Title:
CULTURE MEDIUM WITH TWO LAYERS FOR DETECTING MICROORGANISMS
Document Type and Number:
WIPO Patent Application WO/2017/138046
Kind Code:
A1
Abstract:
To provide a method in which staining of a culture medium itself can be suppressed even if an enzyme that can decompose an enzyme substrate to be decomposed by an enzyme of microorganisms inherently exists in an analyte, and target microorganisms can be detected as stained colony with high accuracy, and an enzyme substrate culture medium therefor. A culture medium for detecting microorganisms includes a first layer in which a gelling agent and an enzyme substrate are contained, and a second layer in which a gelling agent is contained and the enzyme substrate is not contained to be laminated adjacently to the first layer, wherein the enzyme substrate is a compound from which a dye compound can be released.
Inventors:
TERAMURA HAJIME (JP)
IWASAKI MIHOKO (JP)
SOTA KOJIRO (JP)
IWASAKI MIHOKO (JP)
SOTA KOJIRO (JP)
Application Number:
PCT/JP2016/004455
Publication Date:
August 17, 2017
Filing Date:
October 03, 2016
Export Citation:
Assignee:
JNC CORP (JP)
International Classes:
C12Q1/04; C12M1/16
Domestic Patent References:
| WO2015134686A1 | 2015-09-11 | |||
| WO1997024432A1 | 1997-07-10 |
Foreign References:
| EP0928830A1 | 1999-07-14 | |||
| EP0877081A1 | 1998-11-11 | |||
| JP2002537852A | 2002-11-12 | |||
| JPH10501129A | 1998-02-03 | |||
| JPH0919282A | 1997-01-21 | |||
| JP2015204845A | 2015-11-19 |
Other References:
GUICHARD H ET AL: "Development and validation of a plate technique for screening of microorganisms that produce volatile sulfur compounds", ANALYTICAL BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 338, no. 2, 15 March 2005 (2005-03-15), pages 299 - 305, XP004770831, ISSN: 0003-2697, DOI: 10.1016/J.AB.2004.12.027
LIMUTI C M ET AL: "Colorimetric detection of microbially excreted lysine directly on agar plates", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 9, no. 2, 1 February 1989 (1989-02-01), pages 129 - 137, XP023699253, ISSN: 0167-7012, [retrieved on 19890201], DOI: 10.1016/0167-7012(89)90063-8
LIMUTI C M ET AL: "Colorimetric detection of microbially excreted lysine directly on agar plates", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 9, no. 2, 1 February 1989 (1989-02-01), pages 129 - 137, XP023699253, ISSN: 0167-7012, [retrieved on 19890201], DOI: 10.1016/0167-7012(89)90063-8
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
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