Title:
DETECTION KIT FOR INTERLEUKIN-6
Document Type and Number:
WIPO Patent Application WO/2015/165357
Kind Code:
A1
Abstract:
A detection kit for interleukin-6 comprises two specific monoclonal antibodies of the interleukin-6. A monoclonal antibody covering a perforated plate, a monoclonal antibody labeled by biotin, and interleukin-6 of a sample to be detected form a sandwich compound structure of "covering antibody-antigen-biotin labeled antibody". The biotin labeled on the antibody is combined with streptavidin labeled by HRP, and then the HRP reacts with a substrate to generate a signal that is used for conducting quantitative analysis on the interleukin-6. The kit can quantitatively detect the content of the interleukin-6 in the sample rapidly and sensitively.
Inventors:
YU HUI (CN)
LI YUXIN (CN)
WANG XU (CN)
CHEN QINHUI (CN)
LI XIN (CN)
LIU QI (CN)
LI YUXIN (CN)
WANG XU (CN)
CHEN QINHUI (CN)
LI XIN (CN)
LIU QI (CN)
Application Number:
PCT/CN2015/077288
Publication Date:
November 05, 2015
Filing Date:
April 23, 2015
Export Citation:
Assignee:
BEIJING PERGRANDE BIOTECH DEV CO LTD (CN)
International Classes:
G01N33/577; C07K16/24; C12N5/20; G01N33/543
Foreign References:
| CN103969438A | 2014-08-06 | |||
| CN101393204A | 2009-03-25 | |||
| JP2884628B2 | 1999-04-19 | |||
| CN101201357B | 2012-06-06 | |||
| US8062864B2 | 2011-11-22 | |||
| CN101221178A | 2008-07-16 | |||
| CN102740888A | 2012-10-17 | |||
| CN103048465A | 2013-04-17 |
Other References:
ZHANG, HUILI ET AL.: "Identification and application of monoclonal antibodies against IL -6", MDECULAR CARDIOLOGY OF CHINA, vol. 3, no. 3, 30 June 2003 (2003-06-30), pages 152 - 156
CHEN, LIN ET AL.: "The Preparation and Preliminary Determination of Anti- IL -6 Monoclonal Antibody", IMMUNOLOGICAL JOURNAL, vol. 15, no. 1, 31 January 1999 (1999-01-31), pages 54 - 56
MAARTEN, H. ET AL.: "Sensitive ELISA for interleukin-6: Detection of IL -6 in biological fluids: synovial fluids and sera", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 138, no. 1, 18 April 1991 (1991-04-18), pages 47 - 56, XP023986985, ISSN: 0022-1759
CHEN, LIN ET AL.: "The Preparation and Preliminary Determination of Anti- IL -6 Monoclonal Antibody", IMMUNOLOGICAL JOURNAL, vol. 15, no. 1, 31 January 1999 (1999-01-31), pages 54 - 56
MAARTEN, H. ET AL.: "Sensitive ELISA for interleukin-6: Detection of IL -6 in biological fluids: synovial fluids and sera", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 138, no. 1, 18 April 1991 (1991-04-18), pages 47 - 56, XP023986985, ISSN: 0022-1759
Attorney, Agent or Firm:
GE CHENG & CO., LTD. (CN)
北京戈程知识产权代理有限公司 (CN)
北京戈程知识产权代理有限公司 (CN)
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