Title:
CORYNEFORM BACTERIUM TRANSFORMANT AND METHOD FOR PRODUCING PHENOL USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/067174
Kind Code:
A1
Abstract:
Provided is a transformant having phenol-producing capability, wherein the gene that encodes an enzyme having tyrosine phenol-lyase activity has been introduced to a host Coryneform bacterium. Also provided is a method for producing phenol comprising: a step for reacting this transformant in a reaction solution containing tyrosine, a salt thereof, or an ester thereof under reduction conditions; and a step for recovering the phenol in a reaction culture medium.
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Inventors:
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
INUI MASAYUKI (JP)
Application Number:
PCT/JP2011/076484
Publication Date:
May 24, 2012
Filing Date:
November 17, 2011
Export Citation:
Assignee:
GREEN PHENOL TECHNOLOGY RES ASS (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
International Classes:
C12N1/21; C07C37/00; C07C39/04; C12N15/09; C12P7/22
Domestic Patent References:
| WO2009154122A1 | 2009-12-23 | |||
| WO1990004031A1 | 1990-04-19 | |||
| WO2005010182A1 | 2005-02-03 |
Foreign References:
| JPH03240492A | 1991-10-25 | |||
| JP2005333885A | 2005-12-08 | |||
| JP2007514430A | 2007-06-07 | |||
| JP2007117078A | 2007-05-17 | |||
| JP2006320238A | 2006-11-30 | |||
| JPH08154675A | 1996-06-18 | |||
| JP2005278453A | 2005-10-13 | |||
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| US6303383B1 | 2001-10-16 | |||
| JPH057491A | 1993-01-19 | |||
| JP2006124440A | 2006-05-18 |
Other References:
WIERCKX N J P ET AL.: "Engineering of Solvent- Tolerant Pseudomonas putida S12 for Bioproduction of Phenol from Glucose", APPL ENVIRON MICROBIOL., vol. 71, 2005, pages 8221 - 8227, XP055082366
ANTSON A A ET AL.: "Three-Dimensional Structure of Tyrosine Phenol-lyase", BIOCHEMISTRY, vol. 32, 1993, pages 4195 - 4206, XP055108184
DATABASE GENBANK [online] 14 April 2010 (2010-04-14), LUCAS S ET AL.: "Desulfitobacterium hafniense DCB-2 chromosome, complete genome", retrieved from http://www.ncbi.nlm. nih.gov/nuccore/219666071?sat=14&satkey= 5584935 Database accession no. NC_011830
DATABASE GENBANK [online] 20 December 2007 (2007-12-20), COPELAND A ET AL.: "Chloroflexus aurantiacus J-10-fl, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/163666892?sat=14&satkey=4111951 Database accession no. CP000909
DATABASE GENBANK [online] 24 April 2008 (2008-04-24), COPELAND A ET AL.: "Nostoc punctiforme PCC 73102, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/186463002?sat=14&satkey=4112621 Database accession no. CP001037
DATABASE GENBANK [online] 5 March 2010 (2010-03-05), SESHADRI R ET AL.: "Treponema denticola ATCC 35405, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/ae017226 Database accession no. AE017226
TOVER A ET AL.: "Growth medium composition- determined regulatory mechanisms are superimposed on CatR-mediated transcription from the pheBA and catBCA promoters in Pseudomonas putida", MICROBIOLOGY, vol. 147, 2001, pages 2149 - 2156, XP055108188
KIRCHNER U ET AL.: "Phenol Hydroxylase from Bacillus thermoglucosidasius A7, a Two-protein Component Monooxygenase with a Dual Role for FAD", J BIOL CHEM., vol. 278, 2003, pages 47545 - 47553, XP055108191
BUNJI MARUO ET AL.: "Koso Handbook", 10 October 1995, pages: 180, XP008169709
SMITH HQ ET AL.: "The tpl Promoter of Citrobacter freundii Is Activated by the TyrR Protein", J BACTERIOL., vol. 179, 1997, pages 5914 - 5921, XP055108193
See also references of EP 2641964A4
"Bergey's Manual of Determinative Bacteriology", vol. 8, 1974, pages: 599
LIEBL, W. ET AL.: "Transfer of Brevibacterium divaricatum DSM 20297T, ''Brevibacterium flavum'' DSM 20411, ''Brevibacterium lactofermentum'' DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns", INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255 - 260
KAZUO KOMAGATA ET AL.: "Classification of the coryneform group of bacteria", FERMENTATION AND INDUSTRY, vol. 45, 1987, pages 944 - 963
"Molecular Cloning, A Laboratory Manual", vol. 2, 1989, pages: 11
"Materials and Methods", J. BIOL. CHEM., vol. 245, 1970, pages 1767 - 1772
MIWA, K. ET AL.: "Cryptic plasmids in glutamic acid-producing bacteria", AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903
YAMAGUCHI, R. ET AL.: "Determination of the complete nucleotide sequence of the Brevibacterium lactofermentum plasmid pAM330 and the analysis of its genetic information", NUCLEIC ACIDS SYMP. SER., vol. 16, 1985, pages 265 - 267
KURUSU, Y. ET AL.: "Identification of plasmid partition function in coryneform bacteria", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 759 - 764
KATSUMATA, R. ET AL.: "Protoplast transformation of glutamate-producing bacteria with plasmid DNA", J. BACTERIOL., vol. 159, 1984, pages 306 - 311
EIKMANNS, B. J. ET AL.: "A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing", GENE, vol. 102, 1991, pages 93 - 98
KURUSU, Y. ET AL.: "Electroporation-transformation system for Coryneform bacteria by auxotrophic complementation", AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
VERTES A.A. ET AL.: "Presence of mrr- and mcr-like restriction systems in Coryneform bacteria", RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
INUI, M. ET AL.: "Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions", J. MOL. MICROBIOL. BIOTECHNOL., vol. 7, 2004, pages 182 - 196
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
PFENNIG, N. ET AL.: "The Prokaryotes, A Handbook on Habitats, Isolation and Identification of Bacteria", 1981, SPRINGER VERLAG, article "The dissimilatory sulfate-reducing bacteria", pages: 926 - 940
"Nogeikagaku Jikkensho", vol. 3, 1990, SANGYO TOSHO
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
ANTSON A A ET AL.: "Three-Dimensional Structure of Tyrosine Phenol-lyase", BIOCHEMISTRY, vol. 32, 1993, pages 4195 - 4206, XP055108184
DATABASE GENBANK [online] 14 April 2010 (2010-04-14), LUCAS S ET AL.: "Desulfitobacterium hafniense DCB-2 chromosome, complete genome", retrieved from http://www.ncbi.nlm. nih.gov/nuccore/219666071?sat=14&satkey= 5584935 Database accession no. NC_011830
DATABASE GENBANK [online] 20 December 2007 (2007-12-20), COPELAND A ET AL.: "Chloroflexus aurantiacus J-10-fl, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/163666892?sat=14&satkey=4111951 Database accession no. CP000909
DATABASE GENBANK [online] 24 April 2008 (2008-04-24), COPELAND A ET AL.: "Nostoc punctiforme PCC 73102, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/186463002?sat=14&satkey=4112621 Database accession no. CP001037
DATABASE GENBANK [online] 5 March 2010 (2010-03-05), SESHADRI R ET AL.: "Treponema denticola ATCC 35405, complete genome", retrieved from http://www.ncbi.nlm.nih. gov/nuccore/ae017226 Database accession no. AE017226
TOVER A ET AL.: "Growth medium composition- determined regulatory mechanisms are superimposed on CatR-mediated transcription from the pheBA and catBCA promoters in Pseudomonas putida", MICROBIOLOGY, vol. 147, 2001, pages 2149 - 2156, XP055108188
KIRCHNER U ET AL.: "Phenol Hydroxylase from Bacillus thermoglucosidasius A7, a Two-protein Component Monooxygenase with a Dual Role for FAD", J BIOL CHEM., vol. 278, 2003, pages 47545 - 47553, XP055108191
BUNJI MARUO ET AL.: "Koso Handbook", 10 October 1995, pages: 180, XP008169709
SMITH HQ ET AL.: "The tpl Promoter of Citrobacter freundii Is Activated by the TyrR Protein", J BACTERIOL., vol. 179, 1997, pages 5914 - 5921, XP055108193
See also references of EP 2641964A4
"Bergey's Manual of Determinative Bacteriology", vol. 8, 1974, pages: 599
LIEBL, W. ET AL.: "Transfer of Brevibacterium divaricatum DSM 20297T, ''Brevibacterium flavum'' DSM 20411, ''Brevibacterium lactofermentum'' DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns", INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255 - 260
KAZUO KOMAGATA ET AL.: "Classification of the coryneform group of bacteria", FERMENTATION AND INDUSTRY, vol. 45, 1987, pages 944 - 963
"Molecular Cloning, A Laboratory Manual", vol. 2, 1989, pages: 11
"Materials and Methods", J. BIOL. CHEM., vol. 245, 1970, pages 1767 - 1772
MIWA, K. ET AL.: "Cryptic plasmids in glutamic acid-producing bacteria", AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903
YAMAGUCHI, R. ET AL.: "Determination of the complete nucleotide sequence of the Brevibacterium lactofermentum plasmid pAM330 and the analysis of its genetic information", NUCLEIC ACIDS SYMP. SER., vol. 16, 1985, pages 265 - 267
KURUSU, Y. ET AL.: "Identification of plasmid partition function in coryneform bacteria", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 759 - 764
KATSUMATA, R. ET AL.: "Protoplast transformation of glutamate-producing bacteria with plasmid DNA", J. BACTERIOL., vol. 159, 1984, pages 306 - 311
EIKMANNS, B. J. ET AL.: "A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing", GENE, vol. 102, 1991, pages 93 - 98
KURUSU, Y. ET AL.: "Electroporation-transformation system for Coryneform bacteria by auxotrophic complementation", AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
VERTES A.A. ET AL.: "Presence of mrr- and mcr-like restriction systems in Coryneform bacteria", RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
INUI, M. ET AL.: "Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions", J. MOL. MICROBIOL. BIOTECHNOL., vol. 7, 2004, pages 182 - 196
OMUMASABA, C. A. ET AL.: "Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation", J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 91 - 103
PFENNIG, N. ET AL.: "The Prokaryotes, A Handbook on Habitats, Isolation and Identification of Bacteria", 1981, SPRINGER VERLAG, article "The dissimilatory sulfate-reducing bacteria", pages: 926 - 940
"Nogeikagaku Jikkensho", vol. 3, 1990, SANGYO TOSHO
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159
J. MOL. MICROBIOL. BIOTECHNOL., vol. 8, 2004, pages 243 - 254
AGRIC. BIOL. CHEM., vol. 54, 1990, pages 443 - 447
RES. MICROBIOL., vol. 144, 1993, pages 181 - 185
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
Tatsu Iwatani (JP)
Tatsu Iwatani (JP)
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