of 15.3%.

Having noted the high variability in mean Ashcroft Index Scores within groups and also in lung pathology within each individual due to the focal nature of the model, we analyzed Ashcroft scores from all fields scored (4 per animal), which increased the number of observations to 24-32 in BLM-induced groups. Using this comprehensive analysis, treatment with Test Article at 1 mg/kg on Days 2, 9 and 16 significantly reduced lung fibrosis in comparison to vehicle-treated controls (see Figure 4).

Perivascular/peribronchiolar inflammatory cell infiltrate and type II pneumoycte hyperplasia was absent in all of the sections from Saline-induced non-diseased controls.

Perivascular/peribronchiolar inflammatory cell infiltrate was absent in all but 2 animals receiving Vehicle PO and 1 animal receiving Vehicle IV, in which minimal to mild (score = 1-2) infiltrate was observed. No statistical evaluation was done on this endpoint since BLM had little effect. Similarly, minimal (score = 1) type II pneumocyte hyperplasia was observed in some sections from BLM- induced animals, but was not affected by treatment.

Interstitial/alveolar inflammatory cell infiltrate was absent (score = 0) in all sections from Saline-induced lungs. In animals induced with BLM, minimal to moderate (score = 1-3) interstitial inflammatory cell infiltrate was observed as shown in Figure 5. Oral treatment with Nintedanib had no effect on interstitial inflammatory cell infiltrate whereas treatment with Test Article at 1 mg/kg on Days 2, 9, and 16 significantly reduced interstitial inflammatory cell infiltrate in comparison to vehicle-treated controls.

Summary. The overall objective of this study was to determine whether the Test Article reduces the extent of respiratory impairment and/or lung fibrosis in a rat model of bleomycin-induced the lung fibrosis. Lung fibrosis was induced by five oropharyngeal instillations of BLM into the lungs of male Sprague Dawley rats during the first 7 days of the experiment. Animals receiving Saline instead of BLM were used as control. The induction of inflammation and fibrotic processes in the lung was demonstrated by histological observation of minimal to moderate interstitial/alveolar inflammatory cell infiltrate and Ashcroft fibrosis scores of 2 to 6 in BLM-induced animals.

Respiratory measures conducted at Day 8 and Day 15 showed significant changes induced by BLM, persisting in animals that received Vehicles, supporting the hypothesis that gas exchange was impaired, likely by inflammation-associated lung edema. Taken together, these data support the conclusion that inflammation and fibrosis were significantly induced in the lungs, with pathological changes present by at least Day 8.

Treatment with Test Article resulted in a striking resolution of respiratory measures conducted on Day 15. Whereas Vehicle- treated animals had stable values between Day 8 and Day 15, Test Article treatment elicited significant changes in all measures towards (and in many cases matching) the measures obtained in Saline-induced non-diseased controls. The activity of Test Article was observed across dose levels (0.1-3 mg/kg). Treatment of Test Article also resulted in a significant decrease (improvement) in Ashcroft score and interstitial inflammation measured on Day 22 in rats that received 1 mg/kg on Days 2, 9 and 16. Treatment with Nintedanib (50 mg/kg/dose) starting on Day 9 showed no significant effects in this experiment. The lack of effect is likely a reflection of the high hurdle to ameliorating fibrosis under the conditions of this experiment. Induction in the model is known to vary between and within experimental replicates and Nintedanib has been shown to improve fibrosis at a similar dose level in the literature (see Chaudhary et al., The European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology. 29:976-985, 2007).

Conclusions. Overall, Test Article showed strong disease-ameliorating activity in the BLM- induced lung inflammation and fibrosis model when dosed therapeutically, that is beginning 2 or 9 days after starting BLM instillation. Respiratory measures were the most sensitive endpoint in this study, showing significant effects at all doses and paradigms tested (0.1-3 mg/kg administered weekly). In contrast, but not surprisingly given the likely reliance of respiratory measures on immune- driven interstitial edema in this model, the anti-fibrotic, Nintedanib had no effect.

Therapeutic intervention with Test Article also improved (decreased) histological fibrosis and interstitial immune cell infiltration measured on Day 22, further substantiating the immune modulatory potential and demonstrating the anti-fibrotic potential of the molecule. The Test Article outperformed Nintedanib, which had no significant effects in the study, but is marketed for the treatment of idiopathic pulmonary fibrosis. These results suggest that HRS Polypeptides such as the Test Article display significant therapeutic utility for the treatment of lung inflammation and fibrosis in a broad range of ILDs.

Example 3

Evaluation of HRS polypeptide-Fc fusion Protein in Combination with Nintedanib or Pirfenidone for the Treatment of Bleomycin-induced Interstitial Lung Disease

Studies were performed to determine if an exemplary HRS-Fc fusion protein (HRS ^FC1 ) in combination with Nintedanib or Pirfenidone, which are marketed for treatment of IPF, reduces respiratory impairment and/or lung fibrosis in a rat model of bleomycin-induced the lung fibrosis. The effects of the HRS-Fc fusion protein on the inflammatory and fibrotic processes were determined by measuring respiratory measures, histological fibrosis and inflammation scores, and lung collagen content. The circulating levels of HRS ^FC1 were also determined.

Protocol and Methods. In the same laboratory using methods identical to those described in Example 2, lung inflammation and fibrosis were induced in male Sprague Dawley (Crl:CD(SD)) rats by oropharyngeal administration of bleomycin (BLM) at a dose level of approximately 1 mg/kg in 100 μΐ for up to 7 consecutive days. PBS or BLM was administered under isoflurane anesthesia. Excess rats were induced with BLM and used to replace any animals with excessive body weight loss. The first day of BLM administration was defined as Day 1. Rats were sacrificed at Day 17 or 22 using a terminal bleed after carbon dioxide administration. Animals were treated as shown in Table E5-A and E5-B below. Table E5-A

n = 12/group male SD rats in two cohorts, A (n = 4, term Day 16) and B (n = 8, term Day 30); 3X/week BWs and daily survival 4· Bleomycin to lung

V - vehicle— super convenient if we can use the same vehicle for nintedanib and pirfenidone

N - nintedanib, 60 mg/kg/day PO

P - pirfenidone, 100 mg/kg/dose X 3 doses/day PO

ATYR - HRS ^FC1 3 mg/kg IV

WBP - whole body plethsmography

S - serum

takedown - serum, lung wet and dry weight, lung histology (1 lobe), tissues in RNALater: lung, quadriceps takedown - serum, lung histology, lung collagen content, tissues in RNALater: lung, quadriceps

Due to large body weight loss in animals receiving BLM, oropharyngeal administrations were skipped on Days 4 and 7. Animals receiving PBS on Days 1, 2, 3, 5 and 6 served as non-diseased controls (group 1). Animals receiving BLM oropharangeally on Days 1, 2, 3, 5 and 6 received either oral vehicle (0.5% CMC and 0.5% Tween-80) 3 times daily plus IV vehicle (20mM His, 125mM NaCl, lOmM Methionine, 3% Sucrose, 0.02% PS20, pH 6.9) once weekly (group 2), oral Nintedanib (60 mg/kg) once daily at midday plus oral vehicle twice daily in the morning and evening plus IV vehicle once weekly (group 3); oral Pirfenidone (70 mg/kg per dose) three times daily plus IV vehicle once weekly (group 4), oral vehicle three times daily plus Test Article (HRS ^FC1 ; 3 mg/kg; Lot# H- Fcv3-N4-1005) once weekly (group 5), oral Nintedanib once daily at midday plus oral vehicle twice daily in the morning and evening plus IV Test Article once weekly (group 6), or oral Pirfenidone three times daily plus IV Test Article once weekly (group 4). All treatments commenced on Day 9.

In-life, body weights were measured and clinical observations were conducted daily. On Days 8, 15 and 21, animals were removed from their home cages and placed individually into whole body plethysmography chambers. After a 20-minute acclimatization phase, respiratory parameters were recorded for 10 minutes. Pre-study and on Days 10 and Day 17, whole blood was obtained from the tail vein and processed to serum for measurement of HRS ^FC1 .

At termination on either Day 17 (n = 4) or Day 22 (n = 8), rats were sacrificed using carbon dioxide and exsanguination via heart puncture one day after the last oral dosing (non-diseased controls, nintedanib and oral vehicle groups) or 6 days after the last IV administration of Test Article.

The five lung lobes were collected and weighed. On Day 17, the right accessory lobe was retained and desiccated to obtain dry weight. The remaining right lung lobes were inflated and fixed in 10% neutral buffered formalin and embedded in paraffin for histological analysis. An

approximately 50 mg piece of the left lung lobe was snap frozen and stored at -80 °C for potential RNA analysis. The remainder of the lobe was also snap frozen until analyzed for collagen content.

Sections for histology were stained using picrosirius red and were scored in a blinded fashion (1 score for each of the 3-4 lobes) by a veterinary pathologist using a modified Ashcroft score (Hubner et al., 2008). Hydroxyproline content was determined in the left lung lobe using a mass spectrometry method.

Serum samples from animals treated with HRS ^FC1 were used to determine levels of the protein in systemic circulation using standard sandwich ELIS A techniques with antibodies that capture the HRS moiety and detect the Fc portion of the fusion protein.

All statistical analyses were performed using GraphPad Prism (GraphPad Software, San Diego, CA). Groups were compared using the one-way ANOVA test (histology, lung weights, collagen content) or two-way repeated measures ANOVA test (pharmacokinetics), followed, when appropriate by a post-hoc Dunnett test.

Results - Survival. All animals that initiated treatments on Day 9 survived to scheduled necropsy.

Results - Body Weights. Administration of BLM in the lungs resulted in a decrease of body weight of the rats versus non-diseased controls throughout the experiment which is a characteristic of this model. None of the groups treated with Nintedanib, Pirfenidone or Test Article alone or in combination had body weight curves that significantly differed from their respective controls. Results - Respiratory Measurements. Respiratory measurements were conducted on Day 8 and showed a clear impairment induced by BLM (Table E6).

Table E6: Respiratory Measurements

DAY 8

BLM

Disease Vehicle IV Test Article (3 mg/kg)

Control VehPO Ninte. PO Pirf.PO VehPO Ninte. PO Pirf.PO

0.263 0.158 0.151 0.153 0.146 0.148 0.148

Expiratory

Time (0.016) (0.013) (0.012) (0.014) (0.009) (0.011) (0.011)

0.180 0.113 0.115 0.114 0.117 0.117 0.113

Inspiratory

Time (0.014) (0.006) (0.007) (0.006) (0.008) (0.007) (0.006)

Peak 10.4 19.1 18.4 19.5 19.7 22.6 18.0

Expiratory

Flow (1.2) (2.0) (1.7) (2.0) (1.6) (2.4) (1.7)

Peak 13.5 18.3 17.2 17.5 18.0 19.8 16.9

Inspiratory

Flow (1.7) (1.8) (1.5) (1.5) (1.6) (2.0) (1.7)

Respiratory 218.1 313.1 304.5 314.8 310.9 341.0 304.5

Minute

Volume (28.1) (32.9) (28.7) (29.6) (26.8) (35.8) (28.9)

170.2 244.6 249.0 247.6 247.8 251.3 250.9

Respiratory

Rate

(16.1) (15.7) (16.0) (16.3) (14.1) (16.1) (15.5)

1.32 1.23 1.19 1.24 1.22 1.31 1.19

Tidal

Volume (0.059) (0.060) (0.066) (0.051) (0.049) (0.071) (0.065)

DAY 15

BLM

Disease Vehicle IV Test Article (3 mg/kg)

Control VehPO Ninte. PO Pirf.PO VehPO Ninte. PO Pirf.PO

0.288 0.201 0.205 0.193 0.179 0.192 0.191

Expiratory

Time (0.014) (0.019) (0.016) (0.014) (0.016) (0.018) (0.010)

0.183 0.138 0.122 0.135 0.126 0.129 0.128

Inspiratory

Time (0.012) (0.009) (0.007) (0.009) (0.009) (0.011) (0.006)

Peak 9.5 14.2 16.5 15.0 17.5 18.1 14.2

Expiratory

Flow (0.5) (1.8) (1.3) (1.5) (2.1) (2.3) (1.2)

Peak 13.2 15.2 19.0 15.6 18.1 19.1 15.8

Inspiratory

Flow (1.0) (1.7) (1.6) (1.7) (2.0) (1.9) (1.2)

Respiratory 203.4 262.7 309.5 260.2 309.2 307.6 260.1

Minute

Volume (14.7) (31.9) (27.5) (26.8) (39.9) (36.9) (20.4)

153.6 203.1 223.4 206.2 229.1 220.4 205.6

Respiratory

Rate

(10.5) (19.3) (14.6) (14.5) (19.3) (16.5) (12.0)

1.41 1.27 1.35 1.23 1.30 1.33 1.27

Tidal

Volume (0.03) (0.05) (0.06) (0.06) (0.06) (0.07) (0.05)

DAY 21

BLM

Disease Vehicle IV Test Article (3 mg/kg)

Control VehPO Ninte. PO Pirf.PO VehPO Ninte. PO Pirf.PO 0.247 0.199 0.231 0.234 0.21 1 0.210 0.223

Expiratory

Time (0.020) (0.027) (0.018) (0.020) (0.019) (0.019) (0.018)

0.145 0.120 0.127 0.148 0.137 0.160 0.135

Inspiratory

Time (0.01 1) (0.010) (0.006) (0.01 1) (0.01 1) (0.017) (0.010)

Peak 12.5 15.4 13.9 13.0 14.6 14.9 12.5

Expiratory

Flow (1 .2) (1 .6) (1 .3) (1 .1) (2.1) (2.1) (0.9)

Peak 17.3 20.3 18.1 15.8 17.1 15.9 16.5

Inspiratory

Flow (1 .4) (1 .6) (1 .2) (1 .5) (2.3) (2.5) (1 .2)

Respiratory 274.5 334.5 286.0 259.6 282.4 264.1 263.7

Minute

Volume (26.4) (36.2) (22.0) (25.2) (44.8) (46.1) (22.2)

199.0 231 .7 203.7 186.5 199.6 192.9 199.3

Respiratory

Rate (18.8) (20.3) (12.7) (17.9) (20.8) (23.0) (13.5)

1 .50 1 .48 1 .44 1 .43 1 .39 1 .33 1 .37

Tidal

Volume (0.040) (0.052) (0.071) (0.048) (0.065) (0.087) (0.068)

Data shown are mean ^SEM (in brackets). Significant differences from animals receiving vehicle both PO and IV is shown by bold font (2 -way ANOVA followed by Dunnett's post-hoc)

On Day 8, prior to any treatments, significant decreases in expiration and inspiration time were observed, whereas peak expiratory flow and respiratory rate were significantly increased. Tidal volumes, peak inspiratory flow, and respiratory minute volume (RMV) were similar between BLM- and non-induced animals on Day 8 in this experiment.

Respiratory measurements conducted on Day 15 and Day 21 (Table E6) showed declined impairment in BLM-induced vehicle treated groups, suggesting rapid spontaneous resolution, which has been reported in bleomycin models. Treatment with Nintedanib, Pirfenidone or Test Article did not significantly impact BLM-induced alterations in respiratory parameters, in this study - likely because of this rapid spontaneous resolution of disease.

Results - Lung mass and collagen content. Lung mass (wet weight) obtained at necropsy on Day 17 or Day 22 and collagen content measured on Day 22 were significantly elevated by BLM induction on Days 1, 2, 3, 5 and 6. None of the treatment groups significantly changed lung wet weight or collagen content (data not shown).

Results - Histological Scores. Slides were read by a veterinary pathologist and each of the 3 lobes evaluated was assigned 4 score types [Ashcroft for fibrosis (scores range from 0 to 8), perivascular/peribronchiolar inflammatory cell infiltrate (scores range from 0 to 5),

interstitial/alveolar inflammatory cell infiltrate (scores range from 0 to 5), type II pneumocyte hyperplasia (scores range from 0 to 5)].

Ashcroft scores assigned to PBS-induced animals were uniformly scored 0, while BLM- induced vehicle treated animals had scores ranging from 1 to 5 on the 8-point scale, confirming significant fibrosis had been induced, albeit more moderate that observed in example 2. Intervention with Nintedanib, Pirfenidone or Test Article, alone or in combination had no significant effect on interstitial inflammatory cell infiltrate in comparison to controls receiving PO plus IV vehicles (data not shown).

Interstitial/alveolar inflammatory cell infiltrate was absent (score = 0) in all sections from Saline-induced lungs. In animals induced with BLM, minimal to moderate (score = 1-3) interstitial inflammatory cell infiltrate was observed. Intervention with Nintedanib, Pirfenidone or Test Article, alone or in combination had no significant effect on interstitial inflammatory cell infiltrate in comparison to controls receiving PO plus IV vehicles (data not shown).

Results: PK. Serum levels of HRS ^FC1 were detectable on Days 10, 17 and 22 in animals receiving Test Article HRS ^FC1 on Days 9 and 16. As expected, levels of HRS ^FC1 decreased between Days 17 and 22, demonstrating clearance of the Test Article over time. HRS ^FC1 was also measurable in animals that were also receiving Nintedanib or Pirfenidone. Surprisingly, however, the levels of HRS ^FC1 , measured ~24 hours post dose were significantly increased by the concomitant treatment with the small molecules marketed for the treatment of IPF in this rodent model of lung inflammation and fibrosis induced by oropharyngeal administration of BLM (Figure 6).

Summary. The overall objective of this study was to determine whether the Test Article reduces the extent of respiratory impairment and/or lung fibrosis in a rat model of bleomycin-induced the lung fibrosis. Lung fibrosis was induced by five oropharyngeal instillations of BLM into the lungs of male Sprague Dawley rats during the first 7 days of the experiment. Animals receiving Saline instead of BLM were used as control. The induction of inflammation and fibrotic processes in the lung was demonstrated by histological observation of minimal to moderate interstitial/alveolar inflammatory cell infiltrate and Ashcroft fibrosis scores of 1 to 5 in BLM-induced animals.

Respiratory measures conducted at Day 8 showed significant changes induced by BLM, although these declined in animals that received Vehicles. Taken together, these data support the conclusion that inflammation was transiently induced in the lungs, with pathological changes present by at least Day 8 and that moderate inflammation and fibrosis persisted until Day22.

Intervention beginning on Day 9 with Nintedanib, Pirfenidone or Test Article was without significant effect in this experiment, in contrast to the experiment summarized in Example 2, which was likely related to the rapid spontaneous resolution and transient nature of the response in this study.

Conclusions. Overall, none of the interventions (Nintedanib, Pirfenidone or Test Article) tested were effective in ameliorating disease measures in this experiment, alone or in combination. These results are potentially secondary to the milder phenotype elicited in this experimental cohort, since both Nintedanib and Pirfenidone have been reported as efficacious in this model.

Although the Test Article and small molecules did not affect model efficacy endpoints, the HRS ^FC1 data clearly demonstrate that both Nintedanib and Pirfenidone interact with pharmacokinetic characteristics of the Test Article, which is an engineered protein. To confirm the observation that Nintedanib and Pirfenidone altered the PK of HRS , we evaluated a second study conducted in the same laboratory using methods identical to those described in Example 3. Surprisingly, concomitant administration of both Nintedanib and Pirfenidone increased the amount of HRS ^FC1 measured in serum 24 hours after IV injection (data not shown) confirming the initial observation. In both cases, the effect is more marked after the 2 ^nd injection. This novel and unexpected observation of altered PK of HRS ^FC1 , an engineered HRS polypeptide, in the presence of two small molecule therapies for IPF with distinct mechanisms of action, suggests that these molecules interact either directly or on shared pathways in the context of inflammatory and fibrotic diseases.