Title:
CULTURE METHOD, GROUP OF MATURE ADIPOCYTES, AND DRUG SCREENING METHOD
Document Type and Number:
WIPO Patent Application WO/2012/124353
Kind Code:
A1
Abstract:
In an adipocyte culture method, seeded adipocytes are cultured while adhering the adipocytes to a culture bottom surface (14) and walls (12) positioned perpendicularly to the culture bottom surface (14), and mature adipocytes are obtained by generating spherical, enlarged fat droplets in cells without suspending the adipocytes in a culture medium. In the method, a culture vessel (10) is used in which the culture walls (12) are formed perpendicularly to the culture bottom surface (14). With cells or cell aggregates adhered in at least two places, namely the walls (12) and the culture bottom surface (14), spherical mature adipocytes having spherical enlarged fat droplets in cells are cultured. Adipocytes with a form close to in vivo adipocytes are thus obtained.
Inventors:
ITCHODA YOKO (JP)
HOSODA MASAYA (JP)
FUKUDA MOTOHIRO (JP)
NAGAYAMA MASAFUMI (JP)
HOSODA MASAYA (JP)
FUKUDA MOTOHIRO (JP)
NAGAYAMA MASAFUMI (JP)
Application Number:
PCT/JP2012/001872
Publication Date:
September 20, 2012
Filing Date:
March 16, 2012
Export Citation:
Assignee:
KURARAY CO (JP)
UNIV HOKKAIDO NAT UNIV CORP (JP)
ITCHODA YOKO (JP)
HOSODA MASAYA (JP)
FUKUDA MOTOHIRO (JP)
NAGAYAMA MASAFUMI (JP)
UNIV HOKKAIDO NAT UNIV CORP (JP)
ITCHODA YOKO (JP)
HOSODA MASAYA (JP)
FUKUDA MOTOHIRO (JP)
NAGAYAMA MASAFUMI (JP)
International Classes:
C12N5/077; C12Q1/02; C12M3/00
Domestic Patent References:
| WO2007125859A1 | 2007-11-08 |
Foreign References:
| JP2000083656A | 2000-03-28 |
Other References:
YOKO EJIRI ET AL.: "Soyaku Screening Yoto o Mezashita Shinki Baiyo Kizai no Kaihatsu", DAI 24 KAI THE JAPANESE SOCIETY FOR THE STUDY OF XENOBIOTICS NENKAI KOEN YOSHISHU, 27 November 2009 (2009-11-27), pages 172, XP008170431
MASAFUMI NAGAYAMA ET AL.: "Shibo Saibo eno Bunka Katei ni Okeru Saibo Oyobi Shiboteki no Kyodo - Time Lapse Kansatsu ni yoru - Saibo Level deno Kaimei", JOURNAL OF JAPAN SOCIETY FOR THE STUDY OF OBESITY, vol. 13, no. 1, 2007, pages 84 - 86, XP008170430
NAGAYAMA, M. ET AL.: "Temporal and spatial variations of lipid droplets during adipocyte division and differentiation", THE JOURNAL OF LIPID RESEARCH, vol. 48, no. 1, 2007, pages 9 - 18, XP055120567
See also references of EP 2687594A4
RACHEL GLICKLIS ET AL.: "Modeling Mass Transfer in Hepatocyte Spheroids via Cell Viability, Spheroid Size, and Hepatocellualr Functions", 23 April 2004, WILEY INTERSCIENCE
FRANZISKA HIRSCHHAEUSER ET AL.: "Multicelluar tumor spheroids: An underestimated tool is catching up again", JOURNAL OF BIOTECHNOLOGY, vol. 148, 2010, pages 3 - 15
MASAFUMI NAGAYAMA ET AL.: "Shibo Saibo eno Bunka Katei ni Okeru Saibo Oyobi Shiboteki no Kyodo - Time Lapse Kansatsu ni yoru - Saibo Level deno Kaimei", JOURNAL OF JAPAN SOCIETY FOR THE STUDY OF OBESITY, vol. 13, no. 1, 2007, pages 84 - 86, XP008170430
NAGAYAMA, M. ET AL.: "Temporal and spatial variations of lipid droplets during adipocyte division and differentiation", THE JOURNAL OF LIPID RESEARCH, vol. 48, no. 1, 2007, pages 9 - 18, XP055120567
See also references of EP 2687594A4
RACHEL GLICKLIS ET AL.: "Modeling Mass Transfer in Hepatocyte Spheroids via Cell Viability, Spheroid Size, and Hepatocellualr Functions", 23 April 2004, WILEY INTERSCIENCE
FRANZISKA HIRSCHHAEUSER ET AL.: "Multicelluar tumor spheroids: An underestimated tool is catching up again", JOURNAL OF BIOTECHNOLOGY, vol. 148, 2010, pages 3 - 15
Attorney, Agent or Firm:
IEIRI, Takeshi (JP)
House ON 健 (JP)
House ON 健 (JP)
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Claims: