Title:
D-AMINO ACID OXIDASE AND USE THEREOF IN PREPARATION OF L-PHOSPHINOTHRICIN OR INTERMEDIATE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/143621
Kind Code:
A1
Abstract:
Provided are a D-amino acid oxidase and use thereof in the preparation of L-phosphinothricin or an intermediate thereof. Provided is a D-amino acid oxidase having an amino acid sequence comprising an amino acid residue difference as compared to SEQ ID NO: 1, the amino acid residue difference being selected from one or a plurality of: K29G/H/I/N/Q/W/Y/C/L; V42C/D/E/H/Y; E195N/Y/Q; C234L; and V326W. The activity and/or thermal stability of the D-amino acid oxidase is not lower than that of a D-amino acid oxidase having an amino acid sequence as set forth in SEQ ID NO: 1. Provided is a D-amino acid oxidase with higher thermal stability. The operating temperature range of the enzyme is expanded while the activity of the enzyme is improved. The enzyme can have a prolonged service life when used at a relatively low temperature, and can have an improved catalytic efficiency when used at a relatively high temperature.
Inventors:
JIAO QI (CN)
WANG SHU (CN)
TIAN ZHENHUA (CN)
CHENG ZHANBING (CN)
MA SHUAI (CN)
CHENG JING (CN)
WANG SHU (CN)
TIAN ZHENHUA (CN)
CHENG ZHANBING (CN)
MA SHUAI (CN)
CHENG JING (CN)
Application Number:
PCT/CN2023/073897
Publication Date:
August 03, 2023
Filing Date:
January 30, 2023
Export Citation:
Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/53; C12N9/06; C12N15/70; C12P9/00; C12P13/04
Domestic Patent References:
| WO2020103928A1 | 2020-05-28 | |||
| WO2020233451A1 | 2020-11-26 |
Foreign References:
| CN110055289A | 2019-07-26 | |||
| CN109576236A | 2019-04-05 | |||
| CN110592036A | 2019-12-20 | |||
| CN113969269A | 2022-01-25 | |||
| US20170253897A1 | 2017-09-07 |
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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