Title:
DETECTION OF BLOOD PLASMA SCHIZADRIN B OF DISSIPATING BLOOD STASIS BOTANICAL
Document Type and Number:
WIPO Patent Application WO/2008/131647
Kind Code:
A1
Abstract:
A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5mins, centrifugating at 9600rpm for 10mins, drying and enriching the upper layer at 25-30°C, and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C18, 2.1´100mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.
Inventors:
MA YUEMING (CN)
SHI RONG (CN)
ZHANG YONGYU (CN)
SHI RONG (CN)
ZHANG YONGYU (CN)
Application Number:
PCT/CN2008/000865
Publication Date:
November 06, 2008
Filing Date:
April 28, 2008
Export Citation:
Assignee:
SHANGHAI SUNDISE CHINESE MEDIC (CN)
MA YUEMING (CN)
SHI RONG (CN)
ZHANG YONGYU (CN)
MA YUEMING (CN)
SHI RONG (CN)
ZHANG YONGYU (CN)
International Classes:
G01N30/72; G01N30/36; G01N33/15; G01N33/49
Domestic Patent References:
| WO2001041778A1 | 2001-06-14 | |||
| WO2007020382A2 | 2007-02-22 |
Foreign References:
| CN101042380A | 2007-09-26 | |||
| CN1925864A | 2007-03-07 | |||
| JPH01165583A | 1989-06-29 | |||
| US6605305B2 | 2003-08-12 |
Other References:
DOU Z. ET AL.: "Analysis of lignans in serum of rats after oral administration of compound Wurenchun capsules by UPLC-MS/MS", CHINESE TRADITIONAL PATENT MEDICINE, vol. 29, no. 4, April 2007 (2007-04-01), pages 550 - 555, XP008126412
YAN J. ET AL.: "Pharmacokinetics study of schisandrin in Shengmai granule", TRADITIONAL CHINESE DRUG RESEARCH & CLINICAL PHARMACOLOGY, vol. 17, no. 1, January 2006 (2006-01-01), pages 36 - 39, XP008132024
WANG S. ET AL.: "Pharmacokinetic study of schizandrin in Wuling capsules in mice", LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH, vol. 15, no. 8, 2004, pages 468 - 470, XP008132023
YAN J. ET AL.: "Pharmacokinetics study of schisandrin in Shengmai granule", TRADITIONAL CHINESE DRUG RESEARCH & CLINICAL PHARMACOLOGY, vol. 17, no. 1, January 2006 (2006-01-01), pages 36 - 39, XP008132024
WANG S. ET AL.: "Pharmacokinetic study of schizandrin in Wuling capsules in mice", LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH, vol. 15, no. 8, 2004, pages 468 - 470, XP008132023
Attorney, Agent or Firm:
SHANGHAI SHEN HUI PATENT AGENT LTD. (103 Cao Bao Road, Shanghai 3, CN)
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