Title:
CURCULIN B, DNA CODING FOR THE SAME, AND PRODUCTION OF BOTH OF THEM
Document Type and Number:
WIPO Patent Application WO/1992/015612
Kind Code:
A1
Abstract:
A process for producing curculin B, which comprises culturing a transformed microorganism or cell containing inherently pure curculin B or a recombinant DNA containing a base sequence which codes for curculin B to thereby produce curculin B and isolating the produced curculin B from the transformed microorganism or cell. A process for producing a DNA containing a base sequence coding for curculin B, which comprises isolating a fraction containing mRNA of curculin B from a DNA containing a base sequence coding for curculin B or its precursor or from Curculigo latifolia, preparing a single-stranded cDNA from the mRNA with a reverse transcriptase, preparing a double-stranded cDNA from the single-stranded cDNA, inserting the double-stranded cDNA into a vector, transforming the host with the resultant vector to prepare a cDNA library, and isolating a cDNA coding for curculin B from this library by using a probe comprising a synthetic DNA containing a base sequence coding for a partial amino acid sequence obtained by analyzing purified curculin A obtained from Curculigo latifolia.
Inventors:
KURIHARA YOSHIE (JP)
ARAI SOICHI (JP)
ABE KEIKO (JP)
YAMASHITA HARUYUKI (JP)
ARAI SOICHI (JP)
ABE KEIKO (JP)
YAMASHITA HARUYUKI (JP)
Application Number:
PCT/JP1992/000165
Publication Date:
September 17, 1992
Filing Date:
February 19, 1992
Export Citation:
Assignee:
KURIHARA YOSHIE (JP)
ARAI SOICHI (JP)
ASAHI DENKA KOGYO KK (JP)
ARAI SOICHI (JP)
ASAHI DENKA KOGYO KK (JP)
International Classes:
A23L27/21; A23L27/30; C07K14/43; C12N15/29; C12P21/02; C07K14/245; A61K38/00; (IPC1-7): A23L1/22; C07K13/00; C07K15/04; C07K15/10; C12N15/29; C12P21/02
Foreign References:
| JPH02104263A | 1990-04-17 | |||
| JPH03190899A | 1991-08-20 |
Other References:
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 265, No. 26, (1990), H. YAMASHITA et al., "Purification and Complete Amino Acid Sequence of a New Type of Sweet Protein With Taste-Modifying Activity, Curculin", pages 15770-15775.
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