Title:
CORYNEBACTERIUM ATCC 13032 IMPROVED STRAIN CAPABLE OF EFFECTIVELY EXPRESSING FOREIGN PROTEIN AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/103498
Kind Code:
A1
Abstract:
A corynebacterium ATCC 13032 improved strain capable of effectively expressing a foreign protein and a construction method therefor. The improved strain is a strain obtained by knocking out cglIM genes and a prophage genome in corynebacterium glutamicum ATCC 13032 on the basis of the corynebacterium glutamicum ATCC 13032. The improved strain can effectively express a foreign protein by knocking out prophage genes and knocking out genes of a restriction enzyme modification system.
Inventors:
CHEN RUIAI (CN)
HUANG MEI (CN)
LIU DINGXIANG (CN)
LI YANPENG (CN)
YAN YUANYUAN (CN)
DONG NAN (CN)
YANG XIAOYUN (CN)
HUANG MEI (CN)
LIU DINGXIANG (CN)
LI YANPENG (CN)
YAN YUANYUAN (CN)
DONG NAN (CN)
YANG XIAOYUN (CN)
Application Number:
PCT/CN2020/096712
Publication Date:
June 03, 2021
Filing Date:
June 18, 2020
Export Citation:
Assignee:
ZHAOQING INST OF BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N1/21; C12N15/77; C12P1/00
Domestic Patent References:
| WO2002024917A2 | 2002-03-28 |
Foreign References:
| CN111073841A | 2020-04-28 | |||
| CN108611311A | 2018-10-02 | |||
| CN106190921A | 2016-12-07 | |||
| CN103409446A | 2013-11-27 |
Other References:
BAUMGART MEIKE, UNTHAN SIMON, RÜCKERT CHRISTIAN, SIVALINGAM JASINTHA, GRÜNBERGER ALEXANDER, KALINOWSKI JÖRN, BOTT MICHAEL, NOACK S: "Construction of a Prophage-Free Variant of Corynebacterium glutamicum ATCC 13032 for Use as a Platform Strain for Basic Research and Industrial Biotechnology", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 79, no. 19, 1 October 2013 (2013-10-01), US, pages 6006 - 6015, XP055815382, ISSN: 0099-2240, DOI: 10.1128/AEM.01634-13
SCHAEFER A, ET AL.: "THE CORYNEBACTERIUM GLUTAMICUM CGLIM GENE ENCODING A 5-CYTOSINE METHYLTRANSFERASE ENZYME CONFERS A SPECIFIC DNA METHYLATION PATTERN IN AN MCRBC-DEFICIENT ESCHERICHIA COLI STRAIN", GENE., ELSEVIER, AMSTERDAM., NL, vol. 203, no. 02, 12 December 1997 (1997-12-12), NL, pages 95 - 101, XP001056557, ISSN: 0378-1119, DOI: 10.1016/S0378-1119(97)00519-2
WANG LEI, WANG JUNQING, XUE LE, LI PIWU, WANG TENGFEI, SU JING, WANG RUIMING: "Construction and Application of a Vector for Marker-free Targeted Genetic Knockout in Corynebacterium Glutamicum", CHINA BREWING, ZHONGGUO NIANGZAO BIANJIBU, CN, vol. 37, no. 2, 1 January 2018 (2018-01-01), CN, pages 121 - 126, XP055815544, ISSN: 0254-5071, DOI: 10.11882/j.issn.0254-5071.2018.02.025
YU YING, XU MEI-XUE, LIU JIN-LEI, FAN RONG, FENG HUI-YONG, LI TIAN-MING: "Metabolic Engineering for Modifying Corynebacterium glutamicum to Produce More Pyruvate", BIOTECHNOLOGY BULLETIN, vol. 32, no. 8, 1 January 2016 (2016-01-01), pages 226 - 232, XP055815546, DOI: 10.13560/j.cnki.biotech.bull.1985.2016.08.033
LI TIEMIN, HU YONG-FEI, FENG QIAN-NI, LI YU, DONG ZHAN-SHUANG, LIU ZHONG-SHUN, LUO EN-JIE: "Functional Activity Analysis of CglI Gene in E.coli Strain", WEISHENGWUXUE ZAZHI - ACTA MICROBIOLOGICA SINICA, LIAONING SHENG WEISHENGWU YANJIUSUO, CN, vol. 26, no. 2, 1 March 2006 (2006-03-01), CN, pages 41 - 44, XP055815549, ISSN: 1005-7021
SCHAEFER A, ET AL.: "THE CORYNEBACTERIUM GLUTAMICUM CGLIM GENE ENCODING A 5-CYTOSINE METHYLTRANSFERASE ENZYME CONFERS A SPECIFIC DNA METHYLATION PATTERN IN AN MCRBC-DEFICIENT ESCHERICHIA COLI STRAIN", GENE., ELSEVIER, AMSTERDAM., NL, vol. 203, no. 02, 12 December 1997 (1997-12-12), NL, pages 95 - 101, XP001056557, ISSN: 0378-1119, DOI: 10.1016/S0378-1119(97)00519-2
WANG LEI, WANG JUNQING, XUE LE, LI PIWU, WANG TENGFEI, SU JING, WANG RUIMING: "Construction and Application of a Vector for Marker-free Targeted Genetic Knockout in Corynebacterium Glutamicum", CHINA BREWING, ZHONGGUO NIANGZAO BIANJIBU, CN, vol. 37, no. 2, 1 January 2018 (2018-01-01), CN, pages 121 - 126, XP055815544, ISSN: 0254-5071, DOI: 10.11882/j.issn.0254-5071.2018.02.025
YU YING, XU MEI-XUE, LIU JIN-LEI, FAN RONG, FENG HUI-YONG, LI TIAN-MING: "Metabolic Engineering for Modifying Corynebacterium glutamicum to Produce More Pyruvate", BIOTECHNOLOGY BULLETIN, vol. 32, no. 8, 1 January 2016 (2016-01-01), pages 226 - 232, XP055815546, DOI: 10.13560/j.cnki.biotech.bull.1985.2016.08.033
LI TIEMIN, HU YONG-FEI, FENG QIAN-NI, LI YU, DONG ZHAN-SHUANG, LIU ZHONG-SHUN, LUO EN-JIE: "Functional Activity Analysis of CglI Gene in E.coli Strain", WEISHENGWUXUE ZAZHI - ACTA MICROBIOLOGICA SINICA, LIAONING SHENG WEISHENGWU YANJIUSUO, CN, vol. 26, no. 2, 1 March 2006 (2006-03-01), CN, pages 41 - 44, XP055815549, ISSN: 1005-7021
Attorney, Agent or Firm:
GUANGZHOU KEFENG INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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