Title:
CONTAINER FOR SPERM CRYOPRESERVATION USE AND METHOD FOR PRODUCING SAME, SPERM CRYOPRESERVATION METHOD, AND IN VITRO FERTILIZATION METHOD
Document Type and Number:
WIPO Patent Application WO/2017/007006
Kind Code:
A1
Abstract:
Provided are: a container for sperm cryopreservation use, which makes it possible to perform the cryopreservation and collection of sperms effectively even when the number of the sperms is small; a method for producing the container; a sperm cryopreservation method; and an in vitro fertilization method. The sperm cryopreservation method according to one embodiment of the present invention comprises the following steps (a) to (f): (a) providing a container (10) which is provided with a gel shell (11) containing a glycosaminoglycan chain and/or a cellulose chain and a liquid core (12) coated on the gel shell (11); (b) injecting sperms (S) into the liquid core in the container to obtain the container having the sperms contained therein; (c) cryopreserving the container having the sperms contained therein; (d) thawing the cryopreserved container; (e) treating the gel shell in the thawed container with an enzyme capable of decomposing the glycosaminoglycan chain and/or an enzyme capable of decomposing the cellulose chain to decompose at least a part of the gel shell; and (f) collecting the sperms from the container after the decomposition of the gel shell.
Inventors:
SAKAI SHINJI (JP)
TAYA MASAHITO (JP)
TOMITA KAZUHISA (JP)
HOSOI YOSHIHIKO (JP)
TAYA MASAHITO (JP)
TOMITA KAZUHISA (JP)
HOSOI YOSHIHIKO (JP)
Application Number:
PCT/JP2016/070194
Publication Date:
January 12, 2017
Filing Date:
July 07, 2016
Export Citation:
Assignee:
UNIV OSAKA (JP)
UNIV KINKI (JP)
UNIV KINKI (JP)
International Classes:
C12N5/076; A01N1/02; A61D19/02
Foreign References:
| JP2007530052A | 2007-11-01 | |||
| JP2010042027A | 2010-02-25 | |||
| JP2012533620A | 2012-12-27 |
Other References:
WEBER W. ET AL.: "Design of high-throughput- compatible protocols for microencapsulation, cryopreservation and release of bovine spermatozoa.", JOURNAL OF BIOTECHNOLOGY, vol. 123, 2006, pages 155 - 163, XP024956801
SAKAI S. ET AL.: "Enzymatically fabricated and degradable microcapsules for production of multicellular spheroids with well-defined diameters of less than 150 pm.", BIOMATERIALS, vol. 30, 2009, pages 5937 - 5942, XP026524658
SAKAI S. ET AL.: "Production of cell -enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 99, no. 1, 2008, pages 235 - 243, XP055050181
YASUYUKI ARAKI ET AL.: "Agarose Capsule o Mochiita Kyokusho Hito Seishi Toketsu no Kokoromi", JOURNAL OF JAPAN SOCIETY FOR REPRODUCTIVE MEDICINE, vol. 59, no. 4, 2014, pages 336
SAKAI S. ET AL.: "Peroxidase-catalyzed cell encapsulation in subsieve-size capsules of alginate with phenol moieties in water- immiscible fluid dissolving H2O2.", BIOMACROMOLECULES, vol. 8, 2007, pages 2622 - 2626, XP055345329
SAKAI S. ET AL.: "Enzymatically fabricated and degradable microcapsules for production of multicellular spheroids with well-defined diameters of less than 150 pm.", BIOMATERIALS, vol. 30, 2009, pages 5937 - 5942, XP026524658
SAKAI S. ET AL.: "Production of cell -enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 99, no. 1, 2008, pages 235 - 243, XP055050181
YASUYUKI ARAKI ET AL.: "Agarose Capsule o Mochiita Kyokusho Hito Seishi Toketsu no Kokoromi", JOURNAL OF JAPAN SOCIETY FOR REPRODUCTIVE MEDICINE, vol. 59, no. 4, 2014, pages 336
SAKAI S. ET AL.: "Peroxidase-catalyzed cell encapsulation in subsieve-size capsules of alginate with phenol moieties in water- immiscible fluid dissolving H2O2.", BIOMACROMOLECULES, vol. 8, 2007, pages 2622 - 2626, XP055345329
Attorney, Agent or Firm:
HARUKA PATENT & TRADEMARK ATTORNEYS (JP)
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