Title:
DETECTION OF BIOLOGICAL THREAT AGENTS
Document Type and Number:
WIPO Patent Application WO2004031721
Kind Code:
A3
Abstract:
The technical features mentioned in the abstract do not include a reference sign between parentheses (PCT Rule 8.1(d)). Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated "L", 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagentanalyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof. Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated "L", 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagent-analyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof.
Inventors:
BARNES PHILLIP D (US)
BRILLSON LEONARD (US)
LEE STEPHEN C (US)
BRILLSON LEONARD (US)
LEE STEPHEN C (US)
Application Number:
PCT/US2003/029588
Publication Date:
July 01, 2004
Filing Date:
September 22, 2003
Export Citation:
Assignee:
UNIV OHIO STATE RES FOUND (US)
BARNES PHILLIP D (US)
BRILLSON LEONARD (US)
LEE STEPHEN C (US)
BARNES PHILLIP D (US)
BRILLSON LEONARD (US)
LEE STEPHEN C (US)
International Classes:
C12N1/00; C25B1/00; C25B9/04; G01N25/22; G01N27/00; G01N27/416; G01N33/53; G01N33/543; G01N; (IPC1-7): C25B9/04; C12N1/00; C25B1/00; G01N25/22; G01N27/00; G01N27/416; G01N33/53
Foreign References:
| US4490216A | 1984-12-25 | |||
| US5827482A | 1998-10-27 | |||
| US6326215B1 | 2001-12-04 | |||
| US6123819A | 2000-09-26 | |||
| US6175752B1 | 2001-01-16 | |||
| US5466348A | 1995-11-14 |
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