Title:
DEVICE AND METHOD FOR ELECTROCHEMICAL DETECTION
Document Type and Number:
WIPO Patent Application WO/2017/014828
Kind Code:
A3
Abstract:
A microfluidic, electrochemical device for detecting a genetic material is described, including: one or more cellulosic layers comprising at least one of a hydrophilic test zone and a hydrophilic sample deposition zone in fluid communication with each other; one or more amplification agents selected for amplifying a genetic material and embedded in the hydrophilic test zone or the sample deposition zone; one or more binding agents embedded in the test zone or the sample deposition zone and selected for binding the amplified genetic material to result in a change of the concentration of a signaling chemical, wherein the signaling chemical is either embedded in the test zone or the sample deposition zone prior to the binding or is a newly generated product of the binding; and an electrode assembly comprising one or more electrodes in fluidic contact with the test zone and configured to interact with the signaling chemical to result in a current change readable by an electrochemical reader.
Inventors:
TSALOGLOU MARIA-NEFELI (US)
NEMIROSKI ALEX (US)
CHRISTODULEAS DIONYSIOS (US)
CAMCI-UNAL GULDEN (US)
CONNELLY JOHN T (US)
FERNANDEZ-ABEDUL MARIA TERESA (ES)
WHITESIDES GEORGE M (US)
NEMIROSKI ALEX (US)
CHRISTODULEAS DIONYSIOS (US)
CAMCI-UNAL GULDEN (US)
CONNELLY JOHN T (US)
FERNANDEZ-ABEDUL MARIA TERESA (ES)
WHITESIDES GEORGE M (US)
Application Number:
PCT/US2016/032706
Publication Date:
March 16, 2017
Filing Date:
May 16, 2016
Export Citation:
Assignee:
HARVARD COLLEGE (US)
UNIV SOUTHAMPTON (GB)
UNIV SOUTHAMPTON (GB)
International Classes:
G01N27/26; G01N27/327; G01N27/416
Domestic Patent References:
| WO2014152825A1 | 2014-09-25 |
Foreign References:
| US20140183059A1 | 2014-07-03 | |||
| US20140042038A1 | 2014-02-13 | |||
| US20120181184A1 | 2012-07-19 |
Other References:
MAXWELL ET AL.: "Paper-based electroanalytical devices for accessible diagnostic testing", MRS BULLETIN, vol. 38, 12 April 2013 (2013-04-12), pages 309 - 314, XP055369444
NEMIROSKI ET AL.: "Universal mobile electrochemical detector designed for use in resource-limited applications", PROC NATL ACAD SCI USA, vol. 111, 4 August 2014 (2014-08-04), pages 11984 - 11989, XP055211323
NEMIROSKI ET AL.: "Universal mobile electrochemical detector designed for use in resource-limited applications", PROC NATL ACAD SCI USA, vol. 111, 4 August 2014 (2014-08-04), pages 11984 - 11989, XP055211323
Attorney, Agent or Firm:
SCOZZAFAVA, Mary, Rose et al. (US)
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