Title:
CORONAVIRUS VACCINE COMPOSITION, METHODS, AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/142786
Kind Code:
A1
Abstract:
Provided is an immunogenic composition comprising a recombinant peptide and protein. The recombinant peptide and protein comprise a coronavirus antigen and immunogen, for example, an S protein peptide of a SARS-CoV-2 coronavirus beta (B.1.351) variant or a fragment, a variant or a mutant thereof, such as a chimeric antigen and immunogen comprising a receptor-binding domain of the beta variant and an S protein peptide sequence of an original strain or other variants. The immunogenic composition comprises a secreted fusion protein, the secreted fusion protein comprising a soluble coronavirus antigen. The soluble coronavirus antigen protein is connected, by means of an in-frame fusion, to a C-terminal moiety of a collagen capable of self-trimerization to form a disulfide bond-linked trimeric fusion protein. The provided immunogenic composition can be used for generating an immune response, for example, used as a vaccine for the prevention of coronavirus infections, such as infections by SARS-CoV-2 original (Hu-1), alpha, beta, gamma, delta, omicron strains and/or other strains. The provided immunogenic composition can be used in a vaccine composition, for example, as part of a prophylactic and/or therapeutic vaccine. Also provided are methods for producing the recombinant peptide and protein, methods for the prevention, treatment and/or diagnosis, and a related kit.
Inventors:
LIANG PENG (CN)
LIANG JOSHUA (CN)
SU DANMEI (CN)
LIANG JOSHUA (CN)
SU DANMEI (CN)
Application Number:
PCT/CN2022/140514
Publication Date:
August 03, 2023
Filing Date:
December 21, 2022
Export Citation:
Assignee:
SICHUAN CLOVER BIOPHARMACEUTICALS INC (CN)
International Classes:
A61K38/00; C07K19/00; A61P31/00; C07K14/00
Domestic Patent References:
| WO2021249451A1 | 2021-12-16 | |||
| WO2021249012A1 | 2021-12-16 |
Other References:
DATABASE Protein 11 May 2021 (2021-05-11), ANONYMOUS : "surface glycoprotein [synthetic construct] ", XP093081901, retrieved from NCBI Database accession no. QUT64557.
WANG HONGYE, WANG ZENGSHUAI, MA LIANG, ZHU XIAOYONG, LI BINGXIANG, HUANG YUHANG, LI JINGWEN, SUN MING, SHI LI, YAO YUFENG: "S Trimer Derived from SARS-CoV-2 B.1.351 and B.1.618 Induced Effective Immune Response against Multiple SARS-CoV-2 Variants", VACCINES, vol. 11, no. 1, pages 193, XP093081904, DOI: 10.3390/vaccines11010193
LIANG JOSHUA G., SU DANMEI, SONG TIAN-ZHANG, ZENG YILAN, HUANG WEIJIN, WU JINHUA, XU RONG, LUO PEIWEN, YANG XIAOFANG, ZHANG XIAODO: "S-Trimer, a COVID-19 subunit vaccine candidate, induces protective immunity in nonhuman primates", NATURE COMMUNICATIONS, vol. 12, no. 1, 1 December 2021 (2021-12-01), XP055873177, DOI: 10.1038/s41467-021-21634-1
YANG LIMIN, TIAN DEYU, HAN JIAN-BAO, FAN WENHUI, ZHANG YUAN, LI YUNLONG, SUN WENQIANG, WEI YANQIU, TIAN XIAODONG, YU DAN-DAN, FENG: "A recombinant receptor-binding domain in trimeric form generates protective immunity against SARS-CoV-2 infection in nonhuman primates", THE INNOVATION, vol. 2, no. 3, 1 August 2021 (2021-08-01), pages 100140, XP093081911, ISSN: 2666-6758, DOI: 10.1016/j.xinn.2021.100140
MOREAU YANNIS, GIL PATRICIA, EXBRAYAT ANTONI, RAKOTOARIVONY IGNACE, BRÉARD EMMANUEL, SAILLEAU CORINNE C., VIAROUGE CYRIL, ZIENTARA: "The Genome Segments of Bluetongue Virus Differ in Copy Number in a Host-Specific Manner", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 95, no. 1, 1 January 2021 (2021-01-01), US , pages 1 - 14, XP093081913, ISSN: 0022-538X, DOI: 10.1128/JVI
YANG YONG, ZANG JINKAI, XU SHIQI, ZHANG XUEYANG, YUAN SULE, WANG HAIKUN, LAVILLETTE DIMITRI, ZHANG CHAO, HUANG ZHONG: "Elicitation of Broadly Neutralizing Antibodies against B.1.1.7, B.1.351, and B.1.617.1 SARS-CoV-2 Variants by Three Prototype Strain-Derived Recombinant Protein Vaccines", VIRUSES, vol. 13, no. 8, pages 1421, XP093081917, DOI: 10.3390/v13081421
LIU YUAN, DAI LIANPAN, FENG XIAOLI, GAO RAN, ZHANG NAN, WANG BIN, HAN JIANBAO, ZOU QINGCUI, GUO XILING, ZHU HUA, LIU JIANGNING, QI: "Fast and long-lasting immune response to S-trimer COVID-19 vaccine adjuvanted by PIKA", MOLECULAR BIOMEDICINE, vol. 2, no. 1, 1 December 2021 (2021-12-01), XP093081919, DOI: 10.1186/s43556-021-00054-z
RICHMOND PETER C, HATCHUEL LARA, PACCIARINI FILIPPO, HU BRANDA, SMOLENOV IGOR, LI PING, LIANG PENG, HAN HTAY HTAY, LIANG JOSHUA, C: "Persistence of the Immune Responses and Cross-Neutralizing Activity With Variants of Concern Following 2 Doses of Adjuvanted SCB-2019 Coronavirus Disease 2019 Vaccine", JOURNAL OF INFECTIOUS DISEASES, UNIVERSITY OF CHICAGO PRESS, US, 4 September 2021 (2021-09-04), US , XP093081927, ISSN: 0022-1899, DOI: 10.1093/infdis/jiab447
WANG HONGYE, WANG ZENGSHUAI, MA LIANG, ZHU XIAOYONG, LI BINGXIANG, HUANG YUHANG, LI JINGWEN, SUN MING, SHI LI, YAO YUFENG: "S Trimer Derived from SARS-CoV-2 B.1.351 and B.1.618 Induced Effective Immune Response against Multiple SARS-CoV-2 Variants", VACCINES, vol. 11, no. 1, pages 193, XP093081904, DOI: 10.3390/vaccines11010193
LIANG JOSHUA G., SU DANMEI, SONG TIAN-ZHANG, ZENG YILAN, HUANG WEIJIN, WU JINHUA, XU RONG, LUO PEIWEN, YANG XIAOFANG, ZHANG XIAODO: "S-Trimer, a COVID-19 subunit vaccine candidate, induces protective immunity in nonhuman primates", NATURE COMMUNICATIONS, vol. 12, no. 1, 1 December 2021 (2021-12-01), XP055873177, DOI: 10.1038/s41467-021-21634-1
YANG LIMIN, TIAN DEYU, HAN JIAN-BAO, FAN WENHUI, ZHANG YUAN, LI YUNLONG, SUN WENQIANG, WEI YANQIU, TIAN XIAODONG, YU DAN-DAN, FENG: "A recombinant receptor-binding domain in trimeric form generates protective immunity against SARS-CoV-2 infection in nonhuman primates", THE INNOVATION, vol. 2, no. 3, 1 August 2021 (2021-08-01), pages 100140, XP093081911, ISSN: 2666-6758, DOI: 10.1016/j.xinn.2021.100140
MOREAU YANNIS, GIL PATRICIA, EXBRAYAT ANTONI, RAKOTOARIVONY IGNACE, BRÉARD EMMANUEL, SAILLEAU CORINNE C., VIAROUGE CYRIL, ZIENTARA: "The Genome Segments of Bluetongue Virus Differ in Copy Number in a Host-Specific Manner", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 95, no. 1, 1 January 2021 (2021-01-01), US , pages 1 - 14, XP093081913, ISSN: 0022-538X, DOI: 10.1128/JVI
YANG YONG, ZANG JINKAI, XU SHIQI, ZHANG XUEYANG, YUAN SULE, WANG HAIKUN, LAVILLETTE DIMITRI, ZHANG CHAO, HUANG ZHONG: "Elicitation of Broadly Neutralizing Antibodies against B.1.1.7, B.1.351, and B.1.617.1 SARS-CoV-2 Variants by Three Prototype Strain-Derived Recombinant Protein Vaccines", VIRUSES, vol. 13, no. 8, pages 1421, XP093081917, DOI: 10.3390/v13081421
LIU YUAN, DAI LIANPAN, FENG XIAOLI, GAO RAN, ZHANG NAN, WANG BIN, HAN JIANBAO, ZOU QINGCUI, GUO XILING, ZHU HUA, LIU JIANGNING, QI: "Fast and long-lasting immune response to S-trimer COVID-19 vaccine adjuvanted by PIKA", MOLECULAR BIOMEDICINE, vol. 2, no. 1, 1 December 2021 (2021-12-01), XP093081919, DOI: 10.1186/s43556-021-00054-z
RICHMOND PETER C, HATCHUEL LARA, PACCIARINI FILIPPO, HU BRANDA, SMOLENOV IGOR, LI PING, LIANG PENG, HAN HTAY HTAY, LIANG JOSHUA, C: "Persistence of the Immune Responses and Cross-Neutralizing Activity With Variants of Concern Following 2 Doses of Adjuvanted SCB-2019 Coronavirus Disease 2019 Vaccine", JOURNAL OF INFECTIOUS DISEASES, UNIVERSITY OF CHICAGO PRESS, US, 4 September 2021 (2021-09-04), US , XP093081927, ISSN: 0022-1899, DOI: 10.1093/infdis/jiab447
Download PDF:
Previous Patent: ANTENNA ASSEMBLY AND ELECTRONIC DEVICE
Next Patent: POWER AMPLIFIER (PA) FAILURE ISOLATION METHOD, TERMINAL DEVICE AND STORAGE MEDIUM
Next Patent: POWER AMPLIFIER (PA) FAILURE ISOLATION METHOD, TERMINAL DEVICE AND STORAGE MEDIUM