Title:
DEOXYRIBONUCLEOTIDES MANUFACTURING BY ENZYMATIC REDUCTION OF RIBONUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO2004057010
Kind Code:
A3
Abstract:
A method for in vitro preparation of deoxyribonucleotides is disclosed. The deoxyribonucleotides in the present invention are converted from ribonucleotides extracted from yeast in the presence of E. coli RNA reductase and a reducing agent.
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Inventors:
YU KUO-MING
Application Number:
PCT/IB2003/006168
Publication Date:
October 14, 2004
Filing Date:
December 23, 2003
Export Citation:
Assignee:
SCINOPHARM SINGAPORE PTE LTD (SG)
International Classes:
C07H19/10; C07H19/20; C12P19/30; C12P19/32; (IPC1-7): C12P19/30; C12P19/32; C07H19/10; C07H19/20
Foreign References:
| EP0354610A1 | 1990-02-14 | |||
| US4303680A | 1981-12-01 | |||
| US3138539A | 1964-06-23 |
Other References:
JORDAN A ET AL: "Ribonucleotide reductases.", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 67, 1998, pages 71 - 98, XP009033963, ISSN: 0066-4154
BRUNELLA, A. ET AL: "Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase as biocatalyst in the preparative synthesis of 2'-deoxyribonucleoside-5'- triphosphates", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC , CODEN: JMCEF8; ISSN: 1381-1177, vol. 10, no. 1-3, 4 October 2000 (2000-10-04), pages 215 - 222, XP002289515
KUNINAKA A ET AL: "EXTRACTION OF RNA FROM YEAST PACKED INTO COLUMN WITHOUT ISOMERIZATION", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 44, no. 8, 1980, pages 1821 - 1827, XP002001948, ISSN: 0002-1369
BRUNELLA, ANDRE ET AL: "Preparative 2'-reduction of ATP catalyzed by ribonucleotide reductase purified by liquid-liquid extraction", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY , CODEN: BBBIEJ; ISSN: 0916-8451, vol. 64, no. 9, September 2000 (2000-09-01), pages 1836 - 1841, XP001194544
KOLLAROVA, MARTA ET AL: "Properties of ribonucleotide reductase from Streptomyces aureofaciens", BIOLOGIA, CODEN: BLOAAO; ISSN: 0006-3088, vol. 38, no. 12, 1983, BRATISLAVA, SLOVAKIA, pages 1189 - 1195, XP009034132
GLEASON, FLORENCE K. ET AL: "Adenosylcobalamin-dependent ribonucleotide reductase from the blue-green alga, Anabaena Sp. Purification and partial characterization", JOURNAL OF BIOLOGICAL CHEMISTRY , CODEN: JBCHA3; ISSN: 0021-9258, vol. 255, no. 16, 25 August 1980 (1980-08-25), pages 7728 - 7733, XP002289516
SINHABABU, ACHINTYA K. ET AL: "High-performance liquid chromatographic purification, optimization of the assay, and properties of ribonucleoside diphosphate reductase from rabbit bone marrow", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS , CODEN: ABBIA4; ISSN: 0003-9861, vol. 317, no. 1, 20 February 1995 (1995-02-20), pages 285 - 291, XP002289517
HOFER A ET AL: "Allosteric regulation of Trypanosoma brucei ribonucleotide reductase studied in vitro and in vivo.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 51, 18 December 1998 (1998-12-18), pages 34098 - 34104, XP002289518, ISSN: 0021-9258
WILLING A ET AL: "Nucleotide and thioredoxin specificity of the manganese ribonucleotide reductase from Brevibacterium ammoniagenes.", EUROPEAN JOURNAL OF BIOCHEMISTRY / FEBS, vol. 175, no. 1, 15 July 1988 (1988-07-15), pages 167 - 173, XP009034034, ISSN: 0014-2956
BRUNELLA, A. ET AL: "Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase as biocatalyst in the preparative synthesis of 2'-deoxyribonucleoside-5'- triphosphates", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC , CODEN: JMCEF8; ISSN: 1381-1177, vol. 10, no. 1-3, 4 October 2000 (2000-10-04), pages 215 - 222, XP002289515
KUNINAKA A ET AL: "EXTRACTION OF RNA FROM YEAST PACKED INTO COLUMN WITHOUT ISOMERIZATION", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 44, no. 8, 1980, pages 1821 - 1827, XP002001948, ISSN: 0002-1369
BRUNELLA, ANDRE ET AL: "Preparative 2'-reduction of ATP catalyzed by ribonucleotide reductase purified by liquid-liquid extraction", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY , CODEN: BBBIEJ; ISSN: 0916-8451, vol. 64, no. 9, September 2000 (2000-09-01), pages 1836 - 1841, XP001194544
KOLLAROVA, MARTA ET AL: "Properties of ribonucleotide reductase from Streptomyces aureofaciens", BIOLOGIA, CODEN: BLOAAO; ISSN: 0006-3088, vol. 38, no. 12, 1983, BRATISLAVA, SLOVAKIA, pages 1189 - 1195, XP009034132
GLEASON, FLORENCE K. ET AL: "Adenosylcobalamin-dependent ribonucleotide reductase from the blue-green alga, Anabaena Sp. Purification and partial characterization", JOURNAL OF BIOLOGICAL CHEMISTRY , CODEN: JBCHA3; ISSN: 0021-9258, vol. 255, no. 16, 25 August 1980 (1980-08-25), pages 7728 - 7733, XP002289516
SINHABABU, ACHINTYA K. ET AL: "High-performance liquid chromatographic purification, optimization of the assay, and properties of ribonucleoside diphosphate reductase from rabbit bone marrow", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS , CODEN: ABBIA4; ISSN: 0003-9861, vol. 317, no. 1, 20 February 1995 (1995-02-20), pages 285 - 291, XP002289517
HOFER A ET AL: "Allosteric regulation of Trypanosoma brucei ribonucleotide reductase studied in vitro and in vivo.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 51, 18 December 1998 (1998-12-18), pages 34098 - 34104, XP002289518, ISSN: 0021-9258
WILLING A ET AL: "Nucleotide and thioredoxin specificity of the manganese ribonucleotide reductase from Brevibacterium ammoniagenes.", EUROPEAN JOURNAL OF BIOCHEMISTRY / FEBS, vol. 175, no. 1, 15 July 1988 (1988-07-15), pages 167 - 173, XP009034034, ISSN: 0014-2956
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