Title:
CYTOCHROME B5 FROM PETUNIA
Document Type and Number:
WIPO Patent Application WO2000009720
Kind Code:
A3
Abstract:
The present invention relates generally to a plant regulatory gene and derivatives and homologues thereof. More particularly, the present invention provides a nucleic acid molecule comprising a sequence of nucleotides or complementary sequence of nucleotides, the expression of which, modulates or otherwise facilitates activity of a cytochrome P450 protein in plant cells and tissues including petals, flowers, stems, leaves and seeds. Even more particularly, the nucleic acid molecule of the present invention encodes a cytochrome b5 or a mutant, part, fragment, portion, functional and/or structural equivalent or homologue thereof or agonist or antagonist thereof involved in modulating or otherwise facilitating activity of a dihydrokaempferol (DHK) hydroxylating enzyme such as but not limited to flavonoid 3', 5'-hydroxylase. The present invention further provides transgenic plants or parts thereof or cells of transgenic plants as well as cut or severed flowers or stems from transgenic plants.
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Inventors:
KOES RONALD (NL)
DE VETTEN NICK (NL)
MOL JOS (NL)
DE VETTEN NICK (NL)
MOL JOS (NL)
Application Number:
PCT/GB1999/002676
Publication Date:
May 18, 2000
Filing Date:
August 13, 1999
Export Citation:
Assignee:
INT FLOWER DEV PTY LTD (AU)
VRIJE UNIVERSITEIT (NL)
JONES ELIZABETH (GB)
KOES RONALD (NL)
VETTEN NICK DE (NL)
MOL JOS (NL)
VRIJE UNIVERSITEIT (NL)
JONES ELIZABETH (GB)
KOES RONALD (NL)
VETTEN NICK DE (NL)
MOL JOS (NL)
International Classes:
C07K14/80; C12N15/82; (IPC1-7): C12N15/82; C07K14/80; A01H5/00
Domestic Patent References:
| WO1993001290A1 | 1993-01-21 |
Other References:
SMITH M A ET AL.: "Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting", PLANT MOLECULAR BIOLOGY, vol. 25, no. 3, June 1994 (1994-06-01), pages 527 - 537, XP002129200
SAYANOVA O ET AL: "Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of hig levels of Delta6-desaturated fatty acids in transgenic tobacco", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, 15 April 1997 (1997-04-15), pages 4211 - 4216, XP002106758, ISSN: 0027-8424
KEARNS E V ET AL.: "Primary structure of cytochrome b5 from cauliflower (Brassica oleracea L.) deduced from peptide and cDNA sequences", PLANT PHYSIOOGY, vol. 99, 1992, pages 1254 - 1257, XP002129201
DE VETTEN N ET AL: "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 96, no. 2, January 1999 (1999-01-01), pages 778 - 783, XP002129202
MARTSINKOVSKAYA A I ET AL: "Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives", PLANT MOLECULAR BIOLOGY, vol. 40, no. 1, May 1999 (1999-05-01), pages 79 - 90, XP002129203
SAYANOVA O ET AL: "Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of hig levels of Delta6-desaturated fatty acids in transgenic tobacco", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, 15 April 1997 (1997-04-15), pages 4211 - 4216, XP002106758, ISSN: 0027-8424
KEARNS E V ET AL.: "Primary structure of cytochrome b5 from cauliflower (Brassica oleracea L.) deduced from peptide and cDNA sequences", PLANT PHYSIOOGY, vol. 99, 1992, pages 1254 - 1257, XP002129201
DE VETTEN N ET AL: "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 96, no. 2, January 1999 (1999-01-01), pages 778 - 783, XP002129202
MARTSINKOVSKAYA A I ET AL: "Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives", PLANT MOLECULAR BIOLOGY, vol. 40, no. 1, May 1999 (1999-05-01), pages 79 - 90, XP002129203
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