Title:
CORONAVIRUS RBD PROTEIN-FLUORESCENT MICROSPHERE COMPLEX PREPARATION
Document Type and Number:
WIPO Patent Application WO/2023/142398
Kind Code:
A1
Abstract:
Disclosed is a coronavirus RBD protein-fluorescent microsphere complex preparation. The complex preparation comprises raw materials in the following percentages by mass: 0.5%-1% of a conjugate protein, 2.5%-5% of saccharide, 0.9%-1.3% of ions, 0.05%-0.07% of macromolecular substances, 0.05%-0.1% of a surfactant, 0.24-1.21% of a buffer and 0.02-0.05% of a preservative. The complex preparation can be used for the preservation, dilution and treatment of coronavirus RBD protein fluorescent complexes and biological samples. The biological samples comprise serum and plasma. When neutralizing antibodies in human serum, plasma and whole-blood samples are detected by means of a saturation competition method, the time-resolved fluorescent microsphere complex coupled with a coronavirus RBD protein need to be stored separately. The complex preparation can prevent the fluorescent microsphere complex from aggregation and precipitation, maintains the antigenic activity and improves the detection sensitivity.
Inventors:
WANG LIJIAO (CN)
PENG JIE (CN)
ZHANG BIANQIANG (CN)
YU DI (CN)
SHENG CHANGZHONG (CN)
SU YAN (CN)
ZHOU ZEQI (CN)
PENG JIE (CN)
ZHANG BIANQIANG (CN)
YU DI (CN)
SHENG CHANGZHONG (CN)
SU YAN (CN)
ZHOU ZEQI (CN)
Application Number:
PCT/CN2022/107066
Publication Date:
August 03, 2023
Filing Date:
July 21, 2022
Export Citation:
Assignee:
DYNAMIKER BIOTECHNOLOGY TIANJIN CO LTD (CN)
International Classes:
G01N33/533
Foreign References:
| CN114113579A | 2022-03-01 | |||
| CN113834943A | 2021-12-24 | |||
| CN112946260A | 2021-06-11 | |||
| CN111239419A | 2020-06-05 | |||
| CN113156131A | 2021-07-23 | |||
| US20200124625A1 | 2020-04-23 | |||
| CN111551713A | 2020-08-18 | |||
| CN111562369A | 2020-08-21 | |||
| CN111896734A | 2020-11-06 |
Attorney, Agent or Firm:
TIANJIN ZHIYUAN JUNZHENG PATENT AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
Download PDF: