Title:
CRISPR-CAS-BASED COMPOSITION FOR SALMONELLA DETECTION AND SALMONELLA DETECTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/163458
Kind Code:
A1
Abstract:
The present invention relates to a CRISPR-Cas-based composition for salmonella detection and a salmonella detection method using same and, more specifically, to a composition for salmonella detection comprising a primer pair capable of specifically amplifying salmonella by isothermal amplification, guide RNA, and a CRISPR-Cas protein and to a detection method using same.
Inventors:
LEE SANG YONG (KR)
PARK YOUNG MIN (KR)
KIM SANG GU (KR)
LEE SANG YOON (KR)
LEE HYO YUL (KR)
PARK YOUNG MIN (KR)
KIM SANG GU (KR)
LEE SANG YOON (KR)
LEE HYO YUL (KR)
Application Number:
PCT/KR2023/002300
Publication Date:
August 31, 2023
Filing Date:
February 16, 2023
Export Citation:
Assignee:
PULMUONE CO LTD (KR)
International Classes:
C12Q1/689; C12N9/22; C12N15/113; C12Q1/6844
Foreign References:
| CN110684823A | 2020-01-14 | |||
| KR20210143580A | 2021-11-29 | |||
| CN111621578A | 2020-09-04 |
Other References:
KIM SEUL I: "Roles of YehZ, a Putative Osmoprotectant Transporter, in Tempering Growth of Salmonella enterica serovar Typhimurium", JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, HAN'GUG MI'SAENGMUL SAENGMYEONG GONG HAGHOE,KOREAN SOCIETY FOR MICROBIOLOGY AND BIOTECHNOLOGY, KOREA, vol. 23, no. 11, 28 November 2013 (2013-11-28), Korea, pages 1560 - 1568, XP093088962, ISSN: 1017-7825, DOI: 10.4014/jmb.1308.08006
DATABASE Nucleotide 7 February 2022 (2022-02-07), ANONYMOUS : "Salmonella enterica subsp. enterica serovar Typhimurium strain FD01851333 chromosome ", XP093088966, retrieved from NCBI Database accession no. CP069164.1
ZHANG TING, LI HAI-TAO, XIA XUHAN, LIU JUN, LU YUNHAO, KHAN MOHAMMAD RIZWAN, DENG SHA, BUSQUETS ROSA, HE GUIPING, HE QIANG, ZHANG : "Direct Detection of Foodborne Pathogens via a Proximal DNA Probe-Based CRISPR-Cas12 Assay", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 43, 3 November 2021 (2021-11-03), US , pages 12828 - 12836, XP093088971, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c04663
DATABASE Nucleotide 7 February 2022 (2022-02-07), ANONYMOUS : "Salmonella enterica subsp. enterica serovar Typhimurium strain FD01851333 chromosome ", XP093088966, retrieved from NCBI Database accession no. CP069164.1
ZHANG TING, LI HAI-TAO, XIA XUHAN, LIU JUN, LU YUNHAO, KHAN MOHAMMAD RIZWAN, DENG SHA, BUSQUETS ROSA, HE GUIPING, HE QIANG, ZHANG : "Direct Detection of Foodborne Pathogens via a Proximal DNA Probe-Based CRISPR-Cas12 Assay", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 43, 3 November 2021 (2021-11-03), US , pages 12828 - 12836, XP093088971, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c04663
Attorney, Agent or Firm:
LEE, Hee Sook (KR)
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