CULTURE METHOD AND CELL MASS

Title:

CULTURE METHOD AND CELL MASS

Document Type and Number:

WIPO Patent Application WO/2015/182159

Kind Code:

A1

Abstract:

　The present invention is a culture method for culturing a population consisting of two or more cells including stem-cell-derived cells and mesenchymal cells in an indentation (10). Stem-cell-derived cells are cells obtained by differentiating stem cells in vitro. Such cells are one or more types of cell selected from the group consisting of endodermal cells, ectodermal cells, and mesodermal cells. The population is cultured in an indentation (10) together with vascular cells or secretory components. The indentation (10) has a space in which the cells can move. The volume of the space is taken to be V mm3. The number of mesenchymal cells seeded in the space is taken to be N. At this time, V is 400 or less. Furthermore, N/V is from 35 to 3000.

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Inventors:

EJIRI YOKO (JP)
TANIGUCHI HIDEKI (JP)
TAKEBE TAKANORI (JP)

Application Number:

PCT/JP2015/002738

Publication Date:

December 03, 2015

Filing Date:

May 29, 2015

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Assignee:

KURARAY CO (JP)
PUBLIC UNIV CORP YOKOHAMA CITY (JP)

International Classes:

C12N5/10; C12N1/00; C12N5/0735; C12N5/077

Domestic Patent References:

WO2013047639A1	2013-04-04
WO2008130025A1	2008-10-30
WO2013042360A1	2013-03-28
WO2012036011A1	2012-03-22

Foreign References:

JP2001509272A	2001-07-10
JP2010088347A	2010-04-22

Other References: