Title:
CONSTRUCT AND METHOD FOR THE INTERNALIZATION OF CARGO MOLECULES INTO A CELL
Document Type and Number:
WIPO Patent Application WO/2010/010112
Kind Code:
A3
Abstract:
The present invention relates to the delivery of molecules into cells. Especially, it relates to a carrier complex comprising (i) a molecule comprising a receptor peptide and a peptide which is capable of penetrating a host cell; and (ii) a second molecule comprising a ligand peptide and a cargo molecule of interest. In a preferred embodiment, the carrier complex comprises (i) a fusion protein between the TAT-peptide of HIV I and streptactin, a streptavidin mutant; and (ii) a conjugate or a fusion protein between the Strep-tag II, a streptavidin-binding peptide, and the cargo molecule to be delivered.
Inventors:
MOOSMEIER MARKUS (DE)
HOPPE-SEYLER FELIX (DE)
HOPPE-SEYLER KARIN (DE)
HOPPE-SEYLER FELIX (DE)
HOPPE-SEYLER KARIN (DE)
Application Number:
PCT/EP2009/059411
Publication Date:
June 24, 2010
Filing Date:
July 22, 2009
Export Citation:
Assignee:
DKFZ KREBSFORSCHUNGSZENTRUM (DE)
MOOSMEIER MARKUS (DE)
HOPPE-SEYLER FELIX (DE)
HOPPE-SEYLER KARIN (DE)
MOOSMEIER MARKUS (DE)
HOPPE-SEYLER FELIX (DE)
HOPPE-SEYLER KARIN (DE)
International Classes:
A61K47/48; A61P3/00; A61P9/00; A61P25/00; A61P29/00; A61P31/00; A61P35/00; A61P37/00
Foreign References:
| US20050002945A1 | 2005-01-06 | |||
| US20060281677A1 | 2006-12-14 |
Other References:
PAVLINKOVA G ET AL: "Constructs of biotin mimetic peptide with CC49 single-chain Fv designed for tumor pretargeting", PEPTIDES 20030301 US, vol. 24, no. 3, 1 March 2003 (2003-03-01), pages 353 - 362, XP002507569, ISSN: 0196-9781
SKERRA A ET AL: "Applications of a peptide ligand for streptavidin: the Strep-tag", BIOMOLECULAR ENGINEERING, ELSEVIER, NEW YORK, NY, US, vol. 16, no. 1-4, 31 December 1999 (1999-12-31), pages 79 - 86, XP004257802, ISSN: 1389-0344
LAMLA T ET AL: "The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 33, no. 1, 1 January 2004 (2004-01-01), pages 39 - 47, XP004475011, ISSN: 1046-5928
KEEFE A D ET AL: "One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-tag", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 23, no. 3, 1 December 2001 (2001-12-01), pages 440 - 446, XP002253300, ISSN: 1046-5928
SCHMIDT THOMAS G M ET AL: "Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 255, no. 5, 1 January 1996 (1996-01-01), pages 753 - 766, XP002209213, ISSN: 0022-2836
SKERRA A ET AL: "Applications of a peptide ligand for streptavidin: the Strep-tag", BIOMOLECULAR ENGINEERING, ELSEVIER, NEW YORK, NY, US, vol. 16, no. 1-4, 31 December 1999 (1999-12-31), pages 79 - 86, XP004257802, ISSN: 1389-0344
LAMLA T ET AL: "The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 33, no. 1, 1 January 2004 (2004-01-01), pages 39 - 47, XP004475011, ISSN: 1046-5928
KEEFE A D ET AL: "One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-tag", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 23, no. 3, 1 December 2001 (2001-12-01), pages 440 - 446, XP002253300, ISSN: 1046-5928
SCHMIDT THOMAS G M ET AL: "Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 255, no. 5, 1 January 1996 (1996-01-01), pages 753 - 766, XP002209213, ISSN: 0022-2836
Attorney, Agent or Firm:
HERZOG FIESSER & PARTNER et al. (Mannheim, DE)
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