Title:
CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS.
Document Type and Number:
WIPO Patent Application WO/2010/090513
Kind Code:
A3
Abstract:
The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.
Inventors:
SATO TOSHIRO (NL)
CLEVENS JOHANNES CAROLUS (NL)
HUCH ORTEGA MERITXELL (NL)
CLEVENS JOHANNES CAROLUS (NL)
HUCH ORTEGA MERITXELL (NL)
Application Number:
PCT/NL2010/000017
Publication Date:
November 25, 2010
Filing Date:
February 03, 2010
Export Citation:
Assignee:
KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN (NL)
SATO TOSHIRO (NL)
CLEVENS JOHANNES CAROLUS (NL)
HUCH ORTEGA MERITXELL (NL)
SATO TOSHIRO (NL)
CLEVENS JOHANNES CAROLUS (NL)
HUCH ORTEGA MERITXELL (NL)
International Classes:
C12N5/073
Domestic Patent References:
| WO2008101215A1 | 2008-08-21 |
Other References:
ABUD H E ET AL: "Growth of intestinal epithelium in organ culture is dependent on EGF signalling", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, vol. 303, no. 2, 15 February 2005 (2005-02-15), pages 252 - 262, XP004709265, ISSN: 0014-4827
BJERKNES MATTHEW ET AL: "Intestinal epithelial stem cells and progenitors", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 419, 1 January 2006 (2006-01-01), pages 337 - 383, XP009101698, ISSN: 0076-6879
BOOTH C ET AL: "Maintenance of functional stem cells in isolated and cultured adult intestinal epithelium", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, vol. 249, no. 2, 15 June 1999 (1999-06-15), pages 359 - 366, XP002232585, ISSN: 0014-4827
HARAMIS ANNA-PAVLINA G ET AL: "De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine.", SCIENCE (WASHINGTON D C), vol. 303, no. 5664, 12 March 2004 (2004-03-12), pages 1684 - 1686, XP002529276, ISSN: 0036-8075
KIM K-A ET AL: "Mitogenic Influence of Human R-Spondin1 on the Intestinal Epithelium", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 309, 19 August 2005 (2005-08-19), pages 1256 - 1259, XP003014398, ISSN: 0036-8075
BARKER N ET AL: "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, NATURE PUBLISHING GROUP, LONDON, GB LNKD- DOI:10.1038/NATURE06196, vol. 449, 25 October 2007 (2007-10-25), pages 1003 - 1008, XP002457164, ISSN: 0028-0836
SATO TOSHIRO ET AL: "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.", NATURE 14 MAY 2009, vol. 459, no. 7244, 14 May 2009 (2009-05-14), pages 262 - 265, XP002529277, ISSN: 1476-4687
BJERKNES MATTHEW ET AL: "Intestinal epithelial stem cells and progenitors", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 419, 1 January 2006 (2006-01-01), pages 337 - 383, XP009101698, ISSN: 0076-6879
BOOTH C ET AL: "Maintenance of functional stem cells in isolated and cultured adult intestinal epithelium", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, vol. 249, no. 2, 15 June 1999 (1999-06-15), pages 359 - 366, XP002232585, ISSN: 0014-4827
HARAMIS ANNA-PAVLINA G ET AL: "De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine.", SCIENCE (WASHINGTON D C), vol. 303, no. 5664, 12 March 2004 (2004-03-12), pages 1684 - 1686, XP002529276, ISSN: 0036-8075
KIM K-A ET AL: "Mitogenic Influence of Human R-Spondin1 on the Intestinal Epithelium", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 309, 19 August 2005 (2005-08-19), pages 1256 - 1259, XP003014398, ISSN: 0036-8075
BARKER N ET AL: "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, NATURE PUBLISHING GROUP, LONDON, GB LNKD- DOI:10.1038/NATURE06196, vol. 449, 25 October 2007 (2007-10-25), pages 1003 - 1008, XP002457164, ISSN: 0028-0836
SATO TOSHIRO ET AL: "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.", NATURE 14 MAY 2009, vol. 459, no. 7244, 14 May 2009 (2009-05-14), pages 262 - 265, XP002529277, ISSN: 1476-4687
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