Title:
CRISPR-BASED COMPOSITIONS AND METHODS OF USE
Document Type and Number:
WIPO Patent Application WO/2016/100951
Kind Code:
A3
Abstract:
This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.
Inventors:
COLLINGWOOD MICHAEL ALLEN (US)
JACOBI ASHLEY MAE (US)
RETTIG GARRETT RICHARD (US)
SCHUBERT MOLLIE SUE (US)
BEHLKE MARK AARON (US)
JACOBI ASHLEY MAE (US)
RETTIG GARRETT RICHARD (US)
SCHUBERT MOLLIE SUE (US)
BEHLKE MARK AARON (US)
Application Number:
PCT/US2015/066942
Publication Date:
October 20, 2016
Filing Date:
December 18, 2015
Export Citation:
Assignee:
INTEGRATED DNA TECH INC (US)
International Classes:
C12N15/11
Domestic Patent References:
| WO2014144592A2 | 2014-09-18 | |||
| WO2014065596A1 | 2014-05-01 | |||
| WO2014124226A1 | 2014-08-14 | |||
| WO2016080097A1 | 2016-05-26 | |||
| WO2016089433A1 | 2016-06-09 |
Foreign References:
| US20140273232A1 | 2014-09-18 |
Other References:
M. JINEK ET AL: "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", SCIENCE, vol. 337, no. 6096, 17 August 2012 (2012-08-17), US, pages 816 - 821, XP055229606, ISSN: 0036-8075, DOI: 10.1126/science.1225829
REGINA CENCIC ET AL: "Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage", PLOS ONE, vol. 9, no. 10, 2 October 2014 (2014-10-02), pages e109213, XP055175713, DOI: 10.1371/journal.pone.0109213
MEGHDAD RAHDAR ET AL: "Synthetic CRISPR RNA-Cas9-guided genome editing in human cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, 16 November 2015 (2015-11-16), US, XP055264777, ISSN: 0027-8424, DOI: 10.1073/pnas.1520883112
AYAL HENDEL ET AL: "Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells", NATURE BIOTECHNOLOGY, vol. 33, no. 9, 29 June 2015 (2015-06-29), US, pages 985 - 989, XP055233915, ISSN: 1087-0156, DOI: 10.1038/nbt.3290
REGINA CENCIC ET AL: "Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage", PLOS ONE, vol. 9, no. 10, 2 October 2014 (2014-10-02), pages e109213, XP055175713, DOI: 10.1371/journal.pone.0109213
MEGHDAD RAHDAR ET AL: "Synthetic CRISPR RNA-Cas9-guided genome editing in human cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, 16 November 2015 (2015-11-16), US, XP055264777, ISSN: 0027-8424, DOI: 10.1073/pnas.1520883112
AYAL HENDEL ET AL: "Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells", NATURE BIOTECHNOLOGY, vol. 33, no. 9, 29 June 2015 (2015-06-29), US, pages 985 - 989, XP055233915, ISSN: 1087-0156, DOI: 10.1038/nbt.3290
Attorney, Agent or Firm:
CELANDER, Daniel, W. (19 North Green StreetChicago, IL, US)
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