RELATED APPLICATIONS

This application claims priority to US provisional application 62/525,471 filed on

June 27, 2017, the contents of which are incorporated by reference in their entirety.

FIELD OF THE INVENTION

The invention relates to pharmaceutical compositions comprising a conjugate of protoporphyrin IX and a recombinant protein, wherein the conjugate comprises high levels of protoporphyrin IX. The invention also relates to methods of producing such pharmaceutical compositions and to methods of using such pharmaceutical compositions in the treatment of diseases, such as cancer. BACKGROUND OF THE INVENTION

Photodynamic therapy (PDT) is a therapeutic strategy and treatment procedure that uses a photosensitizer to produce reactive oxygen species in order to kill malignant cells or other disease cells. PDT can be used to treat cancers (including bladder, cervical, esophageal, gastric, lung and skin cancers) and also non-malignant diseases (including acne, macular degeneration and psoriasis). In PDT, upon exposure to irradiating light (at a wavelength appropriate for the photosensitizers), the photosensitizers are excited from the ground state (So) to the first singlet state (Si) and then the longer lived triplet state (Tj) via an intersystem crossing system. After excitation, the photosensitizers return to the ground state (So) from the triplet state (Tj), resulting in an emission of light (fluorescence) or transferring of energy to another particle or system (radiation-less transition). In the latter transition process (radiation-less transition), many reactive oxygen species are produced (ROS; including singlet oxygen/ ¹ (¾, hydrogen peroxide/H2(¾, and superoxide radicals/02 ), which can kill tumor cells by directly damaging them as well as by destroying the tumor vasculature. Among these ROS, ¹ C>2 is the active predominant cytotoxic agent in PDT. PDT is also an ideal therapeutic procedure for cancer treatment because it is clinically approved and minimally invasive. Protoporphyrin IX (PpIX; 7,12-diethenyl 3,8, 13,17-tetramethyl-2111 ,2311- porphine 2, 18-dipropanoic acid) has been utilized as a photosensitizer in both PDT and photodynamic diagnosis (PDD). PpIX is a metal-free porphyrin that is the direct precursor of heme. The biological heme biosynthesis pathway is a conservative process among prokaryotes (e.g. , bacteria) and eukaryotes (including humans). Ferrochelatase is the terminal enzyme in the heme biosynthesis and is responsible for the enzymatic insertion of Fe ²⁺ into PpIX.

There are issues with both direct and indirect application of PpIX in PDT. For example, direct topical application of PpIX can induce increased levels of PpIX in tumor tissues. However, PpIX has some intrinsic problems, particularly its high hydrophobicity and low solubility in aqueous solutions. Its low solubility in water can cause

accumulation in the skin, resulting in prolonged photosensitivity up to several weeks after PDT treatment. For systematic application of PpIX, its poor localization or targeting of cancer cells also limits its direct application for PDT.

An alternative approach to direct topical application of PpIX is indirect application of PpIX by mediation with 5-aminolevulinic (5-ALA), the precursor of PpIX biosynthesis, a treatment called "ALA-PDT." In this procedure, 5-ALA is administered and enters the heme biosynthesis pathway in the cancer cells and then stimulates the PpIX synthesis that causes PpIX accumulation. Upon excitation with light irradiation, the cancer cells are eradicated. However, the accumulation of PpIX in cancer cells may vary in different cancer types that depend on the cellular concentration of ALA, the activity of enzymes involved and some other cellular factors. Thus, the therapeutic application of ALA-PDT is limited with respect to some cancer types because it is difficult to achieve high levels of PpIX accumulation. During the past years, many efforts have been made to improve the ALA-PDT treatment procedure, by modifying or conjugating ALA to carriers and taken up by target cancer cells. However, these kinds of modification or conjugation are complicated and very expensive which also limit the therapeutic applications.

In view of these limitations, it would be advantageous to develop a method of enabling the selective accumulation or up-take of photosensitizers, such as PpIX, in the target cells relative to the surrounding non-target tissues in order to enhance the effective treatment of the diseased tissues while reducing the collateral side effects. SUMMARY OF THE INVENTION

In one aspect, the invention provides a pharmaceutical composition comprising an effective amount of at least one type of conjugate comprising a porphyrin and a recombinant protein, and a pharmaceutically acceptable excipient. The porphyrin and the recombinant protein may be, for example, PpIX and TF (globin 2αβ2 tetramer). The conjugate of PpIX and TF is referred to herein as TF01.

In another aspect, the invention provides a method of treating cancer. The method includes administering an effective amount of a pharmaceutical composition to an area of a patient in need of treatment. The pharmaceutical composition comprises at least one type of conjugate comprising a porphyrin and a recombinant protein, and a

pharmaceutically acceptable excipient. The method further includes irradiating the area in need of treatment to generate reactive oxygen species after administration of the pharmaceutical composition, thereby treating the cancer.

In another aspect, the invention provides a method of reducing the volume of a tumor in a subject. The method includes administering an effective amount of a pharmaceutical composition to an area of the subject where the tumor is found. The pharmaceutical composition comprises at least one type of conjugate comprising a porphyrin and a recombinant protein, and a pharmaceutically acceptable excipient. The method further includes irradiating the area in which the tumor can be found to generate reactive oxygen species after administration of the pharmaceutical composition, thereby reducing the volume of the tumor in the area of the subject where the tumor is found.

In yet another aspect, the invention provides a method of producing a pharmaceutical composition. The method comprises constructing a vector plasmid comprising a promoter, a recombinant protein, and a selectable marker. The vector plasmid transforms a bacterial strain by inserting the plasmid therein. The bacterial strain is one that endogenously produces PpIX. The method further comprises selecting bacterial clones using an antibiotic corresponding to the selectable marker and culturing the bacterial clones in a fermentation medium. The method also includes the step of purifying conjugates from the fermentation medium, wherein the conjugates comprise PpIX non-covalently associated with the recombinant protein. Optionally, a pharmaceutically acceptable carrier may be added to the purified conjugates, thereby forming the pharmaceutical composition.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 : A schematic diagram of the PpIX bound to the recombinant protein (in this case, TF (human globin 2a and β chain sequences, present as a 2αβ2 tetramer).

FIG. 2: A vector plasmid comprising sequences for a Ptac promoter, the human globin 2a and β chains of TF, and a selection marker, prepared in accordance with one exemplary embodiment of the invention.

FIG. 3: A process flow chart summarizing a PpIX-TF conjugate (referred to herein as TF01) purification process according to one embodiment of the invention.

FIG. 4: The results of the analysis of purified TF (2αβ2 tetramer) and PpIX by LC-MS.

FIG. 5: The results of the ultra-performance liquid chromatography used to measure the amount of hemin and PpIX content in purified conjugate comprising PpIX and recombinant TF.

FIG. 6: Comparison of emission properties of the purified conjugate comprising PpIX-TF conjugate (TF01) solution and the control (PpIX in buffer).

FIG. 7: An illustration of the emission properties of PpIX-TF conjugate (TF01) at different pH ranges.

FIG. 8: FIG. 8(a) shows the cytotoxicity of the PpIX-TF conjugate (TF01) under light excitation by blue light (450 nm laser source), by red light (627 nm laser source), and under dark conditions (control) in the HCT 116 (panel A) and HepG2 (panel B) cancer cell lines. FIG. 8(b) shows IC5 ₀ values determined by curve fitting-statistical software (GraphPad Prism 6) for the HCT116 (panel A) and HepG2 (panel B) cell lines under laser light excitation at 450 nm or 627 nm.

FIG. 9: Fluorescence microscopic image showing cellular uptake of PpIX-TF conjugates (TF01) in a colorectal carcinoma cell line HCT116.

FIG. 10: Measurement of generation of reactive oxygen species by PpIX-TF conjugates (TF01) by comparison with photocatalytic bleaching of p- nitrosodimethylaniline (RNO). FIG. 11: The results of a soft agar colony-formation assay showing the effect of PpIX-TF conjugates (TF01) at a concentration of lOug/ml on the formation of SK-Hep- 1 cancer cell colony by comparison with a medium control.

FIG. 12: shows cellular uptake of PpIX-TF conjugate (TF01) in a liver cancer cell line HepG2 using (a) FITC labeling; (b) Hoechst 33342 staining; (c) Bright Field, microscopy techniques; (d) overlay for (a), (b) and (c); and (e) overlay for (a) and (b).

FIG. 13: shows cellular localization of PpIX-TF conjugate (TF01) in a liver cancer cell line SK-Hep-1 using MitoTracker (a); LysoTracker (b); and ER Tracker (c) labeling techniques.

FIG. 14: Measurement of intracellular reactive oxygen species generation by the

PpIX-TF conjugate (TF01) in HCT116 cancer cells.

FIG. 15: shows in vivo PDT efficacy of PpIX-TF conjugate (TF01) in nude mice by reduction in tumor volume.

FIG. 16: IC5 ₀ values of PpIX-TF conjugate (TF01) under different dosage of 635- nm laser excitation.

DETAILED DESCRIPTION OF THE INVENTION

The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color rawing(s) will be provided by the Office upon request and payment of the necessary fee.

In one aspect, the invention provides a pharmaceutical composition comprising a conjugate that comprises a porphyrin (e.g. , PpIX) and a protein. The pharmaceutical compositions of the invention are useful for a variety of different purposes, including the treatment of cancer in connection with photodynamic therapy. The invention recognizes that the conjugate comprising protein bound to the porphyrin acts as a carrier for the porphyrin, preventing the self-aggregation of the porphyrin and the improvement of the porphyrin's properties including water solubility, dispensability, fluorescence, and generation of reactive oxygen species. This invention also recognizes that conjugates comprising porphyrin and a protein, as described herein, will undergo enhanced uptake by target cells, including cancer cells. Accordingly, the invention recognizes that the pharmaceutical compositions of the invention will exert enhanced photo-damage on targeted cancer cells when applied in connection with photodynamic therapy. In addition, the invention recognizes that the PpIX-protein conjugates described herein minimize undesirable side effects commonly associated with application of unconjugated PpIX in photodynamic therapy.

In preferred embodiments of the invention, the pharmaceutical compositions contain a conjugate comprising PpIX and a protein, as described herein. It is to be understood, however, the invention also contemplates the use of other porphyrins, instead of or in addition to PpIX, that are capable of generating therapeutic reactive oxygen species upon irradiation with electromagnetic radiation of the appropriate wavelength. Non-limiting examples of such porphyrins include modified protoporphyrin IX, meta tetrahydroxyphenyl chlorin (mTHPC), benzoporphyrin derivative (BPD) and metal binding protoporphyrins, such as zinc protoporphyrin. In general, the conjugates of the invention may be formed by combining a porphyrin, such as PpIX, with a protein capable of binding with the porphyrin, such as, for example any protein known to bind non- covalently with heme. Without wishing to be limited by theory, it is believed that the porphyrin (e.g., PpIX) is non-covalently associated with the protein in the conjugate, as evidenced by the observation that PpIX can be isolated from conjugates of the invention by extraction with HCl-acetone. In this context, the term "non-covalently associated" refers to binding that does not result from the formation of one or more covalent bonds between the porphyrin and the protein, but rather from intermolecular forces such as hydrogen bonding, London forces, dipole-dipole interactions, and the like. A schematic illustration of such a non-covalently associated conjugate is provided in FIG. 1. As shown in FIG. 1, the PpIX-TF conjugate (referred to herein as TFOl) comprises a protein with one di-alpha chain and two beta chains, with which PpIX molecules (here two for the purposes of illustration) are non-covalently associated. In certain embodiments, the porphyrin and the protein are obtained separately and physically combined to form the conjugate. For example, the protein may be recovered from natural sources or produced recombinantly and then combined with the porphyrin. In other embodiments, the porphyrin and protein are produced together. For example, when the porphyrin is PpIX, the invention contemplates producing the protein recombinantly in bacteria that naturally produce PpIX. Bacteria that naturally produce PpIX have been reported, for example, by Fyrestam et al. See Fyrestam, J., Bjurshammar, N., Paulsson, E. et al. Anal. Bioanal. Chem. (2015) 407:7013. The invention recognizes that PpIX naturally binds via non- covalent association to proteins when the proteins (e.g., globular proteins) are expressed in certain types of bacteria. In this way, the PpIX -protein conjugate can be produced cost- effectively and efficiently by using suitable fermentation and purification techniques, as described herein.

The proteins that are used in the conjugates of the invention are not particularly limited and can be any protein that does not cause any adverse biological effect on a patient and that is capable of non-covalently associating with PpIX or other porphyrins to form a therapeutically useful conjugate for photodynamic therapy. In certain preferred embodiments, the protein is a globular protein. Non-limiting examples of suitable proteins include TF (Globin 2αβ2 tetramer) (SEQ ID NO: 1 and 2), myoglobin (SEQ ID NO: 3), cytochrome P450 (SEQ ID NO: 4), cytochrome C (SEQ ID NO: 5), nitric oxide (NO) synthase (isoform 1, SEQ ID NO: 6, isoform 2, SEQ ID NO: 7, isoform 3, SEQ ID: NO. 8), Rev-erba (SEQ ID NO: 9) and Rev-erb (SEQ ID NO: 10). As noted previously, the protein may be produced recombinantly in bacterial strains that naturally produce PpIX, so that the PpIX-protein conjugate can be recovered efficiently. Some examples of useful bacterial strains include, without limitation, the Jml09(DE3), clear coli, and BL21(DE3) strains of E. coli. In one particularly useful embodiment, the recombinant protein is TF and a PpIX-TF conjugate (TF01) is formed as described herein. Generally, the native human sequences may be used in the methods of the invention described herein. In some embodiments, fragments or subunits of di-alpha chains or beta chains or gamma chains of globins may be used as the recombinant protein. In a preferred embodiment, the recombinant protein is TF and contains one di-alpha chain (-32 kDa) and two beta chains (-16 kDa for each chain).

Suitable conjugates of the invention include those in which the porphyrin-protein molar ratio is the range of about 0.05 to about 4.00, or about 0.10 to about 2.00, or about 0.5 to about 1.0, with the understanding that the end points of each of these ranges may be combined to form additional sub-ranges that are within the scope of the invention. In some embodiments, the porphyrin and the protein are prepared separately and then combined together to form the therapeutic conjugates of the invention. For example, when the porphyrin is PpIX and the protein is TF, the PpIX may be isolated from bacterial sources and the TF may be isolated from serum-derived sources or

recombinantly and then combined together to form a PpIX-TF conjugate (TF01) with a suitable molar ratio of PpIX to TF. In certain preferred embodiments, however, the porphyrin-protein conjugates are obtained from a bacterial strain that is capable of producing both the desired porphyrin and protein. For example, a PpIX-TF conjugate (TF01) may be produced by recombinantly expressing TF in bacterial strains that also produce PpIX. In such cases, substantially amounts of the PpIX-TF conjugates (TF01) may be recovered efficiently by suitable fermentation and recovery steps as described herein. When PpIX-TF conjugates are formed in this manner, useful PpIX-TF molar ratios are typically in the range of about 0.05 to about 4.

The invention also provides pharmaceutical compositions comprising porphyrin- protein conjugates of the invention. In certain preferred embodiments, the

pharmaceutical compositions comprise only one type of conjugate (e.g. , PpIX-TF), but the invention also contemplates adding two or more different types of conjugates when producing pharmaceutical compositions. Preferably, the conjugates are present in the pharmaceutical compositions in an effective amount. As used herein, the term "effective amount" refers to an amount that is implicitly safe, but sufficient to provide a therapeutic benefit to a patient in connection with photodynamic therapy. In addition to the one or more types of conjugates, the pharmaceutical compositions may also include a pharmaceutically acceptable carrier. Non-limiting examples of pharmaceutically acceptable carriers include solvents (e.g. , buffered saline), bulking agents, viscosity modifying agents, surfactants, dispersing agents, isotonic agents, coatings, release control agents, and the like.

The pharmaceutical compositions of the invention may be administered to a patient in a variety of different ways including intravenous, intratumoral, intraperitoneal, or even subcutaneous injection, to name just a few. In certain embodiments, the pharmaceutical compositions are applied topically to the area in need of treatment prior to irradiation by light of an appropriate wavelength to generate reactive oxygen species. In such embodiments, it is sometimes useful to include viscosity modifying agents in the pharmaceutical composition to help the pharmaceutical composition remain localized after topical application. Alternatively, the pharmaceutical compositions may be directly injected into an area in need of treatment. The light that interacts with the pharmaceutical compositions of the invention to generate the reactive oxygen species may be supplied directly to the application area by irradiating the skin with an external light source or by delivering the light via a surgically inserted fiber optic cable, depending on the depth of administration and the wavelength of light used. In certain preferred embodiments, the light has a wavelength in the range of 300 nm to 700 nm. For example, when the porphyrin-protein conjugate is PpIX-TF (TF01), it is advantageous to use light with a wavelength of 630 nm. Examples of suitable light sources include lasers (e.g. , those with an output wavelength in within the visible / UV spectrum) as well as non-coherent light sources. Non-limiting examples of useful light intensities include those in the range of about 20 J/cm ² to about 200 J/cm ² .

Using the pharmaceutical compositions and methods disclosed herein, one can treat a variety of different cancers and other diseases. Non-limiting examples of cancers that may be treated include bladder, cervical, esophageal, gastric, lung and skin cancers. Typically, treatment involves administering the pharmaceutical compositions of the invention to the area in need of treatment (e.g. , a tumor) and irradiating with an appropriate wavelength of light to generate reactive oxygen species that have a cytotoxic effect on the cancer cells. The pharmaceutical compositions of the invention may also be used for treating any other disease (e.g. , acne) that can be treated using photodynamic therapy. Furthermore, because the pharmaceutical compositions of the invention are readily taken up by cancer cells, they can be used in connection with a fluorescence diagnosis to map the size and shape of a tumor.

The invention also provides a method of producing porphyrin-protein conjugates recombinantly in high yield. The method comprises inserting sequences for the recombinant protein, a promoter, and a selectable marker into a cloning vector to generate a plasmid vector. Non-limiting examples of suitable cloning vectors include, for example, pUC-19, pBR322, pET, pUC, and so on. Furthermore, non-limiting examples of suitable promoters include Ptac, T7, T71ac, pL, Lac, and so on. For example, when the desired recombinant protein is TF, a useful plasmid vector may be formed by inserting the sequences for a Ptac promoter, the human globin 2a and β chains of TF, and beta-lactamase into a pUC19 to generate a ρΙΙ09-2αβ plasmid, as illustrated in FIG. 2. The vector plasmid is transformed into the bacterial strain. In certain embodiments of the invention, the plasmid is transformed into a bacterial strain and grown overnight at a temperature in the range of 15-40°C, preferably about 37°C. Appropriate bacterial clones are identified using the selectable marker. For example, when the selectable marker causes the expression of beta-lactamase, bacterial clones can be identified using a beta- lactam antibiotic, such as ampicillin.

After appropriate bacterial clones are selected, the bacterial clones are mass produced. In one embodiment, mass production of the bacterial clones is achieved using a two-step fermentation process. In the first step, a seed culture medium is inoculated with the selected bacterial clones. In general, the seed culture medium is not particularly limited and can be any culture medium typically used for production of recombinant proteins by fermentation. For example, in certain embodiments, the culture medium comprises yeast extract and a particularly useful seed culture medium comprises yeast extract (6%) and NaCl (1%). In the second step, after a suitable fermentation period, the seed culture may be added to another culture medium. This second culture medium also is not particularly limited and may comprise ingredients that are typically used for large scale production of recombinant proteins by fermentation. In one exemplary

embodiment, the culture medium comprises yeast extract 1 %, tryptone 1.6%, K2HPO4 14.7 mM, KH ₂ P0 ₄ 37 mM, NaCl 15.1 mM, (NH ₄ ) ₂ S0 ₄ 15.14 mM, L-proline 1.998 mM, and glycerol 2%. In addition, while the protein sources in the culture media for producing the recombinant proteins may be derived from animal sources, the invention also contemplates embodiments in which all of the protein sources are "animal-free" (i.e. , not derived from any animal products). Non-limiting examples of such protein sources include hydrolyzed soy (e.g. , soy peptone), yeast extract, and corn steep liquor. Such embodiments are useful for avoiding issues relating to prion contamination, which can lead to Creutzfeld-Jakob disease. In certain embodiments, fermentation during the second step of the two-step fermentation process continues until the Οϋβοο reaches a value in the range of 10-50, preferably 20-45, and even more preferably 30-40. In certain preferred embodiments, the second step of the two-step fermentation process may contain two or more phases. For example, as described herein, the second step of the two-step fermentation process may include an aerobic phase, followed by an induction phase in which isopropyl- -d-thiogalactoside (IPTG) is added to induce protein expression.

After the fermentation step is completed, the bacterial clones are harvested. In certain preferred embodiments, the harvesting comprises centrifuging the fermentation medium to form a cell pellet. To lyse the cells in the cell pellet, the cell pellet may be resuspended in a suitable lysis buffer and then homogenized. Preferably, the soluble fraction of the lysate is passed through a filter membrane before subsequent purification steps. Subsequent purification steps may include, for example, a series of

chromatographic purifications to recover the porphyrin-protein conjugate. For example, when the desired porphyrin-protein conjugate is PpIX-TF , the PpIX-TF conjugate (TFOl) may be recovered by passing the lysate through a first DEAE anion exchange column (pH 8.5) , a second DEAE anion exchange column (pH 7.5), and a CM cation exchange column (pH 6.5) as shown in FIG. 3 and described in the Examples provided herein. EXAMPLES EXAMPLE 1

Construction of 2a Globin and β Globin Expression Plasmid

SEQ ID NO: 1 and SEQ ID NO: 2 corresponding to human globin 2a and β chain sequences (i.e. , the TF, 2αβ2 tetramer) were inserted into a pUC19 vector to generate a ρΙΙ09-2αβ plasmid along with the sequence for an expression protein (Ptac promoter). See FIG. 2. The plasmid was transformed into the Jml09(DE3), Clear coli and

BL21(DE3) E. coli strains and grown overnight at 37°C. Bacterial clones were identified using antibiotic selection (ampicillin, 100 g/ml). EXAMPLE 2

Co-expression of 2a Globin and β Globin by fermentation

Kcoli (Jml09(DE3), Clear coli and BL21 (DE3)) clones transformed with pUC19- 2αβ were inoculated into 200 ml of seed culture medium (yeast extract 6% and NaCl 1%) and cultivated at 37°C with shaking at 250 rpm for overnight. The fermentation was performed in 10-L bioreactor (Sartorius C plus). The flask of seed culture was inoculated into 6.5 L of medium (yeast extract 1 %, tryptone 1.6 %, K ₂ HP0 ₄ 14.7 mM, KH ₂ P0 ₄ 37 mM, NaCl 15.1 mM, (NH4) ₂ S0 ₄ 15.14 mM, L-proline 1.998 mM, glycerol 2%) in a 10-L bioreactor with a final QDeoo at 0.05 and cultivated at 32°C and pH 7.0 with airflow of 6 L/min and initial stir rate at 400 rpm, in which dissolved oxygen was maintained above 30%.

The fed-batch process of recombinant protein production in 10-L bioreactor was divided into two phases. Phase I was aerobic batch cultivation at 32°C for 7 h. Phase II was an induction process at 20°C for 16 b using isopropyl-P-d-ttaogaiactoside (IPTG) at a final concentration of 0.4 uM with ODeoo of bacteria at 4.0 to induce the expression of recombinant protein. A fed-batch cultivation at 20°C for 16 h in which 80 g/L glycerol was fed continuously to maintain the required specific growth rate of bacteria with airflow of 8 L/min and stir rate at 600-800 rpm, in which dissolved oxygen was maintained above 20%. The bacteria at final ODeoo of 30-40 were harvested by centrifugation and stored in -80°C for future use.

EXAMPLE 3

Purification of PpIX-TF conjugates (TF01)

Cell lysis and clarification of crude sample

As illustrated by the process flow diagram in FIG. 3, the cell pellet from fermentation was resuspended in a solubilization buffer (20 mM Tris-HCl, pH 8.5) in 1 g cell to 10 ml buffer ratio. DNase was added to the suspension to a final concentration of 2 μg/ml and incubated at room temperature for 30 min. The suspension was then sonicated by Sonics Vibra-cells™ sonicator at 70% amplitude for at least 10 min. For clarification, the cell lysate was centrifuged in a pre-chilled rotor at 35,000 g for at least an hour. The supernatant (soluble fraction) of the lysate was adjusted to pH 8.5 and conductivity to lower than 2.7 mS/cm. The supernatant was passed through a 0.45 μηι PES filter membrane before loading onto chromatographic column.

Chromatographic purification

To remove impurities from the conjugate, the soluble fraction which contained solubilized PpIX-TF conjugates (referred to herein as TF01)was passed through a series of chromatographic columns to isolate the PpIX-TF conjugates from various impurities.

First, the supernatant was loaded onto DEAE anion-exchange column which was pre-equilibrated with running buffer 1 (20 mM Tris-HCl, pH 8.5). After sufficient column wash with running buffer 1, the PpIX-TF conjugates (TF01) were eluted by several sodium chloride step elutions (5 mM NaCl, 1.5 CV; 10 mM NaCl, 3 CV; 20 mM NaCl, 1.5 CV; 40 mM NaCl, 2.5 CV; 100 mM NaCl, 1 CV; 1 M NaCl, 2 CV). The 40 mM NaCl elution fractions which contained the PpIX-TF conjugates (TF01) were collected. Second, the recovered fraction was concentrated to 10 mg/ml and buffer- exchanged to running buffer 2 (25 mM potassium phosphate buffer, pH 7). The sample was then loaded onto a DEAE anion-exchange column which was pre-equilibrated with the running buffer 2. The PpIX-TF conjugates (TFOl) appeared in the column flow- through during sample loading and column wash. After sufficient column wash with running buffer 2, the bound proteins were eluted by 1 M NaCl for 4 CV.

Third, the recovered fraction was adjusted to pH 6.5 and conductivity lower than 3.2 mS/cm. The PpIX-TF sample was then loaded onto CM cation exchange column which was pre-equilibrated with the running buffer 3. After sufficient column wash with running buffer 3, PpIX-TF conjugates (TFOl) were eluted by several sodium chloride step elutions (0.25 M NaCl, 4 CV; 1 M NaCl, 4CV). The 0.25 M NaCl elution fractions which contained PpIX-TF (TFOl) were collected.

Finally, the purified PpIX-TF (TFOl) sample was concentrated to 30 mg/ml and then it was passed through a PALL Mustang™ E membrane for removal of endotoxin.

The protein sample was sealed in a glass vial under deoxygenated environment. The glass vial was vacuum-sealed in a light-proof plastic bag and stored at 4°C.

Table 1 shows the results of five exemplary batches of PpIX-TF conjugates

(TFOl), made in accordance with the method provided in Examples 1-3. Isolated PpIX- TF conjugates (TFOl) were suspended in 20 mM Tris buffer. PpIX and TF levels were measured using UPLC.

Table 1: Different Batches of PpIX-TF conjugates (TFOl)

PpIX level

PpIX-TF conjugate sample Molar Ratio of PpIX to TF

Cone. (mM)

protein

Batch 1 0.054 0.690

Batch 2 0.088 0.911

Batch 3 0.062 0.619

Batch 4 0.049 0.633

Batch 5 0.177 0.705 EXAMPLE 4

Characterization of PpIX-TF conjugates (TF01)

Liquid chromatography-mass spectrometry (LC-MS)

The purified protein was suspended in 100 μΐ and analyzed by LC-MS. FIG. 4 depicts the results of liquid chromatography-mass spectrometry which was used to measure the alpha chains, beta chains and PpIX content in the purified conjugate comprising PpIX and recombinant TF (2αβ2 tetramer) which was suspended in 100 μΐ after chromatographic purification step. FIG. 4(A) depicts the alpha chain and beta chain peaks, and FIG. 4(B) depicts the peaks associated with the PpIX portion of the purified recombinant protein, TF.

Ultra Performance Liquid Chromatography (UPLC)

The protein concentrations of the samples were measured by Bradford protein assay (Bradford Dye Reagent and Bovine Serum Albumin). Samples were diluted to protein concentration between 5 - 15 mg ml. The purified protein was mixed with 400 μΐ acidic acetone (sample to organic solvent equals 1 :8). After vigorous stirring, the mixture was separated by centrifugation. Acetonitrile (ACN, 400 ul, sample to organic solvent equals 1:8) was added into the solution, followed by centrifugation at 14,000 rpm for 5 mill. Samples were then applied to ultra- performance liquid chromatography (UPLC) analysis (Acquity I I -class UPLC system; Waters, Milford, MA, USA).

PpIX was separated by Waters Acquity UPLC ^® BEH CI S 1.7 μπι, 2.1 x 50mm Column at 0.50 ml/min at 25 °C for 15 min (Eluent: A: FLO; B: ACN [0.1% TFA]) Gradient: 30% B to 50% B in 6 min, 50% B to 80% B in 12 min, 80% B to 30% B in 13 min, 30% B in 15 min. (Detection wavelength at 400 m). Commercially obtained PpIX (Sigma- Aldrich, St. Louis, MO, USA) was used as the standard. The peak of PpIX was identified and the peak area in the calibration standards and samples was recorded. The peak area of calibration standards was plotted against the PpIX concentration of working standards. Thus, the relative amount of PpIX was obtained using the calibration curve and associated equation.

FIG. 5 depicts the results of the ultra-performance liquid chromatography used to measure the amount of hemin and PpIX content in purified conjugate comprising PpIX and recombinant TF (2αβ2 tetramer). FIG. 5 shows that the conjugate is comprised of over 70% PpIX compared to 26% hemin, indicating that there is a greater concentration of PpIX than hemin in the purified conjugate comprising PpIX and recombinant TF.

UV-vis absorption and fluorescence spectroscopy

A BMG fluostar spectrofluorophotometer was used to record fluorescent emissions of PpIX or PpIX-TF conjugate (referred to herein as TF01) suspended in aqueous buffer. A UV-vis spectrophotometer was used to record the absorbance spectra of PpIX and TF.

FIG. 6 depicts the emission properties of the purified conjugate comprising PpIX and recombinant TF and the control (PpIX in buffer). FIG. 6 demonstrates that the emission properties of the recombinant protein TF bound to PpIX (top curve in FIG. 6, Batch 1, 5 mg/ml; corresponding to approximately 53 μΜ PpIX content) in Tris-HCl buffer (20 mM, pH 8.5; red) are greater than the emission properties of the control PpIX (bottom curve in FIG. 6, 60 μΜ PpIX) in Tris-HCl buffer (20 mM, pH 8.5, with 5% DMSO; blue).

FIG. 7 depicts the pH dependence of the emission intensity of TF in 50mM Tris- HCl or potassium phosphate buffer when monitored at a _max = 625 nm. Many studies suggest that the pH of solid tumors is around 7.0-7.2 whereas extracellular pH may be more acidic. FIG. 7 indicates that the PpIX-TF conjugate (TF01) of the invention is suitable for application in PDT therapy due to its wide pH range.

Solubility and stability

Improvement in solubility and dispersibility of the PpIX-TF conjugate was compared to PpIX in aqueous Tris-HCl buffer, 20 mM, pH 8.5 (FIG. 6).

Cell phototoxicity assay- To determine cell viability under dark conditions, cells (1 x 10 ⁴ cells/well) were seeded onto 96-weli plates and incubated for 24 h at 37 C. After cell stabilization, the culture medium was replaced with 100 μΐ of culture medium containing free PpIX or PpIX-TF conjugate (referred to herein as TF01), followed by incubation for 24 h. The ceils were then washed twice with serum-free medium and ceil viability was evaluated by MTT assay after 48 h. To determine the in vitro phototoxicity after laser irradiation, cells (1 x 10 ^" ceils/well) were seeded onto 96-well plates and incubated for 24 h at 37°C. The cells were then treated with free PpIX or a PplX-TF conjugate (TFOl). After 24 h incubation, the cells were washed twice with serum-free medium and irradiated at a light intensity of 150 mW/cm' using 450 nm or 627 nm LED light source for 30 min or 10 mW/cm using a 635-nm laser source for 5-30 min (3 il cm ^" to 18 J/ cm ^' ). The ceil viability of irradiated cells was evaluated by MTT assay after 24 h of incubation.

FIG. 8(a) and 8(b) depict the cytotoxic effect of PpIX-TF conjugate (referred to herein as TFOl) as demonstrated by the MTT assay. FIG. 8(a) shows the cytotoxicity of PpIX-TF conjugate (TFOl) under light excitation (450 nm (blue) or 627 nm (red) light source) and under dark (control) is compared in two different cancer cell lines (A) HCT 116 and (B) HepG2. FIG. 8(b) shows the IC5 ₀ values under light excitation (450 nm (blue) or 627 nm (red) light source), determined by curve fitting-statistical software (GraphPad Prism 6) in two different cancer cell lines (A) HCT 116 and (B) HepG2. For each cancer cell line, measurements were performed in dark and in the presence of 450 nm blue laser light or 627 nm red light. The data show that the survival rates of the cells from both cancer lines markedly decrease upon irradiation with the 450 nm blue laser light and 627 nm red laser light respectively when the PpIX-TF conjugate (TFOl) concentration is greater than about 25 g/ml.

FIG. 16 shows the cytotoxicity of PpIX-TF conjugate (TFOl) under different dosages of 635-nm laser excitation. The data show that the IC5 ₀ values of PpIX-TF conjugate (TFOl) were dosage dependent. When laser dosage were increased, the corresponding IC5 ₀ values of PpIX-TF conjugate (TFOl) were decreased.

Soft agar colony-formation assay

To estimate the anchorage-independent growth ability of cells treated by PpIX-TF conjugate (referred to herein as TFOl), 3xl0 ⁴ cells of SK-Hep-1 cell were seeded in 35mm dish and incubated for 24 h at 37°C. The cells were then treated with a PpIX-TF conjugate (TFOl) or medium control. After 24 h incubation, the cells were washed twice with serum-free medium and irradiated at a light intensity of 10 mW/cm using a 635-nm laser source for 5 min. Then, 5000 cells were suspended in 0.3% noble agar and cells were allowed to grow for another 2 weeks. Colonies were stained with 0.02% Giemsa Stain Solution, and documented by scanning.

FIG. 11 depicts the anti-colony-formation effect of PpIX-TF conjugate (TFOl) as demonstrated by the soft agar colony-formation assay. Treated cells by 10 ug/ml of PpIX-TF conjugate (TFOl) and irradiated at a light intensity 3 J/cm using a 635-nm laser source will significantly inhibit the cancer cell soft agar colony formation.

Ceil uptake and imaging

Cancer cells were used to detect and compare cellular uptake of PpIX and PpIX- TF conjugate (referred to herein as TFOl) in vitro. The cells were cultured in medium supplemented with 10% fetal bovine serum at 37°C and 5% C(¾. After incubating cells with PpIX-TF conjugate (TFOl), images were viewed by confocal microscopy.

FIG. 9 depicts the fluorescence microscopic analysis of cellular uptake of recombinant TF in a colorectal carcinoma cell line HCT1 16. The intense fluorescent signals of the purified ΡρΓΧ-TF conjugates (TFOl) in FIG. 9 reveals that the PpIX-TF conjugates (TFOl ) are widely distributed in the cytoplasm of the colorectal carcinoma cells (represented by arrows in the micrograph).

FIG. 12 shows the cellular uptake of PpIX-TF conjugate (TFOl) in a liver cancer cell line HepG2. ΡρΙΧ-TF conjugates (TFOl) were labeled with FITC, stained with Hoechst 33342 or illuminated using Bright field microscopy; the results of such labeling, staining and microscopy techniques are depicted in figures (a)-(e):(a) FTTC-TF01 ; (b)

Hoechst 33342; (c) Phase contrast, Bright field; (d) overlay the images of (a), (b) & (c); and (e) overlay the images of (a) & (b).

FIG. 13 shows cellular localization of PpIX-TF conjugate (TFOl) in a liver cancer cell line SK-Hep-I. The Mitotracker, lysotracker and ER-cracker (Thermo Fisher

Scientific) were used to label mitochondria, lysosome and endoplasmic reticulum (ER) respectively. Two measures of correlation, the Pearson correlation coefficient (PCC) and the Mander's overlap coefficient (MOC) were calculated as depicted in the table below.

The results demonstrated the co-localization of PpIX-TF conjugates (TFOl ) in different organelles of the cell: mitochondria, lysosome and endoplasmic reticulum. Pearson correlation coefficient (PCC) Mander's overlap coefficient (MOC)

Mitochondria 0.4922 0.4684

Lysosome 0.4974 0.4866

Endoplasmic reticulum 0.6076 0.6908

Reactive oxygen species (ROS) generation

To determine singlet oxygen generation, singlet oxygen detection method was applied to a 3 ml aqueous solution containing the singlet oxygen generator, 50 M of RNO and 8 mM of imidazole. The detection for absorption intensity of RNO near 440 nm was monitored to reflect the production of ROS by a UV-vis spectrophotometer after time intervals of laser exposure.

FIG. 10 depicts the ROS generation by the conjugate comprising PpIX and PpIX-

TF conjugate (referred to herein as TFOl) against the positive control of methylene blue. FIG. 10 depicts that the quantum yield of ROS of the conjugate of PpIX and recombinant TF was almost double that of methylene blue. These results indicate that the conjugate would be effective as a photosensitizer in PDT or PDD.

To examine intracellular ROS generation by PpIX and PpIX-TF conjugate

(TFOl), HCT116 cells were seeded in 24-well, glass-bottomed plate and incubated with cellrox reagent (Thermo Fisher Scientific). After 30 min, the cells were washed three times by PBS and treated with PpIX or PpIX-TF conjugate (TFOl). After 24 h of incubation, the ceils were washed twice with PBS and then exposed to a 10 mW/cm ⁱ using a 635-nm laser source for 30 min. After irradiation, the fluorescence intensity of cells was measured by a microplate reader with excitation/emission at 485/520 nm. The values are expressed as fluorescence intensity ratio to control.

FIG. 14 depicts the intracellular ROS generation by the PpIX-TF conjugate in the HCT1 16 cancer cells. IOOUM H2O2 _, as a positive control, increase the intracellular ROS generation. PpIX-TF conjugates (TFOl) (50 urn/ml (batch 2) contains

approximately .71uM PpIX) greatly enhance the intracellular ROS generation, which is much higher than the intracellular ROS generation of the control PpIX (at a concentration of .75 uM). Sodium azide (NaN ₃ , a scavengers of ROS), the specific quencher of (¾, can partially abolish this effect.

EXAMPLE 5

in vivo PDT efficacy

To test the in vivo PDT efficacy of PpIX-TF conjugate, nude mice bearing human hepatoma SK-HEP 1 xenograft were intravenously treated with PBS, 200 mg/kg or 400 mg/kg PpIX-TF conjugate (TF01) respectively (n=3-4 per group), followed by the laser application (at a light intensity 230 mW/cm 2 , 260 J/cm 2 , using a 635-nm laser source). Visible tumors were measured using two orthogonal measurements L and W

(perpendicular to L), the volumes were calculated using the formula V = LW 12 and recorded. FIG. 15 shows the representative digital photos of tumors before and after treatment. The figure shows the absolute tumor volumes (upper panel) and their percentage changes (lower panel). The treatment with 400 mg/kg TF01 followed by the laser application results in tumor regression.

While the above described embodiments of the invention are described in terms of preferred ranges, these preferences are by no means meant to limit the invention. As would be readily understood by one skilled in the art, the preferred amounts and ingredients used in the pharmaceutical compositions depend on the method of administration, the conjugate used, and the like. Likewise, actual release rates and release duration depend on a variety of factors in addition to the above, such as the disease state being treated, the age and condition of the patient, the route of admini stration, as well as other factors which would be readily apparent to those skilled in the art. All of the foregoing U.S. Patents and other publications are expressly incorporated by reference herein in each of their entireties.

From the foregoing description, one of ordinary skill in the art can easily ascertain the essential characteristics of the instant invention, and without departing from the spirit and scope thereof, can make various changes and/or modifications of the invention to adapt it to various usages and conditions. As such, these changes and/or modifications are properly, equitably and intended to be, within the full range of equivalence of the following claims. SEQ ID NO: 1: Protein sequence of 2a Chain of TF

MLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSA QVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCL LVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYRGMLSPADKTNVKAAWGK VGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNA VAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHA SLDKFLASVSTVLTSKYR

SEQ ID NO: 2: Protein sequence of β Chain of TF

MHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDA VMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLL GNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

SEQ ID NO: 3: Myoglobin

MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSED

EMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFIS

ECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

SEQ ID NO: 4: cytochrome P450

MINMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVP EFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGM RGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETD VDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLE EDFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLK SYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAV YPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDI TNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQ DCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKG VATNWLRAKEPAGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIG FIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFS REQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAE LGAMEHAQAVDYIKKLMTKGRYSLDVWS

SEQ ID NO: 5: cytochrome C

MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAAN KNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

SEQ ID NO: 6: nitric oxide synthase (Isoform 1)

MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQS GLIQAGDIILA VNGRPLVDLS YDS ALEVLRGI ASETH VVLILRGPEGFTTHLETTFT GDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDG QEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSR GVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPV VLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLE TGCTEYICMGSIMHPSQHARRPED VRTKGQLFPLAKEFIDQY YSSIKRFGSKAHM ERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDC TTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYK QPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVL EVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVR DYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVD HHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFE YQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATIL YATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNG DPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRD NFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELC GQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFV AEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQP GDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDEL RLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWK WGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVS YRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGT GIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFR ELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVL

KAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAF

IEESKKDTDEVFSS SEQ ID NO: 7: nitric oxide synthase (Isoform 2)

MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQ NESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGIL TCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLAR VEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTA REMFEHICRHVRYSTNNGNIRS AITVFPQRSDGKHDFRVWNAQLIRYAGYQMPD GSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEV AMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVR DFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMD HHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFY YYQVE AWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTIL FATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNG DCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGA SQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTW DPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSC EDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYW VSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYS KWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAV VTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGP GTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQK GVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMA RDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRV AVQPSSLEMSAL

SEQ ID NO: 8: nitric oxide synthase (Isoform 3)

MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPS SPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQG RPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRE SELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATN RGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCI QHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRW YALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDL DTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKAR GGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITR KKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAF DPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSS PRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRA YPHFC AFAR A VDTRLEELGGERLLQLGQGDELCGQEE AFRGWAQ A AFQ A ACET FCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATI RSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVE DPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLR LLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQ LPLLQPRYYSVSS APSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPG DPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMT LVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELA AEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLR DQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP

SEQ ID NO: 9: Rev-erba

MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPT GSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSP SKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRC LKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSA MNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPS PEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQG CPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLC PTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMS FTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVM FLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRS GMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKL LSFRVDAQ

SEQ ID NO: 10: Rev-erb

MEVNAGGVIAYISSSSSASSPASCHSEGSENSFQSSSSSVPSSPNSSNSDTNGNPKN GDLANIEGILKNDRIDCSMKTSKSSAPGMTKSHSGVTKFSGMVLLCKVCGDVAS GFHYGVHACEGCKGFFRRSIQQNIQYKKCLKNENCSIMRMNRNRCQQCRFKKCL SVGMSRDAVRFGRIPKREKQRMLIEMQSAMKTMMNSQFSGHLQNDTLVEHHEQ TALPAQEQLRPKPQLEQENIKSSSPPSSDFAKEEVIGMVTRAHKDTFMYNQEQQE NSAESMQPQRGERIPKNMEQYNLNHDHCGNGLSSHFPCSESQQHLNGQFKGRNI MHYPNGHAICIANGHCMNFSNAYTQRVCDRVPIDGFSQNENKNSYLCNTGGRM HLVCPMSKSPYVDPHKSGHEIWEEFSMSFTPAVKEVVEFAKRIPGFRDLSQHDQV NLLKAGTFEVLMVRFASLFDAKERTVTFLSGKKYSVDDLHSMGAGDLLNSMFEF SEKLNALQLSDEEMSLFTAVVLVSADRSGIENVNSVEALQETLIRALRTLIMKNHP NEASIFTKLLLKLPDLRSLNNMHSEELLAFKVHP