Title:
DEVICE FOR AUTOMATING NUCLEIC ACID AMPLIFICATION, AND DEVICE FOR AUTOMATING NUCLEIC ACID AMPLIFICATION ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2015/141649
Kind Code:
A1
Abstract:
This device for automating nucleic acid amplification executes a emulsion generation step for including beads to which a nucleic acid is bonded in a water-in-oil type emulsion, a nucleic acid amplification step for amplifying the nucleic acid in the emulsion, an emulsion breaking step for breaking the emulsion, and a purification and concentration step for purifying and concentrating the amplified nucleic acid. The device for automatic nucleic acid amplification has a dispenser unit (20) provided with a dispenser (21) for dispensing solutions and/or reagents to each step, an emulsion unit (30) for executing the emulsion generation step and the emulsion breaking step, a nucleic acid amplification unit (40) for executing the nucleic acid amplification step using the emulsion generated by the emulsion unit (40), and a purification and concentration unit (50) for executing the purification and concentration step using a solution including the amplified nucleic acid obtained by the emulsion breaking step in the emulsion unit (40).
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Inventors:
TAJIMA HIDEJI (JP)
Application Number:
PCT/JP2015/057782
Publication Date:
September 24, 2015
Filing Date:
March 17, 2015
Export Citation:
Assignee:
UNIVERSAL BIO RESEARCH CO LTD (JP)
International Classes:
C12M1/00; C12Q1/68
Domestic Patent References:
| WO2010117457A2 | 2010-10-14 | |||
| WO2006051901A1 | 2006-05-18 | |||
| WO2005073410A2 | 2005-08-11 |
Foreign References:
| JP2013535193A | 2013-09-12 | |||
| JPH0662897A | 1994-03-08 | |||
| JP2009540829A | 2009-11-26 | |||
| JP2013507108A | 2013-03-04 | |||
| JP2007523627A | 2007-08-23 | |||
| US20130164789A1 | 2013-06-27 |
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
Kobayashi 浩 (JP)
Kobayashi 浩 (JP)
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