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Title:
CORYNEBACTETIUM CONSTITUTIVE EXPRESSION VECTOR PROMOTER SCREENED ON THE BASIS OF TRANSCRIPTOME SEQUENCING, SCREENING METHOD THEREFOR, AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/171488
Kind Code:
A1
Abstract:
Provided is a method for screening a corynebacterium constitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are the corynebacterium constitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cell corynebacterium glutamicum using the expression vector, and applications thereof.

Inventors:
XING PANPAN (CN)
SU HAIXIA (CN)
WANG JIONG (CN)
MEI XUECHEN (CN)
WAN KUN (CN)
SONG MENGJUN (CN)
LI JING (CN)
LIU AIFU (CN)
Application Number:
PCT/CN2018/079034
Publication Date:
September 27, 2018
Filing Date:
March 14, 2018
Export Citation:
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Assignee:
WUHAN GRAND HOYO CO LTD (CN)
International Classes:
C12N15/113; C12N1/21; C12N15/77; C12P13/06; C12R1/15
Foreign References:
CN107164369A2017-09-15
CN107164370A2017-09-15
CN107164371A2017-09-15
CN101985631A2011-03-16
CN201710169454A2017-03-21
CN201710169685A2017-03-21
CN201710169693A2017-03-21
CN200910210962A2009-11-12
Other References:
YU, XIAOXIA: "Research Progress of Bacillus Subtilis Expression System and its Promoter Regulatory Elements", BIOTECHNOLOGY BULLETIN, vol. 31, no. 2, 1 February 2015 (2015-02-01), pages 35 - 44, XP055638209, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2015.02.005
CHENG, XIAOXUE: "Analysis the Intensity Regulated by Inducible Promoters in Corynebacterium Glutamicum ATCC13032", JOURNAL OF SHENYANG PHARMACEUTICAL UNIVERSITY, vol. 34, no. 2, 28 February 2017 (2017-02-28), pages 169 - 175, XP055638213, ISSN: 1006-2858, DOI: 10.14066/j.cnki.cn21-4349/r.2007.02.013
DATABASE Nucleotide [O] 5 January 2017 (2017-01-05), "Corynebacterium glutamicum strain XV chromosome, complete genome", XP055540837, Database accession no. CP018175
JENSEN PR ET AL., APPL ENVIRON MICROBIOL, vol. 64, no. 1, 1998, pages 82 - 87
YANG LIU ET AL., PROGRESS IN NEW ENERGY, vol. 5, no. 2, 2014, pages 353 - 357
See also references of EP 3604530A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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