[DESCRIPTION]

[Invention Title]

A COSMETIC COMPOSITION COMPRISING TISSUE CULTURED PANAX GINSENG C.

A. MEYER ADVENTITIOUS ROOT ITSELF AND A PREPARING METHOD THEREOF

[Technical Field]

The present invention relates to a cosmetic composition comprising

tissue-cultured adventitious roots itself of a ginseng, and to a preparing

method thereof. This application claims priority from Korean Patent

Application No. 10-2007-0005237 filed on January 17, 2007 in the Korean

Intellectual Property Office, the disclosure of which is incorporated

herein by reference in its entirety.

[Background Art]

Generally, functional cosmetics comprising a plant extract is

prepared through multiple steps such as seeding, culturing, harvesting,

washing, drying, dipping, aging, and filtering for a cosmetic composition.

Therefore, there is a drawback that the process for preparing the cosmetics

requires long time and high cost.

It is possible that a large amount of ingredients having a high

purity can be obtained in short time in the case of an extraction or

supercritical extraction method using a solvent . However, the ingredients

extracted by the extraction method have problems in their stability such

that they can be precipitated due to light, air, temperature or the like,

or their colors can be changed with the passage of time. Accordingly, upon

adding the ingredients to a cosmetic composition, there is caused a problem

that they may affect the properties of the cosmetic composition.

Since the extracts are typically obtained through dipping or aging

for a short time without aging for a sufficient period, they have a problem

that a sufficient effect of extraction of effective ingredients is hardly

expected. Further, since the effective ingredients cannot be extracted

in a large amount without further isolation and/or purification step,

cosmetics comprising a high concentration of the effective ingredients

is hardly prepared with the extract itself.

A consumer to purchase functional cosmetics cannot recognize

visually the presence and contents of an extract containing the effective

ingredients, and cannot but purchase and use depending on only labeled

ingredients on the package of a cosmetic.

In order to improve the above-described problems, a preparing

method for cosmetics comprising a plant itself, not a plant extract, was

proposed. However, the method has a drawback that cost is required for

sterilizing the plant and a product having uniform quality cannot be

achieved due to its different size, harvesting places and periods of the

plant added to composition.

A cosmetic composition comprising an undifferentiated callus using

a recent tissue culture technique was disclosed in Korean Patent No.637342.

A callus means a cell mass of which function is not determined. Accordingly,

a callus has been known to have different types and contents of effective

ingredients from those of the differentiated plants.

A korean wild ginseng ("Sansam"), which is an example of a plant

containing a large amount of a functional ingredient, belongs to Panax

ginsengC. A. Meyer (hereinafter "ginseng") of the Araliacea family, and

grows natural Iy in a mountain. A korean wi Id ginseng belongs to a flowering

plant and angiosperms, in which a peduncle grows with leafs and a stalk

upon budding, and to a semi-shade plant.

A korean wild ginseng has been recorded as a "mysterious herb" in

Dongeui Bogam (the integration of traditional Korean medicine).

Traditionally, it has been thought to be a celebrated medicinal herb as

a rare herb, and as a result, it has been used as a therapeutic agent for

various diseases and a nutrient supplement.

The main component of a ginseng is a glycoside. The mixture of

glycosides is hydrolyzed with an acid to give a free sugar such as glucose,

rhamnose, sucrose and fructose, and aglycone, and to give panaxadiol and

panaxatriol, β-sistosterol and oleanolic acid as a saponin glycoside.

Panacene, which is a sesquiterpenoid compound, is an aromatic component.

Further, it has been known that other components, such as β-sistosterol,

stigumasterol ; alkaloid; flavonoids such as kaempherol, trifolime, and

panasenoid; vitamins such as B complex, nicotinic acid, pantothenic acid,

folic acid and biotin; trace elements such as manganese, copper, vanadium,

cobalt, arsenic, germanium, phosphorus, aluminum and nickel ; germanium

cell enzyme; and enzymes such as amylase, and phenolase, are contained.

According to the results from clinical research, it has been found

that a ginseng has an efficacy such as antidiabetic effect, anticancer

effect, cardio-stimulation, regulation of blood pressure, stimulation of

liver function, stimulation of stomach, stress relief and restorative

activity, stamina enhancement , stimulation of brain function, anti-aging

activity, radiation protection, effect on anemia and hematopoiesis,

immunity enhancement, ant i-inflammatory effect and asthenia improving

effect. The efficacy of the korean wild ginseng is similar to that of a

cultivated ginseng, but the korean wild ginseng is more excellent with

respect to the pharmacological effect.

Generally, a korean wild ginseng is divided into four categories

in Korea, i.e. ("Cheonjong, Jijong, Injong, Jangnoi"). Specifically,

Cheonjong (natural wildginseng) naturally occurs from wild seeds; Jijong

is propagated, for which animals or birds eat and excrete their seeds;

Injong is cultured by human! and Jangnoi (wild simulated ginseng) is

obtained by collecting the seed, seeding in the mountain, and artificially

growing it in a wild state. A seed of the wild ginseng does not easily

germinate, and even if the seed sprouts or germinates, it can survive just

few years . Therefore, the wi Id ginseng is a rare plant which it is difficult

to culture.

Recently, to solve the drawbacks such as a rareness of the wild

ginseng and high value-added of the products, a method for

mass-propagating the adventitious roots using the root tissue of a wild

ginseng and the technique of the plant tissue culture had been known.

Accordingly, the adventitious roots of a wild ginseng having uniform and

excellent quality can be cultured in a large amount and a short period

comparatively, which will be used for various foods or as a raw material.

For example, in the case of preparing an alcoholic beverage, a cultivated

ginseng, a Japanese apricot , gold was added and aged to improve its flavor,

and a cultivated ginseng itself was contained in a beverage to enhance

trust for the product with visual effect. However, a cosmetic composition

comprising adventitious roots itself of a wild ginseng has not been

published or disclosed yet.

Therefore, the inventors of the present invention have made

extensive studies on preparation of a cosmetic composition, which stably

comprises effective ingredients of a ginseng. They have found that the

ingredient dip-extracted from the adventitious roots of a ginseng was

uniformly maintained by adding a tissue-cultured adventitious roots

itself of a ginseng to a cosmetic composition, and that user satisfaction

was high in a sensory test, thereby completing the present invention.

[Disclosure]

[Technical Problem]

Accordingly, it is an object of the present invention to provide

a cosmetic composition comprising tissue-cultured adventitious roots

itself of a ginseng, and a preparing method thereof , in which the effective

ingredients are dip-extracted from the tissue-cultured adventitious roots

itself of a ginseng and the content thereof were continuously maintained,

and also a user recognizes visually the presence of the adventitious roots

of a ginseng, thereby giving a user trust of the presence of the effective

ingredient.

[Technical Solution]

In order to achieve the object, the present invention provides a

cosmetic composition comprising tissue-cultured adventitious roots

itself of a ginseng and cosmetic ingredients.

The cosmetic composition of the invention comprises:

0.1 to 5 percent by weight of the tissue-cultured

adventitious roots itself of the ginseng, based on the total weight of

the cosmetic composition; and

95 to 99.9 percent by weight of the cosmetic ingredients,

based on the total weight of the cosmetic composition.

The ginseng of the invention can be selected from the group

consisting of a wild ginseng ("Sansam"), a cultivated ginseng ("Insam"),

and a wild simulated ginseng ("Jangnoi").

The cosmetic ingredients of the invention is selected from the group

consisting of a solvent, an extender, an inorganic pigment, an organic

pigment, an inorganic powder, an emulsion, a surfactant compatible a

cosmetic composition, a silicone-contained surfactant, a thickener, a

stabilizer, a preservative, an antioxidant, a flavoring agent, other

cosmetic ingredients conventionally known in the art, and combinations

thereof.

The composition is characterized in that, in the case of the

composition comprises, as a solvent, 1 to 5 percent by weight of polyol

based on the total weight of the composition, the ingredients that are

effective for moisturization are dip-extracted from the blended

tissue-cultured adventitious roots of a ginseng.

The composition is characterized in that, in the case of the

composition comprises, as a solvent, 5 to 30 percent by weight of polyol

based on the total weight of the composition, the ingredients that are

effective for anti-wrinkles are dip-extracted from the blended

tissue-cultured adventitious roots of a ginseng.

The composition is characterized in that, in the case of the

composition comprises, as a solvent: 5 to 30 percent by weight of polyol

based on the total weight of the composition; and 1 to 50 percent by weight

of alcohol having 1 to 4 carbon atom based on the total weight of the

composition, the ingredients that are effective for whitening are

dip-extracted from the blended tissue-cultured adventitious roots of a

ginseng.

The present invention further provides a preparing method for a

cosmetic composition comprising tissue-cultured adventitious roots

itself of a ginseng, comprising:

mixing conventional cosmetic ingredients; and

adding tissue-cultured adventitious roots itself of a

ginseng to the cosmetic ingredient mixture.

A cosmetic composition of the present invention comprises

ingredients that are effective for moisturization, anti-wrinkles, and

whitening.

[Advantageous Effects]

The cosmetic composition of the present invention has advantages

that the effective ingredients dip-extracted from tissue-cultured

adventitious roots of a ginseng and the content thereof were continuously

maintained, and also a user recognizes visually the presence of the

adventitious roots itself of a ginseng, thereby improving a user's trust

and satisfaction. Further, the preparing method for the cosmetic

composition is a simple and inexpensive process without any additional

step, such as extraction, isolation, and purification, and further has

an advantage that the effective ingredients are dip-extracted from the

adventitious roots itself of ginseng while not losing the effective

ingredients.

[Description of Drawings]

Fig. 1 is a photograph of the tissue-cultured adventitious roots

of a korean wild ginseng.

Fig. 2 is a graph describing each preference for a cosmetics

comprising tissue-cultured adventitious roots itself of a korean wild

ginseng, and a cosmetics comprising the extract of a tissue-cultured

adventitious roots of a wild ginseng.

Fig. 3 is a photograph of a cream and a serum, each comprising

tissue-cultured adventitious roots itself of a korean wild ginseng, which

correspond to Examples according to the present invention.

[Mode for invention]

Hereinbelow, the present invention will be described in detail.

In one embodiment of the invention, a cosmetic composition may be

prepared by a method comprising:

mixing conventional cosmetic ingredients; and

adding tissue-cultured adventitious roots itself of a

ginseng dried with hot air at 50 to 60 ^° C to the cosmetic ingredient mixture.

In another embodiment of the invention, the cosmetic ingredients

may be mixed by a method conventionally known by a skilled person in the

art.

In another embodiment of the invention, the tissue-cultured

adventitious roots of a ginseng is characterized in that it is obtained

by the tissue culture method disclosed in Korean patent No. 0353636.

Preferably, the cosmetic composition of the invention comprises:

0.1 to 5 percent by weight of the tissue-cultured adventitious roots of

a ginseng, based on total weight of the cosmetic composition. If the

content of the adventitious roots of a ginseng is less than 0.1 percent

by weight, the content of effective ingredients dip-extracted becomes

insufficient. If the content of the adventitious roots of a ginseng is

more than 5 percent by weight, the states and properties of the product

such as viscosity can be deteriorated, and stimulation on a user can be

caused.

The cosmetic composition of the invention is characterized in that ,

in the case of the composition comprises polyol, as a solvent, preferably

1 to 5 percent by weight of 1,3-butyleneglycol based on total weight of

the composition, ingredients that are effective for moisturization,

preferably carbohydrates, are dip-extracted from the blended adventitious

roots of a ginseng.

The cosmetic composition of the invention is characterized in that ,

in the case of the composition comprises polyol, as a solvent, preferably

5 to 30 percent by weight of 1,3-butyleneglycol based on total weight of

the composition, ingredients that are effective for ant i-wrinkles,

preferably vitamins or saponins, are dip-extracted from the blended

adventitious roots of a ginseng.

The cosmetic composition of the invention is characterized in that ,

in the case of the composition comprises polyol and alcohol having 1 to

4 carbon atom, as a solvent, preferably 5 to 30 percent by weight of

1,3-butyleneglycol and 1 to 50 percent by weight of ethanol based on total

weight of the composition, ingredients that are effective for whitening,

preferably flavonoids, are dip-extracted from the blended adventitious

roots of a ginseng.

The cosmetic composition of the invention is found to contain 70%

or more of the effective ingredients dip-extracted from the adventitious

roots of a ginseng from 30 days after preparation, and reduction in the

content of the effective ingredients is not observed until 180 days after

aging. Thus, it can be used for a functional cosmetic composition.

If the tissue-cultured adventitious roots itself of a ginseng

contained in the cosmetic composition of the invention is contaminated

by various microorganisms, its color and phase may be changed. Accordingly,

it can be an index that indicates contamination, and the cosmetic

composition of the invention can be used with safety.

In the cosmetic composition of the present invention, the effective

ingredients are naturally and continuously dip-extracted from the

tissue-cultured adventitious roots of a ginseng, and maintained, and also

allow a user to recognize visually the presence of the adventitious roots

itself of a ginseng, thereby giving trust of the presence of the effective

ingredient to the user, and improving user' s satisfaction through its

natural flavor.

The cosmetic composition of the invention can be used for various

products, such as cosmetics, a facial cleanser, and a shampoo, for

moisturizing, anti-wrinkle or whitening. The cosmetic composition can be

added to various products, including a skin lotion, a skin softener, a

skin toner, an astringent toner, a lotion, amilk lotion, anutrient lotion,

a massage cream, a nutrient cream, a hand cream, a foundation, a serum,

a nutrient serum, a pack, a soap, a cleansing foam, a cleansing lotion,

a cleansing cream, a body lotion or a body cleanser, but not limited

thereto.

Hereinbelow, the present invent ion wi 11 be described in detail with

reference to Examples and Experimental Examples.

Examples and Experimental Examples are provided only for the

purpose of illustrating the present invention, and accordingly, it is not

intended that the present invention is limited thereto.

Reference Example. Preparation of the tissue-cultured adventitious roots

of a korean wild ginseng

The tissue-cultured adventitious roots of a korean wild ginseng

used in the present invention was purchased from CBN biotech (Korea). The

adventitious roots of a korean wild ginseng were mass-propagated through

tissue culture according to the method disclosed in Korean Patent No.

353636, and was dried with hot air at 50 to 60 ^° C before using.

Example 1. Preparation of moisturizing serum comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

Amoisturizing serum comprising tissue-cultured adventitious roots

itself of a korean wild ginseng was prepared according to the composition

as shown in the following Table 1.

[Table 1]

A was sufficiently dispersed and moisturized to be in a uniform gel

state, and B was added thereto for neutralization. After C was added to

(A+B) to solubilize with uniform stirring, D was added thereto at room

temperature. The mixture was stirred in order to disperse uniformly, and

then was put into a container for commercialization. When the serum

prepared by the above method was applied to skin, its moisturizing effect

was continuously maintained.

Example 2. Preparation of moisturizing cream comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

Amoisturizing cream comprising tissue-cultured adventitious roots

itself of a korean wild ginseng was prepared according to the composition

as shown in the following Table 2.

[Table 2]

Ingredients

1,3-Butyleneglycol

Glycerine

!arbomer glyceryl methacrylate

Paraben

The cream in Example 2 was prepared in the same manner as in Example

1. When the cream prepared by the above method was applied to skin, its

moisturizing effect was continuously maintained.

Example 3. Preparation of anti-wrinkle serum comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

An anti-wrinkle serum comprising tissue-cultured adventitious

roots itself of a korean wild ginseng was prepared according to the

composition as shown in the following Table 3.

[Table 3]

The serum in Example 3 was prepared in the same manner as in Example

1. When the serum prepared by the above method was applied to skin, its

anti-wrinkle effect was continuously maintained.

Example 4. Preparation of anti-wrinkle cream comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

An anti-wrinkle cream comprising tissue-cultured adventitious

roots itself of a korean wild ginseng was prepared according to the

composition as shown in the following Table 4.

[Table 4]

The cream in Example 4 was prepared in the same manner as in Example

1. When the cream prepared by the above method was applied to skin, its

anti-wrinkle effect was continuously maintained.

Example 5. Preparation of whitening serum comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

A whitening serum comprising tissue-cultured adventitious roots

itself of a korean wild ginseng was prepared according to the composition

as shown in the following Table 5.

[Table 5]

The serum in Example 5 was prepared in the same manner as in Example

1. When the serum prepared by the above method was applied to skin, its

whitening effect was continuously maintained.

Example 6. Preparation of whitening cream comprising tissue-cultured

adventitious roots itself of a korean wild ginseng

A whitening cream comprising the tissue-cultured adventitious

roots itself of a korean wild ginseng was prepared according to the

composition as shown in the following Table 6.

[Table 6]

The cream in Example 6 was prepared in the same manner as in Example

1. When the cream prepared by the above method was applied to skin, its

whitening effect was continuously maintained.

Comparative Example 1 to 6. Preparation of cosmetic composition not

comprising tissue-cultured adventitious roots itself of a korean wild

ginseng

A cosmetic composition not comprising tissue-cultured adventitious

roots itself of a korean wild ginseng was prepared with the same

ingredients as in Example 1 to 6, except an tissue-cultured adventitious

roots of a korean wild ginseng.

Experimental Example 1. Efficacy test of cosmetic composition according to

present invention

1-1. Test for moisturizing effect

To confirm the moisturizing effect of the cosmetic composition

according to the present invention, the hydration level in skin was

measured. Each cosmetic compositions prepared according to the

formulations in Examples 1 and 2, and Comparative Examples 1 and 2 was

applied to twenty female subjects, who were 20 to 50 years old. The

hydration level in skin was measured before and after applying each

cosmetic composition as following.

The hydration level in skin was measured by means of a corneometer

(CM 825, Courage & Khazaka, Germany), which is a conventional skin

hydration measurement device using capacitance measurement of a

dielectric medium. Each sample of 2.0 nig/cnf was uniformly applied to one

upper arm of subjects, who were waiting for 1 hour before test. Each sample

was not applied to the other upper arm of subjects, as a control group.

The hydration level in skin was measured immediately before and after

applying each sample. Further, the moisture content in skin was measured

in 1 hour, 3 hours, and 5 hours, after applying each sample, respectively.

[Table 7]

[Table 8]

As shown in Tables 7 and 8, there is a significant difference in

the hydration level between the portions which were applied with the

cosmetic compositions of Example 1 or 2 and those which were not applied.

The hydration level in the portions which were applied with the

cosmetic compositions of Example 1 or 2 in more than 30 days after

preparation was excellent, compared with those in the portions which were

applied with the cosmetic compositions of Comparative Example 1 or 2. It

was also found that the hydration level in the portions which were applied

with the cosmetic compositions of Example 1 or 2 in 180 days after

preparation were excellently maintained.

1-2. Test for effect of promoting collagen biosynthesis

To confirm the anti-wrinkle effect of the cosmetic composition

according to the present invention, the effect of promoting collagen

biosynthesis was measured using fibroblasts. Mouse fibroblast (3T3-L1,

KCLB 10092.1, Korean Cell Line Bank) were cultured using DMEM medium

(Dulbecco's Modified Eagle's Medium, Invitrogen, USA) containing 10 %

(v/v) FBS (fetal bovine serum, BioWhittaker, MD, USA) and 1% (v/v)

penicillin-streptomycin (BioWhittaker, MD, USA) under the condition of

5% CO2 and 37°C . After eel 1 culture, the eel Is were col lected with treat ing

trypsin. The collected cells were seeded in a 24-well plate at a

concentration of IxIO ⁵ cells/in-C/well , followed by culturing for 24 hours.

After cell culture, each cosmetic compositions prepared according to the

formulations in Example 3 and 4, and Comparative Examples 3 and 4 were

diluted to 0.01%. The cells were treated with the diluted cosmetic

compositions and cultured for 24 hours, followed by using for the following

experiment .

In order to confirm whether collagen biosynthesis was enhanced in

the fibroblast or not, the supernatant of the cultured cells was collected

to determine the amount of collagen.

Specifically, 1 mi of a dye reagent (prepared by dissolving 0.1 g

of a sirius red reagent (BDH, UK) in 100 ml of saturated picric acid) was

added to 100 μi of the supernatant. The mixture was subjected to reaction

under slow stirring at room temperature for 30 minutes. Sirius red is an

anionic pigment, which specifically binds to collagen. After 30 minutes

reaction, a collagen-pigment complex was precipitated by a centrifuge at

over 5000Xg for 20 minutes.0.5 M of NaOH was added to the precipitate

to dissolve at room temperature for 5 minutes. Absorbance of the solution

was measured using a micro well plate reader at 540 nm. The result was

shown in Tables 9 and 10. The results were compared with the absorbance

values of the non-test group to evaluate the effect of promoting collagen

biosynthesis.

[Table 9]

føtio of collagen

Sample biosynthesis

Comparative Example 3 101

Example 3 (in 30 days after preparation) 108 %

Example 3 (in 60 days after preparation) 110 %

Ixample 3 (in 90 days after preparation) 118 %

Example 3 (in 120 days after preparation) 121

Ixample 3 (in 150 days after preparation) 118

[Example 3 ( in 180 days after preparat ion) 118 [Table 10]

in

a ratio of collagen biosynthesis between the fibroblast treated with the

cosmetic compositions of Example 3 or 4 and the fibroblast treated with

the cosmetic compositions of Comparative Example 3 or 4.

A ratio of collagen biosynthesis increased in, from the group which

was treated with a cosmetic composition in 30 days after preparation, to

the group which was treated with a cosmetic composition in 180 days after

preparation. It was found that the ratio of collagen biosynthesis of the

groups treated with the cosmetic compositions maintained 1.2 times than

that of a control group.

1-3. Test for effect of inhibiting tyrosinase activity

To confirm the whitening effect of the cosmetic composition

according to the present invention, the effect of inhibiting tyrosinase

activity was measured. A mushroom tyrosinase (T-3824, 1530U/ing, Sigma)

was dissolved in a phosphate buffer (pH 6.5) to the concentration of 1000

U/in-d to prepare a tyrosinase enzyme solution. L-tyrosine (45160-0410,

Junsei chemical co. Ltd) was dissolved in a phosphate buffer (pH 6.5) to

the concentration of 1.5 mM to prepare a substrate solution.

Each cosmetic compositions prepared according to the formulations

in Example 5 and 6, and Comparative Example 5 and 6 was added to 20 g of

purified water and stirred to be a total volume of 500 ml. 1 N of

hydrochloric acid was added thereto to adjust the pH to 2.0. The solution

was placed in a separatory funnel . The equal amount of chloroform was added

thereto. The solution was vigorously shaken and left to separate an aqueous

layer. After the operation was repeated twice times, the obtained aqueous

layer was concentrated under reduced pressure at 60 ^° C . 1 N of sodium

hydroxide was added thereto to adjust the pH to 6.5. Further, purified

water was added thereto to be a volume of 20 ml,. The solution was filtered

and a phosphate buffer (pH 6.5) was added to 5 ml of filtrate to be a volume

of 10 ml as a test solution.10 μl of the tyrosinase enzyme solution was

added to 170 μl of the test solution, and left at 37°C for 10 minutes.

20 μl of the substrate solution was added thereto. The reaction was carried

out at 37°C for 10 minutes, and immediately left in ice for 5 minutes.

Absorbance was measured using an ELISA reader (UV max kinetic microplate

reader 01414, Molecular Devices, USA) at 490 nm. The absorbance measured

was substituted into Equation 1 to calculate the inhibition ratio of

tyrosinase activity. The result was shown in the following Table 11 and

12. A phosphate buffer (pH 6.5) instead of the test solution was added

to prepare a blank solution. Further, a phosphate buffer (pH 6.5) instead

of the substrate solution was added to prepare a calibration solution.

[Equation 1]

Inhibition ratio of tyrosinase activity (%) = 100-[(A-A' )/(B-B ¹ )] XlOO

A: Absorbance after reaction of test solution

B: Absorbance after reaction of blank solution

A': Calibration solution of test solution

B': Calibration solution of blank solution

[Table 11]

[Table 12]

Inhibition ratio of

Sample tyrosinase activity

Comparative Example 6 32

As shown in Tables 11 and 12, it was found that when the cosmetic

compositions in Examples 5 and 6 according to the present invention were

aged for 30 days to 180 days after preparation, effective ingredients for

whitening were dip-extracted from the adventitious roots of a korean wild

ginseng to increase the inhibition ratio of tyrosinase activity. Therefore,

it was found that the activity of the effective ingredients for whitening

was maintained.

Experimental Example 2. Preference survey for cosmetics comprising

tissue-cultured adventitious roots itself of a korean wild ginseng

A preference survey for the formulations in Example 1 and 2, and

Comparative formulations (serum and cream comprising an extract of the

adventitious roots of a wild ginseng) was performed by 100 female subjects,

who were 20 to 50 years old. The results of preference survey were shown

in Fig. 2.

As shown in Fig.2, the presence of the adventitious roots itself

of a ginseng can be visually recognized, and as a result, it can be found

that 84 females of 100 females prefer the formulation comprising the

adventitious roots itself of a wild ginseng to the formulation comprising

the extract of the adventitious roots of a wild ginseng.