COMPOUNDS AND METHODS FOR TREATING MAMMALIAN GASTROINTESTINAL MICROBIAL INFECTIONS

Title:

COMPOUNDS AND METHODS FOR TREATING MAMMALIAN GASTROINTESTINAL MICROBIAL INFECTIONS

Document Type and Number:

WIPO Patent Application WO/2014/028931

Kind Code:

Abstract:

Disclosed are compounds and pharmaceutically acceptable salts thereof, which are useful as inhibitors of IMPDH. In certain embodiments, a compound selectively inhibits a parasitic IMPDH versus a host IMPDH. Also disclosed are pharmaceutical compositions comprising one or more compounds of the invention. Related methods of treating various parasitic and bacterial infections in mammals are disclosed. Moreover, the compounds may be used alone or in combination with other therapeutic or prophylactic agents, such as anti-virals, anti-inflammatory agents, antimicrobials and immunosuppressants.

Inventors:

HEDSTROM LIZBETH K (US)
CUNY GREGORY D (US)
GORLA SURESH KUMAR (US)
KAVITHA MANDAPATI (US)

Application Number:

PCT/US2013/055585

Publication Date:

February 20, 2014

Filing Date:

August 19, 2013

Export Citation:

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Assignee:

UNIV BRANDEIS (US)
BRIGHAM & WOMENS HOSPITAL (US)

International Classes:

A61K31/502; C07D405/02

Domestic Patent References:

WO2010108187A2

2010-09-23

Other References:

GOLLAPALLI, D ET AL.: 'Structural Determinants Of Inhibitor Selectivity In Prokaryotic IMP Dehydrogenases.' CHEM BIOL. vol. 17, 29 October 2011, pages 1084 - 1091

Attorney, Agent or Firm:

GORDON, Dana M. et al. (155 Seaport Blvd.Boston, MA, US)

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Claims:

We claim:

1. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by

Formula I:

Formula I wherein, independently for each occurrence,

R¹ is alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, haloalkyl, aralkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, formyl, acyl, aracyl, heteroaracyl, carboxyl, alkoxycarbonyl, acyloxy, cyano, -OR', halide, -N(R')₂, azido, nitro, amido, isocyano, phosphonate, phosphinate, silyl, thio, alkylthio, sulfonate, sulfonyl, sulfonamido, or sulfhydryl; wherein R' is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl;

R² is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy;

R³ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; m is 0, 1, or 2; n is 1-4; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein any of the aforementioned alkyl, aryl, heteroaryl, or aralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

2. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula II:

Formula II wherein, independently for each occurrence,

R¹ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, haloalkyl, aralkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, formyl, acyl, aracyl, heteroaracyl, carboxyl, alkoxycarbonyl, acyloxy, cyano, -OR', halide, -N(R')₂, azido, nitro, amido, isocyano, phosphonate, phosphinate, silyl, thio, alkylthio, sulfonate, sulfonyl, sulfonamido, or sulfhydryl; wherein R' is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl;

R² is alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy;

R³ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; m is 0, 1, or 2; n is 1-4; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, heteroaryl, or aralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

3. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula III:

Formula III wherein, independently for each occurrence,

R² is hydrogen, is alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; R³ is alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; m is 0, 1, or 2; n is 1-4; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, heteroaryl, or aralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl formyl, cyano, and isocyano.

4. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula IV:

Formula IV wherein, independently for each occurrence,

R' is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl; R² is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy;

wherein any of the unsubstituted ring positions is optionally substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

5. A compound, or a pharmaceutically acceptable salt or ester thereof, selected from the group of

6. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by

Formula V:

Formula V wherein, independently for each occurrence, m is 0, 1, or 2;

R² is cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, or two R² groups together form a non-aromatic ring containing 3 to 8 carbon atoms; is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein any of the aforementioned alkyl, cycloalkyl, heterocyclyl aryl, heteroaryl, aralkyl, or heteroaralkyl may be substituted with one or more groups independently selected from the group consisting of of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

7. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula VI:

Formula VI wherein, independently for each occurrence, m is 0, 1, or 2;

R² is hydrogen, alkyl, haloalkyl, alkoxylalkyl, alkylamino, alkylthio, cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, or two R² groups together form a non-aromatic ring containing 3 to 8 carbon atoms; is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein any of the aforementioned alkyl, cycloalkyl, heterocyclyl aryl, heteroaryl, aralkyl, or heteroaralkyl may be substituted with one or more groups independently selected from the group consisting of of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

8. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula VII:

Formula VII wherein, independently for each occurrence, m is 0, 1, or 2;

R⁴ is hydrogen, C₂-Cgalkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, aryl, or heteroaryl;

Y is C(R⁶)₂, O, S, NQ, or NOQ wherein R⁶ is independently for each occurrence hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, aryl, or heteroaryl, or two R⁶ together form a ring containing 3 to 8 carbon atoms;

Q is hydrogen, alkyl, aminoalkyl, alkylaminoalkyl, arylaminoalkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, heterocyclylalkyl, aralkyl, heteroaralkyl, aryl, or heteroaryl;

R⁷ and R⁸ are independently hydrogen, alkyl, cycloalkyl, aralkyl, heteroaralkyl, or

R⁷ and R⁸ together form a ring containing 3 to 8 carbon atoms; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, cycloalkyl, heterocyclyl aryl, heteroaryl, aralkyl, or heteroaralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

9. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula VIII:

Formula VIII wherein, independently for each occurrence, m is 0, 1, or 2;

R⁴ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, heteroaralkyl, aryl, or heteroaryl;

Q is hydrogen, alkyl, aminoalkyl, alkylaminoalkyl, arylaminoalkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, heterocyclylalkyl, aralkyl, heteroaralkyl, aryl, or heteroaryl;

R⁷ and R⁸ are independently cycloalkyl, aralkyl, heteroaralkyl, or R⁷ and R⁸ together form a ring containing 3 to 8 carbon atoms;

wherein any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

10. A compound, or a pharmaceutically acceptable salt or ester thereof, selected from the group consisting of

P127 P131

11. A compound or a pharmaceutically acceptable salt or ester thereof, represented by Formula IX:

Formula IX wherein, independently for each occurrence, m is 0, 1, or 2;

R⁹ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, aryl, or heteroaryl;

Y is CH₂, C(R¹⁰)(R^U) O, S, NQ, or NOQ; wherein R¹⁰ and R¹¹ are independently hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aralkyl, heteroaralkyl, aryl, or heteroaryl, or R⁸ and R⁹ together form a ring containing 3 to 8 carbon atoms;

Q is hydrogen, alkyl, aminoalkyl, alkylaminoalkyl, arylaminoalkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, heterocyclylalkyl, aralkyl, heteroaralkyl, aryl, or heteroaryl;

R¹² and R¹³ are independently hydrogen, alkyl, cycloalkyl, aralkyl, heteroaralkyl, or R¹² and R¹³ together form a ring containing 3 to 8 carbon atoms; is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, formyl, cyano, and isocyano; and

X¹ and X² are independently O or NH, with the proviso that X¹ and X² cannot both be NH.

12. A compound, or a pharmaceutically acceptable salt or ester thereof, selected from the group consisting of

13. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula X:

Formula X wherein, independently for each occurrence, X³ is C(R')₂, S, or NR';

wherein R' is hydrogen, halide, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl; m is 0, 1, or 2; n is 0, 1, 2, or 3;

Z is hydrogen, halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, or isocyano; is hydrogen, halogen, alkyl, alkenyl, alkynyl, aralkyl, heteroaralkyl, halogen, amino, amido, acyl, aracyl, heteroaracyl, alkoxy, hydroxyl, carboxyl, alkoxylcarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, isocyano, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; and is hydrogen, alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

14. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula XI:

Formula XI wherein, independently for each occurrence,

X³ is absent, O, C(R')₂, S, or NR'; wherein R' is hydrogen, halide, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl; m is 0, 1, or 2; n is 0, 1, 2, or 3;

R¹⁴ is alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy, or two R¹⁴ groups taken together form a non-aromatic 3-8 membered ring, or two R¹⁴ groups taken together form the side chain of a natural or non-natural D or L amino acid, with the proviso that said side chain is neither glycine nor alanine;

15. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula XII:

Formula XII wherein, independently for each occurrence,

Z is halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, or isocyano; is hydrogen, alkyl, alkenyl, alkynyl, aralkyl, heteroaralkyl, halogen, amino, amido, acyl, aracyl, heteroaracyl, alkoxy, hydroxyl, carboxyl, alkoxylcarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, isocyano, monocyclic or bicyclic cycloalkyl, bicyclic heterocyclyl, bicyclic aryl or bicyclic heteroaryl; and is hydrogen, alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

16. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula XIII:

Formula XIII wherein, independently for each occurrence,

Z is halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, or isocyano; is hydrogen, alkyl, alkenyl, alkynyl, aralkyl, heteroaralkyl, halogen, amino, amido, acyl, aracyl, heteroaracyl, alkoxy, hydroxyl, carboxyl, alkoxylcarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, isocyano, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; and is monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

17. A compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula XIV:

Formula XIV wherein, independently for each occurrence,

Z is hydrogen, halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, or isocyano; is hydrogen, alkyl, alkenyl, alkynyl, aralkyl, heteroaralkyl, halogen, amino, amido, acyl, aracyl, heteroaracyl, alkoxy, hydroxyl, carboxyl, alkoxylcarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, isocyano, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; and is hydrogen, alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, or heteroaryl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

18. A compound or a pharmaceutically acceptable salt or ester thereof, selected from the group consisting of

- 196-

19. A compound or a pharmaceutically acceptable salt or ester thereof, selected from the group consisting of

20. A compound or a pharmaceutically acceptable salt or ester thereof, selected from the group consisting of

- 198-

- 199-

-200-

24. A method of killing or inhibiting the growth of a microbe, comprising the step of contacting said microbe with an effective amount of a compound of any one of claims 1-23.

25. The method of claim 24, wherein said microbe is a protozoan or a bacterium.

26. The method of claim 24, wherein said microbe is a protozoan or a bacterium selected from the group consisting of the genera Toxoplasma, Eimeria, Cryptosporidium, Plasmodium, Babesia, Theileria, Neospora, Sarcocystis, Giardia, Entamoeba, Tritrichomonas, Leishmania, Trypanosoma, Helicobacter, Borrelia, Salmonella, Shigella, Yersinia, Streptococcus, Campylobacter, Arcobacter, Bacteroides, Fusobacterium, Burkholderia, Clostridia, Neisseria, Mycobacterium, and Acinetobacter.

27. The method of claim 25, wherein said microbe is a protozoan; and said protozoan is selected from the group consisting of the genera Cryptosporidium, Entamoeba, Leishmania and Trypanosoma.

28. The method of claim 27, wherein said protozoan is selected from the genus Cryptosporidium .

29. The method of claim 27, wherein said protozoan is Cryptosporidium parvum, Cryptosporidium hominis, or both.

30. The method of claim 25, wherein said microbe is a bacterium; and said bacterium is selected from the group consisting of the genera Acinetobacter, Arcobacter, Bacillus, Bacteroides, Borrelia, Brachyspira, Brucella, Burkholderia, Campylobacter, Clostridia, Coxiella, Enterococcus, Erysipelothrix, Francisella, Fusobacterium, Helicobacter, Lactobacillus, Listeria, Mycobacterium, Neisseria, Pseudomonas, Staphylococcus and Streptococcus.

31. A method of treating or preventing a parasitic infection in a mammal or bird, comprising the step of administering to a mammal or bird in need thereof a therapeutically effective amount of a compound of any one of claims 1-23.

32. A method of treating or preventing a microbial infection in a mammal or bird comprising the step of administering to a mammal or bird in need thereof a therapeutically effective amount of a compound of any one of claims 1-23.

33. The method of claim 32, wherein said microbial infection is caused by a protozoan or bacterium.

34. The method of claim 32, wherein said microbial infection is caused by a protozoan or a bacterium selected from the group consisting of the genera Cryptosporidium, Entamoeba, Leishmania, Trypanosoma, Acinetobacter, Arcobacter, Bacillus, Bacteroides, Borrelia, Brachyspira, Brucella, Burkholderia, Campylobacter, Clostridia, Coxiella, Enterococcus, Erysipelothrix, Francisella, Fusobacterium, Helicobacter, Lactobacillus, Listeria, Mycobacterium, Neisseria, Pseudomonas, Staphylococcus and Streptococcus.

35. The method of claim 33, wherein said microbial infection is caused by a protozoan; and said protozoan is selected from the group consisting of the genera Cryptosporidium, Entamoeba, Leishmania and Trypanosoma.

36. The method of claim 35, wherein said protozoan is selected from the genus Cryptosporidium .

37. The method of claim 35, wherein said microbial infection is caused by Cryptosporidium parvum, Cryptosporidium hominis, or both.

38. The method of claim 33, wherein said microbial infection is caused by a bacterium; and said bacterium is selected from the group consisting of the genera Acinetobacter, Arcobacter, Bacillus, Bacteroides, Borrelia, Brachyspira, Brucella, Burkholderia, Campylobacter, Clostridia, Coxiella, Enterococcus, Erysipelothrix, Francisella, Fusobacterium, Helicobacter, Lactobacillus, Listeria, Mycobacterium, Neisseria, Pseudomonas, Staphylococcus and Streptococcus.

39. The method of any one of claims 31-38, further comprising the step of coadministering to a mammal or bird in need thereof a therapeutically effective amount of an antimicrobial agent.

40. The method of claim 39, wherein said antimicrobial agent is an antibiotic.

41. The method of claim 39, wherein said antimicrobial agent is an antiparasitic.

42. The method of any one of claims 31-41, wherein said infection is in a mammal; and the mammal is a primate, a bovine, an ovine, an equine, a porcine, a rodent, a feline, a mustelid, or a canine.

43. The method of any one of claims 31-41, wherein said infection is in a mammal; and the mammal is a primate.

44. The method of any one of claims 31-41, wherein said infection is in a mammal; and the mammal is a human.

45. The method of any one of claims 31-41, wherein said infection is in a bird; and the bird is a chicken.

Description:

Compounds and Methods for Treating Mammalian

Gastrointestinal Microbial Infections

RELATED APPLICATIONS

This application claims the benefit of priority to United States Provisional Patent Application serial number 61/684,263, filed August 17, 2012, the contents of which are hereby incorporated by reference.

GOVERNMENT SUPPORT

The invention was made with support provided by the National Institutes of Health (Grant Nos. U01 AI-075466 and 1R01AI093459); therefore, the government has certain rights in the invention.

BACKGROUND OF THE INVENTION

Organisms must synthesize nucleotides in order for their cells to divide and replicate. Nucleotide synthesis in mammals may be achieved through one of two pathways: the de novo synthesis pathway; or the salvage pathway. Different cell types use these pathways to differing extents.

Inosine-5 '-monophosphate dehydrogenase (IMPDH; EC 1.1.1.205) is an enzyme involved in the biosynthesis of guanine nucleotides. IMPDH catalyzes the NAD-dependent oxidation of inosine-5 '-monophosphate (IMP) to xanthosine-5 '-monophosphate (XMP) [Jackson R. C. et. al, Nature, 256, pp. 331-333, (1975)]. Regardless of species, the reaction involves the random addition of substrates. A conserved active site Cys residue attacks the C2 position of IMP and hydride is transferred to NAD ⁺, producing NADH and the E-XMP* intermediate. NADH is released and a mobile flap folds into the vacant NADH site, E- XMP* hydrolyzes and XMP is released [W. Wang and L. Hedstrom, Biochemistry 36, pp. 8479-8483 (1997); J. Digits and L. Hedstrom, Biochemistry 38, pp. 2295-2306 (1999); Gan et al, Biochemistry 42, pp 847- 863 (2003)]. The hydrolysis step is at least partially rate- limiting in most IMPDHs examined to date. The enzyme is unusual in that a large conformational change occurs in the middle of a catalytic cycle.

IMPDH is ubiquitous in eukaryotes, bacteria, archaebacteria, and protozoa [Y. Natsumeda & S. F. Carr, Ann. N.Y. Acad., 696, pp. 88-93 (1993)]. Two isoforms of human IMPDH, designated type I and type II, have been identified and sequenced [F. R. Collart and E. Huberman, J. Biol. Chem., 263, pp. 15769-15772, (1988); Y. Natsumeda et al, J. Biol. Chem., 265, pp. 5292-5295, (1990)]. Type I has three isoforms derived from different mR A splicing, with 514, 546 and 595 residues. Type II has 514 amino acids, and shares 84% sequence identity to the 514 isoform of Type I. Both IMPDH type I and type II form active tetramers in solution [Y. Yamada et al, Biochemistry, 27, pp. 2737-2745 (1988)].

Proliferation requires an expansion of the guanine nucleotide pool, so rapidly growing cells depend on IMPDH. Thus human IMPDHs are targets for anticancer chemotherapy [L. Che et al, Curr. Opin. Drug. Discov. Devel, 10, 403-12 92007); E. Olah et al, Adv. Enzyme. ReguL, 46, 176-90 (2006)].

The activity of IMPDH is particularly important in B- and T-lymphocytes. These cells depend on the de novo, rather than salvage pathway to generate sufficient levels of nucleotides necessary to initiate a proliferative response to mitogen or antigen [A. C. Allison et. al, Lancet II, 1179, (1975) and A. C. Allison et al, Ciba Found. Symp., 48, 207, (1977)]. Thus, human IMPDHs are an attractive targets for selectively inhibiting the immune system without also inhibiting the proliferation of other cells.

Inhibitors of IMPDH are also known. U. S. Pat. Nos. 5,380,879 (incorporated by reference) and 5,444,072 (incorporated by reference) and PCT publications WO 94/01105 and WO 94/12184 describe mycophenolic acid (MP A) and some of its derivatives as potent, uncompetitive, reversible inhibitors of human IMPDH type I (K; = 33 nM) and type II (K; = 9 nM). MP A has been demonstrated to block the response of B- and T-cells to mitogen or antigen [A. C. Allison et. al, Ann. N.Y. Acad. Sci., 696, 63, (1993)].

Nucleoside analogs such as tiazofurin, ribavirin and mizoribine also inhibit IMPDH [L. Hedstrom, et. al. Biochemistry, 29, pp. 849-854 (1990); L. Hedstrom et al. Curr. Med. Chem. 1999, 6, 545-561]. These compounds require activation to either the adenine dinucleotide (tiazofurin) or monophosphate derivatives (ribavirin and mizoribine) that inhibit IMPDH. These activation pathways are often absent in the cell of interest. In addition, nucleoside analogs suffer from lack of selectivity and can be further metabolized to produce inhibitors of other enzymes. Therefore, nucleoside analogs are prone to toxic side effects.

Additionally, IMPDH has been shown to play a role in viral replication in some viral cell lines. [S. F. Carr, J. Biol. Chem., 268, pp. 27286-27290 (1993)]. Analogous to lymphocyte and tumor cell lines, the implication is that the de novo, rather than the salvage, pathway is critical in the process of viral replication.

IMPDH is also a promising target for antimicrobial chemotherapy. Microbial infections are now the second leading cause of death worldwide. Many commonly used antibiotics have been rendered ineffective by the upsurge of drug resistance, so there is an urgent need of new antimicrobial therapy. IMPDH2 is an essential gene in Mycobacterium tuberculosis, and deletion of IMPDH attenuates the virulence of many other bacteria. IMPDH inhibitors block the growth of Helicobacter pylori, Staphylococcus aureus, Candida albicans, Pneumocystis carinii, Leishmania donovani, Trypanosoma brucei gambienese, Eimeria tenella, Plasmodium falciparum and Cryptosporidium parvum in culture [L. Hedstrom et al, Curr. Med. Chem., 18, pp. 1909-1918 (2011)]. The prokaryotic IMPDHs share 30-40% sequence identity with the human enzyme, and have significantly different kinetic and functional properties. These observations indicate that specific inhibition of prokaryotic IMPDH can be achieved, and that such inhibitors are likely to have antibiotic acivity. Curiously, Cryptosporidium and several other eukaryotic organisms have prokaryote-like IMPDHs that appear to have been obtained via horizontal gene transfer. Eukaryotic organisms that contain a prokaryotic-like IMPDHs are also likely to be sensitive to prokaryotic IMPDH-specific inhibitors.

Cryptosporidiosis is a severe gastrointestinal disease caused by protozoan parasites of the genus Cryptosporidium. The most common causes of human disease are C. parvum and C. hominis, though disease can also result from C. felis, C. meleagridis, C canis, and C muris infection. Small children, pregnant women, the elderly, and immunocompromised people (e.g., AIDS patients) are at risk of severe, chronic and often fatal infection [Carey, C. M., Lee, H., and Trevors, J. T., Water Res., 38, 818-62 (2004); and Fayer, R., Veterinary Parasitology, 126, 37-56 (2004)]. Cryptosporidium infection is a major cause of diarrhea and malnutrition in the developing world. The Cryptosporidium parasites produce sporelike oocysts that are highly resistant to water chlorination. Several large outbreaks in the U.S. have been linked to drinking and recreational water. Infection rates are extremely high, with disease manifest in 30% of exposed individuals and a 50-70% mortality rate among immuno-compromised individuals. Furthermore, there is a growing and credible concern that these organisms could be deliberately introduced into the water supply in an act of bioterrorism. Effective drugs are urgently needed for the management of cryptosporidiosis in AIDS patients and/or epidemic outbreaks. Cryptosporidum parasites also cause significant disease in domestic livestock, especially calves, lambs, kids, foals, piglets and poultry.

All parasitic protozoa lack purine biosynthetic enzymes and must salvage purines from their hosts, making this pathway an extremely attractive target for developing antiprotozoal drugs. IMPDH is a key enzyme in the purine salvage pathway of C. parvum and general IMPDH inhibitors block parasite proliferation in vitro [N. N. Umejiego et al., J Biol Chem, 279 pp. 40320-40327 (2004); and B. Striepen et al, Proc Natl Acad Sci U S A, 101 pp. 3154-9 (2004)]. The IMPDH protein of C. hominis is identical to that of C. parvum, as is the purine salvage pathway. As discussed above, IMPDH is a validated drug target in immunosuppressive, cancer and viral therapy, so the human enzymes are extremely well studied. Cryptospordium appears to have obtained its IMPDH gene from a proteobacterium. Thus C. parvum IMPDH has very different structure and properties than the human enzymes. IMPDHs from many pathogenic bacteria have similar structures to C. parvum IMPDH [Gollapalli et al, Chem. Biol, 17, 1084-1091 (2010)]. There is a need for selective IMPDH inhibitors that can slow or block parasite and bacterial proliferation. The present invention fulfills this need and has other related advantages.

SUMMARY OF THE INVENTION

One aspect of the present invention relates to compounds and pharmaceutically acceptable salts thereof, which are useful as inhibitors of IMPDH. In certain embodiments, a compound of the invention selectively inhibits a parasitic IMPDH versus a host (e.g., mammalian) IMPDH. Further, the invention provides pharmaceutical compositions comprising one or more compounds of the invention. The invention also relates to methods of treating various parasitic and bacterial infections in mammals and birds. Moreover, the compounds may be used alone or in combination with other therapeutic or prophylactic agents, such as anti-virals, anti-inflammatory agents, antimicrobials and immunosuppressants.

BRIEF DESCRIPTION OF THE FIGURES

Figure 1 depicts the correlation of compounds from Schemes 1-5 with the P Series numbering system.

Figure 2 depicts P Series compounds and their IC ₅₀ values against C. parvum/C. hominis IMPDH. Figure 3 (Table 1) depicts the results of structure activity relationship (SAR) studies of the anilide portion of the P Series compounds against C. parvum/C. hominis IMPDH. Assays as described in the methods section. No significant inhibition of human IMPDH type 2 was observed (IC ₅₀ > 5 μΜ). a. One determination, n.d., not determined

Figure 4 (Table 2) depicts the results of structure activity relationship (SAR) studies of the isopropenyl portion of the P Series compounds with C. parvum/C. hominis IMPDH. a. One determination, n.d., not determined.

Figure 5 (Table 3) depicts the results of structure activity relationship (SAR) studies of the urea portion of the P Series compounds with C. parvum/C. hominis IMPDH. a. One determination, b. n.d., not determined.

Figure 6 (Table 4) depicts the results of structure activity relationship (SAR) studies of oxime and methyloxime compounds in the P Series with C. parvum/C. hominis IMPDH.

Figure 7 (Table 5) depicts the stability of some P Series compounds (e.g., compounds depicted in Figures 1-6) in mouse liver microsomes and plasma, n.d., not determined.

Figure 8 (Table 6) depicts antiparasitic activity of selected P Series compounds (e.g., compounds depicted in Figures 1-6). Assays were run as described in Sharling, L., et al. PLoS Negl Trop Dis 2010, 4 (8), e794. Unless otherwise stated all values are the average of three (3) independent determinations.

Figure 9 depicts HPLC retention times and purities for various P Series compounds (e.g., compounds depicted in Figures 1-6). Instrument: Agilent 1100; Column: Zorbax® SB-C8 column, 30 x 4.6 mm, 3.5 μιη; Injection volume: 5 μί; Sample concentration: 1-2 mg/mL in 100 % acetonitrile; λ: 254 nm; Elution solvent: 5 % acetonitrile and 95 % water (both solvents contain 0.1 % trifiuoroacetic acid) with a total run time of 2.5 min.; Elution rate: 3.0 mL/min.

Figure 10 depicts D Series compounds and their corresponding IC ₅₀ values against C. parvum/C. hominis IMPDH.

Figure 11 (Table 7) depicts a SAR of anilide and nathylide phthalazinone inhibitors in the D Series with C. parvum/C. hominis IMPDH. All values are an average of three independent determinations unless otherwise noted (* two determinations). ND, not determined. Figure 12 depicts a SAR of heterotricyclic anilide pthalazinone inhibitors with C. parvum/C. hominis IMPDH. All values are an average of three independent determinations unless otherwise stated. ND, not determined.

Figure 13 (Table 8) depicts antiparasitic activity of selected D Series compounds. Values are the average and standard deviations of three independent determinations unless otherwise stated (* denotes average and range of two determinations), a. T. gondii RH (Toxo/WT) should be resistant to Q?IMPDH inhibitors while T. gondii/ CplMPOU (Toxo/Q?IMPDH) should be sensitive, b. Selectivity = EC ₅₀ (Toxo/WT)/EC ₅₀ (Toxo/Q?IMPDH).

Figure 14 depicts Q Series compounds and their corresponding IC ₅₀ values against C. parvum/C. hominis IMPDH.

Figure 15 (Table 9) depicts a SAR of the phenyl ring in Q Series compounds with C. parvum/C. hominis IMPDH. IC ₅₀, inhibition of C. parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T. gondii strain expressing endogenous T. gondii IMPDH that should be resistant to C. parvum IMPDH inhibitors; CpIMPDH, a T. gondii strain that relies on the expression of C. parvum IMPDH that should be sensitive to C. parvum IMPDH inhibitors; Sel, ratio of the values of

Figure 16 (Table 10) depicts the effect in Q Series compounds of substitution on the carbon adjacent to the amide on inhibition of C. parvum/C. hominis IMPDH. IC ₅₀, inhibition of C. parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T. gondii strain expressing endogenous T. gondii IMPDH that should be resistant to C. parvum IMPDH inhibitors; CpIMPDH, a T. gondii strain that relies on the expression of C. parvum IMPDH that should be sensitive to C. parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀.

Figure 17 (Table 11) depicts the optimization of Q Series compounds for inhibition of C. parvum/C. hominis IMPDH. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T. gondii strain expressing endogenous T. gondii IMPDH that should be resistant to C. parvum IMPDH inhibitors; CpIMPDH, a T. gondii strain that relies on the expression of C. parvum IMPDH that should be sensitive to C. parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀. Figure 18 (Table 12) depicts the optimization of the naphthalene ring Q Series compounds for inhibition of C. parvum/C. hominis IMPDH. IC ₅₀, inhibition of C. parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T. gondii strain expressing endogenous T. gondii IMPDH that should be resistant to C. parvum IMPDH inhibitors; CpIMPDH, a T. gondii strain that relies on the expression of C. parvum IMPDH that should be sensitive to C. parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀.

Figure 19 (Table 13) depicts the results of biological screening of miscellaneous Q Series compounds for inhibition of C. parvum/C. hominis IMPDH. IC ₅₀, inhibition of C. parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T gondii strain expressing endogenous T gondii IMPDH that should be resistant to C parvum IMPDH inhibitors; CpIMPDH, a T gondii strain that relies on the expression of C parvum IMPDH that should be sensitive to C parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀.

Figure 20 (Table 14) depicts the SAR of a-methyl benzyl amide derivatives in the Q Series for inhibition of C parvum/C hominis IMPDH. IC ₅₀, inhibition of C parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T gondii strain expressing endogenous T gondii IMPDH that should be resistant to C parvum IMPDH inhibitors; CpIMPDH, a T gondii strain that relies on the expression of C parvum IMPDH that should be sensitive to C parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀.

Figure 21 (Table 15) depicts the SAR of secondary amine derivatives in the Q Series for inhibition of C parvum/C hominis IMPDH. IC ₅₀, inhibition of C parvum IMPDH activity. EC ₅₀, antiparasitic activity in a Toxoplasma gondii model of Cryptosporidium infection. WT, a T gondii strain expressing endogenous T gondii IMPDH that should be resistant to C parvum IMPDH inhibitors; CpIMPDH, a T gondii strain that relies on the expression of C parvum IMPDH that should be sensitive to C parvum IMPDH inhibitors; Sel, ratio of the values of EC ₅₀.

Figure 22 (Table 16) depicts the mouse microsomal stability of selected Q Series compounds. Figure 23 depicts biological data obtained in mouse models for selected P Series compounds. The table summarizes a preliminary analysis; an updated analysis is shown in Figure 31.

Figure 24 depicts for selected D, P, and Q Series compounds (i) data on inhibition of representative bacterial IMPDHs and (ii) antibacterial activity.

Figure 25 depicts the inhibition of C/?IMPDH by a compound of the invention.

Figure 26 depicts the effects of other miscellaneous structural changes, to explore the SAR of compounds of the invention, on Q?IMPDH inhibition.

Figure 27 depicts (a) inhibition of CpIMPDH by a compound of the invention. Inhibitor concentrations: open squares, 630 nM; closed squares, 380 nM; closed triangles, 130 nM; open triangles 50 nM; open circles, 25 nM; no inhibitor, closed circles; and (b) a table summarizing data gathered in the investigation of the mechanism of inhibition of Q?IMPDH by selected compounds of the invention. °NC, noncompetitive; C, competitive, ^compound was analyzed using tight binding treatment.

Figure 28 tabulates NADPH-dependent mouse liver microsomal stability for various compounds of the invention. °Estimated from a single time point at 45 min.

Figure 29 tabulates the antiparasitic activity of exemplary benzoxazole compounds of the invention. ^Ω Assays as described in Experimental Section. Unless otherwise stated all values are the average of three independent determinations. ^Selectivity is the ratio of EC ₅₀ of Toxo/Q?IMPDH to EC ₅₀ of Toxo/WT. ^cTwo determinations.

Figure 30 tabulates the physiochemical properties and performance in preliminary assays of various urea compounds of the invention, a. Calculated with ChemDraw software (Cambridgesoft). b. IC ₅₀ > 5 μΜ versus human IMPDH2 in all cases, c. Values from literature, d. Values from literature, e. Antiparasitic activity was assayed in a Toxoplasma gondii strain ( T. gondii/ CpIMPDH) engineered to rely on Q?IMPDH for the production of guanine nucleotides. Wild-type T. gondii contains an eukaryotic enzyme similar to human IMPDH that should be resistant to CpIMPDH inhibitors. Selectivity is the ratio of the values of EC ₅₀ for wild-type and T. gondii! CpIMPDH. High selectivity confirms that the compounds target Q?IMPDH and serves as a proxy for host cell effects, f. Values from literature, g. HCT-8 host cells, h. High content imaging assay, 27% inhibition at 12.5 μΜ, 83% inhibition at 25 μΜ. i. High content imaging assay, same antiparasitic activity as 1 mM paromomycin, j. MDCK host cells, k. No metabolism observed over 45 min. Assuming conservatively that 5% is the minimum metabolism reliably observed, then the lower limit of Ti _/2 is 600 min.

Figure 31 tabulates the activity of urea Q?IMPDH inhibitors in the IL-2 knockout mouse model of cryptosporidiosis. Infection protocol A: IL-12 knockout mice were infected with 1000 oocysts on day 1. Mice were treated daily by oral gavage beginning 4 hours after infection. Feces were collected and counted on day 7 unless otherwise noted. Vehicle = 5% DMSO/corn oil unless otherwise noted. Infection protocol B: IL-12 knockout mice were infected with 10,000 oocysts on day 1. Mice were treated three times daily by oral gavage beginning 4 hours after infection. Feces were collected and counted on day 4. Vehicle = 5% DMSO/corn oil unless otherwise noted. PRM = paromomycin, 2000 mg/kg-d. PRM data are shown in Figure 41. a. Vehicle = 20% PEG. b. Several mice lost weight on day 4 and looked ruffled day 7. These animals were not sampled, c. Vehicle = 10% PEG. d. Fecal oocysts counted on day 6 post infection. Significance was computed with the Mann Whitney nonparametric test using VassarStats. f. Significance was computed with a one tailed type 3 T-test (Microsoft Excel), g. removed outlier = 16,990 from P131 dataset. Figure 31 is an updated, corrected version of Figure 23.

Figure 32 tabulates the pharmacokinetic parameters for urea Q?IMPDH inhibitors. C57BL/6 mice received a single dose of 250 mg/kg in 10%> DMSO/corn oil by oral gavage. Mouse activity: +, significant antiparasitic in at least one trial; -, no antiparasitic activity; - (!), promotes parasitemia. *, measured levels of P82 generated by metabolism of P83, n.d., not determined.

Figure 33 tabulates urea Q?IMPDH inhibitor permeability and uptake in Caco-2 TC7 cells. All compounds at 10 μΜ, pH 7.4 on both sides. Solid recovery > 80%> in all cases, a. Approximate intracellular concentration, assuming 4 of water for 1 mg of cellular protein.

Figure 34 tabulates the effects of vehicle and P131 on fecal microbiota. Mice (10 per group) were treated with single daily doses of vehicle or 250 mg/kg P131. Fecal samples were collected and amplicons of bacterial 16S rRNA gene were constructed, sequenced with 454 pyrosequencing and analyzed with the QIIME platform. The relative abundance of phyla is shown as the percentage and SEM of total reads, a. n =4; b. n = 9. Figure 35 depicts antiparasitic activity of Q?IMPDH inhibitors. Each panel reflects a separate experiment. A, B and C) IL-12 knockout mice were infected with 1,000 C. parvum oocysts and treated with single daily doses of vehicle, paromomycin (2000 mg/kg) or compound by oral gavage. All compounds, with the exception of A110, were dosed at 250 mg/kg. A110 was dosed at 100 mg/kg. Fecal oocysts were counted on day 7 post infection. A) Experiment 2; B) Experiment 5; C) Experiment 7; D) Experiment 9; IL- 12 knockout mice were infected with 10,000 C. parvum oocysts and treated with three daily doses of either vehicle, paromomycin (670 mg/kg) or P131 (83 mg/kg). Fecal oocysts were counted on day 5 post infection.

Figure 36 depicts the concentration of P131 in various tissues 24 h after the first 83 mg/kg dose. Concentration in tissue is reported assuming 1 g of tissue is equivalent to 1 mL of liquid. An alternative way to express concentration is nmol/g.

Figure 37 depicts the amount of P131 remaining in Caco-2 cells after the cells were loaded with different concentrations of P131 for 2 h at 37 °C in HBSS. The efflux tm is ~ 1 h for loading at 100 μΜ and 2 h for 20 μΜ and 5 μΜ.

Figure 38 depicts effects of vehicle, A110 and P131 on gut microbiota. IL-12 knockout mice were treated with vehicle, A110 or P131 (250 mg/kg) for seven days. Each treatment group contained 10 mice. Fecal samples were collected and the 16S rRNA gene content was sequenced using 454 pyrosequencing and analyzed with the QIIME platform. A) The change in phyla distribution of the three treatment groups is shown. Statistical analysis was performed using Mann-Whitney U test (*** = P<0.001). B) and C) Beta diversity plots to compare the bacterial taxa between the treatments. Unweighted UniFrac (B) or weighted UniFrac (C) were used to generate a matrix of pairwise distances between communities and scatter plots were generated from matrix of distances using Principal Coordinate Analysis (PC A). The p values generated from the original UniFrac Matrix using QIIME scripts.

Figure 39 depicts antiparasitic activity of Q?IMPDH inhibitors. IL-12 knockout mice were infected with 1,000 C. parvum oocysts and treated with single daily doses of vehicle, paromomycin (2000 mg/kg) or compound (250 mg/kg) by oral gavage. Fecal oocysts were counted on day 7 post infection. Each panel displays a separate experiment.

Figure 40 depicts the effect of vehicle, A110 and P131 on fecal microbiota at the species level. The cumulative relative distribution of species in different treatments is listed. Figure 41 tabulates paromomycin controls for C. parvum infections of IL-12 knockout mice. Infection Protocol A was used unless otherwise noted. Infection protocol A: IL12 knockout mice were infected with 1000 oocysts on day 1. Mice were treated daily by oral gavage beginning 4 hours after infection. Feces were collected and counted on day 7 unless otherwise noted. Vehicle = 5% DMSO/corn oil unless otherwise noted. PRM = paromomycin, 2000 mg/kg-d. Infection protocol B: IL-12 knockout mice were infected with 10,000 oocysts on day 1. Mice were treated three times daily by oral gavage beginning 4 hours after infection. Feces were collected and counted on day 4. Vehicle = 5% DMSO/corn oil unless otherwise noted. PRM = paromomycin, 200 mg/kg-d. Significance was computed with the Mann Whitney nonparametric test using VassarStats and with a one tailed type 3 T-test (Microsoft Excel), a. Vehicle = 20% PEG; b. Vehicle = 10% PEG, DPI 6.

Figure 42 depicts the correlation of antibacterial activity and enzyme inhibition, (a) B.anthracis. (b) F. tularensis Schu S4. Open circles denote compounds where potency does not change with deletion of the guaB gene.

Figure 43 tabulates the minimum inhibitory concentration (MIC) for bacterial growth for various compounds of the invention. Ab, Acinetobacter baumannii ATCC 17961; Ba, Bacillus anthracis Sterne 7702; Be, Burkholderia cenocepacia K56-2; B. thailandensis E264; Cp, Campylobacter jejuni 81-176, Ft, Francisella tularensis Schu S4; Hp, Helicobacter pylori; Sa, Staphylococcus aureus NCTC 8325; Lm, Listeria monocytogenes 10403S; Mtb, Mycobacterium tuberculosis H37Rv. No compounds (0/148) displayed antibacterial activity against P. aeruginosa PA14. Gray box denotees MIC > 12 μΜ. * antibacterial activity observed at 50 μΜ in Mueller-Hinton II cation adjusted broth; MIC in progress. ** only listed compounds with MIC < 6 μΜ unless also active against a second bacteria.

Figure 44 depicts the structures of various compounds of the invention. Figure 45 depicts the structures of various compounds of the invention.

DETAILED DESCRIPTION

Overview

One aspect of the present invention relates to compounds and pharmaceutically acceptable salts thereof, which are useful as inhibitors of IMPDH. In certain embodiments, a compound of the invention selectively inhibits a parasitic or bacterial IMPDH versus a host (e.g., mammalian) IMPDH. In certain embodiments, the present invention relates to selective inhibition of Cryptosporidium IMPDH in the presence of human inosine-5'- monophosphate-dehydrogenase (IMPDH type I and type II). Further, the invention provides pharmaceutical compositions comprising one or more compounds of the invention. The invention also relates to methods of treating various parasitic and bacterial infections in mammals. Moreover, the compounds may be used alone or in combination with other therapeutic or prophylactic agents, such as anti-virals, anti-inflammatory agents, antimicrobials and immunosuppressants.

Selective Inhibition of Microbial IMPDH. IMPDH enzymes are also known to be present in bacteria and protozoans and thus may regulate microbial growth. As such, the IMPDH-inhibitor compounds, compositions and methods described herein may be useful as antibacterials and antiprotozoans, either alone or in combination with other anti-microbial agents.

Enzyme inhibition can be measured by various methods, including, for example, IMPDH HPLC assays (measuring enzymatic production of XMP and NADH from IMP and NAD ⁺), IMPDH spectrophotometric assays (measuring enzymatic production of NADH from NAD ⁺ or XMP from IMP), IMPDH fluorometric assays (measuring enzymatic production of NADH from NAD ⁺), IMPDH radioassays (measuring enzymatic production of radiolabeled XMP from radiolabeled IMP or tritium release into water from 2- ³H-IMP). [See C. Montero et al, Clinica Chimica Acta, 238, pp. 169-178 (1995)]. Additional assays known in the art can be used in ascertaining the degree of activity of an inventive compound as an IMPDH inhibitor. For example, activity of IMPDH I and IMPDH II can be measured following an adaptation of the method described in WO 97/40028. [See, additionally, U.S. Patent Application 2004/0102497 (incorporated by reference)].

Accordingly, in certain embodiments, the inventive compounds are capable of targeting and selectively inhibiting the IMPDH enzyme in bacteria. It is known that knocking out the IMPDH gene makes some bacteria avirulent, while has no effect on others. The effectiveness probably depends on which salvage pathways are operational in a given bacteria, and the environmental niche of the infection. It has been shown that IMPDHs from Helicobacter pylori, Streptococcus pyogenes, Borrelia burgdorferi are sensitive to inhibitors of C. parvum IMPDH, and that the growth of H pylori can be blocked by C. parvum IMPDH inhibitors [Gollapalli et al, Chem. Biol, 17, 1084-1091 (2010)]. We have also shown IMPDHs from Bacillus anthracis, Burkholderia mallei/pseudomallei, Listeria monocytogenes, Francisella tularensis, Acinetobacter baumannii, Staphylococcus aureus, Pseudomonas aeruginosa, Campylobacter jejuni and Clostridia perfringes are inhibited by compounds that inhibit C parvum IMPDH [Makowska-Gryska et al. Biochem. 2012, 51, 6148-6163]. We have also shown antibacterial activity of at least one C. parvum IMPDH inhibitor against Ba. anthracis, F. tularensis, Sta. aureus, P. aeruginosa, C. perfringes, L. monocytogenes and Mycobacterium tuberculosis. It is also expected that various Campylobacter, Arcobacter, Bacteroides, Coxiella, Pseudomonas, Fusobacterium, Brucella, Burkholderia, Brachyspira, Clostridia, Neisseria, Mycobacterium, or Acinetobacter organisms will be inhibited by the compounds described herein. Organisms belonging to these genera are responsible for illnesses such as ulcers and acid reflux (H. pylori), anthrax (Ba. anthracis), Lyme disease (B. burgdorferi), brucellosis (Br. abortus), infection (S. pyogenes), food poisoning (Ca. jejuni and Ar. butzleri), abscesses (Bact. capillosis), periodontitis (F. nucleatum), skin ulcers (F. nucleatum), Lemierre's syndrome (F. nucleatum), infection in cystic fibrosis (Bu. cenocepacia), pneumonia (Str. pneumoniae), botulism (CI. botulinum), gonorrhea (TV. gonorrhoeae), Q fever (Co. burnetii) tuberculosis (M. tuberculosis), leprosy (M. leprae), opportunistic infection (Ps. aeruginosa) and drug resistant infection (A. baumannii).

Further, in certain embodiments, the inventive compounds are capable of targeting and selectively inhibiting the IMPDH enzyme in protozoans, such as Cryptosporidium, Entamoeba, Leishmania and Trypanosoma. In certain embodiments, these compounds are capable of targeting and selectively inhibiting the IMPDH enzyme in Cryptosporidium parvum/hominis and other Cryptosporidium species.

Selected Compounds of the Invention Phthalazinone Series

In certain embodiments, the invention relates to a compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula I:

Formula I wherein, independently for each occurrence,

R ¹ is alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, haloalkyl, aralkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, formyl, acyl, aracyl, heteroaracyl, carboxyl, alkoxycarbonyl, acyloxy, cyano, -OR', halide, -N(R') ₂, azido, nitro, amido, isocyano, phosphonate, phosphinate, silyl, thio, alkylthio, sulfonate, sulfonyl, sulfonamido, or sulfhydryl; wherein R' is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl;

R ² is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy;

R ³ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; m is 0, 1, or 2; n is 1-4; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein any of the aforementioned alkyl, aryl, heteroaryl, or aralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ¹ is alkyl.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ¹ is halogen.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ¹ is OH or methoxy.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ² is hydrogen.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ² is alkyl.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ² is methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, i-butyl, or t- butyl.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein R ³ is hydrogen.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein m is 1.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein n is 1.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein n is 2. In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein is alkyl, benzyl,

aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, haloalkyloxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, alkylthio, sulfonate, sulfonyl, sulfonamido, formyl, cyano, or isocyano.

In certain embodiments, the invention relates to any one of the aforementioned compounds, wherein n is 2 and R ¹ is halogen.

In certain embodiments, the invention relates to any one of the aforementioned

compound amido, alkoxy, halo, haloalkyl, aryl haloaryl, alkyl, hydroxy, alkylthio, sulfonyl, haloalkoxy, or cyano.

In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein is ; and R is alkoxy or halo.

In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein is ; and R ⁵ is halo.

In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein is and R ⁵ is halo or cyano.

In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein ; and R ⁵ is amido, halo, or cyano.

In certain embodiments, the invention relates to any one of the aforementioned

compound halo. In certain embodiments, the invention relates to any one of the aforementioned

compounds, wherein is ^RJO ; and R is amido.

In certain embodiments, the invention relates to a compound, or a pharmaceutically acceptable salt or ester thereof, represented by Formula II:

Formula II wherein, independently for each occurrence,

R ¹ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, haloalkyl, aralkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, formyl, acyl, aracyl, heteroaracyl, carboxyl, alkoxycarbonyl, acyloxy, cyano, -OR', halide, -N(R') ₂, azido, nitro, amido, isocyano, phosphonate, phosphinate, silyl, thio, alkylthio, sulfonate, sulfonyl, sulfonamido, or sulfhydryl; wherein R' is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, or heteroaracyl;

R ² is alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy;

R ³ is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, acyl, aracyl, heteroaracyl sulfonate, sulfonyl, sulfonamido, formyl, carboxyl, alkoxycarbonyl, or acyloxy; m is 0, 1, or 2; n is 1-4; and is alkyl, monocyclic or bicyclic cycloalkyl, monocyclic or bicyclic heterocyclyl, monocyclic or bicyclic aryl or monocyclic or bicyclic heteroaryl; wherein, any of the aforementioned alkyl, aryl, heteroaryl, or aralkyl may be substituted with one or more groups independently selected from the group consisting of halo, azido, alkyl, haloalkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, heteroaralkyl, hydroxy, alkoxy, aryloxy, heteroaryloxy, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, acyl, carboxyl, alkoxycarbonyl, acyloxy, silyl, alkylthio, sulfonate, sulfonyl, sulfonamido, sulfhydryl, formyl, cyano, and isocyano.

In certain embodiments, the invention relates to any of the aforementioned compounds, wherein R ¹ is hydrogen.