Title:
COVALENT MODIFICATION OF RNA FOR IN VITRO AND IN VIVO DELIVERY
Document Type and Number:
WIPO Patent Application WO2004076630
Kind Code:
A3
Abstract:
The post-synthetic modification of RNA for the delivery of the RNA to a mammalian cell is described. The modifications enhance resistant of the RNA to nuclease digestion and delivery of the RNA to the cell whether the RNA is delivered alone or in combination with a transfection agent. Activity of the RNA in the cell is maintained.
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Inventors:
MONAHAN SEAN D
BUDKER VLADIMIR G
NADER LISA
SUBBOTIN VLADIMIR
WOLFF JON A
BUDKER VLADIMIR G
NADER LISA
SUBBOTIN VLADIMIR
WOLFF JON A
Application Number:
PCT/US2004/005096
Publication Date:
June 09, 2005
Filing Date:
February 20, 2004
Export Citation:
Assignee:
MIRUS CORP (US)
International Classes:
C12N15/11; (IPC1-7): C07H21/04; A61K48/00
Other References:
CHAMBERLIN ET AL: "Catalysis of Amide Synthesis by RNA Phosphodiester and Hydroxyl Groups", PNAS, vol. 99, 12 November 2002 (2002-11-12), pages 14688 - 14692, XP002986028
TANG X-Q. ET AL: "2'-C-Branched ribonucleosides: Synthesis of the Phosphoramidite Derivatives of 2'-C-beta-methylcytidine and Their Incorporation into Oligonucleotides", J ORG CHEM, vol. 64, 9 January 1999 (1999-01-09), pages 747 - 754, XP002156891
SINGER ET AL: "Targeted mutagenesis of DNA with Alkylating RecA assisted oligonucleotides", NUCLEIC ACIDS RESEARCH, vol. 27, 15 September 1999 (1999-09-15), pages E38, XP002986029
MASTA ET AL: "Nitrogen Mustard Inhibits Transcription and Translation in a Cell Free System", NUCLEIC ACIDS RESEARCH, vol. 23, 6 August 1995 (1995-08-06), pages 3508 - 3510, XP002986030
TANG X-Q. ET AL: "2'-C-Branched ribonucleosides: Synthesis of the Phosphoramidite Derivatives of 2'-C-beta-methylcytidine and Their Incorporation into Oligonucleotides", J ORG CHEM, vol. 64, 9 January 1999 (1999-01-09), pages 747 - 754, XP002156891
SINGER ET AL: "Targeted mutagenesis of DNA with Alkylating RecA assisted oligonucleotides", NUCLEIC ACIDS RESEARCH, vol. 27, 15 September 1999 (1999-09-15), pages E38, XP002986029
MASTA ET AL: "Nitrogen Mustard Inhibits Transcription and Translation in a Cell Free System", NUCLEIC ACIDS RESEARCH, vol. 23, 6 August 1995 (1995-08-06), pages 3508 - 3510, XP002986030
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