Title:
CONTROL SEQUENCES RESPONDING TO AMP AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2006066588
Kind Code:
A3
Abstract:
The present invention relates to a method of identifying control sequences responding to an-timicrobial peptides (AMPs), to host cells expressing an AMP and comprising a control se-quence of the invention operable linked to a reporter, and to a method of screening for novel AMPs or AMP-variants having improved antimicrobial activity. Particularly the present inven-tion relates to DNA control sequences, wherein the transcriptional profiles of said control se-quences are regulated by the presence of an antimicrobial polypeptide expressed inside the host cell.
Inventors:
NIELSEN JESPER DUUS (DK)
NIELSEN ALLAN KENT (DK)
CHRISTENSEN BJARKE (DK)
PEDERSEN POUL ERIK (DK)
HOEGENHAUG HANS-HENRIK KRISTEN (DK)
NIELSEN ALLAN KENT (DK)
CHRISTENSEN BJARKE (DK)
PEDERSEN POUL ERIK (DK)
HOEGENHAUG HANS-HENRIK KRISTEN (DK)
Application Number:
PCT/DK2005/000807
Publication Date:
August 24, 2006
Filing Date:
December 20, 2005
Export Citation:
Assignee:
NOVOZYMES AS (DK)
NIELSEN JESPER DUUS (DK)
NIELSEN ALLAN KENT (DK)
CHRISTENSEN BJARKE (DK)
PEDERSEN POUL ERIK (DK)
HOEGENHAUG HANS-HENRIK KRISTEN (DK)
NIELSEN JESPER DUUS (DK)
NIELSEN ALLAN KENT (DK)
CHRISTENSEN BJARKE (DK)
PEDERSEN POUL ERIK (DK)
HOEGENHAUG HANS-HENRIK KRISTEN (DK)
International Classes:
C12Q1/68; C12N15/10
Domestic Patent References:
| WO1999045152A1 | 1999-09-10 | |||
| WO2004033715A1 | 2004-04-22 | |||
| WO2004022720A2 | 2004-03-18 | |||
| WO2000073433A1 | 2000-12-07 |
Other References:
TOMASINSIG LINDA ET AL: "Genome-wide transcriptional profiling of the Escherichia coli response to a proline-rich antimicrobial peptide.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. SEP 2004, vol. 48, no. 9, September 2004 (2004-09-01), pages 3260 - 3267, XP002385101, ISSN: 0066-4804
HONG ROBERT W ET AL: "Transcriptional profile of the Escherichia coli response to the antimicrobial insect peptide cecropin A.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. JAN 2003, vol. 47, no. 1, January 2003 (2003-01-01), pages 1 - 6, XP002385102, ISSN: 0066-4804
OH J T ET AL: "Cationic peptide antimicrobials induce selective transcription of micF and osmY in Escherichia coli.", BIOCHIMICA ET BIOPHYSICA ACTA. 15 JAN 2000, vol. 1463, no. 1, 15 January 2000 (2000-01-15), pages 43 - 54, XP002385103, ISSN: 0006-3002
HONG ROBERT W ET AL: "Transcriptional profile of the Escherichia coli response to the antimicrobial insect peptide cecropin A.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. JAN 2003, vol. 47, no. 1, January 2003 (2003-01-01), pages 1 - 6, XP002385102, ISSN: 0066-4804
OH J T ET AL: "Cationic peptide antimicrobials induce selective transcription of micF and osmY in Escherichia coli.", BIOCHIMICA ET BIOPHYSICA ACTA. 15 JAN 2000, vol. 1463, no. 1, 15 January 2000 (2000-01-15), pages 43 - 54, XP002385103, ISSN: 0006-3002
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