Title:
CRY2 VARIANT HAVING INCREASED PHOTOSENSITIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/101355
Kind Code:
A1
Abstract:
Provided is a CRY2 variant in which E279 and/or E281 having a negative charge in sequence N277SEGE281 of SEQ ID NO: 1 is substituted with any one selected from the neutral amino acid group consisting of alanine (A), isoleucine (I), leucine (L), valine (V), phenylalanine (F), methionine (M) and tryptophan (W), or in which S278 or G280 of sequence N277SEGE281 of SEQ ID NO: 1 is substituted with any one selected from the bulky amino acid group consisting of tryptophan (W) and phenylalanine (F).
Inventors:
HEO WONDO (KR)
KIM SUNGSOO (KR)
LEE SANG KYU (KR)
KYUNG TAEYOON (KR)
KIM SUNGSOO (KR)
LEE SANG KYU (KR)
KYUNG TAEYOON (KR)
Application Number:
PCT/KR2019/015459
Publication Date:
May 22, 2020
Filing Date:
November 13, 2019
Export Citation:
Assignee:
INST BASIC SCIENCE (KR)
International Classes:
C12N15/86; A01K67/027; C07K14/47; C07K14/705
Foreign References:
| KR20140031484A | 2014-03-13 |
Other References:
TAEYOON KYUNG ET AL: "Optogenetic control of endogenous Ca2+ channels in vivo.", NATURE BIOTECHNOLOGY, vol. 33, no. 10, 1 October 2015 (2015-10-01), pages 1092 - 1096, XP055707859, ISSN: 1087-0156, DOI: 10.1038/nbt.3350
DAPHNE CHE ET AL: "The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells", ACS SYNTHETIC BIOLOGY, vol. 4, no. 10, 18 May 2015 (2015-05-18), pages 1124 - 1135, XP055707864, ISSN: 2161-5063, DOI: 10.1021/acssynbio.5b00048
AMIR TASLIMI ET AL: "An optimized optogenetic clustering tool for probing protein interaction and function", NATURE COMMUNICATIONS, vol. 5, 4925, 18 September 2014 (2014-09-18), pages 1 - 18, XP055543041, DOI: 10.1038/ncomms5925
GOPAL PATHAK ET AL: "Benchmarking of optical dimerizer systems", ACS SYNTHETIC BIOLOGY, vol. 3, no. 11, 28 October 2014 (2014-10-28), pages 832 - 838, XP055707871, ISSN: 2161-5063, DOI: 10.1021/sb500291r
DAPHNE CHE ET AL: "The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells", ACS SYNTHETIC BIOLOGY, vol. 4, no. 10, 18 May 2015 (2015-05-18), pages 1124 - 1135, XP055707864, ISSN: 2161-5063, DOI: 10.1021/acssynbio.5b00048
AMIR TASLIMI ET AL: "An optimized optogenetic clustering tool for probing protein interaction and function", NATURE COMMUNICATIONS, vol. 5, 4925, 18 September 2014 (2014-09-18), pages 1 - 18, XP055543041, DOI: 10.1038/ncomms5925
GOPAL PATHAK ET AL: "Benchmarking of optical dimerizer systems", ACS SYNTHETIC BIOLOGY, vol. 3, no. 11, 28 October 2014 (2014-10-28), pages 832 - 838, XP055707871, ISSN: 2161-5063, DOI: 10.1021/sb500291r
Attorney, Agent or Firm:
YOU ME PATENT AND LAW FIRM (KR)
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