Title:
DEGREASING COMPOSITION FOR CLEARING BIOLOGICAL TISSUE
Document Type and Number:
WIPO Patent Application WO/2023/010845
Kind Code:
A1
Abstract:
A degreasing composition for clearing biological tissue, a use in the preparation of a degreasing reagent, a degreasing reagent, a clearing kit comprising the degreasing composition or the degreasing reagent, a biological tissue clearing treatment method using the degreasing reagent, and an imaging method for clearing biological tissue comprising the steps of the biological tissue clearing treatment method. The degreasing composition comprises urea, N-butyldiethanolamine and Triton X-100, wherein the mass ratio of urea, N-butyldiethanolamine and Triton X-100 is (0.6-5):(0.3- 3): 1. The degreasing composition has a fast degreasing speed, good degreasing capabilities for large-volume biological samples, and may retain the structural features of biological tissues well from the cellular level to the subcellular level.
Inventors:
GAO LIANG (CN)
FENG RUILI (CN)
CHEN YANLU (CN)
FENG RUILI (CN)
CHEN YANLU (CN)
Application Number:
PCT/CN2022/079852
Publication Date:
February 09, 2023
Filing Date:
March 09, 2022
Export Citation:
Assignee:
UNIV WESTLAKE (CN)
International Classes:
G01N1/28; G01N21/64; G01N33/48
Domestic Patent References:
| WO2018224289A1 | 2018-12-13 |
Foreign References:
| CN113189071A | 2021-07-30 | |||
| KR20190114728A | 2019-10-10 | |||
| CN110068559A | 2019-07-30 | |||
| CN108445206A | 2018-08-24 | |||
| CN107132101A | 2017-09-05 |
Other References:
LU, MINGZHI: "Application of Urea in Transparent Specimen Making", CHINESE JOURNAL OF ANATOMY, vol. 41, no. 6, 1 January 2018 (2018-01-01), XP093033475
Attorney, Agent or Firm:
KINGSOUND & PARTNERS (CN)
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