Title:
CULTURE MEDIUM COMPOSITION FOR AMPLIFYING AND MAINTAINING SELF-RENEWAL CAPACITY AND DIFFERENTIATION POTENTIAL OF HSCS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/143675
Kind Code:
A1
Abstract:
A culture medium composition for amplifying and maintaining self-renewal capacity and differentiation potential of hematopoietic stem cells (HSCs), a cell population and an application thereof. The culture medium composition comprises a small molecule inhibitor of a hematopoietic stem cell culture medium and a PDGFR target. The inhibitor of PDGFR can significantly amplify HSCs during in-vitro culture, while maintaining a high proportion of self-renewal capacity of HSCs, which can achieve in-vitro amplification of HSCs while maintaining a higher proportion of dryness.
Inventors:
FANG RIGUO (CN)
SHI ZHONGYU (CN)
YANG HUIHUI (CN)
YUAN PENGFEI (CN)
SHI ZHONGYU (CN)
YANG HUIHUI (CN)
YUAN PENGFEI (CN)
Application Number:
PCT/CN2021/142127
Publication Date:
July 07, 2022
Filing Date:
December 28, 2021
Export Citation:
Assignee:
EDIGENE GUANGZHOU INC (CN)
EDIGENE INC (CN)
EDIGENE INC (CN)
International Classes:
C12N5/074; A61K35/28; A61P7/06
Foreign References:
| CN110461876A | 2019-11-15 | |||
| CN105713879A | 2016-06-29 | |||
| CN110713979A | 2020-01-21 |
Other References:
A. E. BOITANO, L. DE LICHTERVELDE, J. L. SNEAD, M. P. COOKE, P. G. SCHULTZ: "An image-based screen identifies a small molecule regulator of megakaryopoiesis", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 109, no. 35, 28 August 2012 (2012-08-28), pages 14019 - 14023, XP055122887, ISSN: 00278424, DOI: 10.1073/pnas.1212545109
LIM SUNG-EUN; ESAIN VIRGINIE; KWAN WANDA; THEODORE LINDSAY N.; CORTES MAURICIO; FROST ISAURA M.; LIU SARAH Y.; NORTH TRISTA E.: "HIF1α-induced PDGFRβ signaling promotes developmental HSC production via IL-6 activation", EXPERIMENTAL HEMATALOGY, vol. 46, 15 October 2016 (2016-10-15), US , pages 83 - 95+6, XP029883100, ISSN: 0301-472X, DOI: 10.1016/j.exphem.2016.10.002
LIM SUNG-EUN; ESAIN VIRGINIE; KWAN WANDA; THEODORE LINDSAY N.; CORTES MAURICIO; FROST ISAURA M.; LIU SARAH Y.; NORTH TRISTA E.: "HIF1α-induced PDGFRβ signaling promotes developmental HSC production via IL-6 activation", EXPERIMENTAL HEMATALOGY, vol. 46, 15 October 2016 (2016-10-15), US , pages 83 - 95+6, XP029883100, ISSN: 0301-472X, DOI: 10.1016/j.exphem.2016.10.002
Attorney, Agent or Firm:
BEIJING CAI HE LAW FIRM (CN)
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