Title:
CRYSTALLISED THERMOSTABLE GLYCOSYL HYDROLASE AND USE THEREOF FOR MODIFYING STRUCTURALLY RELATED ENZYMES
Document Type and Number:
WIPO Patent Application WO2003089633
Kind Code:
A3
Abstract:
The crystal of a hyperthermostable cellulase from Rhodothermus marinus and the three-dimensional structure of the enzyme are provided. The enzyme belongs to the family 12 glycosyl hydrolases. The invention further provides procedures for the identification of structural features that are important for thermostability of the enzyme. Methods based thereon to rationally modify proteins structurally related to R. marinus are disclosed, in particular, methods for increased thermostability are provided. Modified proteins are provided, including modified variants of cellulose from Trichoderma reesei.
Inventors:
CRENNELL SUSAN J (GB)
NORDBERG KARLSSON EVA MARGARET (SE)
HREGGVIDSSON GUDMUNDUR OLI (IS)
KRISTJANSSON JAKOB (IS)
AEVARSSON ARNTHOR (IS)
NORDBERG KARLSSON EVA MARGARET (SE)
HREGGVIDSSON GUDMUNDUR OLI (IS)
KRISTJANSSON JAKOB (IS)
AEVARSSON ARNTHOR (IS)
Application Number:
PCT/IS2003/000016
Publication Date:
April 08, 2004
Filing Date:
April 16, 2003
Export Citation:
Assignee:
PROKARIA EHF (IS)
CRENNELL SUSAN J (GB)
NORDBERG KARLSSON EVA MARGARET (SE)
HREGGVIDSSON GUDMUNDUR OLI (IS)
KRISTJANSSON JAKOB (IS)
AEVARSSON ARNTHOR (IS)
CRENNELL SUSAN J (GB)
NORDBERG KARLSSON EVA MARGARET (SE)
HREGGVIDSSON GUDMUNDUR OLI (IS)
KRISTJANSSON JAKOB (IS)
AEVARSSON ARNTHOR (IS)
International Classes:
C12N9/00; C12N9/24; C12N9/42; G06T17/00; G16B15/00; (IPC1-7): C12N9/42; G06T17/00
Domestic Patent References:
| WO2002012465A2 | 2002-02-14 | |||
| WO2001096382A2 | 2001-12-20 |
Other References:
CRENNELL SUSAN J ET AL: "The structure of Rhodothermus marinus Cel12A, a highly thermostable family 12 endoglucanase, at 1.8 ANG resolution.", JOURNAL OF MOLECULAR BIOLOGY, vol. 320, no. 4, 2002, 19 July, 2002, pages 883 - 897, XP002253705, ISSN: 0022-2836
SANCHEZ R ET AL: "Advances in comparative protein-structure modelling", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 7, 1997, pages 206 - 214, XP002190862, ISSN: 0959-440X
SANDGREN MATS ET AL: "The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12a, at 1.9 ANG resolution", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 308, no. 2, 2001, pages 295 - 310, XP002204008, ISSN: 0022-2836
SULZENBACHER GERLIND ET AL: "The Streptomyces lividans family 12 endoglucanase: Construction of the catalytic core, expression, and X-ray structure at 1.75 A resolution.", BIOCHEMISTRY, vol. 36, no. 51, 23 December 1997 (1997-12-23), pages 16032 - 16039, XP002253706, ISSN: 0006-2960
SZILAGYI ANDRAS ET AL: "Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: Results of a comprehensive survey.", STRUCTURE (LONDON), vol. 8, no. 5, 15 May 2000 (2000-05-15), pages 493 - 504, XP002253707, ISSN: 0969-2126
SANCHEZ-RUIZ JOSE M ET AL: "To charge or not to charge?", TRENDS IN BIOTECHNOLOGY, vol. 19, no. 4, April 2001 (2001-04-01), pages 132 - 135, XP002253708, ISSN: 0167-7799
CHI Y-I ET AL: "Crystal structure of the beta-glycosidase from the hyperthermophile Thermosphaera aggregans: insights into its activity and thermostability", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 445, no. 2-3, 26 February 1999 (1999-02-26), pages 375 - 383, XP004259293, ISSN: 0014-5793
HALLDORSDOTTIR S ET AL: "CLONING, SEQUENCING AND OVEREXPRESSION OF A RHODOTHERMUS MARINUS GENE ENCODING A THERMOSTABLE CELLULASE OF GLYCOSYL HYDROLASE FAMILY 12", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 49, no. 49, 1998, pages 277 - 284, XP002902257, ISSN: 0175-7598
SANCHEZ R ET AL: "Advances in comparative protein-structure modelling", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 7, 1997, pages 206 - 214, XP002190862, ISSN: 0959-440X
SANDGREN MATS ET AL: "The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12a, at 1.9 ANG resolution", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 308, no. 2, 2001, pages 295 - 310, XP002204008, ISSN: 0022-2836
SULZENBACHER GERLIND ET AL: "The Streptomyces lividans family 12 endoglucanase: Construction of the catalytic core, expression, and X-ray structure at 1.75 A resolution.", BIOCHEMISTRY, vol. 36, no. 51, 23 December 1997 (1997-12-23), pages 16032 - 16039, XP002253706, ISSN: 0006-2960
SZILAGYI ANDRAS ET AL: "Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: Results of a comprehensive survey.", STRUCTURE (LONDON), vol. 8, no. 5, 15 May 2000 (2000-05-15), pages 493 - 504, XP002253707, ISSN: 0969-2126
SANCHEZ-RUIZ JOSE M ET AL: "To charge or not to charge?", TRENDS IN BIOTECHNOLOGY, vol. 19, no. 4, April 2001 (2001-04-01), pages 132 - 135, XP002253708, ISSN: 0167-7799
CHI Y-I ET AL: "Crystal structure of the beta-glycosidase from the hyperthermophile Thermosphaera aggregans: insights into its activity and thermostability", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 445, no. 2-3, 26 February 1999 (1999-02-26), pages 375 - 383, XP004259293, ISSN: 0014-5793
HALLDORSDOTTIR S ET AL: "CLONING, SEQUENCING AND OVEREXPRESSION OF A RHODOTHERMUS MARINUS GENE ENCODING A THERMOSTABLE CELLULASE OF GLYCOSYL HYDROLASE FAMILY 12", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 49, no. 49, 1998, pages 277 - 284, XP002902257, ISSN: 0175-7598
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