Title:
DECOY LIBRARY CONSTRUCTION METHOD AND APPARATUS, TARGET-DECOY LIBRARY CONSTRUCTION METHOD AND APPARATUS, AND METABOLOME FDR IDENTIFICATION METHOD AND APPARATUS
Document Type and Number:
WIPO Patent Application WO/2021/004355
Kind Code:
A1
Abstract:
Disclosed are a decoy library construction method and apparatus, a target-decoy library construction method and apparatus and a metabolome FDR identification method and apparatus. The decoy library construction method comprises: S1, respectively comparing a mass-to-charge ratio M of a parent ion of a metabolite of each spectrogram in a target database with those of all other spectrograms in the target database one by one, storing a spectrogram, a mass-to-charge ratio of a daughter ion of which is equal to M, and/or the serial number of the spectrogram in a signal spectrum index array, and generating a signal spectrum index two-dimensional array; S2, traversing all elements in the signal spectrum index two-dimensional array to obtain n arrays D, wherein the n arrays D constitute a decoy library signal array; and S3, copying parent ion information of the spectrogram, corresponding to each sub-set in the decoy library signal array, in the target database to the decoy library signal array to form a decoy library. By means of the metabolome identification method, FDR quality control can be carried out on an identification result, and a spectrogram can be quickly identified in high throughput, thereby improving the utilization rate of the spectrogram and the coverage of metabolite identification.
Inventors:
LI DEHUA (CN)
LI WEI (CN)
LUAN ENHUI (CN)
LONG QIAOYUN (CN)
SONG JIAPING (CN)
LI ZHENYU (CN)
WANG YALAN (CN)
LI WEI (CN)
LUAN ENHUI (CN)
LONG QIAOYUN (CN)
SONG JIAPING (CN)
LI ZHENYU (CN)
WANG YALAN (CN)
Application Number:
PCT/CN2020/099769
Publication Date:
January 14, 2021
Filing Date:
July 01, 2020
Export Citation:
Assignee:
SHENZHEN WEBIO CO LTD (CN)
SHENZHEN DIGITAL LIFE INST (CN)
SHENZHEN DIGITAL LIFE INST (CN)
International Classes:
G01N33/68
Domestic Patent References:
WO2018138901A1 | 2018-08-02 |
Foreign References:
CN101871945A | 2010-10-27 | |||
CN104034792A | 2014-09-10 | |||
CN102495127A | 2012-06-13 | |||
CN105334279A | 2016-02-17 |
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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