Title:
CYTOMETRIC MECHANISM, CELL CULTURE DEVICE COMPRISING SAME, AND CYTOMETRIC METHOD
Document Type and Number:
WIPO Patent Application WO/2016/063364
Kind Code:
A1
Abstract:
The present invention provides a cytometric mechanism, cell culture device, and cytometric method with which it is possible to measure a cell survival rate or the like rapidly and at high accuracy, in a manner not dependent on a worker's skill level and without having to stain cultured cells. A cytometric mechanism 16 comprises: a flow path through which a cell suspension is made to flow; a liquid drive unit 7 for sending the cell suspension which is in the flow path; and a computation unit 18 for irradiating, with irradiation light from a light source 22, a cell suspension flowing through a flow cell 23, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database 18d for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.
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Inventors:
KAWARAI MASAKO (JP)
SAKURAI TOSHINARI (JP)
SHIMASE AKIHIRO (JP)
KOSHI HIROYUKI (JP)
SAKURAI TOSHINARI (JP)
SHIMASE AKIHIRO (JP)
KOSHI HIROYUKI (JP)
Application Number:
PCT/JP2014/078033
Publication Date:
April 28, 2016
Filing Date:
October 22, 2014
Export Citation:
Assignee:
HITACHI HIGH TECH CORP (JP)
International Classes:
C12M1/34; G01N15/14; G01N21/49; G01N33/48; G01N33/483
Foreign References:
| JP2012143231A | 2012-08-02 | |||
| JPH02500297A | 1990-02-01 | |||
| JP2006220423A | 2006-08-24 | |||
| JPH03503808A | 1991-08-22 | |||
| JP2013522644A | 2013-06-13 | |||
| JP2012507008A | 2012-03-22 |
Attorney, Agent or Firm:
POLAIRE I. P. C. (JP)
Polaire Intellectual Property Corporation (JP)
Polaire Intellectual Property Corporation (JP)
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