Title:
CULTURED CELLS AND SUGAR SOLUTION PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/192647
Kind Code:
A1
Abstract:
Provided is an enzyme solution in which the saccharification rate is not prone to decrease in saccharification reactions that extend over a long period of time. Trichoderma fungal culture cells which express β-glucosidase derived from Pyrococcus furiosus are used in the saccharification reaction. Further, by expressing in Trichoderma fungal culture cells the genes of the β-glucosidase derived from Pyrococcus furiosus in which codons have been changed, the expressed β-glucosidase is accumulated and retained within the cells without being immediately secreted.
Inventors:
ISHIKAWA KAZUHIKO (JP)
OGASAWARA WATARU (JP)
SHIDA YOSUKE (JP)
HIRAMATSU SHINGO (JP)
KURIHARA HIROYUKI (JP)
YAMADA KATSUSHIGE (JP)
OGASAWARA WATARU (JP)
SHIDA YOSUKE (JP)
HIRAMATSU SHINGO (JP)
KURIHARA HIROYUKI (JP)
YAMADA KATSUSHIGE (JP)
Application Number:
PCT/JP2014/063656
Publication Date:
December 04, 2014
Filing Date:
May 23, 2014
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
NAT UNIV CORP NAGAOKA UNIV TEC (JP)
TORAY INDUSTRIES (JP)
NAT UNIV CORP NAGAOKA UNIV TEC (JP)
TORAY INDUSTRIES (JP)
International Classes:
C12N15/09; C12N1/15; C12P19/02
Domestic Patent References:
| WO2011021616A1 | 2011-02-24 |
Foreign References:
| JP2010516296A | 2010-05-20 | |||
| JP2012034690A | 2012-02-23 | |||
| JP2012235767A | 2012-12-06 | |||
| JP2012016329A | 2012-01-26 | |||
| JP2010528655A | 2010-08-26 |
Other References:
TE'O V. S. J. ET AL.: "Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei.", FEMS MICROBIOLOGY LETTERS, vol. 190, 2000, pages 13 - 19, XP002423247, DOI: doi:10.1111/j.1574-6968.2000.tb09255.x
WANPING CHEN ET AL.: "Genomic characteristics comparisons of 12 food-related filamentous fungi in tRNA gene set, codon usage and amino acid composition", GENE, vol. 497, 2012, pages 116 - 124, XP028464749, DOI: doi:10.1016/j.gene.2012.01.016
PETER BERGQUIST ET AL.: "Expression of xylanase enzymes from thermophilic microorganisms in fungal hosts", EXTREMOPHILES, vol. 6, 2002, pages 177 - 184, XP002327848, DOI: doi:10.1007/s00792-001-0252-5
YOSUKE SHIDA ET AL.: "Expression of the extremely thermostable beta-glucosidase in the filamentous fungus Trichoderma reesei", ABSTRACTS OF THE 65TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 August 2013 (2013-08-25), JAPAN, pages 64
WANPING CHEN ET AL.: "Genomic characteristics comparisons of 12 food-related filamentous fungi in tRNA gene set, codon usage and amino acid composition", GENE, vol. 497, 2012, pages 116 - 124, XP028464749, DOI: doi:10.1016/j.gene.2012.01.016
PETER BERGQUIST ET AL.: "Expression of xylanase enzymes from thermophilic microorganisms in fungal hosts", EXTREMOPHILES, vol. 6, 2002, pages 177 - 184, XP002327848, DOI: doi:10.1007/s00792-001-0252-5
YOSUKE SHIDA ET AL.: "Expression of the extremely thermostable beta-glucosidase in the filamentous fungus Trichoderma reesei", ABSTRACTS OF THE 65TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 August 2013 (2013-08-25), JAPAN, pages 64
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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