Title:
DEEP-CELL SUPER-RESOLUTION IMAGING METHOD AND SYSTEM AND PRISM OPTICAL SHEET APPARATUS
Document Type and Number:
WIPO Patent Application WO/2015/089910
Kind Code:
A1
Abstract:
A deep-cell super-resolution imaging method and system and a prism optical sheet apparatus are provided. The imaging method uses super-resolution optical fluctuation imaging (SOFI) and super-resolution localisation microscopy (LM), can acquire deep-cell super-resolution imaging by using a computer to eliminate background noise, and can be directly applied to an ordinary fluorescent microscope without requiring modification to the original optical structure. The imaging system loads the prism optical sheet apparatus onto an inverted microscope and uses a physical method to reduce the background noise, obtaining deep-cell super-resolution images by means of localisation microscopy, and can be directly loaded on to a traditional inverted fluorescent microscope.
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Inventors:
LOY MING TAK MICHAEL (CN)
DU SHENGWANG (CN)
ZHAO TENG (CN)
WANG YING (CN)
DU SHENGWANG (CN)
ZHAO TENG (CN)
WANG YING (CN)
Application Number:
PCT/CN2014/001138
Publication Date:
June 25, 2015
Filing Date:
December 17, 2014
Export Citation:
Assignee:
UNIV HONG KONG SCIENCE & TECHN (CN)
International Classes:
G01N21/64
Foreign References:
| CN200982860Y | 2007-11-28 | |||
| US20090242798A1 | 2009-10-01 | |||
| US20110249866A1 | 2011-10-13 | |||
| CN101655460A | 2010-02-24 | |||
| CN103308496A | 2013-09-18 | |||
| US20090242801A1 | 2009-10-01 |
Other References:
DERTINGER ET AL.: "Fast, background free, 3D super-resolution optical fluctuation imaging(SOFI)", PROC. NATL. ACAD. SCI., vol. 106, no. 52, 31 December 2009 (2009-12-31), pages 1 - 4
DEDECKER P ET AL.: "Localizer: fast, accurate, open-source, and modular software package for Superresolution microscopy", JOURNAL FO BIOMEDICAL OPTICS, vol. 12, no. 17, 3 December 2012 (2012-12-03), pages 126008 - 1 -126008-2
YING S HU ET AL.: "Light-sheet Bayesian microscopy enables deep- cell super-resolution imaging of heterochromatin in live human embryonic stem cells", OPTICAL NANOSCOPY, vol. 1, no. 2, 20 November 2013 (2013-11-20), pages 1 - 5
DEDECKER P ET AL.: "Localizer: fast, accurate, open-source, and modular software package for Superresolution microscopy", JOURNAL FO BIOMEDICAL OPTICS, vol. 12, no. 17, 3 December 2012 (2012-12-03), pages 126008 - 1 -126008-2
YING S HU ET AL.: "Light-sheet Bayesian microscopy enables deep- cell super-resolution imaging of heterochromatin in live human embryonic stem cells", OPTICAL NANOSCOPY, vol. 1, no. 2, 20 November 2013 (2013-11-20), pages 1 - 5
Attorney, Agent or Firm:
INSIGHT INTELLECTUAL PROPERTY LIMITED (CN)
北京英赛嘉华知识产权代理有限责任公司 (CN)
北京英赛嘉华知识产权代理有限责任公司 (CN)
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