Title:
CRYOPRESERVATION SOLUTION WITHOUT DMSO, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/207151
Kind Code:
A1
Abstract:
Disclosed by the present invention is a cryopreservation liquid without DMSO. On the basis of a volume of every 100 mL, the cryopreservation solution contains 0.01-50.0 g of bionic ice control materials, 5.0-30 mL of polyols, 1-30 g of water-soluble sugar, and 0-30 mL of serum, the rest being a buffer solution. The bionic ice control materials are selected from PVA and/or amino acid bionic ice control materials. The cryopreservation solution according to the present invention and a freezing balancing solution do not contain DMSO. When being used for the cryopreservation of mouse oocytes and embryos, the described solution may achieve the same or an even higher cell and tissue survival rate and functional expression stability as or than a commercial cryopreservation solution (containing 15% DMSO), and has high preservation efficiency. The cryopreservation solution without DMSO or serum further solves the problems wherein parasitic biological contaminants may be introduced and stability is poor due to the commercial cryopreservation solution currently commonly used in clinical practice containing serum. The cryopreservation solution according to the present invention has a simple composition, a convenient source of raw materials and is low-cost, and may be widely applied in the cryopreservation of various types of cells.
Inventors:
YAN JIE (CN)
QIAO JIE (CN)
YAN LIYING (CN)
LI RONG (CN)
WANG JIANJUN (CN)
JIN SHENGLIN (CN)
LV JIANYONG (CN)
QIAO JIE (CN)
YAN LIYING (CN)
LI RONG (CN)
WANG JIANJUN (CN)
JIN SHENGLIN (CN)
LV JIANYONG (CN)
Application Number:
PCT/CN2020/077473
Publication Date:
October 15, 2020
Filing Date:
March 02, 2020
Export Citation:
Assignee:
UNIV PEKING THIRD HOSPITAL (CN)
INST CHEMISTRY CAS (CN)
INST CHEMISTRY CAS (CN)
International Classes:
A01N1/02; A01N1/00
Domestic Patent References:
| WO1991010361A1 | 1991-07-25 | |||
| WO2018191411A1 | 2018-10-18 |
Foreign References:
| CN108207930A | 2018-06-29 | |||
| CN101250499A | 2008-08-27 | |||
| CN109221082A | 2019-01-18 | |||
| CN201910281978A | 2019-04-09 | |||
| CN201910281986A | 2019-04-09 |
Other References:
黄凤玲 等 (HUANG, FENGLING ET AL.): "快速冷冻牛体外受精胚胎 (Non-official translation: Quick Freezing of Bovine IVF Embryos)", 广西农业大学学报 (JOURNAL OF GUANGXI AGRICULTURAL UNIVERSITY), vol. 13, no. 1, 30 March 1994 (1994-03-30), XP55742126, ISSN: 1005-2011, DOI: 20200410172547X
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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