Title:
CORNEAL ENDOTHELIAL CELL MARKER
Document Type and Number:
WIPO Patent Application WO/2015/016371
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method of purification and preparation of cultured corneal endothelial cells, and in particular, to provide cell surface markers for use in corneal endothelial cells not including transformed cells. Provided are cell markers for distinguishing normal cells and transformed cells, in particular normal and transformed corneal endothelium cells. These cell markers relate to specific cell surface markers, for example, to a normal corneal endothelial surface marker such as CD166, and a transformed cell surface marker such as CD73. By using the transformed cell surface marker such as CD73 to remove transformed cells by sorting, it becomes possible to improve purity of a normal cultured corneal endothelium. By using normal corneal endothelial surface marker such as CD166, or by combined use with the transformed cell surface marker, it becomes possible to provide a means for verifying the purity of a prepared corneal endothelium.
Inventors:
KOIZUMI NORIKO (JP)
OKUMURA NAOKI (JP)
HIRANO HIROATSU (JP)
KINOSHITA SHIGERU (JP)
UENO MORIO (JP)
OKUMURA NAOKI (JP)
HIRANO HIROATSU (JP)
KINOSHITA SHIGERU (JP)
UENO MORIO (JP)
Application Number:
PCT/JP2014/070412
Publication Date:
February 05, 2015
Filing Date:
July 28, 2014
Export Citation:
Assignee:
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
DOSHISHA (JP)
SENJU PHARMA CO (JP)
DOSHISHA (JP)
SENJU PHARMA CO (JP)
International Classes:
C12N15/09; A61K35/12; A61L27/00; A61P27/02; C12N5/071; C12N5/10; C12Q1/02; C12Q1/68; G01N33/53
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| WO2013100208A1 | 2013-07-04 | |||
| WO2013086236A2 | 2013-06-13 | |||
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YU MATSUZAKI ET AL.: "Kakumaku Naihi Saibo Hyomen Marker no Kento", REGENERATIVE MEDICINE, vol. 10, 2011, pages 201
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SAISEI IRYO, REGENERATIVEMEDICINE, vol. 9, 5 February 2010 (2010-02-05), pages 283
THE PROCEEDINGS OF THE GENERAL MEETING OF THE JAPAN CORNEA SOCIETY/KERATOPLASTY SOCIETY OF JAPAN PROGRAM, vol. 35TH-27T, 2011, pages 75
AM J PATHOL., vol. 178, no. 2, February 2011 (2011-02-01), pages 572 - 579
CYTOTHERAPY, vol. 9, no. 3, 2007, pages 252 - 258
"Japanese Journal of Ophthalmology", vol. 117, 4 March 2013, pages: 378
KUNZEVITZKY, NJ ET AL., ARVO E, 2013
MEHTA JS ET AL., ARVO E, 2013
SAISEI IRYO: "Regenerative Medicine", vol. 10, 1 February 2011, pages: 201
"Japanese Journal of Ophthalmology", vol. 105, 15 March 2001, pages: 195
BATZER ET AL., NUCLEIC ACID RES., vol. 19, 1991, pages 5081
OHTSUKA ET AL., J.BIOL.CHEM., vol. 260, 1985, pages 2605 - 2608
ROSSOLINI ET AL., MOL. CELL. PROBES, vol. 8, 1994, pages 91 - 98
NATURE, vol. 409, 2001, pages 860 - 921
"DNA Cloning 1: Core Techniques, A Practical Approach", 1995, OXFORD UNIVERSITY PRESS, article "Molecular Cloning"
ALTSCHUL ET AL., J.MOL.BIOL., vol. 215, 1990, pages 403 - 410
PEARSON; LIPMAN, PROC.NATL.ACAD.SCI., vol. 85, 1988, pages 2444 - 2448
SMITH; WATERMAN, J.MOL.BIOL., vol. 147, 1981, pages 195 - 197
NEEDLEMAN; WUNSCH, J.MOL.BIOL., vol. 48, 1970, pages 443 - 453
MATSUBARA M; TANISHIMA T: "Wound-healing of the corneal endothelium in the monkey: a morphometric study", JPN J OPHTHALMOL, vol. 26, 1982, pages 264 - 273
MATSUBARA M; TANISHIMA T: "Wound-healing of corneal endothelium in monkey: an autoradiographic study", JPN J OPHTHALMOL, vol. 27, 1983, pages 444 - 450
VAN HORN DL; HYNDIUK RA: "Endothelial wound repair in primate cornea", EXP EYE RES, vol. 21, 1975, pages 113 - 124, XP026214808, DOI: doi:10.1016/0014-4835(75)90076-7
VAN HORN DL; SENDELE DD; SEIDEMAN S; BUCO PJ: "Regenerative capacity of the corneal endothelium in rabbit and cat", INVEST OPHTHALMOL VIS SCI, vol. 16, 1977, pages 597 - 613
LEUNG, T. E, J. BIOL. CHEM., vol. 270, 1995, pages 29051 - 29054
ISHIZAKI, T. ET AL., EMBO J., vol. 15, no. 8, 1996, pages 1885 - 1893
NAT GENET., vol. 32, December 2002 (2002-12-01), pages 526 - 532
"Nakamura Yusuke Lab. Manual", 2002, article "Genome Analysis Experimental Method"
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KOIZUMI N; OKUMURA N; KINOSHITA S., EXPERIMENTAL EYE RESEARCH, vol. 95, 2012, pages 60 - 67
UENO M; MATSUMURA M; WATANABE K; NAKAMURA T; OSAKADA F; TAKAHASHI M; KAWASAKI H; KINOSHITA S; SASAI Y, PROC NATL ACAD SCI USA., vol. 103, no. 25, 2006, pages 9554 - 9559
"DNA Cloning 1: Core Techniques, A Practical Approach", 1995
SAMBROOK, J: "Molecular Cloning", pages: 9.47 - 9.58
"Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS, pages: 9.47 - 9.58
SAMBROOK J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR
"Current Protocols in Molecular Biology", 1987, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
AUSUBEL, F.M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1989, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
INNIS, M.A: "PCR Protocols: A Guide to Methods and Applications", 1990, ACADEMIC PRESS
AUSUBEL, F. M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1992, GREENE PUB. ASSOCIATES
AUSUBEL, F.M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1995, GREENE PUB. ASSOCIATES
INNIS, M.A. ET AL.: "PCR Strategies", 1995, ACADEMIC PRESS
AUSUBEL, F.M.: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1999, WILEY
SNINSKY, J.J. ET AL.: "PCR Applications: Protocols for Functional Genomics", 1999, ACADEMIC PRESS
"Gene Introduction & Expression Analysis Experimental Method", 1997, YODOSHA CO., LTD.
GAIT, M. J.: "Oligonucleotide Synthesis: A Practical Approach", 1985, IRL PRESS
GAIT, M. J.: "Oligonucleotide Synthesis: A Practical Approach", 1990, IRL PRESS
ECKSTEIN, F.: "Oligonucleotides and Analogues: A Practical Approach", 1991, IRL PRESS
ADAMS, R. L. ET AL.: "The Biochemistry of the Nucleic Acids", 1992, CHAPMAN & HALL
SHABAROVA, Z ET AL.: "Advanced Organic Chemistry of Nucleic Acids", 1994, WEINHEIM
BLACKBURN, G. M. ET AL.: "Nucleic Acids in Chemistry and Biology", 1996, OXFORD UNIVERSITY PRESS
HERMANSON, G. T.: "Bioconjugate Techniques", 1996, ACADEMIC PRESS
STEIN ET AL., NUCL. ACIDS RES., vol. 16, 1988, pages 3209
SARIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 7448 - 7451
KOIZUMI N ET AL., EXP EYE RES., vol. 95, 2012, pages 60 - 67
KOIZUMI N ET AL., INVEST OPHTHALMOL VIS SCI., vol. 48, 2007, pages 4519 - 4526
OKUMURA N ET AL., AM J PATHOL, vol. 181, 2012, pages 268 - 277
YU MATSUZAKI ET AL.: "Kakumaku Naihi Saibo Hyomen Marker no Kento", REGENERATIVE MEDICINE, vol. 10, 2011, pages 201
See also references of EP 3029140A4
SAISEI IRYO, REGENERATIVEMEDICINE, vol. 9, 5 February 2010 (2010-02-05), pages 283
THE PROCEEDINGS OF THE GENERAL MEETING OF THE JAPAN CORNEA SOCIETY/KERATOPLASTY SOCIETY OF JAPAN PROGRAM, vol. 35TH-27T, 2011, pages 75
AM J PATHOL., vol. 178, no. 2, February 2011 (2011-02-01), pages 572 - 579
CYTOTHERAPY, vol. 9, no. 3, 2007, pages 252 - 258
"Japanese Journal of Ophthalmology", vol. 117, 4 March 2013, pages: 378
KUNZEVITZKY, NJ ET AL., ARVO E, 2013
MEHTA JS ET AL., ARVO E, 2013
SAISEI IRYO: "Regenerative Medicine", vol. 10, 1 February 2011, pages: 201
"Japanese Journal of Ophthalmology", vol. 105, 15 March 2001, pages: 195
BATZER ET AL., NUCLEIC ACID RES., vol. 19, 1991, pages 5081
OHTSUKA ET AL., J.BIOL.CHEM., vol. 260, 1985, pages 2605 - 2608
ROSSOLINI ET AL., MOL. CELL. PROBES, vol. 8, 1994, pages 91 - 98
NATURE, vol. 409, 2001, pages 860 - 921
"DNA Cloning 1: Core Techniques, A Practical Approach", 1995, OXFORD UNIVERSITY PRESS, article "Molecular Cloning"
ALTSCHUL ET AL., J.MOL.BIOL., vol. 215, 1990, pages 403 - 410
PEARSON; LIPMAN, PROC.NATL.ACAD.SCI., vol. 85, 1988, pages 2444 - 2448
SMITH; WATERMAN, J.MOL.BIOL., vol. 147, 1981, pages 195 - 197
NEEDLEMAN; WUNSCH, J.MOL.BIOL., vol. 48, 1970, pages 443 - 453
MATSUBARA M; TANISHIMA T: "Wound-healing of the corneal endothelium in the monkey: a morphometric study", JPN J OPHTHALMOL, vol. 26, 1982, pages 264 - 273
MATSUBARA M; TANISHIMA T: "Wound-healing of corneal endothelium in monkey: an autoradiographic study", JPN J OPHTHALMOL, vol. 27, 1983, pages 444 - 450
VAN HORN DL; HYNDIUK RA: "Endothelial wound repair in primate cornea", EXP EYE RES, vol. 21, 1975, pages 113 - 124, XP026214808, DOI: doi:10.1016/0014-4835(75)90076-7
VAN HORN DL; SENDELE DD; SEIDEMAN S; BUCO PJ: "Regenerative capacity of the corneal endothelium in rabbit and cat", INVEST OPHTHALMOL VIS SCI, vol. 16, 1977, pages 597 - 613
LEUNG, T. E, J. BIOL. CHEM., vol. 270, 1995, pages 29051 - 29054
ISHIZAKI, T. ET AL., EMBO J., vol. 15, no. 8, 1996, pages 1885 - 1893
NAT GENET., vol. 32, December 2002 (2002-12-01), pages 526 - 532
"Nakamura Yusuke Lab. Manual", 2002, article "Genome Analysis Experimental Method"
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KOIZUMI N; OKUMURA N; KINOSHITA S., EXPERIMENTAL EYE RESEARCH, vol. 95, 2012, pages 60 - 67
UENO M; MATSUMURA M; WATANABE K; NAKAMURA T; OSAKADA F; TAKAHASHI M; KAWASAKI H; KINOSHITA S; SASAI Y, PROC NATL ACAD SCI USA., vol. 103, no. 25, 2006, pages 9554 - 9559
"DNA Cloning 1: Core Techniques, A Practical Approach", 1995
SAMBROOK, J: "Molecular Cloning", pages: 9.47 - 9.58
"Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS, pages: 9.47 - 9.58
SAMBROOK J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR
"Current Protocols in Molecular Biology", 1987, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
AUSUBEL, F.M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1989, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
INNIS, M.A: "PCR Protocols: A Guide to Methods and Applications", 1990, ACADEMIC PRESS
AUSUBEL, F. M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1992, GREENE PUB. ASSOCIATES
AUSUBEL, F.M: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1995, GREENE PUB. ASSOCIATES
INNIS, M.A. ET AL.: "PCR Strategies", 1995, ACADEMIC PRESS
AUSUBEL, F.M.: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", 1999, WILEY
SNINSKY, J.J. ET AL.: "PCR Applications: Protocols for Functional Genomics", 1999, ACADEMIC PRESS
"Gene Introduction & Expression Analysis Experimental Method", 1997, YODOSHA CO., LTD.
GAIT, M. J.: "Oligonucleotide Synthesis: A Practical Approach", 1985, IRL PRESS
GAIT, M. J.: "Oligonucleotide Synthesis: A Practical Approach", 1990, IRL PRESS
ECKSTEIN, F.: "Oligonucleotides and Analogues: A Practical Approach", 1991, IRL PRESS
ADAMS, R. L. ET AL.: "The Biochemistry of the Nucleic Acids", 1992, CHAPMAN & HALL
SHABAROVA, Z ET AL.: "Advanced Organic Chemistry of Nucleic Acids", 1994, WEINHEIM
BLACKBURN, G. M. ET AL.: "Nucleic Acids in Chemistry and Biology", 1996, OXFORD UNIVERSITY PRESS
HERMANSON, G. T.: "Bioconjugate Techniques", 1996, ACADEMIC PRESS
STEIN ET AL., NUCL. ACIDS RES., vol. 16, 1988, pages 3209
SARIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 7448 - 7451
KOIZUMI N ET AL., EXP EYE RES., vol. 95, 2012, pages 60 - 67
KOIZUMI N ET AL., INVEST OPHTHALMOL VIS SCI., vol. 48, 2007, pages 4519 - 4526
OKUMURA N ET AL., AM J PATHOL, vol. 181, 2012, pages 268 - 277
Attorney, Agent or Firm:
YAMAMOTO, Shusaku et al. (JP)
Shusaku Yamamoto (JP)
Shusaku Yamamoto (JP)
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