Title:
DELIVERY METHOD OF TARGET MATERIAL INTO EXTRACELLULAR VESICLES USING EXTRACORPOREAL SHOCKWAVE
Document Type and Number:
WIPO Patent Application WO/2018/221954
Kind Code:
A1
Abstract:
The present disclosure relates to a method of delivering target materials into extracellular vesicles including exposing the target materials and the extracellular vesicles to extracorporeal shockwaves, a method of preparing target material-introduced extracellular vesicles, extracellular vesicles prepared by the method, drug delivery vehicles including extracellular vesicles, and a method of delivering target materials into cells. The present disclosure exposes extracellular vesicles derived from natural organisms such as animal cells, plant cells, and microorganisms including bacteria and eukaryotic bacteria as well as artificially produced extracellular vesicles to extracorporeal shockwaves extracellularly. Thus, the high-level energy extracorporeal shockwaves can be used to deliver the target material into the extracellular vesicle efficiently. When treating with extracorporeal shockwaves, the ability of target material-introduced extracellular vesicles to incorporate into target cells also increases. According to the method of the present disclosure, the target materials can be delivered into cells with high efficiency, being utilized in various fields.
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Inventors:
KWON KI HWAN (KR)
CHUNG JI HWA (KR)
KIM KYOUNG HWA (KR)
CHUNG JI HWA (KR)
KIM KYOUNG HWA (KR)
Application Number:
PCT/KR2018/006143
Publication Date:
December 06, 2018
Filing Date:
May 30, 2018
Export Citation:
Assignee:
UNIV EWHA IND COLLABORATION (KR)
International Classes:
C12N15/87; C12M1/12; C12N13/00
Foreign References:
| KR101719569B1 | 2017-03-27 | |||
| JP2007167006A | 2007-07-05 |
Other References:
HA, CHANG HOON ET AL.: "Novel mechanism of gene transfection by low-energy shock wave", SCIENTIFIC REPORTS, vol. 5, 2015, pages 12843, XP055562576
KODAMA, TETSUYA ET AL.: "Shock wave-mediated molecular delivery into cells", BIOCHIMICA ET BIOPHYSICA ACTA (BBA)-MOLECULAR CELL RESEARCH, vol. 1542, 2002, pages 186 - 194, XP004341334
TALLER, DANIEL ET AL.: "On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis", LAB ON A CHIP, vol. 15, no. 7, 2015, pages 1656 - 1666, XP055431779
KODAMA, TETSUYA ET AL.: "Shock wave-mediated molecular delivery into cells", BIOCHIMICA ET BIOPHYSICA ACTA (BBA)-MOLECULAR CELL RESEARCH, vol. 1542, 2002, pages 186 - 194, XP004341334
TALLER, DANIEL ET AL.: "On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis", LAB ON A CHIP, vol. 15, no. 7, 2015, pages 1656 - 1666, XP055431779
Attorney, Agent or Firm:
KIM, Soon Woong (KR)
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