Title:
CONSTRUCTS AND SYSTEMS AND METHODS FOR ENGINEERING A CO2 FIXING PHOTORESPIRATORY BY-PASS PATHWAY
Document Type and Number:
WIPO Patent Application WO/2015/120343
Kind Code:
A3
Abstract:
Methods and compositions to introduce a synthetic pathway based on the 3- hydroxypropionate bicycle into an organism such as the model cyanobacterium, Synechococcus elongatus sp. PCC 7942. The heterologously expressed pathway acts as a photorespiratory bypass as well as an additional carbon fixation cycle orthogonal to the endogenous Calvin-Benson cycle. We demonstrate the function of all six introduced enzymes, which not only has implications on increasing net-photosynthetic productivity, but also key enzymes in the pathway are involved in high-value products that are of biotechnological interest, such as 3-hydroxypropionate.
Inventors:
KERFELD CHERYL A (US)
SHIH PATRICK (US)
ZARZYCKI JAN (US)
NIYOGI KRISHNA K (US)
SHIH PATRICK (US)
ZARZYCKI JAN (US)
NIYOGI KRISHNA K (US)
Application Number:
PCT/US2015/014929
Publication Date:
October 15, 2015
Filing Date:
February 06, 2015
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
KERFELD CHERYL A (US)
SHIH PATRICK (US)
ZARZYCKI JAN (US)
NIYOGI KRISHNA K (US)
KERFELD CHERYL A (US)
SHIH PATRICK (US)
ZARZYCKI JAN (US)
NIYOGI KRISHNA K (US)
International Classes:
C12N1/00; C12N1/21; C12N15/03; C12N15/05; C12N15/09; C12P1/00
Domestic Patent References:
| WO2013130394A1 | 2013-09-06 |
Foreign References:
| US8349587B2 | 2013-01-08 |
Other References:
ZARZYCKI, J ET AL.: "Identifying The Missing Steps Of The Autotrophic 3-Hydroxypropionate C02 Fixation Cycle In Chloroflexus aurantiacus", PROC. NATL. ACAD. SCI. USA., vol. 106, no. 50, 15 December 2009 (2009-12-15), pages 21317 - 21322, XP055085882
WANG ET AL.: "Biosynthetic Pathway For Poly(3-Hydroxypropionate) In Recombinant Escherichia coli.", J. MICROBIOL., vol. 50, no. 4, 2012, pages 693 - 697, XP035102848
WANG ET AL.: "Biosynthetic Pathway For Poly(3-Hydroxypropionate) In Recombinant Escherichia coli.", J. MICROBIOL., vol. 50, no. 4, 2012, pages 693 - 697, XP035102848
Attorney, Agent or Firm:
WONG, Michelle, Chew (Technology Transfer and Intellectual Property Mgmt Dept.One Cyclotron Road, MS 56A-012, Berkeley CA, US)
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