Title:
CURCUMA WENYUJIN Y. H. CHEN & C. LING-DERIVED CURCUMIN SYNTHETASE, GENE, VECTOR, ENGINEERED BACTERIUM, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/202122
Kind Code:
A1
Abstract:
Provided are a Curcuma wenyujin Y. H. Chen & C. Ling-derived curcumin synthetase, a gene, a vector, an engineered bacterium, and use thereof. The amino acid sequence of the curcumin synthetase is set forth in SEQ. ID NO. 2. The sequence of the coding gene of said curcumin synthetase is set forth in SEQ. ID NO. 1. The constructed recombinant vector is pYES2::CwPKS. The recombinant genetically engineered bacterium is Saccharomyces cerevisiae BY4741/pESC::LEU::At4CL-CIDCS/pYES2::CwPKS. The genetically engineered bacterium has the ability to produce curcumin using ferulic acid, produce mono-demethoxycurcumin using ferulic acid and coumaric acid, and produce tetrahydrobisdemethoxycurcumin using dihydrocoumaric acid. A catalyst can be quickly and massively prepared in yeast cells for use in large-scale synthesis of curcumin compounds.
Inventors:
XIE TIAN (CN)
CHEN RONG (CN)
YIN XIAOPU (CN)
HU TIANYUAN (CN)
WANG MING (CN)
CHEN SHU (CN)
HU YUHAN (CN)
CHEN RONG (CN)
YIN XIAOPU (CN)
HU TIANYUAN (CN)
WANG MING (CN)
CHEN SHU (CN)
HU YUHAN (CN)
Application Number:
PCT/CN2022/140230
Publication Date:
October 26, 2023
Filing Date:
December 20, 2022
Export Citation:
Assignee:
UNIV HANGZHOU NORMAL (CN)
International Classes:
C12N9/10; C12N1/18; C12N15/81; C12P7/26; C12R1/865
Foreign References:
| CN114591929A | 2022-06-07 | |||
| CN106497904A | 2017-03-15 | |||
| CN106987566A | 2017-07-28 | |||
| JP2010200630A | 2010-09-16 |
Other References:
DATABASE Nucleotide 15 October 2017 (2017-10-15), ANONYMOUS: "Curcuma longa type III polyketide synthase (pks11) mRNA, complete cds", XP093098158, retrieved from NCBI Database accession no. KX017475.1
RAINHA JOÃO, RODRIGUES JOANA L., FARIA CRISTIANA, RODRIGUES LÍGIA R.: "Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 17, no. 3, 1 March 2022 (2022-03-01), DE , pages :2100400, XP093098132, ISSN: 1860-6768, DOI: 10.1002/biot.202100400
CLAIRE M. PALMER; HAL S. ALPER: "Expanding the Chemical Palette of Industrial Microbes: Metabolic Engineering for Type III PKS‐Derived Polyketides", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 14, no. 1, 19 November 2018 (2018-11-19), DE , pages n/a - n/a, XP072422469, ISSN: 1860-6768, DOI: 10.1002/biot.201700463
CHEN RONG, HU TIANYUAN, WANG MING, HU YUHAN, CHEN SHU, WEI QIUHUI, YIN XIAOPU, XIE TIAN: "Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis in Curcuma wenyujin", SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, ELSEVIER, CN, NL, vol. 7, no. 3, 1 September 2022 (2022-09-01), CN, NL , pages 849 - 861, XP093098133, ISSN: 2405-805X, DOI: 10.1016/j.synbio.2022.04.006
RAINHA JOÃO, RODRIGUES JOANA L., FARIA CRISTIANA, RODRIGUES LÍGIA R.: "Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 17, no. 3, 1 March 2022 (2022-03-01), DE , pages :2100400, XP093098132, ISSN: 1860-6768, DOI: 10.1002/biot.202100400
CLAIRE M. PALMER; HAL S. ALPER: "Expanding the Chemical Palette of Industrial Microbes: Metabolic Engineering for Type III PKS‐Derived Polyketides", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 14, no. 1, 19 November 2018 (2018-11-19), DE , pages n/a - n/a, XP072422469, ISSN: 1860-6768, DOI: 10.1002/biot.201700463
CHEN RONG, HU TIANYUAN, WANG MING, HU YUHAN, CHEN SHU, WEI QIUHUI, YIN XIAOPU, XIE TIAN: "Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis in Curcuma wenyujin", SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, ELSEVIER, CN, NL, vol. 7, no. 3, 1 September 2022 (2022-09-01), CN, NL , pages 849 - 861, XP093098133, ISSN: 2405-805X, DOI: 10.1016/j.synbio.2022.04.006
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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