Title:
CONSTRUCTION METHOD AND APPLICATION OF MICROORGANISM HAVING HIGH LACTO-N-NEOTETRAOSE PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2023/011577
Kind Code:
A1
Abstract:
A construction method and an application of a microorganism having high lacto-N-neotetraose production, belonging to the field of microorganism genetic engineering. On the basis of strains having related genes knocked out, genes encoding β-1,3-acetylglucosaminyltransferase, β-1,4-galactosyltransferase and/or UDP-glucose 4-epimerase are overexpressed, giving them an ability to produce lacto-N-tetraose. By means of screening high-efficiency β-1,4-galactosyltransferase genes and combinatorial regulation of expression of lgtA, Aa-β-1,4-GalT and galE in a lacto-N-neotetraose synthesis pathway, carbon flux of the metabolic pathway can be precisely regulated and metabolic pressure can be relieved. In a shake flask experiment, the ability of Escherichia coli to produce lacto-N-neotetraose is 0.91 g/L, and in a 3L fermentation tank, the yield of lacto-N-neotetraose can reach 12.14 g/L.
Inventors:
MU WANMENG (CN)
ZHANG WENLI (CN)
ZHU YINGYING (CN)
LUO GUOCONG (CN)
ZHANG WENLI (CN)
ZHU YINGYING (CN)
LUO GUOCONG (CN)
Application Number:
PCT/CN2022/110193
Publication Date:
February 09, 2023
Filing Date:
August 04, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/12; C12N1/21; C12N9/10; C12N9/38; C12N15/70
Domestic Patent References:
| WO2015150328A1 | 2015-10-08 | |||
| WO2007023348A2 | 2007-03-01 |
Foreign References:
| CN113684164A | 2021-11-23 | |||
| CN113136357A | 2021-07-20 | |||
| CN108410787A | 2018-08-17 | |||
| CN112280727A | 2021-01-29 | |||
| CN111979168A | 2020-11-24 |
Other References:
DATABASE Protein NCBI; 7 October 2016 (2016-10-07), ANONYMOUS : "lipooligosaccharide biosynthesis protein lex-1 [Aggregatibacter actinomycetemcomitans NUM4039]", XP093032825, Database accession no. BAS48030
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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