Title:
CYSTEINE ENGINEERED ANTIBODIES AND CONJUGATES
Document Type and Number:
WIPO Patent Application WO/2016/040856
Kind Code:
A3
Abstract:
Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody.
Inventors:
BHAKTA SUNIL (US)
ERICKSON HANS (US)
JUNUTULA JAGATH R (US)
KOZAK KATHERINE (US)
OHRI RACHANA (US)
PILLOW THOMAS (US)
ERICKSON HANS (US)
JUNUTULA JAGATH R (US)
KOZAK KATHERINE (US)
OHRI RACHANA (US)
PILLOW THOMAS (US)
Application Number:
PCT/US2015/049771
Publication Date:
May 06, 2016
Filing Date:
September 11, 2015
Export Citation:
Assignee:
GENENTECH INC (US)
HOFFMANN LA ROCHE (CH)
HOFFMANN LA ROCHE (CH)
International Classes:
A61K47/48; C07K16/28; A61P35/00; C07K16/18; C07K16/30; C07K16/32
Domestic Patent References:
| WO2014124316A2 | 2014-08-14 | |||
| WO2013093809A1 | 2013-06-27 | |||
| WO2012074757A1 | 2012-06-07 | |||
| WO2011156328A1 | 2011-12-15 | |||
| WO2010108937A2 | 2010-09-30 |
Foreign References:
| US20120009621A1 | 2012-01-12 |
Other References:
JUNUTULA JAGATH REDDY ET AL: "ThioMabs: improving safety abd retaining efficacy of antibody drug conjugates", AMERICAN ASSOCIATION FOR CANCER RESEARCH. PROCEEDINGS OF THE ANNUAL MEETING, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 48, 1 April 2007 (2007-04-01), pages 220 - 221, XP001538787, ISSN: 0197-016X
BHAKTA SUNIL ET AL: "Engineering THIOMABs for Site-Specific Conjugation of Thiol-Reactive Linkers", ANTIBODY-DRUG CONJUGATES HUMANA PRESS INC, 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA SERIES : METHODS IN MOLECULAR BIOLOGY (ISSN 1064-3745(PRINT)), 2013, pages 189 - 203, XP008178420
JUNUTULA J R ET AL: "Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, US, vol. 26, no. 8, 1 August 2008 (2008-08-01), pages 925 - 932, XP002499771, ISSN: 1087-0156, [retrieved on 20080720], DOI: 10.1038/NBT.1480
C. ANDREW BOSWELL ET AL: "Impact of Drug Conjugation on Pharmacokinetics and Tissue Distribution of Anti-STEAP1 Antibody-Drug Conjugates in Rats", BIOCONJUGATE CHEMISTRY, vol. 22, no. 10, 19 October 2011 (2011-10-19), pages 1994 - 2004, XP055144339, ISSN: 1043-1802, DOI: 10.1021/bc200212a
SUKUMARAN SIDDHARTH ET AL: "Mechanism-Based Pharmacokinetic/Pharmacodynamic Model for THIOMAB(TM) Drug Conjug", PHARMACEUTICAL RESEARCH, SPRINGER NEW YORK LLC, US, vol. 32, no. 6, 2 December 2014 (2014-12-02), pages 1884 - 1893, XP035498426, ISSN: 0724-8741, [retrieved on 20141202], DOI: 10.1007/S11095-014-1582-1
BHAKTA SUNIL ET AL: "Engineering THIOMABs for Site-Specific Conjugation of Thiol-Reactive Linkers", ANTIBODY-DRUG CONJUGATES HUMANA PRESS INC, 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA SERIES : METHODS IN MOLECULAR BIOLOGY (ISSN 1064-3745(PRINT)), 2013, pages 189 - 203, XP008178420
JUNUTULA J R ET AL: "Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, US, vol. 26, no. 8, 1 August 2008 (2008-08-01), pages 925 - 932, XP002499771, ISSN: 1087-0156, [retrieved on 20080720], DOI: 10.1038/NBT.1480
C. ANDREW BOSWELL ET AL: "Impact of Drug Conjugation on Pharmacokinetics and Tissue Distribution of Anti-STEAP1 Antibody-Drug Conjugates in Rats", BIOCONJUGATE CHEMISTRY, vol. 22, no. 10, 19 October 2011 (2011-10-19), pages 1994 - 2004, XP055144339, ISSN: 1043-1802, DOI: 10.1021/bc200212a
SUKUMARAN SIDDHARTH ET AL: "Mechanism-Based Pharmacokinetic/Pharmacodynamic Model for THIOMAB(TM) Drug Conjug", PHARMACEUTICAL RESEARCH, SPRINGER NEW YORK LLC, US, vol. 32, no. 6, 2 December 2014 (2014-12-02), pages 1884 - 1893, XP035498426, ISSN: 0724-8741, [retrieved on 20141202], DOI: 10.1007/S11095-014-1582-1
Attorney, Agent or Firm:
SCARR, Rebecca B. et al. (PLLC500 W. Silver Springs Drive,Suite K-20, Glendale Wisconsin, US)
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