Title:
CRISPR-CAS13 SYSTEM AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/041653
Kind Code:
A1
Abstract:
The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.
Inventors:
LIANG JUNBIN (CN)
LIANG XINGXIANG (CN)
SUN YANG (CN)
XU HUI (CN)
SI KAIWEI (CN)
LI QIUTING (CN)
PENG ZHIQIN (CN)
HUANGFU DESHENG (CN)
LIANG XINGXIANG (CN)
SUN YANG (CN)
XU HUI (CN)
SI KAIWEI (CN)
LI QIUTING (CN)
PENG ZHIQIN (CN)
HUANGFU DESHENG (CN)
Application Number:
PCT/CN2023/115093
Publication Date:
February 29, 2024
Filing Date:
August 25, 2023
Export Citation:
Assignee:
GUANGZHOU REFORGENE MEDICINE CO LTD (CN)
ZHEJIANG SYNSORBIO TECH CO LTD (CN)
ZHEJIANG SYNSORBIO TECH CO LTD (CN)
International Classes:
C12N9/22; C12N15/55
Domestic Patent References:
| WO2020160150A1 | 2020-08-06 |
Foreign References:
| US20190062724A1 | 2019-02-28 | |||
| CN113234702A | 2021-08-10 | |||
| CN113544267A | 2021-10-22 |
Other References:
DATABASE Protein 21 April 2021 (2021-04-21), ANONYMOUS : "MAG: type VI-D CRISPR-associated RNA-guided ribonuclease Cas13d [Thermoguttaceae bacterium] ", XP093142112, retrieved from NCBI Database accession no. MBR0191107.1
"Master’s Dissertations", 18 July 2020, FUJIAN NORMAL UNIVERSITY, China, article YE, YANGMIAO: "Structural Insights into CRISPR-Cas13d Effector", XP009552797, DOI: 10.27019/d.cnki.gfjsu.2020.002005
GUPTA RAHUL, GHOSH ARIJIT, CHAKRAVARTI RUDRA, SINGH RAJVEER, RAVICHANDIRAN VELAYUTHAM, SWARNAKAR SNEHASIKTA, GHOSH DIPANJAN: "Cas13d: A New Molecular Scissor for Transcriptome Engineering", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, FRONTIERS MEDIA, CH, vol. 10, 31 March 2022 (2022-03-31), CH , pages 866800, XP093142116, ISSN: 2296-634X, DOI: 10.3389/fcell.2022.866800
"Master’s Dissertations", 18 July 2020, FUJIAN NORMAL UNIVERSITY, China, article YE, YANGMIAO: "Structural Insights into CRISPR-Cas13d Effector", XP009552797, DOI: 10.27019/d.cnki.gfjsu.2020.002005
GUPTA RAHUL, GHOSH ARIJIT, CHAKRAVARTI RUDRA, SINGH RAJVEER, RAVICHANDIRAN VELAYUTHAM, SWARNAKAR SNEHASIKTA, GHOSH DIPANJAN: "Cas13d: A New Molecular Scissor for Transcriptome Engineering", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, FRONTIERS MEDIA, CH, vol. 10, 31 March 2022 (2022-03-31), CH , pages 866800, XP093142116, ISSN: 2296-634X, DOI: 10.3389/fcell.2022.866800
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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