Title: Device and method for cleansing the skin and/or keratin fibers

Technical field

The present invention relates to cleansing the skin and/or keratin fibers, such as the head hair or the beard, in particular human keratin fibers but also animal keratin fibers, in particular body hairs, and/or mucus membranes and/or nails. In particular, the invention relates to a cleansing device containing a cleansing composition and to a method for cleaning the skin and/or keratin fibers, such as the head hair or the beard, and/or mucus membranes and/or nails, using such a device.

Prior art

The difficulty of being able to access running water or of having available a large volume of water is currently a reality for many people in the world and this difficulty might be accentuated in the coming decades from the viewpoint of climate change.

In this context, it is necessary to have available items of equipment which make it possible to consume less water, indeed even very little water, for common tasks for which we generally use a small amount or a great deal of water. This is a case with washing the body, for which we take baths or showers, consuming a large amount of water.

It thus appears necessary to make it possible to greatly reduce the use of water for cleansing the skin and/or keratin fibers.

Furthermore, the heating of a large amount of water is a source of CO2 emission if the energy source is not renewable; the decrease in CO2 emission is thus desirable.

US 2018/084888 relates to liquid applicators, particularly those used in hair care, and discloses a manifold dispensing cap having a base adapted to be attached to a bottle. The base seals the bottle and can move fluid from the interior of the bottle to a manifold. The manifold has a plurality of fingers which are evenly spaced radially from the centre where they extend outwardly. A central opening of the base acts as a supply port, and each finger is hollow having at its distal end an opening for the distribution of the fluid.

US 10 285 494 discloses a fluid dispenser including a shell with a handle portion and an applicator portion opposite to the handle portion. The fluid dispenser includes a handle connected to the handle portion of the shell, and an applicator connected to the applicator portion of the shell. The applicator includes a base portion and a plurality of nodules extending from the base portion opposite to the handle portion of the shell. An interior cavity is formed between the applicator and the shell containing the fluid to be dispensed flowing from an orifice through at least one distribution orifice.

JP 3 207092 describes a brush for cleaning the skin and the scalp, comprising a reservoir, a handle, a plurality of guide portions in relief and a plurality of head portions, each being connected to a guide portion. The reservoir comprises an upper part and a lower part which can be made in one piece or in two separate pieces that can be assembled. The lower part is made of a rigid material and carries the guide portions, the latter being made in one piece with the lower part or being assembled with the latter. The guide portions are preferably made of a rigid material.

US 1 113 843 describes a bottle secured to a hollow comb comprising teeth defining a passage terminated by an orifice. A valve secured to the comb closes the passage between the bottle and the comb.

The main objective of the invention is to provide a novel form of body cleansing, for the skin and/or keratin fibers, capable of effectively replacing conventional aqueous body cleansing, such as, for example, the shower, in particular hot shower, or the bath, in particular hot bath.

As described in detail below, the solution provided consists in making available a novel cleansing device containing a cleansing composition, the tool being permanent and suitable for precisely being freed from the use of a large amount of water for body cleansing, and also making available a body cleansing method which is based in particular on the use of this device.

Disclosure of the invention

A subject matter of the invention is thus, according to one of its aspects, a device for the packaging and dispensing of a composition for cleansing the skin and/or keratin fibers and/or mucus membranes and/or nails, comprising:

- a composition, in particular a cosmetic and nontherapeutic composition, for the care, hygiene and/or cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails, in the form of an aqueous solution comprising at least: (i) all or part of a lysate, isolated or not from a medium for the fermentation of a nonpathogenic and aerobic proteobacterium or a (glyco)oligopeptide fraction isolated from such a lysate, (ii) at least one mucopolysaccharide, in particular with a weight of greater than 10 kD,

(iii) at least one moisturizing agent distinct from (i) and (ii) and in particular chosen from glycerol, aminoglycerol, urea, their salts and their mixtures,

(iv) at least one electrolyte,

(v) water and,

(vi) if appropriate, at least one cosmetic active principle distinct from (i), (ii) and (iii),

- a body forming a reservoir for containing the composition, the body comprising at least one feed orifice for making possible the departure of the composition outside said body,

- at least one pad intended to be in contact with the skin and/or the keratin fibers and/or the mucus membranes and/or the nails, attached to the body and exhibiting an exterior surface on which are provided cleansing protrusions and at least one dispensing opening, the dispensing opening and said at least one feed orifice being arranged so as to make possible the dispensing of the composition. This novel device containing the composition makes it possible to carry out cleansing, including, for example, makeup removal or scrubbing, which is rapid, economical and independent of a shower, over all or part of the body or of the keratin fibers, in particular of the head hair, eyelashes, eyebrows or beard, and/or of the mucus membranes and/or of the nails.

The term “keratin fibers” is understood to mean, according to the invention, the eyelashes, eyebrows, body hair, beard, scalp and/or head hair.

The term “skin” is understood to mean all the skin of the body, and preferably the skin of the face, neckline, neck, arms and forearms, indeed even more preferably still the skin of the face, in particular of the forehead, nose, cheeks, chin and area around the eyes.

The term “cleansing” of the skin and/or keratin fibers and/or mucus membranes and/or nails is understood to mean any form of cleansing, including washing, shampooing, scrubbing, removing makeup, massaging, brushing, combing or any other action or body movement carried out on the skin and/or keratin fibers and/or mucus membranes and/or nails with the device using the cleansing protrusions and the dispensed composition. By virtue of the invention, the benefit is enjoyed of a novel device for cleansing the skin and/or keratin fibers, such as human head hair or the human beard or also the hairs of animals, and/or mucus membranes and/or nails, which is permanent and suitable for precisely being freed from the use of a large amount of water for cleansing the skin and/or keratin fibers.

This is because such a device makes it possible to carry out cleansing while requiring little water, in particular only the water present in the composition, which makes it possible to be freed from the presence of a running water point or of a large amount of water for carrying out cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails of a person. Such a device can thus be of use for washing, shampooing, removing makeup or scrubbing for a person of little or no mobility, and/or on each occasion when water is lacking or when it is desired to economize on it. This is because the cleansing protrusions make it possible to carry out, with the composition dispensed from the reservoir, autonomous and efficient cleansing, employing a rubbing and massaging body movement using the cleansing protrusions and without requiring a contribution of water apart from the cleansing composition, in the manner of the washing carried out by felines, such as cats, and other living creatures using their tongues and their saliva.

The cleansing protrusions can be configured in order to make it possible to carry out massaging, rubbing or brushing of the skin and/or keratin fibers and/or mucus membranes and/or nails with the pad.

All or part of the cleansing protrusions can comprise spikes.

In this case, at least a part of the spikes can be configured in order to imitate papillae of the tongue of a feline, in particular of a cat.

The term “spikes configured in order to imitate papillae of the tongue of a feline” is understood to mean spikes which imitate the shape of a papilla of the tongue of a feline, for example being curved in a similar way to that of a papilla. Of course, some parameters of this shape can be adapted in order to be better suited in particular to human head hair or animal fur. In particular, the spikes of the pad can be harder and/or longer than the papillae. This can make it possible to ensure efficient brushing of human head hair, for example, by going as far as the scalp, which can thus also be massaged. The cleansing protrusions can comprise at least one strip, in particular a plurality of strips, extending in particular substantially orthogonally from the exterior surface of the pad.

The pad is preferably removably attached to the body.

As regards the composition, it has the novel feature of being bioinspired insofar as its composition is prepared from the viewpoint of that of saliva.

For the record, saliva is a highly dilute fluid, more than 99% composed of water. The normal pH of saliva is from 6 to 7. It is composed of a variety of electrolytes, including sodium, potassium, calcium, magnesium, bicarbonate and phosphate ions. Immunoglobulins, proteins, enzymes, mucins and nitrogenous entities, such as urea and ammonia, are also present in saliva. The biocarbonates, phosphates and urea act to modulate the pH and the buffering power of the saliva. The macromolecular proteins and the mucins serve to cleanse, to aggregate and/or to attach oral microorganisms and contribute to the metabolism of dental plaque. The calcium ions, phosphate ions and proteins modulate in particular demineralization and remineralization. With regard to the immunoglobulins, proteins and enzymes, they contribute to the antibacterial action. Finally, as seromucosal coating, the saliva lubricates and protects the buccal tissues.

Contrary to all expectations, the inventors have found that a specific composition, prepared on the model of saliva, makes it possible to provide efficient cleansing of keratin materials, provided that it is used with a specific cleansing tool.

In addition, from the viewpoint of its components, the washing composition according to the invention provides benefits of lubrication or film forming for the skin and/or keratin fibers, in particular the head hair or the beard, and/or mucus membranes and/or nails and generates a novel sensory perception for the users, such as a pleasant sensory experience of softness and of silkiness.

According to an alternative embodiment, the composition contains all or a fraction of lysate isolated from its fermentation medium.

According to another alternative embodiment, the composition contains said whole lysate in a complete fermentation medium.

In particular, the bacterium belongs to the Vitreoscilla sp genus. More particularly, the bacterium of the composition is a Vitreoscilla filiformis strain. It is preferably the bacterium Vitreoscilla filiformis and more preferably the Vitreoscilla filiformis ATCC 15551 strain.

The composition can contain at least one mucopolysaccharide extracted from marine algae, in particular chosen from carrageenans, alginates, ulvans, fucoidans, chitosan, chitin, their salts, their extracts and their mixtures, and in particular containing at least one carrageenan in particular derived from Chondrus crispus or Kappaphycus alvarezii.

The composition can contain a single electrolyte or a mixture of several electrolytes chosen from alkaline earth metal salts and in particular calcium salts, alkali metal salts and in particular sodium and potassium salts, and also magnesium or manganese salts and carbonates, bicarbonates, sulfates, phosphates, bromides, chlorides and fluorides.

The composition of the device according to the invention comprises, for example, at least 50% by weight and in particular at least 80% by weight of an aqueous medium, with respect to its total weight.

The composition, which also comprises at least one electrolyte, is provided in the form of an aqueous solution.

Thus, the composition of the device according to the invention containing water, in the form of an aqueous solution, can be used as product for cleansing the body, such as a shower product, a product for the bath, a product for cleansing the hands, as a product for cleansing keratin fibers, in particular head hair or the beard, such as a shampoo, or also as product for cleansing the face, for example as makeup removing product for the eyes, the face or the lips, or else as product for cleansing or caring for the mucus membranes and/or the nails.

In a specific embodiment, the composition contains at least:

- at least the oligopeptide fraction isolated from a lysate from a medium for the fermentation of a proteobacterium Vitreoscilla filiformis, in particular the Vitreoscilla filiformis ATCC 15551 strain,

- approximately 0.05% by weight of at least one mucopolysaccharide, in particular at least one carrageenan, with respect to its total weight,

- approximately 15% by weight of at least one moisturizing agent chosen from glycerol, aminoglycerol, urea, their salts and their mixtures, with respect to its total weight,

- approximately 10% by weight of desalified seawater, with respect to its total weight, and - approximately 74% by weight of water, distinct from the seawater, with respect to its total weight.

Another subject matter of the present invention, according to another of its aspects, in combination with that which precedes, is a method for cleansing the skin and/or keratin fibers and/or mucus membranes and/or nails using a device as defined above, the method comprising the following stages:

(a) stage a: bringing about the dispensing of the composition by said at least one dispensing opening,

(b) stage b: bringing the dispensed composition and the cleansing protrusions into contact with the skin and/or keratin fibers and/or mucus membranes and/or nails,

(c) stage c: rubbing, massaging, brushing and/or combing the skin and/or keratin fibers and/or mucus membranes and/or nails using the cleansing protrusions.

When one or more strips are present on the pad, it is also possible to carry out scraping of the skin.

The present invention is also targeted, according to another of its aspects, in combination with that which precedes, at a nontherapeutic cosmetic method for scrubbing the skin comprising the application of at least one cosmetic composition using the device according to the invention.

The present invention is also targeted, according to another of its aspects, in combination with that which precedes, at a nontherapeutic cosmetic method for removing makeup from the eyelashes, lips and/or skin, in particular of the face, comprising the application of at least one cosmetic composition to said zone from which makeup is to be removed using the device according to the invention.

Device

The device advantageously comprises at least one valve making it possible or not to seal said at least one feed orifice.

The reservoir can thus be closed by sealing the feed orifice, which makes it possible to prevent the composition from flowing out of the reservoir in an uncontrolled way, which also makes it possible to prevent any escape of composition. When desired, in particular during the use of the device, it is possible to open the valve, making it possible to free the feed office, which is no longer sealed, in order to make it possible for the composition to pass out of the reservoir. This opening can be automatic and result from placing the cleansing composition under pressure.

The body can comprise at least one wall delimiting a volume forming the reservoir, at least a part of said wall being elastically deformable.

In this case, said at least one valve can be configured so as to make possible the retention of the composition in the reservoir in the absence of pressure exerted on said at least one elastically deformable part of the wall and the departure of composition out of the reservoir when a pressure is exerted on said at least one elastically deformable part of the wall. The valve can thus be closed at rest and opened under the effect of the pressure exerted by the user when the latter presses on the body in order to reduce its interior volume.

Said at least one valve exhibits, for example, a base of annular shape and a seal attached to the base and comprising at least two elastically deformable membranes interacting in the absence of pressure so as to prevent any departure of the composition out of the reservoir and defining, between them, an opening when a pressure is exerted on the elastically deformable part of the wall of the body in order to make possible the departure of the composition out of the reservoir.

The device preferably comprises at least one retaining element arranged in order to cause the valve to interact with the feed orifice, the retaining element forming, for example, a collar with an exterior diameter substantially equal to that of said feed orifice and with an interior diameter substantially equal to the exterior diameter of a part of the valve, so as to support the valve on the outside and to interact on the inside with the feed orifice.

In the case where the pad is removably attached to the body, at least two different pads can be provided, being capable of being attached alternatively to the body. The device can thus, for example, comprise a pad provided for the cleansing of the skin and a pad provided for the cleansing of the keratin fibers. In the latter case, the cleansing protrusions of the pad provided for the cleansing of the skin can be at least in part different from the cleansing protrusions of the pad provided for the cleansing of the keratin fibers.

The body can be made of at least one elastically deformable material, in particular of one elastically deformable material, for example chosen from the group consisting of a thermoplastic elastomer (TPE), a high-performance elastomer (HPE), in particular a high-performance polyurethane elastomer or a high-performance thermoplastic elastomer, a polyurethane, a silicone and a polyamide, a biocellulose and a fungus mycelium. The material chosen is preferably chosen from biopolymers or bioplastics, that is to say comprising biodegradable polymers, polymers resulting from renewable sources, for example biobased (obtained by a process starting from plant matter), or polymers belonging to both these categories. The material chosen is preferably a recyclable or reusable polymer. The material can be chosen, for example, from the range of biopolymers sold under the name Terraprene®, thermoplastic elastomer (TPE) compounds, by FKUR Polymers, or also from the range of thermoplastic elastomers (TPEs) containing starting materials resulting from renewable plant products sold under the name Dryflex® Green by Hexpol, or also from the range of biobased thermoplastic elastomers (BioTPEs) sold by NaturePlast.

The or each pad can be made of at least one elastically deformable material, in particular of one elastically deformable material, for example chosen from the group consisting of a thermoplastic elastomer (TPE), a high-performance elastomer (HPE), in particular a high-performance polyurethane elastomer or a high-performance thermoplastic elastomer, a polyurethane, a silicone and a polyamide, a biocellulose and a fungus mycelium. The material chosen is preferably chosen from biopolymers or bioplastics, that is to say comprising biodegradable polymers, polymers resulting from renewable sources, for example biobased (obtained by a process starting from plant matter), or polymers belonging to both these categories. The material chosen is preferably a recyclable or reusable polymer. The material can be chosen, for example, from the range of biopolymers sold under the name Terraprene®, thermoplastic elastomer (TPE) compounds, by FKUR Polymers, or also from the range of thermoplastic elastomers (TPEs) containing starting materials resulting from renewable plant products sold under the name Dryflex® Green by Hexpol, or also from the range of biobased thermoplastic elastomers (BioTPEs) sold by NaturePlast.

The materials constituting the body and the pad can be identical or different, in particular as regards their elasticity.

The pad provided for the cleansing of the skin or at least the cleansing protrusions of this pad can be more flexible than the pad provided for the cleansing of the keratin fibers or at least than the cleansing protrusions of this pad. When the pad is removably attached to the body, the pad advantageously comprises a fixing protrusion on an interior surface opposite the exterior surface, the body exhibiting, on an exterior face, a complementary fixing protrusion capable of interacting, in particular mechanically, with the fixing protrusion of the pad for the attachment of the pad to the body.

In this case, the complementary fixing protrusion of the body can delimit, at least partially, a zone comprising said feed orifice(s).

Still in this case, the complementary fixing protrusion of the body comprises, for example, a flange extending toward the exterior of the body with an at least partially closed outline, the outline of the flange exhibiting a shape similar to that of the exterior outline of the fixing protrusion of the pad, which fixing protrusion can form an edge configured to match the shape of the flange.

By virtue of the presence of fixing protrusions on the pad and on the body, which are capable of interacting with one another, it is easy to render the pad and the body integral and then nonintegral, in particular when the pad and/or the body is/are made of at least one material elastically deformable at least at the fixing protrusion.

In order to facilitate the removal of the pad, the latter can comprise at least one tongue, in particular two tongues, making it possible to grasp the pad in order to remove it from the body, when desired. This or these tongues can be present at at least one longitudinal end of the pad, in particular extending from an interior surface of the pad.

The pad having a longitudinal axis, the body having a longitudinal axis, the longitudinal axis of the pad and the longitudinal axis of the body may make an angle less than 45°, preferably being substantially parallel.

The body preferably comprises a plurality of feed orifices, in particular five feed orifices, in order to make possible the departure of the composition, said feed orifices being, for example, positioned in a row, in particular provided in order to spread out substantially at the middle of the pad when the latter is attached to the body. The feed orifices preferably are more than two, more preferably more than three, even more preferably more than four, and preferably less than ten, more preferably less than eight. The feed orifice(s) are through orifices. Having a plurality of feed orifices may allow to reduce the size of each orifice and provide the user better control over the flow of the dispensed composition as well as a better surface distribution of the composition. The device may have a plurality of valves, each valve configured for closing a respective feed orifice.

Each valve may have an axis that is oriented substantially perpendicularly to a longitudinal axis of the body. By axis of the valve, one should understand the direction along which the composition flows when the valve is open, the opening of the valve being caused by the pressure of the composition upwards the valve.

The valves may remain attached to the body in the absence of the pad.

In the case where the feed orifices are positioned in a row and in the case where the pad extends along a curved longitudinal axis, said row can be substantially curved with a curvature similar to a curvature of said curved longitudinal axis of the pad.

The dispensing opening can be formed on the exterior surface of the pad or in one of the cleansing protrusions.

The device may comprise for each feed orifice a respective dispensing opening.

In the case where the cleansing protrusions comprise spikes, at least one spike can exhibit a passage emerging on the dispensing opening.

In this case, the passage of said at least one spike which emerges on the dispensing opening can pass through the spike from an end connected to the exterior surface of the pad up to a free end, the free end comprising the dispensing opening for the dispensing of the cleansing composition.

All or part of the spikes can exhibit a shape with a diameter which decreases from the end connected to the exterior surface of the pad up to the free end.

Said at least one pad comprises, for example, spikes of at least one first shape and spikes of at least one second shape, differing in particular from the first shape in the diameter, the shape of the free end, the length and/or the straight or curved longitudinal axis along which the spikes extend between their end connected to the pad and their free end.

At least a part of the spikes, indeed even all of the spikes, of the pad, in particular the pad provided for the cleansing of the keratin fibers, can exhibit a curved shape, the longitudinal axis of said spike(s) then being curved.

At least a part of the spikes, in particular all of the spikes, of the pad, in particular the pad provided for the cleansing of the skin, can exhibit a rectilinear shape, in particular of substantially conical or frustoconical shape. When the cleansing protrusions comprise at least one strip, at least one region of the exterior surface of the pad, in particular of the pad provided for the cleansing of the skin, can be devoid of spikes, said region being, for example, provided with said at least one strip, in particular with a plurality of strips, extending substantially orthogonally from said exterior surface. In this case, the cleaning protrusions comprise, in a region of the exterior surface of the pad, spikes and, in at least one other region of the exterior surface of the pad, strips.

Such strips can have a scraping effect on the skin, for example for removing the remaining composition and/or dead skin cells, makeup to be removed or impurities. The strips can be arranged parallel to one another and/or parallel to the exterior edge or outline of the exterior surface of the pad in said region. It is understood that the parallelism can concern curved lines, which are thus equidistant over their entire length. The spikes can extend along rows, for example in the extension of the strips, when present, distributed over another region of the pad.

The pad can extend along a curved longitudinal axis, it being possible for said body to then exhibit, at least in a part intended to interact with the pad, a similar curved shape.

Such a shape can be ergonomic for holding in the hand and for using the device.

The body can extend from a lower end up to an upper end, along a longitudinal axis which is preferably curved, the body comprising a base portion comprising in particular a grasping part, including the lower end, and an upper portion comprising the upper end, a width of the base portion being greater than a width in the upper portion, the width of the upper portion preferably decreasing in the direction of the upper end.

The upper portion can comprise the part of the body which interacts with the pad and can comprise, if appropriate, at least a part of said complementary fixing protrusion when the pad is removably attached to the body.

The body can comprise, in a rear part, the grasping part and, in a front part opposite the rear part, said complementary fixing protrusion.

The body preferably exhibits an ergonomic shape, for example suited to the user, in particular in the grasping part.

Furthermore, the body advantageously comprises an opening for filling with the composition, the device comprising an at least partially removable cap arranged in order to close said filling opening. In this case and in the case where the body comprises a base portion exhibiting a lower end, the filling opening is preferably located at a lower end of the body, in particular at a lower end of the base portion of the body.

The filling opening can be defined by a skirt extending inside or outside the reservoir.

An adapting piece can be inserted between the cap and the body so as to make it possible to cause the cap and the body to interact with each other. For example, the adapting piece can be fitted by force or by snap fastening to the skirt extending outside the reservoir and defining the filling opening. The adapting piece can comprise a skirt extending substantially parallel to the skirt of the body and exhibiting an exterior threading capable of interacting with a corresponding threading present on an interior skirt of the cap in order to be able to close the filling opening.

The lower end of the device can be flat and sufficiently wide to be able to rest on a flat support and to make it possible to support the device without requiring the intervention of a person.

The body can comprise an air uptake orifice. Such an orifice can be provided with a valve, in particular with a valve similar to that which equips the feed orifice(s) for the departure of the cleansing composition.

When the body comprises an air uptake orifice provided with a valve, said valve can be arranged in order to open when the user stops pressing on the body so as to make possible the entry of air. The body can then return to its initial shape.

The shape and the dimensions of the device are preferably appropriate for satisfactory holding in the hand by the user. The total height of the body of the device can in particular be between 5 cm and 20 cm, in particular between 7 cm and 13 cm. The capacity of the reservoir can be between 3 cl and 20 cl approximately, in particular between 4 cl and 15 cl. The width of the device can be between 3 cm and 8 cm, in particular approximately 5 cm. The depth, from the front to the rear, of the device can be between 3 cm and 10 cm, for example, in particular between 7 and 9 cm, it being possible, of course, for this depth to depend on the pad which is attached to the body.

Composition As specified above, the composition of the device according to the invention combines at least all or part of a lysate, isolated or not, from a medium for the fermentation of a proteobacterium or a specific fraction of such a lysate, at least one mucopolysaccharide, at least one ancillary moisturizing agent, at least one electrolyte and water.

According to a particular embodiment, the composition of the device according to the invention, in particular a cosmetic and non-therapeutic composition, for the care, hygiene and/or cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails, in the form of an aqueous solution comprises at least:

(i) all or part of a lysate, isolated or not, from a medium for the fermentation of a non- pathogenic and aerobic proteobacterium or a (glyco)oligopeptide fraction isolated from such a lysate,

(ii) at least one mucopolysaccharide with a weight of greater than 10 kD,

(iii) at least one moisturizing agent distinct from (i) and from (ii) and chosen from glycerol, aminoglycerol, urea, their salts and their mixtures,

(iv) at least one electrolyte,

(v) water and,

(vi) if appropriate, at least one cosmetic active principle distinct from (i), (ii) and (iii).

According to another particular embodiment, the composition of the device according to the invention, in particular a cosmetic and non-therapeutic composition, for the care, hygiene and/or cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails, in the form of an aqueous solution comprises at least:

(i) all or part of a lysate, isolated or not, from a medium for the fermentation of a non- pathogenic and aerobic proteobacterium or a (glyco)oligopeptide fraction isolated from such a lysate, said proteobacterium being the bacterium Vitreoscilla filiformis and preferably the Vitreoscilla filiformis ATCC 15551 strain,

(ii) at least one carrageenan or one of its salts with a weight of greater than 10 kD,

(iii) at least one moisturizing agent distinct from (i) and from (ii) and chosen from glycerol, aminoglycerol, urea, their salts and their mixtures,

(iv) at least one electrolyte,

(v) water and,

(vi) if appropriate, at least one cosmetic active principle distinct from (i), (ii) and (iii). According to another particular embodiment, the composition of the device according to the invention, in particular a cosmetic and non-therapeutic composition, for the care, hygiene and/or cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails, in the form of an aqueous solution comprises at least:

(i) a (glyco)oligopeptide fraction isolated from a lysate from a medium for the fermentation of proteobacterium Vitreoscilla filiformis, in particular the Vitreoscilla filiformis ATCC 15551 strain,

(ii) at least one carrageenan or one of its salts with a weight of greater than 10 kD,

(iii) at least one moisturizing agent distinct from (i) and from (ii) and chosen from glycerol, aminoglycerol, urea, their salts and their mixtures,

(iv) at least one electrolyte,

(v) water and,

(vi) if appropriate, at least one cosmetic active principle distinct from (i), (ii) and (iii).

According to another particular embodiment, the composition of the device according to the invention, in particular a cosmetic and non-therapeutic composition, for the care, hygiene and/or cleansing of the skin and/or keratin fibers and/or mucus membranes and/or nails, in the form of an aqueous solution comprises at least:

(i) a (glyco)oligopeptide fraction isolated from a lysate from a medium for the fermentation of proteobacterium Vitreoscilla filiformis, in particular the Vitreoscilla filiformis ATCC 15551 strain,

(ii) at least one carrageenan or one of its salts with a weight of greater than 10 kD,

(iii) at least one moisturizing agent distinct from (i) and from (ii) and which is glycerol,

(iv) at least one electrolyte,

(v) water and,

(vi) if appropriate, at least one cosmetic active principle distinct from (i), (ii) and (iii).

According to one embodiment, the moisturizing agent is chosen from glycerol, aminoglycerol, urea, their salts and their mixtures.

According to another embodiment, the moisturizing agent comprises glycerol, aminoglycerol, urea, their salts and their mixtures.

According to another embodiment, the moisturizing agent consists in glycerol, aminoglycerol, urea, their salts and their mixtures. Lysate from medium for the fermentation of bacteria

A lysate commonly denotes a material obtained on conclusion of the destruction or dissolution of biological cells by a phenomenon termed cell lysis, thus bringing about the release of the intracellular and cellular biological constituents naturally contained in the biological cells under consideration.

Within the meaning of the present invention, the term lysate denotes all of the lysate obtained by lysis of the proteobacterium concerned. A lysate is thus formed of all of its intracellular biological constituents, in particular its metabolites and the constituents of the cell walls and membranes generated during its cell lysis.

Within the meaning of the invention, the term “metabolite” denotes any substance resulting from the metabolism of the microorganism under consideration according to the invention.

This cell lysis can be accomplished by various technologies, such as, for example, osmotic shock, thermal shock, by ultrasound, or under mechanical stress of centrifugation type. More particularly, this lysate can be obtained according to the technology described in the patent US 4 464 362.

As specified above, according to a specific embodiment, a composition of the device according to the invention contains all or only a part of a lysate from the fermentation medium or the biomass of a proteobacterium. Thus, a part of the lysate can be isolated from the whole lysate by a centrifugation method. It can, for example, be the supernatant of the lysate.

Mention may in particular be made, by way of illustration and without limitation of the nonpathogenic and aerobic proteobacteria suitable for the invention, of the bacterium/bacteria belonging to the Vitreoscilla sp genus, to the Sphingomonas sp genus or to the Pseudoalteromonas sp genus.

More specifically, the bacterium is a nonsynthetic filamentous bacterium, as defined according to the classification of Bergey's Manual of Systematic Bacteriology (Vol. 3, sections 22 and 23, 9th edition, 1989), belonging to the Vitreoscilla sp genus. More particularly, it is the bacterium Vitreoscilla filiformis .

In a specific embodiment, the bacterium is the Vitreoscilla filiformis strain, preferably the Vitreoscilla filiformis ATCC 15551 strain. In order to prepare the lysate of a bacterium of the Vitreoscilla sp genus, reference may be made in particular to the description of the patent application WO-A-94-02158 or of the patent application WO2008/138839.

According to another specific alternative form, the composition of the device according to the invention contains the lysate in a complete fermentation medium.

Within the meaning of the invention, the expression “lysate in a complete fermentation medium” means that the lysate is present, in the composition of the device according to the invention containing it, formulated in its original complete culture medium, which complete culture medium is the medium in which the bacteria were cultured until after the microbial growth phase which led to the use of the nutritive substrates initially present in the culture medium.

More specifically, this “complete fermentation medium” is understood to denote a medium resulting from the culture method which has been used for the growth and the cell lysis of the bacterium, said medium moreover not having been subjected to any additional handling targeted at separating and/or removing all or part of its nonaqueous constituents.

More particularly, the composition contains the lysate of microorganisms and all or part, in terms of amount, of the culture medium which served for the fermentation of said bacterium and in which its cell lysis was consecutively carried out (i.e. complete fermentation medium). This “complete” fermentation medium thus contains the cytoplasmic and cytosolic fractions, the cell wall fragments and the metabolites formed and/or released during the cell lysis of said microorganism and all of the biological entities capable of being generated and released spontaneously by the bacterium during its fermentation process and thus already present in the fermentation medium before the cell lysis of the latter.

Thus, in a specific embodiment, a composition of the device according to the invention contains the lysate of the Vitreoscilla filiformis strain, preferably the Vitreoscilla filiformis ATCC 15551 strain, in its complete fermentation medium. This lysate in complete medium can in particular be obtained according to the protocol described in the patent application EP 2 830 588.

According to yet another specific alternative form, a composition of the device according to the invention contains the (glyco)oligopeptide fraction isolated from a lysate of a biomass referred to also as medium for fermentation of a proteobacterium. Within the meaning of the invention, the expression (glyco)oligopeptide fraction covers a fraction containing at least one oligopeptide and/or at least one glycooligopeptide.

Within the meaning of the present invention, oligopeptide is understood to mean a heterooligopeptide and glycooligopeptide is understood to mean an oligopeptide covalently bonded to a mono- or oligocarbohydrate glycosyl radical, which is generally small in size. The weight of the glycosyl part of a glycooligopeptide can vary from 5% to 40% of the molecular weight of the glycooligopeptide assembly.

This oligopeptide fraction can be obtained via a method consisting in:

- the culturing of at least one proteobacterium on a fermentation medium under conditions conducive to the proliferation of said bacterium, and

- a centrifugation stage, and

- optionally adjustment of the pH, and

- the enzymatic digestion of the residue derived from the centrifugation of said bacteria in said fermentation medium.

The oligopeptide fraction within the meaning of the invention thus in particular denotes the fraction resulting from the digestion of the residue, resulting from the centrifugation, which is optionally adjusted to a given pH and subsequently treated by a carbohydrase enzyme and a protease enzyme.

In order to isolate the oligopeptide fraction of a proteobacterium/bacteria of a Vitreoscilla filiformis strain, reference may be made in particular to the description of the patent application WO2018/108973. More specifically, the oligopeptide fraction thus isolated is constituted of 30% to 50% of oligopeptides, between 1% and 10% of amino acids and of 10% to 20% of a mixture of oligosaccharides and of monosaccharides. Generally, the oligopeptides have a molecular weight of between 200 Da and 3 kDa, with a peptide distribution centered on the 200-800 Da zone. In particular, the oligopeptides are peptides between 2 and 7 amino acids on average. Glutamic acid, glutamine, histidine and arginine constitute at least 15% of said 1% to 10% of amino acids. Preferably, the oligosaccharides are chosen from stachyose, sucrose, glucose, fructose and trehalose.

Mucopoly s accharide Mucopolysaccharides are a class of polysaccharides, also called glycosaminoglycans, composed of aminosugars chemically bonded as repeat units to form compounds of unbranched linear polymer type.

These compounds are very particularly advantageous for contributing long- lasting moisturizing to the skin or to the scalp.

In particular, mucopolysaccharides are present in the compositions of the device according to the invention in a form distinct from a lysate.

Mucopolysaccharides with weights (that is to say molecular weights) of greater than 10 kD are more particularly suitable for the invention.

The mucopolysaccharides suitable for the invention can in particular be chosen from polysaccharides extracted from marine algae, in particular green, red or brown algae, and very particularly from carrageenans, alginates, ulvans, fucoidans, chitosan, chitin, their salts and their mixtures.

A composition of the device according to the invention can thus contain, as mucopolysaccharides, at least one alginate.

Alginic acid is a natural substance extracted from brown algae or from bacteria. Alginic acid forms water-soluble salts (alginates) with alkali metals or alkaline earth metals, such as sodium, calcium, potassium or lithium; (Ci-C8)alkylamines and (Ci- C8)alkylammoniums, such as methylamine, ethanolamine, diethanolamine or triethanolamine.

A composition of the device according to the invention can also contain, as mucopolysaccharides, at least one ulvan, one of its salts or extracts. Ulvans are sulfated anionic polysaccharides containing rhamnose which are obtained from green algae belonging to the order of the Ulvales. Ulvans are water-soluble sulfated anionic polysaccharides which are located in the cell wall. The ulvan according to the invention preferably originates from Ulva lactuca. The ulvan can be employed in the form of the extract called “OligoUlvan” and which can be obtained according to the patent FR 3 075 644.

A composition of the device according to the invention can also contain, as mucopolysaccharide, at least one fucoidan, one of its salts or extracts. Fucoidan, an algal extract, is a generic term denoting a polysaccharide which comprises sulfated fucose as saccharide component. It can in particular be the commercial product Matrigenics distributed by Codif.

A composition of the device according to the invention can also contain, as mucopolysaccharide, at least one (co)polymer of glucosamine chosen from chitosan and its salts. Chitosan is a polysaccharide composed of D-glucosamine and of N-acetyl-D- glucosamine. It can be employed as is or in the form of one of its salts, such as salts with a carboxylic acid comprising from 1 to 6 carbon atoms, such as acetic acid, thioglycolic acid, adipic acid, ascorbic acid, formic acid, glycolic acid and lactic acid. This chitosan can in particular be employed in the form of the commercial product Kionutrime distributed by Kytozyme.

In particular, a composition of the device according to the invention contains, as mucopolysaccharide, at least one carrageenan or one of its salts.

Carrageenans are sulfated and linear polysaccharides, comprising long galactan chains formed of disaccharide units, which constitute the cell walls of varied red algae, from which they can be obtained. Mention may be made, among these red algae, in a nonlimiting way, of Kappaphycus alvarezii, Eucheuma denticulatum, Eucheuma spinosum, Chondrus crispus, Betaphycus gelatinum, Gigartina skottsbergii, Gigartina canaliculata, Sarcothalia crispata, Mazzaella musceapellata, Mastnocariformatus cordiforme, Irocariformata cordiforme, Irrocariformata cordiforme, Mazzaella musceapusellatus and Irocariformatus cordiforme.

The carrageenans which are particularly suitable for the invention are the carrageenans of lambda, kappa or iota form, their hybrids and their mixtures in all proportions. Thus, a single carrageenan or a mixture of carrageenans may be concerned.

More specifically, these carrageenans are chosen from the following compounds:

Iota carrageenan;

Kappa carrageenan;

Lambda/kappa carrageenan mixture, in particular sold under the name Satiagel, and Lambda carrageenan.

The carrageenans of the present invention can be used in the acid form or in the salified form. The acceptable salts which can be mentioned in a nonlimiting way comprise the lithium, sodium, potassium, calcium, zinc and ammonium salts or the salts obtained with an organic base counterion, such as a primary, secondary or tertiary (Ci-C6)alkylamine, in particular triethylamine or butylamine, indeed even the basic form of amino acids, such as that of arginine and lysine, for example.

The molecular weight of the carrageenans of use for the present invention is generally between 10 x 10 ³ and 100 x 10 ⁶ Da and in particular between 15 x 10 ³ and 10 x 10 ⁵ Da.

The carrageenans which can be used in particular in the invention predominantly comprise lambda forms or are provided in the lambda form. The term “predominantly” means that the percentage of this type of chain in the composition of the product is greater than or equal to 50%, it being possible for this proportion to be greater than or equal to 80% in some embodiments.

The carrageenans derived from Chondrus crispus or Kappaphycus alvarezii are suitable in particular.

The carrageenans extracted from Chondrus crispus can in particular be those sold by Cargill under the respective names Satiagum UTC 30 Carrageenan lamda Cargill and Satiagum UTC 10 Carrageenan lamda Cargill.

A composition of the device according to the invention, in aqueous presentation formulation, can comprise from 0.01% to 5% and preferably from 0.02% to 0.1% by weight of mucopolysaccharide(s) and in particular of carrageenan(s), with respect to the total weight of the composition.

The mucopolysaccharide(s) according to the invention is (are) combined with at least one ancillary moisturizing agent in particular chosen from glycerol, aminoglycerol, urea, their salts and their mixtures.

The moisturizing agent is described as ancillary as it is distinct from a lysate or from a mucopolysaccharide as required according to the invention and described in detail above.

In particular, a composition of the device according to the invention comprises one or more mucopolysaccharide(s) and one or more ancillary moisturizing agents, especially in a moisturizing agent(s)/mucopolysaccharide(s) ratio by weight varying from 50 to 1000 and preferably from 100 to 500 and in particular from 200 to 400. The amount of ancillary moisturizing agent in a composition of the device according to the invention, in aqueous presentation formulation, can vary from 1% to 30%, preferably from 5% to 20%, by weight, with respect to the total weight of the composition.

Electrolytes

A composition of the device according to the invention additionally comprises one or more electrolytes.

Mention may more particularly be made, as electrolytes which can be used in the composition of the device according to the invention, of alkaline earth metal salts and in particular calcium salts, alkali metal salts and, for example, sodium and potassium salts, and also magnesium or manganese salts, and their mixtures.

The ions constituting these salts can be chosen, for example, from carbonates, bicarbonates, sulfates, phosphates, sulfonates, glycerophosphates, borates, bromides, chlorides, fluorides, nitrates, acetates, hydroxides or persulfates and also ions of a-hydroxy acids (citrates, tartrates, lactates, malates) or of fruit acids, ions of P-hydroxy acids (salicylates, 2-hydroxyalkanoates, n-alkylsalicylates and n-alkanoylsalicylates) or also ions of amino acids (aspartate, arginate, glycocholate, fumarate).

Generally, a composition of the device according to the invention contains at least a mixture of several electrolytes chosen from alkaline earth metal salts and in particular calcium salts, alkali metal salts and in particular sodium and potassium salts, and also magnesium or manganese salts and carbonates, bicarbonates, sulfates, phosphates, bromides, chlorides and fluorides.

Use may also be made of a natural mixture of electrolytes or of a mixture, the composition of which is close to that of a natural mixture, and in particular the powder mixtures marketed, such as the commercial products, powder from Bulk Powders® and electrolytes from Global Medics® to be diluted in demineralized water.

The electrolytes can be employed as is or in the form of a solution generally water or an aqueous medium containing them in the form of solutes, such as, for example, a seawater, a thermal water or a mineral water. In general, a mineral water is fit for consumption, which is not always the case for a thermal water. Each of these waters contains, inter alia, dissolved minerals and trace elements. The nature of its salts and their respective amounts are, of course, adjusted in order to be physiologically acceptable. Thus, for example, in the case where seawater is considered as source of electrolyte, it is employed in a form depleted in NaCl, indeed even desalified.

Aqueous medium

A composition of the device according to the invention comprises at least 50% by weight and in particular at least 80% by weight of an aqueous medium, with respect to its total weight.

This aqueous medium is or contains water and preferably spring, thermal or mineral water.

It can also be formed in part of seawater, which, as described in detail above, can be considered in a composition of the device according to the invention as source of electrolytes.

The thermal water or the mineral water used can be chosen, for example, from Vittel water, Vichy basin water, Uriage water, La Roche-Posay water, La Bourboule water, Enghien-les-Bains water, Saint-Gervais-les-Bains water, Neris-les-Bains water, Allevard- les-Bains water, Digne water, Maizieres water, Neyrac-les-Bains water, Lons-le-Saunier water, Eaux-Bonnes water, Rochefort water, Saint Christau water, Les Fumades water, Avene water and Tercis-les-Bains water.

According to one embodiment, a composition of the device according to the invention contains water and at least a mixture of several electrolytes chosen from alkaline earth metal salts and in particular calcium salts, alkali metal salts and in particular sodium and potassium salts, and also magnesium or manganese salts and carbonates, bicarbonates, sulfates, phosphates, bromides, chlorides and fluorides, in particular in a proportion of 0.1% to 20%, or preferably of 0.10% to 0.5%, with respect to its total weight.

According to a specific alternative form of the invention, the composition of the device according to the invention comprises: at least the oligopeptide fraction isolated from a lysate from a medium for the fermentation of a proteobacterium Vitreoscilla filiformis, in particular the Vitreoscilla filiformis ATCC 15551 strain, at least one mucopolysaccharide, in particular at least one carrageenan, at least one moisturizing agent chosen from glycerol, aminoglycerol, urea, their salts and their mixtures, at least desalified seawater, and water.

Mention may in particular be made, by way of illustration and without limitation of compositions of the device according to the invention, of the aqueous care, hygiene and/or cleansing compositions containing: at least the oligopeptide fraction isolated from a lysate from a medium for the fermentation of a proteobacterium Vitreoscilla filiformis, in particular the Vitreoscilla filiformis ATCC 15551 strain, approximately 0.05% by weight of at least one mucopolysaccharide, in particular at least one carrageenan, with respect to its total weight, approximately 15% by weight of at least one moisturizing agent chosen from glycerol, aminoglycerol, urea, their salts and their mixtures, with respect to its total weight, approximately 10% by weight of desalified seawater, with respect to its total weight, and approximately 74% by weight of water, distinct from the seawater, with respect to its total weight.

Surfactants

A composition of the device according to the invention can additionally contain one or more surfactants, in particular for their detergent properties.

This surface-active component is, of course, chosen, on the one hand, for its compatibility with the other ingredients present in the composition and, on the other hand, in order not to prejudice the qualities desired for the claimed composition.

In particular, this surface-active component is chosen from biosurfactants.

Surfactants of lauryl sulfate type may also be suitable. However, when the compositions according to the invention are very particularly dedicated to being comparable to “biobased” products, bio surfactants are favored.

For the record, biosurfactants are surfactants of microbial origin. They are produced and excreted by several specific bacterial strains, in particular those of the Pseudomonas and Bacillus genera, for example. Several types of bio surfactants exist, including glycolipids (including rhamnolipids) and lipopeptides very particularly suitable for the invention.

These biosurfactants have the advantage both of possessing an effectiveness as surfactant and of being biodegradable and thus advantageously nonpersistent in the environment, one of the objectives pursued according to the present invention.

Such surfactants are in particular described in the paper by D.K. De Santos et al., 18 March 2016, International Journal of Molecular Sciences.

Mention may in particular be made, by way of illustration and without limitation of the surfactants suitable for the invention, of spiculisporic acid (S acid) and its salts, in particular that sold under the name S acid by Iwota Chemical, rhamnolipids, in particular that sold under the name Rhamnolipid L by Kaneka, sophorolipids, in particular that sold under the name Sopholiance S by Solinace-Givaudan, and surfactants, in particular those sold under the trade name Surfactin Sodium Salt by Evonik.

In a known way, care and cleansing compositions and thus those according to the invention can contain, besides the compounds required according to the invention, one or more adjuvants conventional in the cosmetics field (fragrance, fillers, odor absorbers, coloring materials, and the like) and one or more cosmetic or therapeutic active principles other than the active principles required according to the invention.

More particularly, this or these other cosmetic or therapeutic active principles can be chosen from sunscreens; desquamating or exfoliating agents, such as, for example, compounds derived from salicylic acid or their salts; depigmenting agents; propigmenting agents; alpha-hydroxy acids; antibacterial agents; agents for combating free radicals; agents for combating pollution; retinoids; extracts of fungi, of plants other than algae or of bacteria other than proteobacteria; hydrolyzed, partially hydrolyzed or nonhydrolyzed proteins; enzymes, hormones, yeast extracts, vitamins and their derivatives, flavonoids and isoflavones, and their mixtures.

They can in particular be chosen from enzymes, in particular proteases of use for the digestion of corneocytes, such as, for example, papain from BASF or Patel Papain Industries, beta-glucosidases, such as those of almonds, such as cellobiose sold by Novozymes or Aspergillus niger beta-glucosidase from Megazymes, subtilisin, in particular such as those sold by Novozyme, alcalase, such as in particular collupulin sold by DSM and bromelain from Enzybel.

The amounts of these various adjuvants are those conventionally used in the field under consideration and, for example, can vary from 0.01% to 5% and preferably from 0.1% to 1% by weight of the total weight of the composition.

Presentation formulations

The compositions of the device according to the invention are suitable for application by the external topical route. To do this, they are provided at the time of the application in a liquid preparation form and in particular in the form of aqueous or aqueous/alcoholic solutions, of solutions or dispersions of the lotion, gel or serum type, indeed even of emulsions of liquid consistency. When the composition is an emulsion, the proportion of the fatty phase can range from 0.5% to 30% by weight and preferably from 1% to 20% by weight, with respect to the total weight of the composition. The oils, the waxes or the emulsifiers, indeed even coemulsifiers, used in the composition in the emulsion form are chosen from those conventionally used in the cosmetics field.

The compositions of the device according to the invention can be used on the skin or the keratin fibers, including the scalp, head hair, eyelashes, eyebrows or other keratin materials, such as the nails, or the mucus membranes, in particular in the form of a hygiene product, for example in the form of a product for cleansing the skin, mucus membranes and/or head hair or the beard, in particular in the form of a product for cleansing and/or removing makeup from the skin (of the face and/or of the body), in the form of a shower product, in the form of a shampoo and conditioner, in the form of a shaving product, in the form of a rinse-off mask, in the form of an exfoliating product (also called desquamating or deep cleansing agent), both for the face and for the body or for the hands, after addition of exfoliating particles.

Brief description of the drawings

A better understanding of the invention can be obtained on reading the detailed description which will follow of nonlimiting implementational examples thereof and on examining the appended drawing, in which: [Fig 1] figure 1 represents, in schematic exploded view and in perspective, an example of a device in accordance with the invention,

[Fig 2] figure 2 represents, in front view, schematically, the device of figure 1 fitted with a first pad,

[Fig 3] figure 3 represents, in side view, schematically, the device of figure 2,

[Fig 4] figure 4 represents, in front view, the device of figure 1 fitted with a second pad,

[Fig 5] figure 5 represents, in side view, schematically, the device of figure 4,

[Fig 6] figure 6 represents, schematically and in isolation, in front view, the body of the device of figure 1,

[Fig 7] figure 7 represents, schematically, in side view, the body of figure 6,

[Fig 8] figure 8 represents, schematically, in bottom view, the body of figure 6,

[Fig 9] figure 9 is a view in longitudinal section and in perspective of the body of the device of figure 7,

[Fig 10] figure 10 is a view in longitudinal, schematic and partial section of the device of figure 1, once fitted,

[Fig 11] figure 11 represents, in isolation, schematically and in perspective, the second pad of figures 4 and 5,

[Fig 12] figure 12 is a schematic view of the interior of the pad of figure 11,

[Fig 13] figure 13 is a schematic side view along XIII of the pad of figure 11,

[Fig 14] figure 14 is another schematic side view along XIV of the pad of figure 11,

[Fig 15] figure 15 represents, schematically and in perspective, in isolation, the first pad of figures 2 and 3,

[Fig 16] figure 16 schematically represents the interior of the pad of figure 15,

[Fig 17] figure 17 represents, schematically, in side view along XVII, the pad of figure 15,

[Fig 18] figure 18 represents, schematically, in another side view along XVIII, the pad of figure 15,

[Fig 19] figure 19 represents, schematically and in isolation, in longitudinal section, the cap of the device of figure 1, [Fig 20] figure 20 represents, in perspective, schematically and partially, an example of a device according to the invention during its use,

[Fig 21] figure 21 is a schematic magnified view of the item XXI of figure 20,

[Fig 22] figure 22 represents, in isolation, schematically and in perspective, an example of a pad on the inside of the latter,

[Fig 23] figure 23 represents, schematically in exploded front view in perspective, another example of a device according to the invention, without the pad,

[Fig 24] figure 24 represents the device of figure 23 assembled, in schematic rear view and in perspective, with the pad,

[Fig 25] figure 25 represents, in isolation, schematically and in perspective, another example of a body which can form part of the device according to the invention,

[Fig 26] figure 26 represents, in isolation, schematically and in perspective, another example of a body which can form part of the device according to the invention,

[Fig 27] figure 27 represents, in isolation, schematically and in perspective, another example of a body which can form part of the device according to the invention, and

[Fig 28] figure 28 schematically represents an example of a process for the manufacture of the device according to the invention, and

[Fig 29] figure 29 schematically represents the body movement protocol for cleansing taken into consideration for testing the effectiveness of the device according to the invention.

Detailed description

In the continuation of the description, identical elements or identical functions carry the same reference sign. For the purposes of conciseness of the present description, they are not described taking into account each of the figures, only the differences between the embodiments being described.

In the figures, the actual proportions have not always been respected, for the sake of clarity.

A description will be given of a first embodiment in the light of figures 1 to 19. There has been illustrated, in figure 1, an example of a device 1 for cleansing the skin and/or keratin fibers, such as head hair or the beard, and/or mucus membranes and/or nails, according to the invention, comprising a body 2 intended to contain the composition as defined above. The body 2 comprises at least one wall 3 delimiting a volume forming a reservoir 4 for housing the composition. At least a part of the wall 3 is elastically deformable. The body 2 again comprises at least one feed orifice 5, in the example illustrated five feed orifices 5, which are through orifices, in order to make possible the departure of the cleansing composition outside the body 2.

The device 1 again comprises at least one valve 6, in this example five valves 6, each one capable or not of sealing one of the feed orifices 5.

The device 1 again comprises at least one pad 10, in this example two pads 10, respectively 10a and 10b, intended to be in contact, for the purpose of cleansing, with the skin and/or keratin fibers. In the example illustrated, the pad 10a is intended to be in contact with the skin, whereas the pad 10b is intended to be in contact with keratin fibers.

Each pad 10 is capable of being removably attached to the body 2 and exhibits an exterior surface 15 comprising cleansing protrusions 7 comprising, in this example, spikes 16.

In the example illustrated, at least one spike 16, referenced 16a, as visible more exactly in figures 11 and 15, in this example five spikes 16a, exhibits a passage 17 emerging on a dispensing opening 18 for the dispensing of the cleansing composition. This spike 16a is positioned on the pad 10 so that, when the pad 10 is attached to the body 2, the feed orifice

5 is positioned with regard to the spike 16a in order to make possible the dispensing of the cleansing composition from the reservoir 4 up to the dispensing opening 18.

As can be seen in figure 1 and as will be described below, the spikes 16 of the pad 10a are at least in part different from the spikes 16 of the pad 10b.

Still in figure 1, the presence in the device 1 of retaining elements 11 forming collars intended to make possible the interaction between the valves 6 and the seals 20 is displayed. Each retaining element 11 is thus arranged in order to cause the valve 6 to interact with a corresponding feed orifice 5. Each retaining element 11 forms a collar with an exterior diameter substantially equal to that of the feed orifice 5 and with an interior diameter substantially equal to the exterior diameter of a part of the valve 6, so as to support the valve

6 on the outside and to interact on the inside with the feed orifice 5. This arrangement is visible more particularly in figure 10.

Each valve 6 is configured so as to make possible the retention of the cleansing composition in the absence of pressure exerted on the wall 3 in its elastically deformable part and the departure of cleansing composition when a pressure is exerted on the elastically deformable part of the wall 3. As visible in figure 1 and figure 10, each valve 6 exhibits, in this example, a base 21 of annular shape with an end 22 of the base exhibiting a greater diameter than the remainder of the base. The valve 6 also exhibits a seal 20 attached to the base 21, the seal 20 comprising, in this example, two elastically deformable membranes interacting in the absence of pressure so as to prevent any passage of the cleansing composition and defining, between them, an opening when a pressure is exerted on the elastically deformable part of the wall 3 of the body 2 in order to make possible the passage of the cleansing composition. As visible in figure 10, each feed orifice 5 comprises a first feed orifice portion 5a, on the side of the reservoir 4, and a second feed orifice portion 5b, with a greater diameter than that of the first portion 5a, extending in the axial extension of the latter. A shoulder 5c connects the first portion 5a to the second portion 5b. The second portion 5b houses the valve 6. The end 22 of the base 21 of the valve 6 rests axially against the shoulder 5c. The retaining element 11 inserted into the second portion 5b of the feed orifice itself rests axially against the end 22 and transversely against the remainder of the base 21.

The device 1 again comprises a cap 12, visible in figure 1 and in isolation in figure 19, intended to interact with a filling opening 50, visible in figures 8 and 9, in order to make possible the filling of the reservoir 4 with cleansing composition.

The body 2 is made entirely of an elastically deformable material in the example illustrated, in particular of biobased high-performance elastomer, that is to say obtained by a process starting from plant material, in this example constituted by a thermoplastic elastomer (TPE) of the bioTPE range sold by NaturePlast, being made of a single part by injection molding of material. Likewise, the pads 10 are made entirely of at least one elastically deformable material, also made of biobased high-performance elastomer, in this example constituted by a thermoplastic elastomer of the range sold under the name Dryflex® Green by Hexpol, each also made of a single part by injection molding of material.

Figures 2 and 3 show the device 1 assembled with the pad 10a fitted to the body 2. It is seen in particular in this figure that, in this example, the pad 10a and the body 2 extend along a curved longitudinal axis X. Figures 4 and 5 show the device 1 assembled with the pad 10b fitted to the body 2. The pad 10b also extends along the curved longitudinal axis X. The pads 10a and 10b exhibit overall shapes and an exterior outline which are substantially similar. For the removable attachment of the pad 10a or of the pad 10b to the body 2, as visible in figures 16 and 12, respectively, the pads 10a and 10b comprise, on a lower surface 25 opposite the exterior surface 15, a fixing protrusion 26.

For its part, as visible in particular in figures 1, 6, 7, 8 and 9, the body 2 exhibits, on a front part 27, a complementary fixing protrusion 28 capable of interacting, in this example mechanically, with the fixing protrusion 26 of the pads 10a and 10b for the removable attachment of these pads 10a and 10b, alternately, to the body 2.

In the example illustrated, the complementary fixing protrusion 28 forms a flange extending toward the exterior of the body 2 and exhibiting an outline 35, substantially bean-shaped, which is closed around a zone 36. The outline 35 of the flange exhibits a shape similar to that of the exterior outline 29 of the pads 10.

In the example illustrated, tongues 31 are formed at the longitudinal ends of the pad 10 to make the pad 10 easier to grasp, in particular in order to remove it from the body 2.

The openings 32 formed in the pad 10b or 10a are displayed in figures 12 and 16, respectively. These openings 32 continue through the passage 17 made in the five spikes 16a in order to make possible the passage of the cleansing composition.

The passage 17 of each spike 16a, as visible in particular in figures 11 and 15, emerges on the dispensing opening 18 for the dispensing of the cleansing composition. The passage 17, in the example illustrated, passes through the spike 16a from an end 33 of the spike 16a, connected to the exterior surface 15 of the pad 10, up to a free end 34 of the spike 16a. The free end 34 comprises the dispensing opening 18 for the departure of the cleansing composition.

The feed orifices 5 are positioned on the body 2 in this example, as visible in particular in figure 1 or 6, in a row 40 provided in order to spread out substantially at the middle of the pad 10 when the latter is attached to the body 2. In the example illustrated, the pad 10 extends along a curved longitudinal axis X. In this case, the row 40 is also substantially curved, following the axis X with a curvature similar to a curvature of the curved longitudinal axis X of the pad 10. The spikes 16a are positioned on the pads 10a and 10b also in a row 39, in an identical way, as visible, for example, in figures 2, 4, 11 and 15, in order to make possible good correspondence between feed orifices 5 and passages 17. As visible in figures 11, 13 and 14, the pad 10b intended for the cleansing of keratin fibers comprises only curved spikes 16, including the spikes 16a, being all sloping. These spikes 16 are, in this example, configured in order to imitate the papillae of the tongue of a feline, in this example constituted by a cat. In this example, the spikes 16 are curved by being directed toward a single imaginary point located at a distance from the pad 10b. The spikes 16 are positioned evenly over the pad 10b over the exterior surface 15, in rows, being distributed over the entire exterior surface 15 of the pad. The spikes 16 exhibit similar lengths and similar diameters and shapes, apart from the five spikes 16a positioned in the central row 39 on the pad 10b. The spikes 16 positioned close to the outline 29 have a smaller diameter than that of the spikes 16 positioned close to the row 39. The spikes can have a Shore hardness of between 60 and 100 Shore A, it being possible for this hardness in particular to be greater than the hardness of the papillae of a cat. The length can be greater than that of the papillae of a cat by at least 20%, in particular by at least 40%, for example by 60%. Such a hardness and such a length can make it possible efficiently to comb or brush human head hair and/or to massage the scalp.

As visible in figures 15, 17 and 18, the pad 10a intended for the cleansing of the skin comprises spikes 16, denoted 16b, of a first shape and spikes 16, denoted 16c, of a second shape, independently of the spikes 16a making possible the dispensing of the cleansing composition. The spikes 16b and spikes 16c differ in this example in the diameter, the spikes 16b having a greater diameter than the spikes 16c. The spikes 16c are found close to this outline 29, whereas the spikes 16b are closer to the spikes 16a. All the spikes 16 of the pad 10a are rectilinear, form a point, having a substantially conical shape, and extend substantially orthogonally to the pad 10a, being positioned parallel to one another, in particular in the form of rows which are parallel to one another and which are curved in order to follow the exterior outline 29 of the pad 10a, as visible in particular in figure 15 or 2.

The pad 10a is not entirely covered with spikes 16, in this example. It comprises a region 45, located in an upper part of the pad 10a as visible in figure 2, comprising cleansing protrusions 7 forming strips 46 positioned orthogonally to the exterior surface 15. Thus, during the cleansing of the skin, massaging or rubbing can be carried out on the skin while applying the cleansing composition using the spikes 16 and then scraping can be carried out with the strips 46 in order to remove, for example, the cleansing composition and/or dead cells and other impurities. As visible in particular in figures 1, 6, 7 and 9, the body 2 extends in the example illustrated from a lower end 44 up to an upper end 41 along the curved longitudinal axis X. The body 2 comprises a base portion 42 including the lower end 44 and an upper portion 43 comprising the upper end 41. The width of the base portion 42 is greater than the width of the portion in the upper portion 43 in this example, the width of the upper portion 43 decreasing in the example illustrated in the direction of the upper end 41.

Furthermore, the body 2 exhibits, besides the front part 27 comprising the complementary fixing protrusion 28, a rear part 47, opposite the front part 27, defining an ergonomic grasping part. The reservoir 4 is thus delimited by the wall 3, which also forms in part the grasping part of the device.

There is, in this example, no distinct handle.

It is not departing from the scope of the invention if a distinct handle is provided in the body, in addition to the reservoir, in particular if the shape of the reservoir is not ergonomic. In this case, the body can comprise a handle forming a loop on the upper face of the reservoir, in which handle the hand might be engaged and thus press on the reservoir in order to release the cleansing composition instead of gripping the reservoir in the absence of a distinct handle.

The body 2 comprises an opening 50 for filling with the cleansing composition, as visible in figures 8 and 9. In order to close this filling opening 50, the cap 12, visible in isolation in figure 19, is provided. The cap 12 comprises an exterior skirt 51 arranged to cover the wall 3 of the body 2 in the base portion 42 of the body 2. Furthermore, the cap 12 comprises internally, in a central part, a stud 52 intended to interact internally with a skirt 53 defining the filling opening 50, as visible in figure 9, by snap fastening or threading, so as to seal in leaktight manner the filling opening 50. It is seen, in figure 9, that the wall 3 is set back in the lower part 54 in order to make it possible for the exterior skirt 51 of the cap 12 to become flush without excess thickness when the cap 12 is attached to the body 2, as visible in figures 2 to 5.

In order to use the device 1 according to the invention, the reservoir 4 is filled with the composition.

A pad 10 is rendered integral with the body 2, for example the pad 10a for the cleansing of the skin, using the complementary protrusions 26 and 28. Pressure is applied to the rear part 47, which is elastically deformable, of the body 2 so as to create an excess pressure targeted at opening the seals 20 of the valves 6 and at making it possible for the cleansing composition to pass from the reservoir 4 up to the dispensing openings 18 at the end of the spikes 16a. Rubbing or massaging of the skin is carried out, for example in a circular manner, in order to cleanse it. The strips 46 make it possible subsequently to scrape the skin in order, for example, to remove all or part of the resulting cleansing composition and of the impurities and dead cells.

For the cleansing of keratin fibers, the same procedure is followed, except that the pad 10b instead of the pad 10a is rendered integral and the spikes 16 are brought into contact with the keratin fibers so as to apply, to the latter, a cleansing composition and to brush them or to comb them or to massage the scalp.

The invention makes it possible to efficiently cleanse the skin or keratin fibers using the device 1 provided according to the invention without requiring the contribution of water or requiring only a slight contribution of water, which can prove to be of use in the case of lack of water or for a person of little or no mobility.

In an alternative form, it is possible to fill the reservoir with a flexible bottle surmounted by a cone, which would be inserted into a hole made in the upper face of the reservoir, optionally formed into a skirt made of elastomer which opens when the bottle of cleansing composition is inserted therein and which closes when it is removed.

Another embodiment of the pad 10 has been represented in figures 20 to 22, in which embodiment the protrusion 26 comprises an edge 30 extending uniformly over the entire outline of the pad, as visible in figure 22.

Furthermore, in this embodiment, the retaining elements 11 are made as a single part with the pad. The cleansing composition S is displayed in figures 20 and 21 exiting at the end 34 of the spikes 16a.

Figures 23 and 24 illustrate another embodiment of the device 1 according to the invention. This device 1 differs from that illustrated in figures 1 to 19 in that the body 2 comprises an air uptake orifice 68, positioned in this example in the rear part 47 in the upper portion 43 of the body 2, as visible in figure 24. Such an air uptake orifice 68 is furnished with a valve 69, in this example with a valve similar to the valve 6 which equips the feed orifices 5 for the departure of composition. In this case, the valve 69 is arranged in order to open when the user stops pressing on the body 2, so as to make possible the entry of air into the reservoir 4. The body 2 can then return to its initial shape.

Furthermore, in the embodiment of figures 23 and 24, the filling opening 50 is defined by the skirt 53 extending outside the reservoir 4, downwards.

An adapting piece 55 is inserted between the cap 12 and the body 2 so as to make it possible to cause the cap 12 and the body 2 to interact with each other. The adapting piece 55 is fitted by force or by snap fastening to the skirt 53 extending outside the reservoir 4. The adapting piece 55 itself comprises a skirt 56 extending substantially parallel to the skirt 53 of the body 2 and exhibiting an exterior threading capable of interacting with a corresponding threading present on the central stud 52 of the cap 12 in order to be able to close the filling opening 50. As visible in figure 24, the adapting piece 55 is provided to be flush with the surface of the body 2, like the stopper 12.

Three other possible forms of body 2 have been represented in figures 25 to 27.

In figure 25, the rear part 47 forming the grasping part substantially reproduces the contours of the hand and of the fingers.

Figure 26 illustrates a body 2 of rounded general shape in its rear part 47, with a hollow.

Finally, figure 27 illustrates a body 2 forming an elbow in the direction of the rear part 47.

Various stages of an example of a process for the manufacture of a body 2 for a device 1 according to the invention have been represented in figure 28.

The first stage 60 consists in taking an image, in this example in scanning, using an appliance O, a hand M of a user. In a second stage 61, the images resulting from the first stage 60 are processed, so as to define a suitable profile of wall 3 of the body 2. In a third stage 62, the body 2 is produced by polymer material additive manufacturing. In a fourth stage 63, the desired body 2, suited to the user, is thus obtained.

It should be noted that, in this method, as well as for the examples of figures 25 to 27, the front part 27 and the complementary protrusion 28 always remain identical and are not adapted, in order for the body 2, whatever its shape, to be able to be assembled with any pad 10.

Of course, the invention is not limited to the examples which have just been described. In particular, the shape of the body 2 can be modified without departing from the scope of the invention.

The fixing and complementary protrusions (26 and 28 respectively) can be different from those which were described above while making it possible to interact with each other for the removable attachment of the pad 10 to the body 2, without departing from the scope of the invention.

The cleansing protrusions 7 may not comprise spikes 16. The dispensing opening 18 may not be formed in a spike 16 but be present at the exterior surface 15 of the pad 10 or be formed in a cleansing protrusion 7 other than a spike. In this case, the dispensing opening 18 is nevertheless provided to be positioned on the pad 10 so as to make possible the departure of cleansing composition from the reservoir 4 up to the dispensing opening 18 by passing through the feed orifice 5.

The diameter of a spike 16 can be between 0.5 mm and 3 mm, preferably being around 1 mm approximately.

In an alternative form, the presence of the pad is necessary to free the valves and to make it possible to open them under the pressure of the cleansing composition.

In another alternative form, the pad forces the opening of the valves, so that, according to the pad placed on the body, the valves can open more or less.

In a specific embodiment, the device 1 does not comprise a valve 6.

In one embodiment, the pad is irremovably attached to the body.

In an alternative form, the body does not comprise an elastically deformable wall.

Materials and methods

A first test is targeted at evaluating the cleansing power of the device according to the invention on a stain formed of a mixture of charcoal particles and sebum described in detail below and which is deposited on a skin model: 7.6 pl/cm ² of a mixture of avocado oil (from Expanscience, virgin oil) (greasy skin) + charcoal (Sigma- Aldrich, active charcoal) at 2% by weight of the mixture (dirty skin) is applied with a syringe pipette with inclination of the skin in order to distribute the solution homogeneously at ambient T and ambient humidity. The evaluation is carried out on a rectangular zone of 10 cm by 4 cm. To do this, the synthetic skin is cut out or a zone of this size is defined on the forearm. A second test is targeted at evaluating its cleansing power toward a foundation (L'Oreal Paris, Accord Parfait), which is applied on a forearm of a tester in order to form a layer of foundation having a respective length and width of 10 cm and 4 cm. The deposit of this foundation is formed by pressing three times on the dose, which are equivalent to 1.5 g, using three fingers in order to distribute the foundation homogeneously at ambient T and ambient humidity.

Skin models:

Sample of smooth synthetic skin (punch diameter 5 cm) from Biolax: Bioskin Plate K520 thickness 2 mm attached with double-sided Scotch tape to a cork sheet covered with aluminum paper.

Human skin forearm internal face of a tester.

It is carried out 5 minutes after application of the composition to be cleansed off.

100 ml of the washing solution to be tested are incorporated in the reservoir of the device 1 according to the invention as illustrated in figure 2.

The washing solution is dispensed by the dispensing opening 18 formed in the spikes 16a of the device 1, brought into contact with the skin to be cleansed and there is applied, with the pad 10, a body movement for recovery /rubbing in the form of 9 passes, 3 zigzags toward the left, 3 zigzags toward the right, with the spikes 16, and then 3 straight passes with the strips 46, according to the protocol represented schematically in figure 29.

The effectiveness of the cleansing is assessed by visual observation of the state of the skin.

This effectiveness is characterized on a performance scale ranging from 5+ to 1+ (5+: the state of the skin observed is clean and perfectly decent; 1+: the state of the skin is not clean and the stain is still observed).

The amount of stain (first test) or foundation (second test) recovered during the cleaning operation is determined by subtracting from the weight of the device with the washing solution before cleansing, to its weight obtained after cleansing. It is subsequently expressed as percentage with respect to the amount of stain or foundation deposited before cleansing. Film-forming quality of the composition

The expert grades on a scale ranging from 5+ to 5- (5-: no film-forming effect noticed, rough skin; 5+: significant film- forming effect). The grading is carried out 3 minutes after the application of the product.

Determination of the sensory experience

The expert grades, on a scale ranging from 5+ to 5- (5-: no sensory effect noticed; 5+: significant sensory effect), the intensity of the sensation noticed after the application of the product. The grading is carried out 3 minutes after the application of the product.

Example 1

Preparation of an oligopeptide fraction isolated from a V. filiformis lysate

The composition of the complete nutrient medium for the culturing of V. filiformis is described in detail in table 1.

[Table 1]:

In order to obtain the oligopeptide fraction, the procedure as described below is carried out.

The initial strain of V. filiformis is obtained from the ATCC (strain 15551). The biomass is obtained by fermentation in an industrial bioreactor of 3000 effective liters; the composition of the culture medium is given above. During the culturing, the pH is kept constant (7.00), as is the temperature (26°C) and the dissolved oxygen (0.5%). The culturing is stopped when the solids content reaches 0.6% and the glucose content reaches 0.035%. The V. filiformis strain is fermented in its complete culture medium.

Everything is centrifuged and the residue is treated by enzymatic digestion.

For the enzymatic digestion, first a Viscozyme® carbohydrase sold by Novozyme is introduced in an amount of 0.2% w/w over a period of time of 3 h at a temperature of 55°C, the pH is adjusted to and maintained at 4.5 by citric acid, then a Sumizyme AP protease sold by Novozyme is introduced in an amount of 0.04% w/w over a period of time of 3 h at a temperature of 55°C and the pH is maintained at 4.5 after adjustment by addition of citric acid, if necessary.

Subsequent to this second enzymatic digestion, the medium is neutralized to pH 7 using a sodium hydroxide (NaOH) solution. The enzyme is inactivated by heating the medium at a temperature of 90°C for 15 minutes.

The neutralized and inactivated medium is clarified by successive filtrations, first with a 10 pm bag filter and then a K2 filter.

A stage of spray drying the clarified complete reaction medium is carried out with a Buchi mini spray dryer B-290 with the following parameters: Pump 30%; Inlet temperature 160°C; Outlet temperature 85°C; Aspirator 95; Q flow 35. The oligopeptide fraction is subsequently recovered in the powder form and is used as is in the examples which follow. of a device in accordance with the invention

A formula A is prepared by simple mixing under a vortex at ambient temperature, that is to say varying from 18 °C to 25 °C, of the 3 ingredients described in detail in table 2. [Table 2]

A formula B is prepared by heating the water to 70°C and by incorporating into it, with stirring, the two other ingredients described in detail in table 2, until they have dissolved.

[Table 3]

The two formulae are subsequently mixed to form the expected composition. Example 3

Preparation of compositions suitable for the device according to the invention

The compositions 2a to 2f described in detail below in table 4 were prepared according to the protocol described in detail in example 2. [Table 4]

Example 4

Test of the effectiveness of cleansing of a composition with the device according to the invention A first test is carried out in order to assess the effectiveness of a device according to the invention for the removal of polluting particles and of a surplus of sebum on a synthetic skin model and on the skin of a forearm.

To do this, the composition 2a of the example is introduced in a proportion of 2 ml into the reservoir of the device 1 according to the invention and dispensed over the skin according to the protocol described in detail above and illustrated in figure 29.

By way of comparison, a control micellar solution, namely the Crealine H2O Bioderma solution, is applied using a Demak’Up cotton pad, by depositing 2 ml of micellar water on the skin and by then passing the cotton pad (Control A). Only three scrapings are carried out with the cotton pad.

The control micellar solution Crealine H2O (Bioderma) comprises the following components: water, PEG-6 caprylic/capric triglycerides, fructooligosaccharides, mannitol, xylitol, rhamnose, cucumber fruit extract, PEG, cetrimonium bromide, EDTA.

In order to assess the effectiveness of the interaction between a composition and a device 1 according to the invention, the following control tests B and C are additionally carried out: Control B: the composition 2a is used with the device according to the invention but in a dissociated manner, i.e. 2 ml of the composition are applied on the skin, followed by the use of the device. After application of the composition to the skin, only three scrapings are carried out with the spike-comprising part of the device.

Control C: 2 ml of the micellar solution of the control A are used with the device (excluding the composition) according to the invention but in a dissociated manner, i.e. 2 ml of the solution are applied on the skin, followed by the use of the device. After application of the solution to the skin, only three scrapings are carried out with the spike-comprising part of the device.

Control D: 2 ml of the micellar solution of the control A are introduced into the reservoir of the device 1 according to the invention and dispensed over the skin according to the protocol described in detail above and illustrated in figure 29.

Results on synthetic skin

The cleansing with the composition 2a applied with the device according to the invention on synthetic skin is better visually than when the composition 2a is applied in a way dissociated from the device (Control B) and much better visually than Controls A, C and D employing the micellar solution.

The results obtained on the skin of a forearm are reported in table 5 below. [Table 5]

The cleansing with the composition 2a applied with the device according to the invention on human skin of a forearm is better visually than when the composition 2a is applied in a way dissociated from the device (Control B) and much better visually than Controls A, C and D employing the micellar solution. Furthermore, it emerges from these results that the best performances are obtained when a washing composition in accordance with the invention is applied using a device according to the invention at the surface of the skin zone to be cleansed. The composition and the device clearly interact together to provide effective cleansing. A second test is carried out in order to assess the effectiveness of a device according to the invention for the removal of a foundation applied on a skin zone of a forearm.

The composition 2a of the example is introduced into the reservoir of the device 1 according to the invention and dispensed over the skin of a forearm according to the protocol described in detail above and illustrated in figure 29.

The Crealine H20 Bioderma micellar solution is tested in parallel as control washing solution under the same applicational conditions. (Control A)

The control micellar solution Crealine H2O (Bioderma) comprises the following components: water, PEG-6 caprylic/capric triglycerides, fructooligosaccharides, mannitol, xylitol, rhamnose, cucumber fruit extract, PEG, cetrimonium bromide, EDTA.

In order to assess the effectiveness of the interaction between a composition and a device 1 according to the invention, the following control tests B and C are additionally carried out. Control B: the composition according to the invention is used with the device according to the invention but in a dissociated manner, i.e. 2 ml of the composition are applied on the skin, followed by the use of the device.

Control C: the micellar solution of the control A is used with the device 1 according to the invention but in a dissociated way, i.e. 2 ml of the solution are applied on the skin, followed by the use of the device.

The results obtained are reported in table 6 below.

[Table 6]

It emerges from these results that the best performances are obtained when a washing composition in accordance with the invention is applied using a device 1 according to the invention at the surface of the skin zone to be cleansed. The composition and the device clearly interact together to provide effective cleansing.