Title:
CONSTRUCTION METHOD AND USE OF METABOLICALLY ENGINEERED E. COLI STRAINS FOR PRODUCING SUCCINIC ACID BY USING ACETIC ACID
Document Type and Number:
WIPO Patent Application WO/2016/065709
Kind Code:
A1
Abstract:
Disclosed are the construction method and application of metabolically engineered E. coli strains for producing succinic acid by using acetic acid, wherein succinic acid is produced from the metabolically engineered modified E. coli by fermentation using acetic acid as a raw material, and the transformation pathway is blocking the TCA cycle, and/or blocking the succinic acid utilization pathway, and/or enhancing the acetic acid uptake and oxaloacetate supply, strengthening the glyoxylate cycle, and/or deleting the by-product formation pathway, and/or reducing the ineffective circulation caused by the producing acetic acid from pyruvic acid through decarboxylation, and/or dredging the acetyl CoA node metabolic flux. Through analysis of the metabolic pathways and regulation thereof, E. coli strains are modified by using genetic engineering pathways, and the obtained strains are able to produce succinic acid in a culture medium using acetic acid as the carbon source, with no by-product produced.
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Inventors:
LI ZHIMIN (CN)
LI YUNJIE (CN)
WU HUI (CN)
YE QIN (CN)
LI YUNJIE (CN)
WU HUI (CN)
YE QIN (CN)
Application Number:
PCT/CN2014/094215
Publication Date:
May 06, 2016
Filing Date:
December 18, 2014
Export Citation:
Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C12P7/46; C12N1/21
Domestic Patent References:
| WO2006031424A2 | 2006-03-23 |
Foreign References:
| CN101363032A | 2009-02-11 | |||
| CN102174455A | 2011-09-07 | |||
| CN101792778A | 2010-08-04 |
Other References:
WANG, YI'NA ET AL.: "Effects of Carbon Sources on Succinate Production by Dual-phase Fermentations of Recombinant Escherichia Coli", CHINA BIOTECHNOLOGY, vol. 29, no. 3, 31 March 2009 (2009-03-31), pages 57 - 62
Attorney, Agent or Firm:
SHANGHAI ESSEN PATENT&TRADEMARK AGENCY (CN)
上海翼胜专利商标事务所（普通合伙） (CN)
上海翼胜专利商标事务所（普通合伙） (CN)
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