Title:
A CYANINE-BASED PROBE\TAG-PEPTIDE PAIR FOR FLUORESCENCE PROTEIN IMAGING AND FLUORESCENCE PROTEIN IMAGING METHODS
Document Type and Number:
WIPO Patent Application WO2008127768
Kind Code:
B1
Abstract:
A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCI3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.
Inventors:
MAYER-CUMBLIDGE ULJANA M (US)
CAO HAISHI (US)
CAO HAISHI (US)
Application Number:
PCT/US2008/053600
Publication Date:
December 11, 2008
Filing Date:
February 11, 2008
Export Citation:
Assignee:
BATTELLE MEMORIAL INSTITUTE (US)
MAYER-CUMBLIDGE ULJANA M (US)
CAO HAISHI (US)
MAYER-CUMBLIDGE ULJANA M (US)
CAO HAISHI (US)
International Classes:
C09B23/06; C07K1/13; C09B23/08
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