Title:
DEVICE FOR ISOLATING CELL FRACTIONS FROM HUMAN AND ANIMAL TISSUES AND METHOD FOR THE USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/078563
Kind Code:
A8
Abstract:
The present invention relates to medical biotechnology and cell technology. Proposed is a method for isolating stromal vascular fraction of adipose tissue, which includes: decantation of a lipoaspirate; delicate washing of the lipoaspirate in buffer solutions; enzymatic treatment using a mixture of collagenases, thermolysin, dispase and trypsin at a temperature of 37ºC; centrifugation at 500-2000 g and filtration through microfilters with a pore size of 10-300 microns with the subsequent removal of fat and stromal tissue matter; repeat washing, and concentration of the cell fraction, wherein all of the stages of the process are carried out under closed system conditions. A corresponding device is configured as a sealed container consisting of two chambers, which are separated from one another by nylon microfilters with pore sizes of 10-500 microns. The device comprises fittings, provided with valves, and channels for the introduction and removal of biological tissue and a cellular end product for subsequent administration. Owing to the presence of a cocktail of mature and progenitor cells in stromal vascular fraction, which have a paracrine effect, the sterility of all of the stages of the process and the safety thereof, the claimed method can be used in medicine for the cell-based therapy and regeneration of organs and tissues, including: soft tissue and bone regeneration, and the treatment of cosmetic defects, chronic trophic and radiation ulcers, burns, Crohn's disease, multiple sclerosis, graft-versus-host reactions, myocardial infarction and strokes of different origins.
Inventors:
VEREMEYEV ALEKSEY VLADIMIROVICH (RU)
KATS NATAN GEORGIYEVICH (US)
NESTERENKO VLADIMIR GEORGIYEVICH (RU)
BOLGARIN ROMAN NIKOLAYEVICH (RU)
PETKOVA MARIYA ALEKSANDROVNA (RU)
KATS NATAN GEORGIYEVICH (US)
NESTERENKO VLADIMIR GEORGIYEVICH (RU)
BOLGARIN ROMAN NIKOLAYEVICH (RU)
PETKOVA MARIYA ALEKSANDROVNA (RU)
Application Number:
PCT/RU2015/000738
Publication Date:
May 17, 2018
Filing Date:
November 03, 2015
Export Citation:
Assignee:
JOINTECHCELL LLC JTC LLC (RU)
International Classes:
C12M3/06; A61K35/12; A61P41/00; C12M1/12; C12N5/077
Attorney, Agent or Firm:
PUSTOVALOVA, Maria Leonidovna et al. (RU)
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