Title:
DETECTION OF DINOFLAGELLATES
Document Type and Number:
WIPO Patent Application WO/2014/016706
Kind Code:
A3
Abstract:
Provided herein is technology relating to detecting saxitoxin-producing dinoflagellates and particularly, but not exclusively, to methods and compositions for detecting dinoflagellates by amplification of the sxtG gene.
Inventors:
ORR RUSSELL (NO)
STÜKEN ANKE (NO)
JAKOBSEN KJETILL SIGURD (NO)
STÜKEN ANKE (NO)
JAKOBSEN KJETILL SIGURD (NO)
Application Number:
PCT/IB2013/002481
Publication Date:
April 24, 2014
Filing Date:
May 14, 2013
Export Citation:
Assignee:
ORR RUSSELL (NO)
STÜKEN ANKE (NO)
JAKOBSEN KJETILL SIGURD (NO)
UNIV OSLO (NO)
STÜKEN ANKE (NO)
JAKOBSEN KJETILL SIGURD (NO)
UNIV OSLO (NO)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2009129558A1 | 2009-10-29 |
Other References:
ANKE STÜKEN ET AL: "Discovery of Nuclear-Encoded Genes for the Neurotoxin Saxitoxin in Dinoflagellates", PLOS ONE, vol. 6, no. 5, 18 May 2011 (2011-05-18), pages e20096 - e20096, XP055103523, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0020096
DATABASE EMBL [online] 12 September 2009 (2009-09-12), "Mus musculus NOD/ShiLtJ GSS, clone CH29-191C12 3' sequence", XP002720733, retrieved from EBI accession no. EM_GSS:FR342681 Database accession no. FR342681
DATABASE Geneseq [online] 3 June 2004 (2004-06-03), "NEPHA gene transcriptional control region YY1 binding site.", XP002720734, retrieved from EBI accession no. GSN:ADM76355 Database accession no. ADM76355
DATABASE Geneseq [online] 16 December 1998 (1998-12-16), "ERAB protein coding sequence.", XP002720735, retrieved from EBI accession no. GSN:AAV60576 Database accession no. AAV60576
DATABASE EMBL [online] 12 July 2007 (2007-07-12), "G772P514FF18.T0 Culex pipiens quinquefasciatus Bacteria Infected Library Culex pipiens quinquefasciatus cDNA, mRNA sequence.", XP002720736, retrieved from EBI accession no. EM_EST:EV315883 Database accession no. EV315883
S. A. MURRAY ET AL: "sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 19, October 2011 (2011-10-01), pages 7050 - 7057, XP055103261, ISSN: 0099-2240, DOI: 10.1128/AEM.05308-11
RALF KELLMANN ET AL: "Biosynthesis and Molecular Genetics of Polyketides in Marine Dinoflagellates", MARINE DRUGS, vol. 8, no. 4, 31 March 2010 (2010-03-31), pages 1011 - 1048, XP055103260, ISSN: 1660-3397, DOI: 10.3390/md8041011
AMANDA J FOSS ET AL: "Characterization of paralytic shellfish toxins from Lyngbya wollei dominated mats collected from two Florida springs", HARMFUL ALGAE, ELSEVIER, AMSTERDAM, NL, vol. 16, 8 February 2012 (2012-02-08), pages 98 - 107, XP028472965, ISSN: 1568-9883, [retrieved on 20120217], DOI: 10.1016/J.HAL.2012.02.004
R. J. S. ORR ET AL: "Evolutionary Acquisition and Loss of Saxitoxin Biosynthesis in Dinoflagellates: the Second "Core" Gene, sxtG", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 7, 18 January 2013 (2013-01-18), pages 2128 - 2136, XP055103259, ISSN: 0099-2240, DOI: 10.1128/AEM.03279-12
DATABASE EMBL [online] 12 September 2009 (2009-09-12), "Mus musculus NOD/ShiLtJ GSS, clone CH29-191C12 3' sequence", XP002720733, retrieved from EBI accession no. EM_GSS:FR342681 Database accession no. FR342681
DATABASE Geneseq [online] 3 June 2004 (2004-06-03), "NEPHA gene transcriptional control region YY1 binding site.", XP002720734, retrieved from EBI accession no. GSN:ADM76355 Database accession no. ADM76355
DATABASE Geneseq [online] 16 December 1998 (1998-12-16), "ERAB protein coding sequence.", XP002720735, retrieved from EBI accession no. GSN:AAV60576 Database accession no. AAV60576
DATABASE EMBL [online] 12 July 2007 (2007-07-12), "G772P514FF18.T0 Culex pipiens quinquefasciatus Bacteria Infected Library Culex pipiens quinquefasciatus cDNA, mRNA sequence.", XP002720736, retrieved from EBI accession no. EM_EST:EV315883 Database accession no. EV315883
S. A. MURRAY ET AL: "sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 19, October 2011 (2011-10-01), pages 7050 - 7057, XP055103261, ISSN: 0099-2240, DOI: 10.1128/AEM.05308-11
RALF KELLMANN ET AL: "Biosynthesis and Molecular Genetics of Polyketides in Marine Dinoflagellates", MARINE DRUGS, vol. 8, no. 4, 31 March 2010 (2010-03-31), pages 1011 - 1048, XP055103260, ISSN: 1660-3397, DOI: 10.3390/md8041011
AMANDA J FOSS ET AL: "Characterization of paralytic shellfish toxins from Lyngbya wollei dominated mats collected from two Florida springs", HARMFUL ALGAE, ELSEVIER, AMSTERDAM, NL, vol. 16, 8 February 2012 (2012-02-08), pages 98 - 107, XP028472965, ISSN: 1568-9883, [retrieved on 20120217], DOI: 10.1016/J.HAL.2012.02.004
R. J. S. ORR ET AL: "Evolutionary Acquisition and Loss of Saxitoxin Biosynthesis in Dinoflagellates: the Second "Core" Gene, sxtG", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 7, 18 January 2013 (2013-01-18), pages 2128 - 2136, XP055103259, ISSN: 0099-2240, DOI: 10.1128/AEM.03279-12
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