This application is a continuation-in-part of the following applications: Serial No. 07/572,492 entitled

DETOXIFICATION OF SOIL filed August 23, 1990 and Serial No.

07/855,079 entitled COMPOSITION AND METHOD OF TREATING SOIL filed March 23, 1992.

BACKGROUND OF THE INVENTION

This invention relates to a method and to compositions for the control of soil borne pests and pathogens of the animal kingdom. Ne atodes are an example of soil borne pathogens and pests which are the subject of this invention. Nematodes, e.g., the species Meloidogyne incognita, Pratylenehus penetrans, Criconemella xenoplax, Xiphinema americanum, Meloidogyne javanica, M. hapla, Pratylenehus vulnus, Paratylenchus ineus, Tylenchulus semipenetrans, Helicotylenchus spp. , Nacobbus aberrans, Heterodera spp. , Ditylenchus spp., Xiphinema index, Longidorus spp., Rhadopholus spp., are widely prevalent in soil and are harmful to crops grown in the soil. For example crops such as [field crops] : sugar beets, alfalfa, corn, cotton, wheat, safflower, sunflower, flax, soybeans, beans, peas, milo, barley, rape; [vegetable crops]: lettuce, tomato, carrots, beets, broccoli, spinach, cabbage, pepper, cucumber, onions, radish, potato, taro, pumpkins; [fruit and nuts crops]:

almonds, peaches, plums, pistachios, walnuts, apples, cherries, grapes, kiwi, fajoa, banana, cacao, guava, filberts, macadamia, oranges, lemons, grapefruit, nectarines, strawberries, raspberries, blueberries, cranberries; [ornamentals]: pine, redwood, fir, larch, birch, elm, alder, aspen, ash, flowering pear, flowering plum, jasmine, boxwood, rose, chrysanthemum, carnation, rhododendron, azalea, camellias, nandina, juniper, bluegrass, rye grass, fescue, ivy, are adversely affected by nematodes in the soil.

The soil borne pests and pathogens to which this invention is applicable also include arthropods, e.g.,:

Insects

The order Homoptera such as Grape Phylloxera (Daktulosphaira vitifoliae) ; Strawberry Root Aphid (Aphis forbesi) ; Sugar Beet Root Aphid (Pemphigus betae) ; and Woolly Apple Aphid (Eriosoma lanigerum) .

The order Coleoptera such as Strawberry Root

Weevil (Brachyrhinus ovatus) ; White Grubs (many species of Phyllophaga, Family Scarabacidae) ; Japanese Beetle (Popiliia japonica) ; and Wireworms (many species of family

Elateridae) .

The order Lepidoptera such as Cutworms (many species of the family Noctuidae) ; andArmyworms (Pseudaletia unipuncta) .

The order Diptera such as Root Maggots (many species of family Anthomyiidae) .

Mites

Soil mites (various species of order Acarina) .

Presently toxic materials are incorporated in the soil to eradicate such pathogens and pests or to diminish their harmful effects. For example, methyl bromide is commonly injected into the soil on which various crops are to be grown, such as strawberries, fruit and nut trees, vines,

onions, kiwi, seed carrots. Other fumigants include: Telone (1, 3-dichloropropene) , Vapam (metam-sodium) , Vorlex (methyl isothiocyanate) , carbon disulfide, chloropicrin, ethylene dibromide, dazomet, nemacur, furadan, vydate, temik, and mocap.

Such methods are expensive and they introduce the hazard of toxic materials escaping into the environment or being harmful to workers.

In a paper published in 1983 by Huebner and others, in the Journal Nematropica, Vol. 13, pgs. 37-54, the use of urea as a nematocide is described. The urea hydrolyzes to ammonia which is the effective nematocide. The urea is used in amounts that are phytotoxic. To inhibit this phytotoxic effect hemicellulosic waste is added to soil along with the urea. The hemicellulosic waste is that resulting from alkaline and bisulfite treatment of wood to release cellulose.

The method of Huebner, as stated above, uses urea as the active nematocide and hemicellulosic waste as an inhibitor of phytotoxicity. The amounts of both urea and hemicellulosic waste applied to the soil by Huebner are such that the urea is phytotoxic (but its use is said to be justified because of its nematocidal effect) and both the urea and the hemicullulosic waste are expensive in the amounts used.

OBJECTS OF THE INVENTION

It is an object of the invention to provide methods using compositions which are non-toxic or relatively non- toxic to human beings but which are effective to control infestation of the soil by pests and pathogens.

It is a further object of the invention to provide compositions for application to soil, and rates of application of such compositions to soil, which are not phytotoxic with respect to the crops grown in the soil (or which have at most an acceptable level of phytotoxicity)

but which are effective to eliminate, or greatly reduce the phytotoxicity of soil pathogens and pests.

The above and other objects of the invention will be apparent from the ensuing description in the appended claims.

SUMMARY OF THE INVENTION

In accordance with the invention soil contaminated or likely to be contaminated with soil borne pests or pathogens of the animal kingdom is treated with a substance which is relatively non-toxic and which functions when it comes into contact with pests or pathogens in the soil to be absorbed through the cuticles and to so affect the pseudocoelomic fluid as to render the pests or pathogens more amenable to attack by soil microbes such as soil fungi and soil bacteria.

Preferably this material is accompanied by a nutrient medium which serves to promote the growth of soil microbes such as fungi and soil bacteria. It also may be accompanied by the introduction of soil microbes which are grown separately from the soil being treated and which are known to be effective in controlling pests and pathogens.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The material used to inactivate or destroy the pests or pathogens is preferably a soluble lignosulfonate, e.g., calcium, potassium, sodium or ammonium lignosulfonate. Lignosulfonate is a product of the pulping of wood to produce pulp for the manufacture of paper. Lignin itself is known to be a complex randomly cross-linked polymer in which phenyl propane derivatives are the building blocks. See, for example the product brochure: "Lignosulfonate Products," Georgia Pacific Publication, 1754 Thorne Road, Tacoma, WA 98421. This lignin is found in woody tissues, e.g. , in soft woods such as pines. It is extracted from wood by a sulfite liquor and exists in the form of a sulfonate such as the calcium, ammonium, sodium or potassium

sulfonate which are water soluble. A calcium lignosulfonate of that company which is suitable is described in the company's literature as follows:

A 50% aqueous solution of high purity derived from soft wood and as having the following specification:

CHEMICAL DESCRIPTION

Total solids, % 50.0

Calcium lignin sulfonate, % 40.0

Methoxyl, % 4.2 Reducing sugars (as glucose), % 3.6

Calcium (soluble), % 2.5

Sodium, % 0.5

Insolubles (CaS0 ₄ 2H ₂ 0, % 1.5-2.0 pH of 10% solution 5.5

PHYSICAL PROPERTIES

Specific gravity (liquid, 25°/15° 1.252

Gallon weight, lbs 10.4

Heat of combustion, BTU/lb solids 8100

Viscosity, cp at 25°C 700 It is believed that the sulfonates, when incorporated in soil infected with pests or pathogens, e.g., nematodes, penetrate the cuticle of the organisms and enter the pseudocoelomic fluid of the organisms where it adversely affects the enzymes of the organisms. This in turn is believed to weaken the organisms resistance to invading soil bacteria leading to the destruction or inactivation of the organisms.

Whatever the mechanism I have discovered that lignosulfonate does in fact greatly mitigate or completely destroy the pathogenic effect of soil pests and pathogens.

The lignosulfonate may be dissolved in water which is then used by way of any of several methods for application to soil, e.g., by sprinkling, by drip methods, by field irrigation, by spraying the soil or by injection into the

soil followed by irrigation. The lignosulfonate may also be applied in dry form as by aerial dusting followed by irrigation to dissolve the lignosulfonate and cause it to penetrate the soil. The lignosulfonate may be applied uniformly over an area or it may be applied selectively in the vicinity of plants, seeds or seedlings. It may also be applied in pelletized form.

The lignosulfonate, produced as a byproduct of wood pulping, contains also certain nutrients, e.g., sugarswhich are useful nutrients for soil bacteria which in turn penetrate and destroy the soil pests and pathogens exposed to the lignosulfonate. It is advantageous, however, to supplement the lignosulfonate directly, or by adding to the soil treated with lignosulfonate, a more complete nutrient material comprising nitrogen and phosphorus macronutrients, also various other macro- and icronutrients together with a vitamin/co-factor such as yeast extract.

It may also be advantageous to supplement the naturally occurring soil bacteria with added bacteria or other microbes which effectively complement the action of the lignosulfonate.

Instead of lignosulfonates as the penetrant material, other materials may be used such as [1] aromatic a ino acids (e.g., tyrosine, phenylalanine, tryptophan, shikimic acid) ; [2] miscellaneous simplephenols and sister compounds (e.g. , chlorogenic acid, caffeic acid, cinnamic acid, coumaric acid, catechuic acid, ferulic acid, chorismic acid, quinic acid, gallic acid, gallotannins, scopeletin, dicoumarol, preocenes, phytoalexins such as orchinol, phaseolin, pisatin, isocoumarin) ; [3] cignin alcohols (e.g., conferyl, sinapyl, p-coumaryl) ; [4] flavanoids (e.g., cyanidin, anthocyanidin, pelargonidin, delphinidin, malidin, peonidin, petunidin) ; [5] flavonols and flavones; [6] betalains (e.g., betacyanin, betalain, betaxanthin) and [7] alkaloids (e.g., caffeine, nicotine, theobromine) ; [8] limonene (l-Methyl-4- (1-Methylethenyl) cyclohexene; p-mentha-l, 8-diene.

The following specific examples serve further to illustrate the practice and advantages of the invention.

Example 1. This describes a preliminary test to show the effectiveness of lignosulfonate for the intended purpose. In this and in Examples 2 to 5 the lignosulfonate was the primary "predisposing agent" to microbial colonization. It also served as a source of carbon for microbial growth. Indirectly, the mineral-complexing properties of lignosulfonate helped to release more mineral from the soil for microbial growth requirements. In Examples 1 to 5 it was the Georgia Pacific lignosulfonate described above.

Mixed stages of three nematode species were exposed 24 hours to various concentrations of lignosulfonate in shallow 50 ml beakers. Following exposure nematodes were inspected for activity level using touch response:

pp Concentration

Nematode 0 1000 2000 4000 8000 M. incognita 455/455 298/476 112/515 25/489 1/505 (J2 larvae)

P. penetrans 471/471 335/486 183/510 14/502 0/479 (mixed stages)

C. xenoplax 358/358 312/488 117/490 23/413 0/425 (mixed stages)

Counts are the number of active nematodes per total of 4 beakers per treatment. The figure preceding the slash mark is the count of surviving nematodes while the figure following the slash mark is the original number of nematodes.

Example 2. Potted Plant Tests.

A mixture of white, washed sand plus coarse river sand was mixed at a v/v ratio of 1:1. Rutgers tomato cuttings were started (1 each) in 6" clay pots using the sand mixture medium. Cuttings were allowed to develop for 1 month.

Kentucky Wonder bush beans were planted (1 each) in another set of 6" clay pots and allowed to develop for 1 month. A third set of pots were planted to rose cuttings (variety: Peace) and allowed to develop for 1.5 months. All plants were watered daily with 1/2 strength Hoagland's Solution. Tomato plants were inoculated with an aliquant of -2,000 J2 Meloidogyne incognita larvae per pot. Beans were inoculated with -2,000 mixed stage larvae of Pratylenehus penetrans per pot. Pots with rose plants were inoculated with ~2,000 mixed stage Criconemella xenoplax per pot. All nematodes were delivered in 20 ml of suspension with 4 ml placed into each of 5, 1" deep holes spaced evenly about the plant. Holes were covered with the sand mixture and lightly watered to seal air pockets. On the day following inoculation pots were treated with 4000 ppm (ca. 150 gpa) of calcium lignosulfonate solution (drenched to run-off) and again on the next day with 500 ml of the same solution. On the second treatment, pots were underlain with trays to catch run-off. All pots were left unwatered for an additional 24 hour before resuming watering with 1/2 strength Hoagland's Solution (with trays removed) . Nematodes and plants were allowed to grow for an additional 45 days before processing for gall and nematode counts. Controls were handled the same but treated with only 1/2 strength Hoagland's Solution. The initials M.i., P.p. and C.x. indicateMeloidogyne incognita. Pratylenehus penetrans and Criconemelle xenoplax. respectively. Similar in nomenclature is used throughout.

Replications

Treatment 1 2 3 4 5 Mean

M.i. Control 234 156 256 176 279 220

M.i. Treated 3 10 5 0 5 5

P.p. Control 3350 3530 4050 2980 3650 3512

P.p. Treated 13 7 2 19 2 9

C.x. Control 1580 1740 1380 1550 1900 1630

C.x. Treated 0 11 3 0 8 4 1. gph = gallons per acre

Counts for M. incognita represent galls; all other counts represent recovered nematodes. P. penetrans were recovered from both sand and roots, C. xenoplax from sand only.

Side Observation of Experiment 2 - Occasionally, bodies of nematodes were found heavily ramified with hyphae of fungi and/or dead with cleared internal structures oftentimes teeming with protozoa or bacteria. A casual examination of the soil microflora revealed predominately fungi of the genera Trichoderma and Penicillium.

Example 3. This example illustrates the role of soil microbiota in conjunction with the lignosulfonate. White sand and river sand were, again, used (1:1 v/v) and potted into 3" diameter clay pots. Half of the pots were autoclaved for 3 hours to sterilize the sand mixture. Half of the pots were left unsterilized. A portion of nematodes being used in the "sterile" treatments were surface sterilized by 2 hours exposure to a solution of 1000 ppm streptomycin sulfate plus 500 ppm cycloheximide. All surface sterilized nematodes were then washed with three repeated drenches of sterilized tap water before placing into agitated flasks for viability observations. Sterilized pots were kept semi-sterilized by placing them into sterilized "crispers" which minimized outside contamination. These sterilized pots were inoculated with 1000 surface sterilized nematodes (M. incognita, J2 stages; P. penetrans, mixed stages; C. xenoplax, mixed stages) . The unsterilized pots were inoculated with like numbers of nematodes not receiving surface sterilization treatments. All pots were wetted with sterilized tap water and the nematodes allowed to distribute in the sand mixture for 24 hours. Following 24 hours, half of the sterilized pots were drenched with 4000 ppm of a calcium lignosulfonate solution (250 ml; which had been sterilized by passage through coarse silicate filters and then 0.2 micron millipore filters) . All pots were underlain with sterilized dishes to catch run-off.

The other half of the sterilized pots were drenched with 250 ml of sterilized 1/2 strength Hoagland's Solution. Unsterilized pots were treated in a like manner but with unsterilized lignosulfonate solution (treated) and 1/2 strength Hoagland's Solution (control) . All pots were kept in crispers and periodically aerated (all pots maintained at ambient room temperature of 25° C) . Nematode counts, viability tests and/or degree of inactivity were made 30 days following treatment.

Replications

Treatment Mean

M.i. Sterile (C) 223 215 256 331 227 250

M.i. Sterile (T) 175 188 159 126 182 166

M.i. Nonster (C) 208 216 223 237 281 233

M.i. Nonster (T) 13 7 10 5 3 8

P.p. Sterile (C) 187 235 265 232 206 225

P.p. Sterile (T) 145 167 198 134 109 151

P.p. Nonster (C) 183 190 245 231 243 218

P.p. Nonster (T) 15 11 7 3 5 8

C.x. Sterile (C) 221 289 304 323 310 289

C.x. Sterile (T) 109 87 125 165 171 131

C.x. Nonster (C) 281 305 315 286 256 289

C.x. Nonster 2 4 10 7 7 6

NOTE: Treatments under non-sterilized conditions had significant mortality. It appears quite obvious that the initial weakening effect of the lignosulfonate solution in conjunction with the presence of invading microbes is primarily responsible for nematode mortality. C=Control, T=Treatment.

Although several of the nematodes survived the treatment under sterilized conditions, survivors looked sluggish with cleared intestinal tracts. Had soil microbiota been present and active, many of these would have succumbed completely to the treatment.

Example 4. This example compares the effects of different microbial species. It illustrates the fact that lignosulfonate with mixed species of fungi and soil bacteria provide better results than lignosulfonate alone or with fungi alone or with soil bacteria alone.

Mixed stages of C. xernoplax were surface sterilized and inoculated into sterilized pots, as outlined in Example 3. These pots were inoculated as follows:

(a) Sterilized lignosulfonate solution (4000 ppm) [TS] (b) Sterilized lignosulfonate solution +

(1) Mixed species of soil microbiota isolated from soil and grown on yeast extract nutrient agar (cultures grown for 7 days on 100 mm dia plates were washed with 250 ml of the lignosulfonate solution and this solution poured into treatment pots) ; Species identified included: [TM]

[Fungi: Penicillium sp., Trichoderma sp. , Mucor sp. , Cladosporium sp. ; -10 6th colony forming units/ml]

[Bacteria: Pseudomonas fluorescens, Arthrobacter sp. ; -10 8th cfu's/ml]

(2) Mixed species of soil fungi isolated from soil and grown on acidified potato dextrose agar: [TF]

[Fungi: Penicillium sp. , Trichoderma sp. , Mucor sp. , Cladosporium sp. ; -10 7th cfu's/ml]

(3) Mixed species of soil bacteria isolated from soil and grown on yeast extract nutrient agar: [TB]

[Bacteria: Pseudomonas fluorescens, Arthrobacter sp.; 10 8th cfu's/ml]

(c) Control treatment consisting of sterilized 1/2 strength Hoagland's Solution [CC]

(d) Control treatment consisting of sterilized 1/2 strength Hoagland's Solution + mixed species of

soil microbiota as in b-l, above; (similar concentration as in b-l above [CM]

Replications

Treatments 1 2 3 4 5 Mean

Control [CC] 325 301 296 340 335 319

Control [CM] 316 328 333 290 267 307

Treat [TS] 146 127 106 128 158 133

Treat [TM] 9 13 5 11 16 11

Treat [TF] 14 29 17 33 19 22

Treat fTBl 76 53 72 49 81 66

All pots were initially inoculated with -500 mixed stages of C. xenoplax.

Example 5. This example indicates the mechanism of lignosulfonate + mixed soil microbes (as in Example 4) .

Dead but intact nematodes from the above Example 4 which were treated with "Mixed Soil Microbes" were gently washed successively (5 repeated washings) and plated onto microbial media plates (yeast extract nutrient agar and acidified potato dextrose agar) . The predominately recovered microbes were fungi. Thus, it is believed that the weakened state of the nematode, initiated by lignosulfonate treatments, predisposes the nematode to initial attack by various species of fungi. Death and incipient degradation of the cadaver then opens avenues for invasion by several bacterial species. It appears as though the effects of the "total" soil microflora are more effective than any one group alone. Secondly, results from the "Treated, Unsterilized" trials in Example 3 suggest that additional microbes on the surface of nematodes may also contribute to mortality.

Example 6. Melon Study. Method.

A melon field had been treated with Treflan. The melon plants were young seedlings. There was visible damage from Treflan residue, the roots of seedlings typically bent near the soil line and swollen for 3" - 6" down towards the tap root. Secondly, nematode galls from diagnosed populations of Meloidogyne incognita (Root - Knot Nematode) were numerous averaging 16 galls per seedling (number of plants examined = 20) . The composition of Table 1 was shanked in at 30 gallons/acre with spades on each side of the seedling (approximately 8" on either side) at a depth of 3". Seedlings were irrigated via sprinklers to distribute the material into the root zone. One month following treatment plants were examined for top and root growth (shoot length and root girth) , galling and/or stunting and root swelling and bending. Root growth was evaluated on a 0 - 5 scale (5 = excellent) .

Results were as follows:

1. Top Growth

Replications

Treatment 1 _____ 3 4 5 Mean

Control 13" 15" 17" 14" 15" 15

Treated 38" 27" 39" 43" 45" 38

Note: Numbers reprresent length of lc >nges1t vine pe]r randou selected plant.

2. Nematode Galling

Replications

Treatment 1 2 3 _____ Mean

Control 25 32 27 41 32

Treated 18 13 15 19 16

Note: Numbers repress ≥nt q.ills per root (from nematode damage) . Average gall counts at the start of the experiment was 16.

3. Root Evaluation

Replications

Treatment _2_ 4_ Mean

Control 2 1 2 1 1 1.4

Treated 4 5 5 4 5 4.6

Note: Numbers represent root evaluation ratings with a 0-5 scale and 5 = excellent status.

Treflan, a product of Elanco Chemical Company, isTrifluralin (α,α,α-trifluro-2, 6-dinitro-N ,N-dipropyl-p- toluidine) 44.5% active ingredient applied at 2 pints/acre.

Table 1 referred to above, is as follows:

TABLE 1

Final concentration

% by wt of of active

% by wt component ingredient of active based on based on wt of

Component ingredient complete mix complete mix High Brix 50.0% Sugar 32.0% 16.0% Sugars

Cane or Beet Molasses

Calcium 50.0% CaLigno- 32.0% 16.0% CaLigno- Ligno- sulfonate sulfonate sulfonate

Urea 23.0% N 5.0% )

) 1.7% total

KN0 ₃ 13.9% N 3.8% ) Nitrogen KNO3 38.7% K 1.5% Potassium

H3PO4 23.7% P 3.4% 0.8% Phosphorus

ZnSO ₄ -7H ₂ 0 36.0% Zn 0.8% 0.3% Zinc

FeSO ₄ -7H ₂ 0 31.0% Fe 0.8% 0.3% Iron

MnSO ₄ -H ₂ 0 28.0% Mn 0.8% 0.2% Manganese Vitamin B 1.0% 0.04% B-Cplx Complex

Water (Tap) 20.4%

Example 7. In this example mites and aphids were the subjects of treatment and were present on the leaves of plants rather than in the soil. The effect was to stun the mites and aphids which dropped to the soil where, in normal field practice, they would be attacked by soil microbes.

The concentrate used (in diluted form) was that of

Table 2 below. In this Table the first column indicates the active ingredient, e.g., carbon skeleton-energy in the case of molasses; nitrogen, potassium and calcium in the case of calcium nitrate-potassium nitrate-urea mix, etc.

The second column indicates the form in which the active ingredient was added, e.g. , a mix of calcium nitrate, potassium nitrate and urea in the case of nitrogen; the third column indicates the percentage of active ingredient in each substance, e.g., 50% sugar in the case of molasses and 12% nitrogen in the case of calcium nitrate; the fourth column indicates the proportion of each source in the total concentrate, e.g., 67% molasses, and 3.4% phosphoric acid; and the last column indicates the percentage of active ingredient in the concentrate, e.g., 33.5% in the case of molasses and 1.10% in the case of nitrogen.

Calcium nitrate contributed nitrogen and calcium and potassium nitrate contributed potassium and nitrogen. The total nitrogen (contributed by calcium and potassium nitrates) and urea was 1.10%.

The term "CSE" or carbon skeleton-energy signifies a source of carbon skeleton and energy for plant metabolism.

TABLE 2

CSE Molasses 50.0% sugar 67.00% 33.50% High Brix

Nitrogen Ca(N0 ₃ )2-4H ₂ O 12.0% N See below

KN0 ₃ 13.9% N See below

Urea 23.0% N 1.16% 1.10%

Phosphorus Phosphoric acid 23.7% P 3.40% 0.80%

Potassium KOH 70.0% K 1.06% 1.50%

Calcium Ca(NOι)->-2H ₉ 0 17.0% Ca 4.69% 0.80%

Magnesium Mgso ₄ -H ₂ 0 9.9% Mg 1.50% 0.15%

Zinc ZnS0 ₄ 36.0% Zn 0.35% 0.13%

Iron FeS0 ₄ -2H ₂ 0 31.0% Fe 0.41% 0.13%

Manganese MnS0 ₄ -H ₂ 0 28.0% Mn 0.44% 0.13% Copper CuS0 ₄ -5H ₂ 0 25.2% Cu 0.08% 0.02%

Boron M3BO3 17.5% B 0.09% 0.02%

Molybdenum Na ₂ Mθ0 ₄ -H 0 39.7% Mo 0.002% 0.009%

Cobalt CθS0 ₄ -7H ₂ 0 20.8% Co 0.03% 0.01%

Vitamin-B Vitamin Premix 0.52% 0.02% Complex

Citric Acid C ₆ H ₈ 0 ₇ 99.9% Citrate 0.13% 0.01%

Ligno¬ CaSalt 50.0% Ca- Lig 7.00% 3.50% sulfonate

Water Nonchlorinated 10.20% 10.20%

Themites were the two spotted spidermite (Tetranychus urticae Koch) and the aphids were the peach aphid (Myzus persicae) . The hosts were, respectively, marigold and chrysanthum.

Naturally infested plants were sprayed with a 1:5 dilution of the composition of Table 2 and water. A household plant mister was used to deliver equal volumes of water and of this solution to foliage undersides of test plants (ca. 5 ml/plant) . A set of plants was left out as a complete control. Population counts were recorded 24 hours following treatment. Six leaves were randomly selected from top, middle and bottom portions of the plant (2 from each section) and infestation levels determined. In Series A, there was no treatment. In Series B, water was used. In Series C the above solution was used.

RESULTS

HITE POPULATIONS: Replications Treatment 1 10 Mean

Series A 123 115 142 102 89 95 113 93 109 75 a 106

Series B 73 97 105 84 90 72 63 88 69 57 •b 80

Series C 2 7 7 3 5 1 0 2 5 2 *c 3

Figures represent total numbers of two-spotted mites (all stages except eggs) per 6 fandomly selected leaves. * = significantly different at the 5% level.

APHID POPULATIONS: Repl ications

Treatment 1 2 3 4 5 6 7 8 9 10 Mean

Coπ-pl Ck 21 17 37 19 23 23 16 22 18 25 a 22

Ck 16 24 26 18 31 24 17 19 20 23 a 22

BrSn 0 0 0 1 0 0 0 0 0 0 *b 0

Figures represent total numbers of peach aphids per 6 randomly selected leaves. * = significantly different at the 5% level.

Following is a discussion of ingredients of the soil treatment composition and of concentrations and rates of application.

As stated above the preferred pre-disposition substance is a water soluble lignosulfonate but other substances such as those listed above may be used. Also a mixture of any two or more of such substances may be used.

As stated above the predisposing substance together with other substances such as nutrients may be applied to soil in aqueous solution by sprinkling, by drip methods, or by injection in the soil followed by irrigation or by any other suitable method. It may also be applied in dry form and then infiltrated into the soil by irrigation.

Solutions may be applied in which the predisposing substance, e.g., lignosulfonate is present in concentrations of 10 or less to 50% or more by weight with other ingredients present in the proportions indicated by rates of application as discussed below.

Rates of application may be as follows in parts per million per acre foot of soil, although greater or lesser amounts may be used provided the predisposing substance is

applied in amount sufficient to accomplish the intended result.

Predisposing Substance 50-1500

Nitrogen 10-150 Phosphorus 10-150

Vitamin/co-factor 5-100

Microbes- 2-50 grams per acre foot

In addition potassium, zinc, manganese and iron may be included in amounts as follows in parts per acre foot of soil:

Potassium 10-150

Zinc 0.5-50

Manganese 0.5-50

Iron 0.5-50

In Table 1 above, molasses was used as a major component. It provides a nutrient source for soil microbes.

Substitutes for molasses include various sugars, e.g. , mannose, dextrose, lactose, cornsteed liquor, corn syrup and whey. Suitable sources of nitrogen include urea, ammonium nitrate, calcium nitrate, potassium nitrate, ammonium sulfate, aqua ammonia, URAN, proteins, amino acids, and mixtures thereof.

Suitable sources of phosphorus include phosphoric acid, potassium phosphate, potassium pyrophosphate, ammonium phosphate nitrate, ammoniated triple superphosphate, ammoniated single superphosphate, ammonium phosphate, and mixtures thereof.

Suitable sources of potassium are potassium chloride, potassium sulfate, potassium gluconate, potassium acetate and potassium citrate, and mixtures thereof.

Suitable sources of zinc are zinc acetate, zinc sulfate, zinc benzoate, zinc chloride and zinc citrate, and mixtures thereof.

Suitable sources of manganese are manganese acetate, manganese chloride and manganese nitrate, and mixtures thereof.

Suitable sources of iron are ferric chloride, ferric sulfate, ferric citrate and ferric nitrate, and mixtures thereof.

It will be understood that a single substance, e.g., potassium phosphate, may provide a source of more than one nutrient. Suitable vitamin/cofactor sources are yeast extract, thiamine pyrophosphate, riboflavin acetyl phosphate, biotin sulfoxide, pantothenicacid, phosphatidylcholine, inositol, PABA, nicotinic acid, folic acid, and mixtures thereof.

Suitable microbes include (1) fungi such as Trichoderma viride, T. hamatum, T. harzianum, T. koningii, Penicillium oxalicum, P. funiculosum, P. urticae, P. vermiculatum, Gliocladium roseum, G. virens, Chaetomium globosum, Dactylella oviparasitica, Verticillium lacanii, Fusarium moniliforme var subglutinans, Arthrobotrys amerospora, A. conoides, Acremonium boreale, A. falciforme, coniothyrium initans, and mixtures thereof and (2) bacteria such as Pseudomonas fluorescens, Ps. putida, Enterobacter cloacae, Alcaligines spp., Arthrobacter citreus, A. globiformis, A. crystallopoietes. Bacillus subtilis, B. cereus, B. pumilus, B. mycoides, B. megaterium, Streptomyces griseus, S. aureofaciens, and mixtures thereof.

It will, therefore, be apparent that a new and useful method of nematode control has been provided.